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간호대학생이 지각하는 임상실습지도자의 교수효율성과 임상실습만족도의 관계
황현아,김희진,김예지,이규희,이영롱,박성희,손수빈 이화여자대학교 간호과학대학 2012 이화간호학회지 Vol.- No.46
Purpose: The purpose of this study was to investigate the relationship between teaching effectiveness and clinical practice satisfaction among nursing students. Method: The subjects of this study were 107 junior and senior nursing students in E university. Data were collected using self-reported questionnaire including general and practicum related characteristics, teaching effectiveness of clinical instructors and clinical practice satisfaction from September 12 to September 21, 2011. Collected data were analyzed by SPSS 19 program using t-test, ANOVA with Scheffe test, and Pearson`s correlation coefficients. Result: The mean score of teaching effectiveness was 3.35(±.51), and mean of clinical practice satisfaction was 3.19±.47. There were significant differences of teaching effectiveness of clinical instrutor by satisfaction of overall clinical practicum(F=8.332, p<.001), satisfaction of practice hour(F=3.230, p=.044), and satisfaction of major(F=9.883, p<.001). There were significant differences of clinical practice satisfaction by grade(t=2.274, p=.025), motive of choosing nursing science as a major(F=3.329, p=.007), satisfaction of overall clinical practicum(F=17.437, p<.001), satisfaction of practice hours(F=9.925, p<.001), and satisfaction of nursing major(F=12.748, p<.001). Relationship between teaching effectiveness of clinical instructor and clinical practice satisfaction showed positive correlation(r=.704, p<.001). Conclusion: In this study, teaching effectiveness of clinical instructor was related with clinical practice satisfaction. Therefore, we should consider improving teaching effectiveness of clinical instructor to improve clinical practice satisfaction.
Acupuncture inhibits liver injury induced by morphine plus acetaminophen through antioxidant system
Lee, Young Joon,Zhao, Rong Jie,Kim, Young Woo,Kang, Su Jin,Lee, Eun Kyung,Kim, Nam Jun,Chang, Suchan,Kim, Jin Mook,Lee, Ji Eun,Ku, Sae Kwang,Lee, Bong Hyo Elsevier 2016 European journal of integrative medicine Vol.8 No.3
<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>Morphine (MP) and acetaminophen (APAP), widely used-pain relievers and antipyretics, are known to induce hepatotoxicity. Acupuncture, a traditional therapy in Asia, is used for various reasons including detoxification. This study aimed to examine whether acupuncture exerts protective effects against MP+APAP-induced hepatic damage.</P> <P><B>Methods</B></P> <P>Male Sprague-Dawley rats received chronic MP, withdrawal, and APAP. Thereafter, blood and liver were taken. Acupuncture was performed once daily. Asparte aminotransferase (AST) and alanine aminotransferase (ALT) levels were measured, and percentages of abnormally decreased-hepatocyte regions, mean liver cell counts, and mean inflammatory cell numbers infiltrated on hepatic parenchyma were examined. In addition, antioxidant effects were evaluated based on liver lipid peroxidation malondialdehyde (MDA) and glutathione (GSH) contents, superoxide dismutase (SOD) and catalase (CAT) activities with the number of immunopositive hepatocytes against nitrotyrosine (NT) as a marker of inducible nitric oxide synthase (iNOS) related-oxidative stresses and 4-hydroxynonenal (4HNE) as a marker of lipid peroxidation.</P> <P><B>Results</B></P> <P>Significant elevations of AST and ALT were noted of MP or APAP. They also induced an increase in MDA contents as well as decreases in GSH levels and activities of SOD and CAT activity. A centrolobular decrease in hepatocytes along with degenerative changes of hepatocytes were also observed, and increases of NT and 4HNE immunoreactive hepatocytes were shown. These hepatocellular damages were more severe with the combination of MP+APAP. However, these MP+APAP-induced hepatic damages were significantly inhibited by acupuncture.</P> <P><B>Conclusion</B></P> <P>This study suggests that acupuncture may have a protective effect against the MP+APAP-induced hepatic damage through the amelioration of antioxidant defense systems.</P>
Lee, Dae Hong,Eun Jin Kang,Hee Jin Lee,Hee Bong Seok,Chang Wook Jo,Cho Rong Park,Myoung Gi Kang,Mi Ja Seo,Young Nam Youn 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.05
The multicolored Asian ladybird beetle, Harmonia axyridis, which demonstrates typical genetic polymorphism in its elytra color patterns. Early studies have color polymorphism in terms of geographical clines while a few investigated temporal populations in Coccinellidae. Nevertheless, note that geographical and temporal morph variation does not always correspond to what is expected from thermal and industrial adaption theories. A recent study of transformation and RNAi of the ladybird beetle, however, there is yet no evidence to indicate the variation is genetic or environmental factors. Here we describe a relatively new molecular fingerprinting technique, amplified fragment length polymorphism (AFLP). Because we think that color polymorphism in Coccinellidae is affected by genetic polymorphism. In total 38 markers were scored from which some markers were polymorphic. Supsequent UPGMA cluster analysis revealed 3 major group of Harmonia axyridis populations. But for strains that are more genetically similar, different primer combinations may be need to generate enough polymorphic marker.
