소아 삼출성 중이염이 약물 치료에 영향을 미치는 인자에 관한 연구

김영기,정현수,함태영 인제대학교 1994 仁濟醫學 Vol.15 No.2

삼출성 중이염의 예후와 관련된 인자를 알아보기 위하여 외래를 방문한 51명의 환자를 대상으로 난치성과 관련이 있는 요인을 조사해 본 결과, 과거의 중이수술 유무, 동반된 부비동염 등이 약물 치료에 대한 반응의 중요한 예후인자라고 생각되었다. It has been clarified that multiple factors are implicated in the otitis media with effusion which is common in children. This prospective study was performed to evaluate the factors related to the prognosis of the otitis media with effusion. History, physical examination, impedance audiometry, and radiologic examination were taken. Oral antibiotics (Augmentin or cefixime) were given to all patients orally for 3 months. Myringotomy and V -tube insertion was done in the patients who had no response after 3 months of medication. The factors implicated in the prognosis of otitis media with effusion were analysed. The significant risk factors were present inthose wish previous myringotomy or V-tube insertion, presence of mucopurulent rhinorrhea and total hazziness on PNS X-rays, but not to adenoid vegetaton, eardrum retraction, age, sex, bilaterality and presence of recurrence. Therefore, the presence of previous middle ear surgery and concomitant sinusitis were important prognostic factors in treatment of the otitis media with effusion medically.

청국장의 생리활성물질에 관한 연구 동향과 유전공학적 접근을 통한 기능성 강화

김기연,함영태 중앙대학교 유전공학연구소 2003 遺傳工學硏究論集 Vol.16 No.1

최근에 청국장에 포함된 생리활성물질이 각종 만성퇴행성질환에 효과가 있음이 과학적으로 밝혀짐에 따라, 청국장을 기능성 건강식품으로 개발하고자하는 연구가 활발히 진행되고 있다. 청국장의 생리활성물질들은 크게 대두에 포함되어 있는 성분에서부터 청국장이 발효과정 중 protease, lipase, amylase에 의해 대두로부터 분해 되는 산물, 그리고 청국장의 발효균주 자체가 생산하는 산물들로 나누어 볼 수 있다. 따라서 본 총론에서는 현재까지 과학적으로 밝혀진 청국장에 포함된 기능성 생리활성물질 및 그 특성과 이들 생리활성물질에 대한 최근의 연구동향에 대해 알아보고, 이미 건강기능식품으로서의 자격이 충분한 청국장에 대한 최근의 연구동향에 대해 알아보고, 이미 건강기능식품으로서의 자격이 충분한 청국장에 대두로부터 청국장 발효 과정 중 균주의 가수분해 효소의 발현 양을 조절해서 생리활성물질을 다량 생산하거나 균주자체가 가지고 있는 생리활성물질을 유전공학적 응용을 통하여 대량생산 등의 유전공학적으로 기능성을 강화할 수 있는 방법을 모색하고자 한다.

Filamentous fungi Aspergillus species에서 Phytase 고발현 균주의 선별

이응석,함영태 中央大學校 食糧資源硏究所 1997 食糧資源硏究所 論文集 Vol.18 No.1

Phytase production of Aspergillus species were influenced by nitrogen source. Ammonium ions, especially ammonium phosphate (NH4H2PO4), were markedly superior to nitrate for the Phytase production. For the isolation of phytase gene, the culture of Aspergillus species (A. oryzae) were maintained on PSM media containing ammonium phosphate. After 4 days, mycellia were harvested by centrifugation and isolated total RNA. cDNAs were synthesized from total RNA by RT-PCR. The Phytase gene was amplified with the primers designed from A. ficuum sequence. 1.2 and 2.0 kb of the amplified DNA fragments by secondary PCR and subcloned in pT7Blue-T vector. Aspergillus species (A. oryzae and A. ficuum) were treated with 254㎚ of UV light for the screening of high phytase producing mutant strains, comparative to wild type on PSM media, containing ammonium phosphate. Two UV mutant strains in A. oryzae and three, in A. ficuum were isolated. These mutants are 1.5 to 2.0 times better phytase-production than Wild type.

