심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

김영랑 시의 정신세계

강영미(Kang Young-mi) 한국시학회 2004 한국시학연구 Vol.- No.10

Kim Young-rang has been known as poet who commanded elaborated type, meter, soft tone harmony of voice. Manufacture do poetry which have self-regulating structure through conscious calculation and materialize own inside sensuously. And his interest about shi-jo and pansori is reflected in production of poetry. He creates four line poetry criticizing existent poetry style(shi-jo and pansori and so on)in poetry´s circles in the 1930s that form of free poetry catches seat already, and plays a variation four line poetry learning forms and groped new modernistic poetry form. He established peculiar sensitivity of individual into compensation of shape and create chard free verse of new form. These features that shows in poetry form that defend self from colony actual broken external order is revealed can be seen. It is because construct space of imagination and found psychological pupil of the eye in to close loss persimmon formed on inside looking away actuality that can not accomplish own dream. Therefore, that appears in his poetry sensual and the trillion brazier fortune worlds can see that is product of injustice mind to look away colony actuality. Kim Young-rang did not compromise in colony actuality and could keep own inside to be upright through writing such poetry.

미치광이풀 모상근 배양에서 Tropane Alkaloid 생산성 증진을 위한 최적 생물학적 엘리시터 선발

정희영,강승미,강영민,김용덕,양재경,정영관,최명석 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Scopolamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supematant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.

남성화를 보이는 여성에서 발견된 난소의 Steroid Cell Tumor 1예

조인호,정대훈,박영미,서영진,손영실,정철회,강영미,정수전,김영남,이경복,성문수,김기태 인제대학교 2006 仁濟醫學 Vol.27 No.-

Steroid cell tumor is a rare ovarian sex cord-stromal tumor which accounts for 0.1% of all ovarian tumors. Until now, only 4 cases have been reported in domestic literatures. Steroid cell tumor often secrets testosterone and presents virilization in adult women or precocious puberty in children. Treatment is often performed by surgical removal, adjuvant chemotherapy and radiation, but completely accepted treatment was not existed. We experienced a case of steroid cell tumor, which was manifested by typical virilization in a 43-year old patient, who was previously performed hysterectomy and unilateral oophorectomy. So, we present with a brief review of the literatures.

Z정형 명세어를 이용한 클래스 스키마의 유도 및 프로토타이밍

강미형,조영석 동국대학교 경주대학 1999 東國論集 Vol.18 No.-

Software development cost and efforts can be considerably reduced if errors are detected and corrected in the early stages of software development life-cycle. For this purpose, formal languages are frequently recommended in specifying requirements. Object-oriented paradigm is considered as an effective means to build easily manageable software. Therefore, scientists and software engineers have made constant efforts in order to model object classes using Z. Notations and grammar of Z are usually extended to overcome the limitations of traditional Z resulting reduced chances to be applied in the real world programming. In this paper, our focus has made to derive class schemata from the traditional Z without programming. In this paper, our focus has made to derive class schemata from the traditional Z without an extension. Especially, three operators in Z, hiding operator, quantification operator, and projection operator were mainly applied to specify object classes. Fundamental characteristics of object-oriented paradigm are described using class schemata. In oder to prove object classes be successfully derived from class schemata, a prototype was built that converts a class schema into the object-oriented model. Lex and YACC are used for lexical and syntactic analyses, respectively. Further research would be conducted to resolve the automated type and access level assignments. Less important, but still essential, elements of C++ language must also be considered to be described by Z.

급성 췌장염을 동반한 임신성 급성 지방간 1 예

강미선;박상훈;박지영 서희영;허란;김상현 인제대학교 백병원 2011 仁濟醫學 Vol.32 No.-

Acute fatty liver of pregnancy (AFLP) was first described as a specific clinical entity in 1940 and thought to be universally fatal. Maternal mortality in the past approached 75 percent. However early diagnosis and prompt delivery have dramatically improved the prognosis, and maternal mortality rate is now less than 18 percent. A early diagnosis, a prompt delivery, and a intensive supportive care have improved maternal and perinatal outcome. We report a 30—year—old woman at 37 weeks gestation who have multiple complications including acute pancreatitis in AFLP. She was complicated with acute renal failure, disseminated intravascular coagulation, duodenal ulcer bleeding, spontaneous bacterial peritonitis, pulmonary edema and acute pancreatitis.

떫은감 Carotenoid의 색소 안정성

강미정,윤경영,성종환,이광희,김광수 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.4

Studies on extraction and color stability of carotenoids from astringent persimmon(Diospyros kaki) were performed to provide the basic information for the utilization of persimmon pigment as a new source of natural food colorant. The major carotenoids in astringent persimmon were beta-cryptoxanthin, lycopene and beta-carotene. Beta-cryptoxanthin was the first major pigment and lycopene the next. Total carotenoid content in persimmon calculated as beta-carotene equivalent was 107.4 ㎍/g. The data indicated that the astringent persimmon was a good source of carotenoid pigment. The physical and chemical stabilities of carotenoid from astringent persimmon were investigated at various conditions of temperature, pH and antioxidant. The effects of oxygen and light on the stability of carotenoid pigment has been investigated. The factors that cause the discoloration were visible light, temperature above 60, pH below 5 and oxygen. Especially, the carotenoid pigment was very sensitive to light and oxygen. Carotenoid stability was much improved with increasing nitrogen concentration in the atmosphere. Persimmon pigments have been found to be most stable at 5~10. And tocopherol was the most effective inhibitor of the pigment discoloration.

가중치 연관규칙 탐사 알고리즘을 이용한 자동 문헌 분류

강미나;박미영;승현우 서울여자대학교 컴퓨터과학연구소 2004 정보기술논문지 Vol.2 No.-

본 논문에서는 연관규칙 탐사 알고리즘을 이용하여 전문 분야에 대한 문헌을 자동적으로 분류하기 위한 새로운 방법을 제시하였다. 이것은 지식탐사 시스템에 적용되어 대량의 지식 정보를 효율적으로 검색하고 관리하는데 있어 핵심적인 역할을 한다. 자동 문헌 분류는 학습용 문헌에서 전처리 과정과 연관규칙 탐사 알고리즘을 적용하여 분야별 대표 색인어 추출하는 과정과 추출된 각 색인어에 대한 가중치 배열을 적용하여 실험문헌에서 추출한 전공용어와의 유사도를 계산하여 문헌간의 연관성에 따라 자동으로 문헌을 분류하는 과정으로 이루어진다. 마지막으로 제시한 유사도와 기존 유사 계수들에 의한 자동 분류 결과를 비교하여 제한한 방법의 효율성을 입증하였다. In this paper, we proposed a new method of the automatic document classification in professional fields by using the association rule algorithm. This method can be applied to knowledge discovery system and may perform an important role to search and manage a large amount of information efficiently. An automatic document classification is composed of representative keyword sets extraction course by preprocessing procedure and applying association rule algorithm and automatic document classification course by calculating the degree of similarity of major keyword extracted from the testing documents by applying weight array about abstracted each keyword. Finally we compared automatic classification result degree of similarity proposed in this paper with that of the existing similarily coefficients, and show the efficiency of the proposed method.