영골(靈骨).강압혈(降壓穴) 자극(刺鍼)이 혈압(血壓)에 미치는 영향(影向)

임영남,심성용,한지완,고호연,박종형,한양희,전찬용,김동우,Yim, Young-Nam,Sim, Sung-Yong,Han, Ji-Wan,Ko, Ho-Yeon,Park, Chong-Hyeong,Han, Yang-Hee,Chun, Chan-Yong,Kim, Dong-Woo 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.2

Objective : The purpose of this study is to evaluate the clinical effect of the non-pharmacological anti-hypertension method, acupuncture, for hypertensive patients. Methods : 26 patients diagnosed with hypertension were each treated by one of three methods: an anti-hypertension drug(adalat soft capsule), venepuncture, and acupuncture. In cases of rising blood pressure, patients were treated by their one of the three methods, and blood pressure was measured at regular intervals. Result : As for the group of acupuncture treated patients on Young-gol(ling-gu) and Gang-ap-hyul(jiang-ya-xue), readings dropped 18.0mmHg in systolic blood pressure and 6.50mmHg in diastolic blood pressure. This was a greater effect than that seen in the venepuncture treated patients. Blood pressure checked one hour after acupuncture treatment showed a greater difference than blood pressure checked after thirty minutes. Conclusion : Safe reductions in blood pressure were observed through the non-pharmacological method of acupuncture on Young-gol(ling-gu) and Gang-ap-hyul(Jiang-ya-xue).

S-2 (3-aminopropylamino)ethylphosphorothioic acid (WR-2721)가 방사선에 조사된 흰쥐의 효소 활성에 미치는 영향

고성진,이천복,김재영 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1997 Journal of biomedical laboratory sciences Vol.3 No.1

S-2 (3-aminopropylamino)ethylphosphorothioic acid (WR-2721)이 방사선에 대한 방어효과에 미치는 영향을 규명하고자 Sprague-Dawley계 웅성 흰쥐를 대조군, WR-2721 단독투여군 (200㎎/㎏), X-선 단독조사군, WR-2721투여 (200㎎/㎏)후 X-선 조사한 병용군으로 나누어 X-선 8 Gy선량을 전신조사한 후 1,3,7,10일 간에 각각 혈액을 채취하여 효소활성치와 glucose함량 변화를 측정하여 다음과 같은 결과를 얻었다. X-선 단독조사군에서는 ALP와 AST의 활성치가 대조군에 비하여 감소하였으나 WR-2721 을 병용한 군에서는 그 감소폭이 줄어들었고, ALT와 LDH의 활성치는 X-선 단독조사군에서 대조군에 비하여 증가하였고, WR-2721을 병용한 군에서는 그 증가폭이 감소되었다. 또한 glucose치의 변동은 X-선 단독조사군에서 대조군에 비하여 유의성 있게 증가하였으나 WR-2721을 병용한 군에서는 그 증가폭이 감소되었다. 이로 미루어 보아 WR-2721이 X-선으로부터 생체내 주요 장기들을 보호하는 작용이 있음을 시사하고 있다. Male rats of Albino strain were divided into four groups, control group, X-irradiated group, WR-2721 treatment group and X-irradiated group treated with WR-2721. The radioprotective effect of treatment with S-2 (3-aminopropylamino)ethylphosphorothioic acid (WR-2721) in the dose of 200㎎/㎏ by intraperitonial injection on rats 20min prior to wholebody X-irradiation (8Gy) was studied. Each group determined serum alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) activities and contents of serum glucose after 1, 3, 7 and 10 days. The ALP and AST activities of X-irradiated group were significantly decreased (p<0.05) compared with that of control group, but X-irradated group treated with WR-2721 less decreased those enzyme activities compared with the X-irradiated group. X-irradiated group was significantly increased (p<0.05) ALT and LDH activities compared with that of control group, but X-irradated group treated with WR-2721 less increased those enzyme activities compared with the X-irradiated group. The concentration of serum glucose of X-irradiated group was signficantly increased (p<0.05) compared with that of control group, but X-irradated group treated with WR-2721 less increased compared with that of X-irradiated group. It may be considered that WR-2721 provided radioprotective effect of organs of body from X-irradiation.

疎水性 環境이 Spermine에 의하여 誘發되는 凝縮된 DNA의 異例的 吸光度-溫度樣相에 미치는 影響

高東成,李天培,崔宇永,李璨容 충남대학교 1985 忠南科學硏究誌 Vol.12 No.2

We have investigated the effect of hydrophobic environmental reagents (urea and DMSO) on the anomalous absorbance-temperature profile of condensed DNA, induced by spermine. The cooperativity of the phase transition to the trough region in the anomalous absorbance-temperature profile is more sensitive to the hydrophobic environmental reagents than that of the melting region, and the trough peak size can be decreased by increased concentration of the hydrophobic reagents. The present data indicate the importance of hydrophobic interaction in the stabilization of the condensed state of DNA induced by spermine, and that the anomalous absorbance-temperature profile is due to the thermal stabilization of the tertiary structure of the spermine-induced collapsed state of the DNA.