곰팡이의 원형질체를 이용한 항생제 저항성 연구

김기모,이경아,함영태 中央大學校 食糧資源硏究所 1994 食糧資源硏究所 論文集 Vol.6 No.1

A. oryzae와 T.inflatum의 wild-type host에 dominant selectable marker인 phleomycin resistancy를 가지는 E. coli Tn5의 ble gene과 hygromycin B에 대한 저항성을 나타내는 E. coli hph gene의 사용 가능성을 분석키 위하여 phleomycin과 hygromycin B를 농도별 (5㎍∼200㎍ of antibiotic/㎖ of media)로 곰팡이의 원형질체에 적용하여 그 저항성을 비교 분석하였다. Lysing enzyme과 β-glucuronidase를 germinated spore(??)에 처리하여 A. oryzae에서는 4.3∼4.8×10⁴protoplast를 얻은 반면, T.inflatum에서는 1.8∼2.5×10²protoplast만을 얻었다. A. oryzae의 hygromycin B 저항성을 농도 50∼200㎍/㎖ of media에서 조사하여 본 결과, hygromycin 200㎍에서도 강한 저항성을 보여 주었고, T.inflatum에서는 항생물질 농도 25㎍/㎖에서 60%의 세포수 감소를 보이며, 75㎍/㎖에서90% 이상의 세포수 감소를 보여주었다. Phleomycin 저항성에 대한 실험에서는 A.oryzae에서는 phleomycin 50㎍/㎖ of media에서 50% 정도의 저항성을 보여 주고 있으며 , 50㎍/㎖농도에서는 85% 정도의 세포수 감소가 관찰되었고, T.inflatum에서는 hleomycin 농도 5㎍/㎖에서도 강한 생장 억제효과가 나타나는 것을 보여 주었다. Antibiotic-resistancy on fungi, Aspergillus oryzae and Tolypocladium inflatum, were analyzed by using fungal protoplasts and antibiotics, hygromycin. Fungal protoplasts were prepared from the germinated spored treated with lysing enzyme and β-glucuronidase. 4.3∼4.8×10⁴protoplasts of A . oryzae per ㎖ were obtained from ?? spores whereas 1.8∼2.5×10²protoplasts of T. inflatum per ㎖ were harvester. A. oryzae protoplasts have strong resistancy against the hygromycin B, up to 20 ㎍/㎖ of media. Less than 10% of the initial population of T. inflatum protoplasts were survived on minimal media containing 75㎍/㎖ hygromycin B. In phleomycin-resistancy, the population of A. oryzae portoplasts were killed up to 50% of the initial population on minimal media containing 50㎍/㎖ antibiotic and up to 80% on the complex media with the same amount of antibiotic. No survived colonies of T. inflatum protoplasts were observed on the minimal media containing phleomycin, 5 to 20㎍/㎖.

Poster Session 2 : Oral administration of high molecular weight poly-gamma-glutamate promotes anti-tumor activity in C57BL/6 mice

( Tae Young Lee ),( Jung Ho Hahm ),( Jong Soo Lee ),( Chung Park ),( Moon Hee Sung ),( Chul Joong Kim ),( Har Young Poo ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

Chemoprevention of Helicobacter pylori-associated Gastric Carcinogenesis in a Mouse Model; Is It Possible?

