Hypoxia-Responsive MicroRNA-101 Promotes Angiogenesis <i>via</i> Heme Oxygenase-1/Vascular Endothelial Growth Factor Axis by Targeting Cullin 3

Kim, Ji-Hee,Lee, Kwang-Soon,Lee, Dong-Keon,Kim, Joohwan,Kwak, Su-Nam,Ha, Kwon-Soo,Choe, Jongseon,Won, Moo-Ho,Cho, Byung-Ryul,Jeoung, Dooil,Lee, Hansoo,Kwon, Young-Guen,Kim, Young-Myeong Mary Ann Liebert 2014 Antioxidants & redox signaling Vol.21 No.18

<P>Aims: Hypoxia induces expression of various genes and microRNAs (miRs) that regulate angiogenesis and vascular function. In this study, we investigated a new functional role of new hypoxia-responsive miR-101 in angiogenesis and its underlying mechanism for regulating heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) expression. Results: We found that hypoxia induced miR-101, which binds to the 3 ' untranslated region of cullin 3 (Cul3) and stabilizes nuclear factor erythroid-derived 2-related factor 2 (Nrf2) via inhibition of the proteasomal degradation pathway. miR-101 overexpression promoted Nrf2 nuclear accumulation, which was accompanied with increases in HO-1 induction, VEGF expression, and endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) production. The elevated NO-induced S-nitrosylation of Kelch-like ECH-associated protein 1 and subsequent induction of Nrf2-dependent HO-1 lead to further elevation of VEGF production via a positive feedback loop between the Nrf2/HO-1 and VEGF/eNOS axes. Moreover, miR-101 promoted angiogenic signals and angiogenesis both in vitro and in vivo, and these events were attenuated by inhibiting the biological activity of HO-1, VEGF, or eNOS. Moreover, these effects were also observed in aortic rings from HO-1(+/-) and eNOS(-/-) mice. Local overexpression of miR-101 improved therapeutic angiogenesis and perfusion recovery in the ischemic mouse hindlimb, whereas antagomiR-101 diminished regional blood flow. Innovation: Hypoxia-responsive miR-101 stimulates angiogenesis by activating the HO-1/VEGF/eNOS axis via Cul3 targeting. Thus, miR-101 is a novel angiomir. Conclusion: Our results provide new mechanistic insights into a functional role of miR-101 as a potential therapeutic target in angiogenesis and vascular remodeling. Antioxid. Redox Signal. 21, 2469-2482.</P>

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

中心壓軸力을 받는 高强度 鐵筋콘크리트 기둥의 耐力과 延性에 關한 실험적 硏究

곽노현,이영호,김남주,이경종,정헌수 중앙대학교 기술과학연구소 1997 기술과학연구소 논문집 Vol.27 No.-

A study on the behavior of short reinforced concrete columns was experimently described. Twenty-four concrete columns, with 20cm square and 60cm height, containing 8 longitudinal steel bars and different the amount of ties, spacing and concrete strengths, were subjected to concentric load by failure. Test results indicated that 1) It is incompatible in high-strength concrete to arrange stirrups to the AIK code of D/2 throughout the example of 10cm stirrup spacing following AIK codes. 2) As the amount of stirrup increased, the strength and ductily of reinforced concrete column improved. 3) As the concrete strength is high, the effect of the was reduced. 4) If strength enhancement factors increased, strain at maximum load also does and ductility ratios does almost linearliary. 5) The rate of strength enhancement factor does well with the modified kent & Park's theory. 6) As to the result of calculating the effective confined area which is suggested in Sheikh & Uzumeri's theory, the middle vertical steel is effective, only if the stirrups are arranged with spacing of 3cm.