疎水性 環境이 Spermine에 의하여 誘發되는 凝縮된 DNA의 異例的 吸光度 : 溫度樣相에 미치는 影響 Temperature Profile of Condensed DNA, Induced by Spermine

高東成,李天培,崔宇永,李璨容 충남대학교 기초과학연구소 1986 연구논문집 Vol.6 No.-

We have investigated the effect of hydrophobic environmental reagents (urea and DMSO) on the anomalous absorbance-temperature profile of condensed DNA, induced by spermine. The cooperativity of the phase transition to the trough region in the anomalous absorbance-temperature profile is more sensitive to the hydrophobic environmental reagents than that of the melting region, and the trough peak size can be decreased by increased concentration of the hydrophobic reagents. The present data indicate the importance of hydrophobic interaction in the stabilization of the condensed state of DNA induced by spermine, and that the anomalous absorbance-temperature profile is due to the thermal stabilization of the tertiary structure of the spermine-induced collapsed state of the DNA.

Electrochemical Studies of Co(II) Complexes Containing Tetradentate Schiff-Base Ligands

고영춘,정병구 대불대학교 1999 論文集 Vol.5 No.1

A series of tetradentante Schiff-base ligands ; 1,3-bis(salicylideneimino)propane [SALPRH2], 1,4-bis(salicylideneimino)butane [SALBUH2], and 1,5-bis(salicylideneimino)pentane[SALPEH2], and their Co(II) complexes were synthesized. The properties of ligands and Co(II) complexes were characterized by elemental analysis, IR, NMR, and UV-Vis spectra, molar conductance, and thermogravimetric analysis. The mole ratio of Schiff base to cobalt at complexes was found to be 1:1 All complexes were four-coordinated configuration, [Co(II)(L)](L is dianionic ligand) and non-ionic compound. The redox process of the ligands and their Co(II) complexes in DMSO solution containing 0.1 M TEAP as supporting electrolyte was investigated by cyclic voltammetry, chronoamperometry, and differential pulse voltammetry at glassy carbon electrode and by controlled potential coulometry at platinum gauze electrode. The redox process of the ligands was highly irreversible, whereas redox process of Co(II) complexes was observed as one electron transfer process of quasi-reversible and diffusion-controlled reaction. Also the electrochemical redox potentials of the Co(II) complexes were affected by chelate ring size of ligands. The diffusion coefficients of Co(II) complexes in DMSO solution were determined to be 5.7∼8.3 x 10-6 ㎠/sec. Also, the exchange rate constants were determined to be 7.8∼8.7 x 10-2 cm/sec.

염화나트륨 수용액에서 비스(1,10-페난트롤린)구리(II) : 도데실황산나트륨 용액의 전기화학적 거동

고영춘 대불대학교 1998 論文集 Vol.4 No.1

Electerochemical behaviors of 1,0 mM bis(1,10-phenanthroline)copper(Ⅱ)(Cu(ph)_(2)^(2+)) in 100 mM NaCl solution, without and with sodium dodecyl sulfate(SDS), were investigated. In the presence of SDS, E_(pa) and E_(1/2) of Cu(ph)_(2)^(2+/+) shift to the negative direction compared to the SDS free. The intersection of two lines on ΔE_(p) vs. -log[SDS] was determined as a critial micelle concentraction (CMC).

스페르민-DNA 복합체의 이례적 흡광도-온도 양상에 미치는 DMSO의 영향

고동성,이천배,최우영,이찬용 충남대학교 기초과학연구소 1986 연구논문집 Vol.6 No.-

DNA의 응축을 유발시키는 spermine 농도 존재시 downward peak(trough)를 갖는 이례적 흡광도-온도 양상에 hydrophobic environment를 조성하는 DMSO의 영향을 조사하였다. DMSO 농도증가에 따라 downward peak의 깊이가 점차 얕아졌으며 그 phase transition enthalpy와 relative cooperative length(n)가 melting region보다는 downward peak(trough) phase transition에 더 예민하게 영향을 받았다. 따라서 본 data는 spermine에 의하여 유발되는 응축된 DNA 구조에 이르는 hydrophobic interaction에 의한 tertiary structure 형성이 온도상승에 따라 유리해짐으로써 downward peak가 형성된다는 추리를 확인하는 실험결과가 될 것이다. In an effort to improve our knowledge on the mechanism of the collapse of DNA into a compact structure by polyamines, we investigated the effect of dimethyl sulfoxide (DMSO), a hydrophobic environmental reagent on the anomalous absorbance-temperature profile of condensed DNA, induced by spermine and having a trough preceeded by the initiation of the melting phase. The trough peak size can be decreased by increased concentration of DMSO, and the cooperativity of the phase transition to the region in the anomalous absorbance-temperature profile is more sensitive to DMSO than that of the melting regions of the profile. The present data indicate that the anomalous absorbance-temperatuve profile is due to the thermal stabilization of the tertiary structure of the spermine-induced collapsed state of DNA and the importance of hydrophobic interaction in the stabilization of the condensed state of DNA induced by spermine.