Hahm, Ki-Baik,Song, Young-Joon,Oh, Tae-Young,Lee, Jeong-Sang,Surh, Young-Joon,Kim, Young-Bae,Yoo, Byung-Moo,Kim, Jin-Hong,Ha, Sang-Uk,Nahm, Ki-Taik,Kim, Myung-Wook,Kim, Dae-Yong,Cho, Sung-Won Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.1

Although debates still exist whether Helicobacter pylori infection is really class I carcinogen or not, H. pylori has been known to provoke precancerous lesions like gastric adenoma and chronic atrophic gastritis with intestinal metaplasia as well as gastric cancer. Chronic persistent, uncontrolled gastric inflammations are possible basis for ensuing gastric carcinogenesis and H. pylori infection increased COX-2 expressions, which might be the one of the mechanisms leading to gastric cancer. To know the implication of long-term treatment of antiinflammatory drugs, rebamipide or nimesulide, on H. pylori-associated gastric carcinogenesis, we infected C57BL/6 mice with H. pylori, especially after MNU administration to promote carcinogenesis and the effects of the long-term administration of rebamipide or nimesulide were evaluated. C57BL/6 mice were sacrificed 50 weeks after H. pylori infection. Colonization rates of H. pylori, degree of gastric inflammation and other pathological changes including atrophic gastritis and metaplasia, serum levels and mRNA transcripts of various mouse cytokines and chemokines, and NF-${\kappa}B$ binding activities, and finally the presence of gastric adenocarcinoma were compared between H. pylori infected group (HP), and H. pylori infected group administered with long-term rebamipide containing pellet diets (HPR) or nimesulide mixed pellets (HPN). Gastric mucosal expressions of ICAM-1, HCAM, MMP, and transcriptional regulations of NF-${\kappa}B$ binding were all significantly decreased in HPR group than in HP group. Multi-probe RNase protection assay showed the significantly decreased mRNA levels of apoptosis related genes and various cytokines genes like IFN-$\gamma$, RANTES, TNF-$\alpha$, TNFR p75, IL-$1{\beta}$ in HPR group. In the experiment designed to provoke gastric cancer through MNU treatment with H. pylori infection, the incidence of gastric carcinoma was not changed between HP and HPR group, but significantly decreased in HPN group, suggesting the chemoprevention of H. pylori-associated gastric carcinogenesis by COX-2 inhibition. Long-term administration of antiinflammatory drugs should be considered in the treatment of H. pylori since they showed the molecular and biologic advantages with possible chemopreventive effect against H. pylori-associated gastric carcinogenesis. If the final concrete proof showing the causal relationship between H. pylori infection and gastric carcinogenesis could be obtained, that will shed new light on chemoprevention of gastric cancer, that is, that gastric/cancer could be prevented through either the eradication of H. pylori or lessening the inflammation provoked by H. pylori infection in high risk group.

The Report on the Medical Voluntary Activities for Cleft Lip and Palate in Vietnam by the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons

Tae-Hoon Hahm,Hyo-Keun Shin,Jong-Ryul Kim,Dong-Mok Ryu,Sun-Youl Ryu,Kyoung-Won Kim,Young-Wook Park,Young-Soo Jung 대한구순구개열학회 2010 대한구순구개열학회지 Vol.13 No.1

대한악안면성형재건외과학회는 2004년도부터 매년 인도적 차원에서 의료 환경이 낙후된 Vietnam의 cleft lip and palate 환자들을 치료하기 위해 무료로 해외 진료 봉사 활동을 해왔다. 초창기 해외 진료봉사 활동 당시 베트남 의료 기술 발달이 미비하여 준비해야 할 것들이 많았으나 이 후 베트남의 경제발전과 함께 의료 시설 및 의료 기술이 발달함에 따라 준비 품목 등이 간단명료해지고 현지 지원 및 수술 준비도 원활히 진행되었다. 이러한 무료 의료 봉사 활동을 계기로 개발도상국의 낙후된 의료 시설 및 의료 기술 발전, 의료 혜택을 받지 못한 구순 구개열 환자의 정상적 안모 및 기능 회복, 조건 없는 의료 봉사 활동을 통한 민간외교 효과 및 양국간의 우호 증진 등의 성과가 있었다. 대한악안면성형재건외과학회에서는 2009년도 역시 베트남 National Institute of Odonto-stomatology 에서 11월17일부터 26일까지 cleft lip and palate 무료수술진료단(단장 신효근)을 보내 수술봉사 활동을 펼치고 돌아 왔다. 일반적인 구순구개열 뿐만 아니라 양측성 안면열(Bilateral Facial Cleft), 사경(Torticollis) 등의 희귀 기형을 포함하여 수술을 진행하였으며 총 31례의 수술을 성공적으로 마쳤으며 지난 6년 동안의 성과와 함께 이에 대한 보고를 하고자 한다.