Combination of honokiol and magnolol inhibits hepatic steatosis through AMPK-SREBP-1 c pathway
Lee, Ju-Hee,Jung, Ji Yun,Jang, Eun Jeong,Jegal, Kyung Hwan,Moon, Soo Young,Ku, Sae Kwang,Kang, Seung Ho,Cho, Il Je,Park, Sook Jahr,Lee, Jong Rok,Zhao, Rong Jie,Kim, Sang Chan,Kim, Young Woo SAGE Publications 2015 Experimental biology and medicine Vol.240 No.4
Transcriptional regulation of Egr-1 by overexpression of Phospholipase C-γ1
Lee, Young Han,Shin, Sun Young,Kim, Jae-Rong,Kim, Jung Hye,Bae, Sun Sik,Kim, Myung-Jong,Ryu, Sung Ho,Suh, Pann-Ghill 이화여자대학교 세포신호전달연구센터 2000 고사리 세포신호전달 심포지움 Vol. No.2
The early growth response 1(Egr-1) gene product is a transcription factor that contains three zinc-finger motifs in the DNA-binding domain and preferentially binds to GC-rich regulatory elements. The importance of Egr-1 in the regulation of cell proliferation and tumor formation was demonstrated in various experiments that showed that stable overexpression of Egr-1 inhibited DNA synthesis and tumorigenicity in various model cells. Phospholipase C(PLC)-γ1 plays a central role in cellular proliferation by triggering the formation of two second messengers, DAG and IP₃. Recently, it has been reported that overexpression of PLC-γ1 in murine 3Y1 fibroblasts leads to transforming activities. To explore the role of PLC-γ1 overexpression in tumorigenesis, we examined the effects of PLC-γ1 overexpression on tumor suppressor Egr-1 gene induction. Egr-1 mRNA was induced within 30 min of BCS treatment in vector transfected control cells(3Y1/vec), while completely suppressed in PLC-γ1 transfected 3Y1 fibroblasts(3Y1/γ1). In contrast, c-myc mRNA level was enhanced after BCS treatment in 3Y1/γ1 cells as compared with that in 3Y1/vec cells. Using human Egr-1 5' flanking deletion constructs, we demonstrated that a distal Serum Response Element(SRE) is necessary to suppress Egr-1 induction by PLC-γ1. EGF-and PMA-induced Egr-1 transcriptional activity is suppressed by PLC-γ1 in a dose-dependent manner, and constitutively active mutant of PLC-γ1 inhibited dominant active Ras- or MEK1-induced Egr-1 transcriptional activity. In addition, constitutively active mutant of PLC-γ1 was able to inhibit dominant active Ras- or MEK1-mediated SRF DNA binding activity. Taken together, these findings indicate that PLC-γ1 overexpression can regulate the Egr-1 gene induction via controlling the SRF DNA binding activity.
Cho Rong Han(한초롱),Ji Young Lee(이지영),Dongki Kim(김동기),Hyo Young Kim(김효영),Se Jin Kim(김세진),Seokjoon Jang(장석준),Yoon Hee Kim(김윤희),Do Youn Jun(전도연),Young Ho Kim(김영호) 한국생명과학회 2013 생명과학회지 Vol.23 No.10
17α-estradiol (17α-E₂)의 에폽토시스 유도활성에 미치는 종양억제단백질 p53의 조절효과를 조사하고자, 17α-E₂에 의해 유도되는 에폽토시스 현상들을 인체 대장암 세포주 유래 클론인 HCT116 (p53+/+) 및 HCT116 (p53-/-) 세포에서 비교하였다. HCT116 (p53+/+) 및 HCT116 (p53-/-) 세포를 17α-E₂ (2.5~10 μM)로 처리하거나 혹은 HCT116 (p53+/+) 및 HCT116 (p53-/-) 세포를 10 μM 17α-E₂로 시간 별로 처리한 결과, HCT116 (p53+/+)에 있어서는 세포독성과 에폽토시스-관련 sub-G₁ peak의 비율은 처리농도와 시간에 의존적으로 나타났다. 그러나 HCT116 (p53-/-) 세포의 경우는 이러한 현상이 미약하게 나타났다. 17α-E₂에 의해 유도되는 비정상적 유사분열방추사 형성, 중기판 염색체 배열의 미완성, 이에 따른 유사분열정지(G₂/M arrest) 등의 현상은 HCT116 (p53+/+) 및 HCT116 (p53-/-) 세포에서 유사한 수준으로 나타났다. 이에 반해, 17α-E₂에 의해 유도되는 Bak과 Bax의 활성화, 미토콘드리아의 막전위 상실(Δψm loss), 그리고 PARP 분해 등의 현상은 HCT116 (p53-/-) 세포에 비해 HCT116 (p53+/+) 세포에서 훨씬 높은 수준으로 확인되었다. 