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푸마르산으로부터 숙신산 생물전환을 위한 Enterococcus faecalis RKY1 균체의 포괄담체에 의한 고정화
위영중,조영심,류화원 전남대학교 촉매연구소 2000 觸媒硏究 論文集 Vol.21 No.-
The Enterococcus faecalis RKY1 cells were immobilized within the polymer matrices such as agar, Ca-alginate, and k -carrageenan in order to investigate bioconversion characteristics of immobilized Enterococcus faecalis RKY1. Agar and k-carrageenan gels were prepared for cubic type, and Ca-alginate gels for bead type. When the seed volume of immobilized cells was adjusted to 10%(v/v), the optimum concentrations of agar, sodium alginate, and k -carrageenan for immo- bilization of Enterococcus faecalis RKY1 cells were 2%(w/v) for all. In this condition, the conversion ratios were 93.6, 96.4, 76.5%, respectively. Immobilized cells entrapped in agar, Ca-alginate, and k -carrageenan have all the optimum pH of 7.0. The optimum temperature of immobilized cells was 40℃ for agar gels, 35℃ for Ca-alginate gels, and 40℃ for k -carrageenan gels. Therefore, Ca-alginate gels were suitable for immobilization of Enterococcus faecalis RKY1 for succinate production.
뇌경색 환자에서의 혈장 지질과 항산화 영양소 상태에 관한 연구
최영선,이난희,서정규,박의현,조성희 경북대학교 병원 1998 경북대학교병원의학연구소논문집 Vol.2 No.1
뇌경색 환자의 생활 습관, 혈장 지질 및 적혈구 지질 및 적혈구 지질의 지방산 조성과 혈장 지질과산화물 농도, 항산화영양소인 비타민 E, A, β-carotene 상태를 측정하여 대조군의 자료화 비교함으로써 뇌경색 환자의 혈장 지질과 항산화영양소 상태를 조사하였다. 18명의 남자 환자군의 평균연령은 65.7(45~76)세였으며 36명의 건강한 남자대조군의 평균연령은 59.2(55~65)세였으며, 체중, 신장 및 BMI는 대조군과 환자군 간에 유의한 차이가 없었다. 환자군의 발병전 흡연 정도와 음주정도는 대조군보다 유의하게 높았으며, 적혈구지방산 조성에 있어서 포화지방산의 비가 유의하게 높았고 다불포화지방산의 비는 유의하게 낮았으며, 통계적인 유의성은 없으나 항산화 영양소 상태가 대조군에 비하여 불량하고 혈장 과산화지질의 종도가 높은 경향을 보였다. 이의 원인으로서는 환자군이 대조군에 비해 흡연율이 높고 음주량이 많으며 운동부족 등 부적합한 생활습관이 주요한 요인으로 작용한 것으로 판단되며, 따라서 바람직한 생활습관이 뇌경색질환의 예방에 기본요건이 된다고 사료된다. To investigate antioxidant and lipid staus of patients with cerebral infarction, 18 male patients admitted to Kyungpook National University Hospital from March to October, 1995 were compared to 36 healty male control subjects. The percentages of heavy smoking, heavy drinking and nonexercise before diagnosis of the disease in the patient group were significantly higher compared to the current status of the control group. Total cholesterol, LDL cholesterol and lipoprotein(a) were significantly higher in the patients than the control subjects. For the fatty acid composition of erythrocytes, total saturated fatty acids(% by weight) were significantly higher and total polyunsaturated fatty acids lower in the patient group than in the control group. Plasma levels of retinol, α-tocopherol and β-carotene were not significantly different between the patient group and the control group at the level of P<0.05 Alpha-tocopherol/total cholesterol of plasma was lower in the patient group with the statistical significance of P=0.054, and the plasma level of TBARS of the patient group was 1.98 nmol MDA/mL as compared to the level of the control, 1.60 nmol MDA/mL(P=0.066). Our results indicate that oxidative stress was increased in the patients with cerebral infraction, and such outcome might have resulted from undesirable life styles.
Isolation and Characterization of a Novel Lactic Acid Bacterium for the Production of Lactic Acid
Wee, Young-Jung,Yun, Jong-Sun,Park, Don-Hee,Ryu, Hwa-Won The Korean Society for Biotechnology and Bioengine 2004 Biotechnology and Bioprocess Engineering Vol.9 No.4
We isolated a novel lactic acid bacterium from a Korean traditional fermented food, soybean paste. The newly isolated strain, dubbed RKY2, grew well on glucose, sucrose, galactose, and fructose, but it could not utilize xylose, starch, or glycerol. When the partially amplified 16S rDNA sequence (772 bp) of the strain RKY2 was compared with 10 reference strains, it was found to be most similar to Lactobacillus pentosus JCM $1588^T$, with 99.74% similarity. There-fore, the strain RKY2 was renamed Lactobacillus sp. RKY2, which has been deposited in the Korean Collection for Type Cultures as KCTC 10353BP. Lactobacillus sp. RKY2 was found to be a homofermentative lactic acid bacterium, because its end-product from glucose metabolism was found to be mainly lactic acid. It could produce more than 90 g/L of lactic acid from MRS medium supplemented with 100 g/L of glucose, with 5.2 g $L^-1$ $h^-1$ of productivity and 0.95 g/g of lactic acid yield.