객체 지향모델인 Actor에서 최대 병렬성 지원의 순차 합성법

곽호영,김장형,안기중 제주대학교 1991 논문집 Vol.33 No.-

Actor 모델은 병렬 처리 구조를 기반으로 하는 병행 합성 방법을 사용하고 있기 때문에 특별히 문장의 처리 순서가 유지되어야 하는 순차 합성의 경우에는 고객 actor를 생성하여 이용하는 방법으로 처리하고 있다. 그러나, 이 고객 actor로 하여금 다음 문장의 처리를 전가시키는 방법에서 발생되는 병렬성 감소로 인하여 Actor 모델 자체의 미세화 병행성 개념에 위배된다. 따라서, 본 논문에서는 이를 개선하기 위한 방법으로서 순차 합성의 경우에 각 프로세스들 간에 의존성을 평가하여 동일한 시스템을 유지하면서 병렬성을 극대화하는 MPS 알고리즘을 세 가지로 제시하였다. 제안된 알고리즘을 이용한 MPS는 앞장에서 살펴본 바와 같이 그 병렬성은 기존의 Actor 모델에서와 같이 고객 actor를 이용하는 처리 방식보다 증가되었으며, 또한 [정리 1,2]를 이용한 알고리즘보다는 전달 기능의 고객 actor를 제거하는 방법이 궁극적으로 프로세스의 수를 줄일 수 있기 때문에 매우 효율적인 방법으로 평가되었다. 이 알고리즘은 Actor 모델의 구현을 위한 커널의 설계에 있어서 병렬 처리 구조가 갖는 병행 처리의 정도를 최대로 부여하기 위해 제안하였다. In Actor model based on message-passing mechanism and parallel architecture, all commands in an Actor are processed concurrently. However, when sequential processing of commands must be preserved, it can be solved by creating actors called customers which are to do delegation, because concurrent compositions inherent in Actor, are not enough to do the sequential processing of commands. In this paper, the dependency between expressions in the sequential composition of actor commands is analyzed, and the sequential composition method for supporting minimal sequentiality and maximal parallelism in the Actor model is presented.

D-Amino Acid Aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구

곽미선,이승구,정상철,서승현,이재흥,전영중,김영호,성문희 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acids, 1,300 thermophiles isolated from the soil of Korea were analyzed for D-amino acid aminotransferase (D-AAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two therrnophiles, Bacillus sp. LK-I and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-0l, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.

컴파일러 최적화를 위한 코드 스케쥴링

곽호영,한정현 제주대학교 1992 논문집 Vol.35 No.-

This paper deals with instruction scheduling, one area of compiler optimization. This assures the correctness of program execution during occuring the interlock and reduces the execution time in pipelined processor. DAG(Directed Acyclic Graph) is used to represent the problem and is proven to be pretty convinient to represent the various Kinds of serialization constraints. Gibbon's scheduling algorithm with O(n²) is introdued to schedule the instruction from DAG. Three heuristics and some assumptions are explained to prevent the problem to be O(n⁴). Furthermore, the impact on another phase in compiler is discused. Actually this scheduling algorithm assumes to perform the instruction scheduling after register allocation. More work relating to this area will be the relationship beween two phases-register allocation and code schduling.