아울러 17α-E₂로 처리된 HCT116 (p53+/+) 세포에서 확인되는 p53 (Ser-15)의 인산화 및 p53 수준의 증가와 일치하여, 세포 내의 p21및 Bax 수준도 현저히 증가하였다. 이때 17α-E2로 처리된 HCT116 (p53-/-) 세포에서는 p21 및 Bax의 발현수준이 매우 낮았다. 한편, 에폽토시스 억제단백질인 Bcl-2 단백질수준은 HCT116 (p53-/-) 세포에 비해 HCT116 (p53+/+) 세포에서 다소 낮았으나, 이러한 Bcl-2 단백질 수준은 17α-E₂ 처리 후에도 크게 변화하지 않는 것으로 나타났다. 이러한 결과들은 17α-E₂ 처리에 의해 유도되는 에폽토시스 유도 경로의 구성원들의 변화, 즉 비정상적 유사분열방추사 형성 및 이에 따른 유사분열정지(G2/M arrest), 뒤이은 Bak 및 Bax의 활성화, 미토콘드리아의 막전위 상실, 그리고 이에 수반되는 caspase cascade 활성화 및 PARP 분해로 진행되는 에폽토시스 현상들 중에서, Bak 및 Bax의 활성화 단계가 종양억제단백질 p53의 에폽토시스 증진 활성에 의해 양성적으로 조절되는 작용 타켓임을 보여준다. The regulatory effect of the tumor-suppressor protein p53 on the apoptogenic activity of 17α-estradiol (17α-E₂) was compared between HCT116 (p53+/+) and HCT116 (p53-/-) cells. When the HCT116 (p53+/+) and HCT116 (p53-/-) cells were treated with 2.5~10 μM 17α-E₂ for 48 h or with 10 μM for various time periods, cytotoxicity and an apoptotic sub-G₁ peak were induced in the HCT116 (p53+/+) cells in a dose- and time-dependent manner. However, the HCT116 (p53-/-) cells were much less sensitive to the apoptotic effect of 17α-E₂. Although 17α-E₂ induced aberrant mitotic spindle organization and incomplete chromosome congregation at the equatorial plate, G₂/M arrest was induced to a similar extent in both cell types. In addition, 17α-E₂-induced activation of Bak and Bax, Δψm loss, and PARP degradation were more dominant in the HCT116 (p53+/+) than in the HCT116 (p53-/-) cells. In accordance with enhancement of p53 phosphorylation (Ser-15) and p53 levels, p21 and Bax levels were elevated in the HCT116 (p53+/+) cells treated with 17α-E₂. The HCT116 (p53-/-) cells exhibited barely or undetectable levels of p21 and Bax, regardless of 17α-E₂ treatment. On the other hand, although the level of Bcl-2 was slightly lower in the HCT116 (p53+/+) than in the HCT116 (p53-/-) cells, it remained relatively constant after the 17α-E₂ treatment. Together, these results show that among the components of the 17α-E₂-induced apoptotic-signaling pathway, which proceeds through mitotic spindle defects causing mitotic arrest, subsequent activation of Bak and Bax and the mitochondria-dependent caspase cascade, leading to PARP degradation, 17α-E₂-induced activation of Bak and Bax is the upstream target of proapoptotic action of p53.
Evaluation of Entomopathogenic Fungi for the Control of Hard Tick, Haemaphysalis longicornis
Mi Rong Lee,Tae Young Shin,Sihyeon Kim,Jong Cheol Kim,Se Jin Lee,So Eun Park,Dongwei Li,Jae Su Kim 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.04
The hard tick, Haemaphysalis longicornis (Ixodida: Ixodidae) is one of the vectors of severe fever with thrombocytopenia syndrome virus (SFTSV) causing in humans, however little consideration was given to the biological control of the pest. Therefore, this study provides a screening method to select entomopathogenic fungi, having high virulence against H. longicornis. The virulence test was conducted by dipping method with a conidial suspension (1×107 conidia/ml). As a result, cadavers of H. longicornis infected with entomopathogenic fungi were obtained by this method. Based on this results, we selected several isolates having high virulence to H. longicornis. We suggest that, selected isolates in this study can be used for the control of H. longicornis.