Poster Presentations : Helicobacter pylori and Stress Augment the Gastric Mucosal Lesion ?

(Tae Young Oh),(Ju Mi Kim),(Seul Min Choi),(Byoung Ok Ahn),(Won Bae Kim),(Young Bae Kim),(Ki Baik Hahm) 서울시립대학교 산업기술연구소 2002 국제학술SYMPOSIUM논문집 Vol.10 No.1

Detection of Functional(nonorganic)Hearing Loss

Hahm, Tae Young 대한이비인후과학회 1971 대한이비인후과학회지 두경부외과학 Vol.14 No.2

Chemoprevention of Helicobacter pylori-associated Gastric Carcinogenesis in a Mouse Model : Is It Possible?

Hahm, Ki Baik,Song, Young Joon,Oh, Tae Young,Lee, Jeong Sang,Surh, Young-Joon,Kim Young Bae,Yoo, Byung Moo,Kim, Jin Hong,Han, Sang Uk,Nahm, Ki Taik,Kim, Myung-Wook,Kim, Dae Yong,Cho, Sung Won 한국생화학분자생물학회 2003 BMB Reports Vol.36 No.1

Although debates still exist whether Helicobacter pylory infection is really class I carcinigen or not, H. pylori has been known to provoke precancerous lesions like gastric adenoma and chronic atrophic gastritis with intestinal metaplasia as well as gastric cancer, Chronic persistent, uncontrolled gastric inflammations are possible basis for ensuing gastric carcinogenesis and H. pylori infection increase COX-2 expressions, which might be the one of the mechanisms leading to gastric cancer. To know the implication of long-term treatment of antiinflammatory drugs, rebamipide or nimesulide, on H. pylori-associated gastric carcinogenesis, we infected C57BL/6 mice with H. pylori, expecially after MNU administration to promote carcinogenesis and the effects of the long-term administration of rebamipide or nimesulide were eva,uated. C57BL/6 mice werre sacrificed 50 weeks after H. pylori infection. Colonization rates of H.pylory, degree of gastric inflammation and other pathological changes including atrophic gastitis and metaplasia, serum levels and mRNA transcripts of various mouse cytoknes and chemokines, and NF-κ]B binding activities, and finally the presence of gastric adenocarcinoma were compared between H. pylori infected group (HP), and H.pylori infected group administrated with long-term rebamipide containing pellet diets (HPR) or nimesulide mixed pellets (HPN). Gastric mucosal expressions of ICAM-1, HCAM, MMP, and transcriptional regulations of Nf-κB binding were all significantly decreased in HPR group than in HP group. Multi-probe RNase protection assay showed the significantly decreased mRNA levels of apoptosis related genes and various cytokines genes like IFN-r, RANTES, TNF-α, TNFR p75, IL-1β in HPR group. In the experiment designed to provoke gastric cancer through MNU treatment with H.pylori infection, the incidence of gastric carcinoma was not changed between HP and HPR group, but significantly decreased in HPN group, suggesting the chemoprevention of H. pylori-associated gastric carcinogenesis by COX-2 inhibition. Long-term administration of antiinfalmmatory drugs should be considered in the treatment of H. pylori since they showed the molecular and biologic advantages with possible chemopreventive effect against H. pylori-associated gastric carcinogenesis. If the final concrete proof showing the causal relationship between H. pylori infection and gastric carcinogenesis. If the final concrete proof showing the causal relationship between H. pylori infection and gastric carcinogenesis could be obtained, that will shed new light on chemoprevention of gastirc cancer, that is, that gastric cancer could be prevented through either the eradication of H. pylori or lessening the inflammation provoked by H. pylori infection in high risk group.