관상 동맥 질환에서 아포 E 지단백 유전자 다형성과 혈청 지질치와의 관계

곽선영,김성구,정호석,이유경,이광희,김철현,최태명,현민수,권영주 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background and aims: The Apolipoprotein E is a ligand of both the protein component LDL receptor as well as the apo E LDL-Receptor related protein (LRP). It modulates the receptor binding of lipoproteins, with the apolipoprotein E found on cell surfaces as its component, thus serving an important role in the lipid metabolism by carrying out the intracellular transport of cholesterol in lipoproteins. The gene for apolipoprotein E is the product of three common genotypes as well as many more rare alleles. The common genotypes are ε2, ε3, and ε4, and are expressed in the three phenotype isoforms of E2, E3, and E4. In the event that E4 is the main component, a rise in the cholesterol level, as the result of down-regulation of the LDL receptor, is observed. Therefore, those samples with E4 genotypes are known to be in much higher risk of coronary artery disease than those with ε3/ε3, while those with ε2 are in low risk (with the exception of hypertiglyceremai Ⅲ). The aim of this study is to analyze in patients with ischemic heart disease the role of aplipoprotein E alleles in order to seek its correlation with coronary artery disease, as well as to seek whether the polymorphism of apo E produces any differences in the severity of coronary artery disease according to plasma lipid levels. Methods: The subjects for study were 273 patients admitted to the Internal Cardiology Division of the Soonchunhyang University Hospital form December 1998 to February 1999. The subjects were divided into the two groups of which one was ischemic heart disease (IHD) experiment group totaling 105 (avg.60.1 years of age, male/female ratio = 69/36) and the control group totaling 168 (avg. 59.7 years of age, male/female = 73:95). The coronary angiogram was given to 127 subjects, and of this total, 94 have developed significant stenosis in the coronary artery. The stages of the analyzing of the apo E phenotype was first, the separation of DNA from the blood samples, subjecting it to the PCR from with 228 base pairs of expanded products were obtained. The band was determined by means of the reverse hybridization principle on the nitrocellulose strip. Results: From the 105 patients the distributions of apo E phenotypes were as follows: ε3/2(5.7%), ε4/2(1.9%), ε3/3(70%), ε4/3(20%), ε4/4(1.9%). The relative frequencies of each allele are as follow: ε2 (0.038), ε3 (0.833), ε4 (0.128). The results show as follows: ⅰ) The IHD experiment group to have a higher occurrence of ε4/3 phenotypes as well as ε4 alleles than the control group. ⅱ) Both the control group and IHD group showed the largest distribution of ε3/3 for phenotypes, and ε4 for alleles. ⅲ) The IHD group showed less ε2/3 phenotypes as well as significantly less allele frequency of ε3 in comparison to the control group. ⅳ) the IHD group showed a much lower level of HDL in comparison to the control group, while the LDL was significantly higher; samples including the apo ε2 showed a significantly higher level of HDL than those without. Among the control group, samples including apo ε2 showed a significantly higher level of TG (triglyceride) than samples without. No significant difference was found between the experiment apo ε4 sample and the control plasma lipid sample. ⅴ) No significant correlation was found between an apo E polynorphism and the number of involved arteries of a coronary angiogram. Conclusion: Between the experiment IHD group and control group were found differences in the frequency of alleles. The polymorphism of apo E alleles may contribute as a risk factor to the development of heart disease by involving itself in the metabolism and modulation of plasma lipids.

全北地方의 藥用作物 재배 및 生産現況

郭晙洙,金昌洙,宋英恩,金柱,朴珍華,韓宗鉉,宋昊俊 圓光大學校 韓醫學硏究所 1997 원광한의학 Vol.7 No.1

본격적인 지방화 시대를 맞이하면서 지역특화작물의 개발과 농가소득증대를 위한 새로운 기술개발이 절실한 이때 주요 소득원작물의 하나인 약용작물의 재배 및 생산현황을 파악하여 지역특성에 맞는 새로운 작목개발 및 정책 입안 자료로 활용하고자 조사분석한 결과는 다음과 같다. 1. 생산량을 기준으로한 전북지방의 주요 약용작물을 특화계수 분석결과 총 31개작목 중 독활, 백지, 양유, 오미자, 지황 등은 핵심품목으로 길경, 두층, 방풍, 작약, 천마 등은 주산품목으로 조사되었으며 강활 등 21개 작목은 비특화 작물로 분석되었다. 재배면적을 기준으로 한결과는 독활, 백지, 양유, 지황 등이 핵심품목으로 길경, 두층, 목단, 방풍, 백출, 오미자, 의이인, 작약 등은 주산품목으로 강활 등 19개 품목은 비특화 작물로 분석되었다. 2. 주요 약용작물 재배 계획면적 조사에서는 당귀, 두층, 오미자, 독활, 천궁 등이 계획 대비 실제 재배면적의 차이가 큰 작목으로서 이들은 일반적으로 상품가격의 영향을 많이 받는 것으로 분석되었다. 3. '96 재배면적 조사 결과 전북지방의 주요 약용작물 재배 계획 면적은 총 1,692.7ha였으며 이중 독활이 426ha로 가장 많았고, 길경(283ha), 양유(179ha), 작약(165ha), 두층(154ha), 당귀(113ha), 오미자 의이인, 지황, 산수유, 천궁, 방풍 등의 순이었으며 기타작목으로 백지, 황기, 백하수오 등 28작목이 조사되었다. With the beginning of the new localization era, epoch-making techniques in local agriculture are needed to improve farm income and devlop a locally specialized crops. On the basis of these trends in local agriculture, the status quo of production in medicinal plants which are one of the major income crops were surveyed and analyzed the data for developing a new crops and drafting a policy. The major results are as follows; 1. The results analyzed by Location Quotient of production rate in Chonbuk were classified three catagories. 5 items including Aralia continentalis K_ITAGAWA among 31 items were so rted out as a core crops, another 5 items including Platycodon grandiflotum(J_ACQ.) A.DC. were as a main crops and other 21 items were as a non-specialized crops. Analyzed by cultivation area, 4 items were sorted out as a core crops, another 8 items were as a main crops and the other 19 items were as a non-specialized crops. 2. In comparison with the gap between intended cultivation area and real cultivation area, 5 items including Eucommia ulmoides OLIVER were showed more larger than the others. The above mentioned items were assumed to be more influenced by the price. 3. Farmer's intended cultivation areas of the major medidinal plants in 1996 were surveyed totals of 1.692 ha in Chonbuk. Among them, Aralia corolata was surveyed as the top totals of 426 ha. in area.