Jung-Wee Park,Hong Seok Kim,Sojeong Park,Sung Hwa Kim,Young-Kyun Lee,Kyung-Hoi Koo 대한정형외과학회 2022 Clinics in Orthopedic Surgery Vol.14 No.4
Background: Osteonecrosis of the femoral head (ONFH) involves young or middle-aged adults, and its incidence is increasing along with increasing use of steroids in the management of organ transplantation and adjuvant therapy for malignant neoplasms. To date, no pharmacological agent has been proven to prevent or retard the progression of ONFH, and surgical procedures including joint preservation procedures and hip arthroplasties are main treatments for the disease. Although ONFH is the most common or second most common disease for hip arthroplasty in East Asian countries, the trend of surgical procedures in this region remains unknown. Thus, we evaluated trends in surgical treatment of the disease in South Korea. Methods: We identified patients with ONFH from the Korean Health Insurance Review and Assessment (HIRA) database, a nationwide medical claims database of South Korea, between January 2007 and December 2018 and calculated the proportions of following surgical procedures at each year: total hip arthroplasty (THA), hemiarthroplasty (HA), core decompression/multiple drilling, femoral osteotomy, and vascularized bone grafting. Results: The total number of procedures increased from 3,824 in 2007 to 6,929 in 2018. Overall, the rate of THA (86%) was far greater than other procedures. From 2007 to 2018, the percentage of THA among the procedures increased from 80% to 91%, while that of joint preservation procedures decreased from 11% to 5%. Conclusions: The total number of surgical procedures performed for ONFH increased and the percentage of THA increased, while that of joint preservation procedures decreased from 2007 to 2018 in South Korea.
Jung Duk-Yeon,Jung Sunok,Yun Jong-Sun,Kim Jin-Nam,Wee Young-Jung,Jang Hong-Gi,Ryu Hwa-Won The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.4
In this study, the cultural medium used for the efficient production of $\gamma$-PGA with a newly isolated Bacillus sp. RKY3 was optimized. It was necessary to supplement the culture medium with L-glutamic acid and an additional carbon source in order to induce the effective production of $\gamma$-PGA. The amount of $\gamma$-PGA increased with the addition of L-glutamic acid to the medium. The addition of 90 g/L L-glutamic acid to the medium resulted in the maximal yield of $\gamma$-PGA (83.2 g/L). The optimum nitrogen source was determined to be peptone, but corn steep liquor, a cheap nutrient, was also found to be effective for $\gamma$-PGA production. Both the $\gamma$-PGA production and cell growth increased rapidly with the addition of small amounts of $K_2HPO_4$ and $MgSO_4\cdot7H_{2}O$. Bacillus sp. RKY3 appears to require $Mg^{2+}$, rather than $Mn^{2+}$, for $\gamma$-PGA production, which is distinct from the production protocols associated with other, previously reported bacteria. Bacillus sp. RKY3 may also have contributed some minor $\gamma$-PGA depolymerase activity, resulting in the reduction of the molecular weight of the produced $\gamma$-PGA at the end of fermentation.
Wee Young-Jung,Kim Jin-Nam,Yun Jong-Sun,Ryu Hwa-Won The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.1
Lactic acid is a green chemical that can be used as a raw material for biodegradable polymer. To produce lactic acid through microbial fermentation, we previously screened a novel lactic acid bacterium. In this work, we optimized lactic acid fermentation using a newly isolated and homofermentative lactic acid bacterium. The optimum medium components were found to be glucose, yeast extract, $(NH_4)_{2}HPO_4,\;and\;MnSO_4$. The optimum pH and temperature for a batch culture of Lactobacillus sp. RKY2 was found to be 6.0 and $36^{\circ}C$, respectively. Under the optimized culture conditions, the maximum lactic acid concentration (153.9 g/L) was obtained from 200 g/L of glucose and 15 g/L of yeast extract, and maximum lactic acid productivity ($6.21\;gL^{-1}h^{-1}$) was obtained from 100 g/L of glucose and 20 g/L of yeast extract. In all cases, the lactic acid yields were found to be above 0.91 g/g. This article provides the optimized conditions for a batch culture of Lactobacillus sp. RKY2, which resulted in highest productivity of lactic acid.