Li-CICs의 Deintercalation에 따른 구조변이와 열적, 전기적 안정성과의 관계(Ⅱ) : The relation of structural transition, thermal and electrical stability deinter calation of Li-CICs(Ⅱ):For Li-EaGDICs and Li-EGDICs

오원춘,박정오,백대진,고영신 한국분석과학회 1996 분석과학 Vol.9 No.1

가온 가압법에 의하여 합성된 Li-EaGICs, Li-EGlCs를 자발적으로 분해시켜서 이들의 deintercalation 과정에 대하여 X-선 회절분석, 열분해분석 몇 전기비저항값을 측정하여 이들 결과에 대하여 토론하였다. Intercalation에 대한 X-선 회절분석 결과에 의하면, Li-EaGlCs와 Li-EGlCs는 1 stage가 완전하게 형성되지 않았고, 주로 저차 stage가 형성되었다. 또한 deintercalation 결과에 의하면, 4주 이후에는 deintercalation이 멈추었으며 Li-EGDICs는 Li-EaGDlCs보다 층간 잔유 금속을 많이 가지고 있음을 알 수 있다. 열분해분석 결과에 의하면 두 가지 화합물 모두 강한 발열반응을 수반하였으며, 또한 400℃ 이 상에서는 반응이 수반되지 않는 것으로 보아 intercalants들이 완전히 deintercalation되었음을 알 수 있다. 전기 비저항 측정 결과에 의하면 Li-EGDICs는 상대적으로 낮은 비저항값을 가지고 있었으며, Li-EaGDICs는 이상적인 비저항 곡선을 나타내었다. 이들 결과로부터 Li-EaGlCs가 Li-EGICs보다 2차 전지의 양극재료로서 더 좋은 성질을 가지고 있음을 알 수 있다.

C_3 및 C_7위치에 치환된 새로운 세파로스포린 化合物의 合成과 抗菌活性(Ⅰ)

황화영,하재천,김영수,고옥현,강형룡 조선대학교 약학연구소 1994 藥學硏究誌 Vol.16 No.1

The synthesis and antimicrobial activity of cephalosporin having (Z)-2-(2-aminothiazol-4-yl)-2-trityloxyiminoacetamido group and 5-(2-chlorophenyl)-4-phenyl-4H-1, 2, 4-triazol-3-thiomethyl group on the C_3 and C_7 position of the cephem ring, respectively are descrived. This compound was tested for antimicrobial activity in vitro against Candida albicans ATCC 10231, Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 6538P, Mycobacterium phlei IIP-IPH, Escherichia coli KCTC 1039, Escherichia coli ESS, Klebisiella pneumoniae KCTC 1560, Salmonella typhymurium TV-119, Pseudomonas aeruginosa IFO 13130. The antimicrobial activity of synthetic compound was better as compare with cefotaxime and cefazoline against Micrococcus luteus ATCC 9341 & Escherichia coli KCTC ESS.

숯 처리에 의한 녹차 추출액 중 질산태질소 제거 효과

김진우,고영옥,신동국,이 협,전현식,이종옥 진주산업대학교 산업과학기술연구소 2005 산업과학기술연구소보 Vol.- No.12

This experiment was carried out to measure the elimination effect of nitrate nitrogen from green tea extracts through the column system filled with charcoal activated and bamboo charcoal powder compare to that of an nitrate nitrogen standard solution prepared with KNO3 chemicals. The change patterns of pH, and the amount of removed nitrate nitrogen from green tea and nitrate nitrogen standard solution were analyzed according to time course and the amount of charcoal treatments. The results were summarized as follow: 1. pH The pH of Green tea extractions treated with charcoal was high up to 8.17(after 30min treated with 0.4% charcoal) and 8.36(after 60min with 0.4% charcoal) which pH of non-treated green tea (control) was 5.8 The results indicated that the pH values were variable to the treated amount of charcoal and the teated time course. 2. Nitrate nitrogen From the nitrate nitrogen standard solution, the least rate 69.1% and the highest rate 74.5% of nitrate nitrogen were observed to be absorbed through the column treatment (60 min), which with green tea extract solution 21.3% (the least) and 43.8% (the highest) were detected to be absorbed through column treatment (after 60min with 0.4% charcoal). In the case of bamboo treatments with nitrate nitrogen solution samples were showed the least rate 62.8% (30min with 0.1% bamboo) and the highest rate 68.9% (60min with 0.4% bamboo), and with green tea extract solution were observed the least 13.6 (30min with 0.1% bamboo) the highest rate 29.4% (60min with 0.4% bamboo). In general, the elimination activity of nitrate nitrogen was higher by the charcoal activated powder treatment than bamboo charcoal.