황금(Scutellaria baicalensis) flavonoid의 대량 분리

곽호영,김동현,이윤형,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

황금의 뿌리를 물 또는 80% MeOH 로 추출하고 각각 농축하였다. MeOH 추출물은 물, EtOAc 및 n-BuOH로 분배 추출하였다. 얻어진 분획물에 대하여 silica gel column chromatography 하거나, 침전 및 여과과정을 반복하여 4종의 flavonoids 즉, baicalin, baicalein, wogonin 및 wogonoside를 높은 수율로 분리하였다. 분리한 화합물의 화학구조는 NMR을 포함한 물리화학적 data를 해석하여 동정하였다. The root of Scutellaria baicalensis was extracted with water or 80% aqueous MeOH and concentrated, respectively. The MeOH extract was partitioned between H_(2)O with organic solvents such as EtOAc and n-BuOH. The silica gel column chromatography or the precipitation method led to the isolation of four pure flavonoids, baicalin, baicalein, wogonin and wogonosides, with very high yield. The identification of the obtained flavonoids was carried out from the result of physico-chemical data including NMR.

pH 5.5에서 재광화 용액의 포화도 변화가 인공 탈회된 법랑질의 재광화에 미치는 영향

곽영준,김의성,박성호,공형규,이윤,이찬영 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.5

초기 법랑질 우식의 경우 주위의 pH. 유기산의 종류 및 농도,치아의 무기질 및 유기질의 화학적 조성,불소의 농도,용액의 포화도등에 의해 영향을 받는다고 하였다. 본 연구에서는 lactic acid를 이용하여 법랑질을 인공 탈회시키고, pH 5.5인 포화도가 다른 세 가지 재광화 용액 (포화도 0.25,0.30, 0.35)으로 12일간 처리한 후,편광 현미경에서 얻은 상에서 탈회 깊이의 변화,우식 표면층 폭의 변화,무기질의 양적 변화를 Photoshop, Image pro plus, Scion Image, Excel을 이용하여 비교,분석하였다. 1. pH 5.5 에서 재광화 용액의 포화도가 증가할수록 우식 표면층의 폭은 증가하였다. 2. pH 5.5 에서 l 군의 경우 우식 부위에 전반적으로 재광화가 일어났으나,3군의 경우 우식 표면층에서만 재광화의 양상이 뚜렷하게 나타났고, 2군에서는 1군과 3군의 중간적인 양상이 나타났다. 한편,3군의 경우 1, 2 군보다 우식 표면층의 폭이 유의하게 증가하였다. 결론적으로 본 실험의 조건에서 포화도가 가장 낮았던 군에서는 우식 표면층에서 우식 섬부까지 전반적으로 재광화가 일어난 반면,포화도가 높아질수록 우식 표면층의 폭은 증가하였으나 우식 심부에서는 큰 변화를 보이지 않는 양상이 나타났다. The purpose of this study is to observe and compare the remineralization tendencies of artificially demineralized enamel by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel). For this study, 36 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 3 days in order to produce dental caries artificially that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffered remineralization solution of three different degrees of saturation (0.25, 0.30, 0.35) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (× 100). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were determined. In conclusion, in the group with the lowest degree of saturation, remineralization occurred thoroughly from the surface to the subsurface lesion, whereas in the groups with greater degree of saturation showed no significant change in the subsurface lesion, although there was corresponding increase in the remineralization width on the surface zones.