Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

담관결석증이 동반된 담도회충증 1예

주봉덕,유철재,이헌영 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1

Biliary ascariasis results not only in biliary tract disease, but also in secondary stone formation, hemorrhage into biliaty tract, acute cholecystitis, biliary perforation, invasion to pancreatic duct, pancreatitis, he%atitis, and liver abscess. In recent, we experienced a case of biliary ascariasis with concomitant biliary stones, which was identified by ultrasonogram percutaneous transhepatic cholangiography, and computed tomography, thereby reporting it herein with a review of literatures.

1999년에 발생한 기상재해 유형별 벼 수량반응조사 연구

주영철,임갑준,한상욱,박중수,조영철,김순재 한국농림기상학회 2000 한국농림기상학회지 Vol.2 No.1

The objectives of this study were to investigate weather conditions which induced discolored grains and viviparous germination, and to evaluate yield responses following viviparous germination during mid- and late- ripening stage, the submergence during reproductive growth stage, and lodging in the yellow ripe stage. Weather conditions which caused glume discoloration at heading stage were 21.3-26.4℃ in average temperature, 75.2-98.4% in relative humidity, 19.3 in transpiration coefficient and 10.8-13.8 m/sec. in wind speed. Yield reduction was 26-27% and 10∼17%, respectively, when the glume discoloration rates were 63.2-65.7% and 38.3-45.2%, obviously due to the decrease in percent of fertile grain and ripening ratio. Weather conditions during continuous rain for 7 days were 96% in relative humidity, 18.9℃ in average temperature, 21.9℃ in maximum temperature, and 16.8℃ in minimum temperature, causing the most viviparous germination in Juanbyeo(45.5%), followed by Jinbubyeo(14.5%), Bongkwangbyeo(14.2%), and Obongbyeo(12.6%). Lateral tillers started to occur when the submergence at the depth of 1.5-2 m lasted one day during the reproductive growth stage. The submergence for 2-3 days at 3-4 m of water depth induced 269-571 lateral tillers/㎡, supporting 32-52% of the total yield. The rice yield in the paddy fields which were left under the lodging conditions until harvesting was not different compared to that of the paddy fields which were kept upright by tieing them together after lodging, but perfect grain ratio decreased about 9.1% in the transplanting culture and 12.5% in the direct seeding culture on dry paddy field because of the increase in immature grains.

자궁경부 상피내종양의 진단 및 치료에서 환상 투열요법(LLETZ)의 임상적 의의

김승철,이경순,김영주,정혜원,안정자 梨花女子大學校 醫科大學 醫科學硏究所 1994 EMJ (Ewha medical journal) Vol.17 No.4

The technique of large loop excision of the transformation zone(LLETZ) as an alternative to destructive/ablative methods of treatment of CIN(cervical intraepihelial neoplasia) such as cryotherapy, laser vaporization and cone biopsy was assessed. In 63 women with suspected CIN attending the colposcopy clinic, the histologic diagnoses made from excision biopsies taken by LLETZ were compaired with those from colposcopically directed punch biopsies. This microinvasive tumors were found by LLETZ and the diagnosis made by LLETZ was worse than that made by punch biopsy in 17 cases(26.9%). That is to say, LLETZ improves the accuracy of diagnosis of CIN. And in offers a cheap and accurate alternative to laser vaporization or cone biopsy, and also combines the benifits of the gold standard cone biopsy with that of local ablative metholds. LLETZ is considered as a fairly new and good method of treatment of CIN, and the olng-term complications or effects should by yet fully evaluated in future.

활성탄을 이용한 낙동강 상수원수의 수처리 효과

임영성,강관호,이홍재,서동철,허종수,손보균,조주식 한국환경농학회 2002 한국환경농학회지 Vol.21 No.3

상수원수를 보다 효과적으로 처리함으로서 양질의 수돗물을 공급하기 위한 기초자료를 얻고자 낙동강 상수원수를 대상으로 활성탄처리에 의한 공탐체류시간 및 활성탄 여층 깊이에 따른 수처리 효율과 생물활성탄으로서의 이용 가능성을 조사한 결과는 다음과 같다. 공탑체류시간(EBCT)에 따른 수처리 효율은 EBCT가 증가할수록 증가되었으나 운전시간이 경과함에 따라 활성탄 흡착능력은 감소되어 처리효율도 서서히 감소하였다. 활성탄 여층깊이에 따른 pH 변화는 활성탄 층 깊이에 따라 거의 없었으며, D0는 활성탄 층 깊이가 깊을수록 서서히 감소하였다. KMnO^4 소비량, UV254 흡광물질 DOC 및 THMFP 처리효율은 활성만 표층으로부터 하부로 내려갈수록 증가하였으며, 운전시간이 경과할수록 활성탄 상층부에 형성되어 있던 흡착대파 하부로 이동하였다. DOC의 상당 부분이 활성탄여과지에 서식하는 미생물 작용에 의해 분해 제거되는 것으로 나타났으며, 운전개시 126일 후의 BAC에서 활성탄 표층으로 부터깊이 20 cm 부근에 미생물이 1.1*10^7 cell/cm^3 이상 존재하는 것으로 관찰되어 생물활성탄 조건을 만족시키고 있었다. This study was carried out to evaluate the pollutant removal efficiencies of the advanced drinking water treatment using mated carbon process. For raw water, Nakdong river was used. From the activated carbon adsorption experiment, the following results were obtained. The efficiency of water treatment enhanced with increase in empty bed contact time. Variation of pH was not detected to the bed depth, but DO content gradually decreased with the bed depth, Removal efficiency of KMnO₄ consumption, UV254 absorption, DOC and THMFP also were increased by increasing in the bed depth. Transition of adsorption zone from upper parts of the bed to the lower parts were detected as treatment periods increased. Large portion of DOCs were degraded and removed by the microbes growing on the surface of activated carbons. Cell numbers of microbes were estimated over 1.1 × 10 exp(7) cell/㎤ at the depth of 20 ㎝ from the surface 126 days after starting operation. The results shown that the activated carbon filter was successfully acted as a biofilm filter.

저전력의 플립-플롭과 클럭 드라이버 설계

양윤주,하영철,송정근,홍창희 東亞大學校 附設 情報通信硏究所 1999 情報通信硏究所論文誌 Vol.7 No.1

본 논문에서는 VLSI 전체 전력소비에 있어 비중이 높은 클럭 시스템의 전압 스윙 폭을 줄여 전력소비를 감소시킬 수 있는 저전압 클럭용 플립플롭을 제안한다. 제안된 플립플롭이 저전압 스윙 클럭으로도 동작함을 증명하고 이 플립플롭과 연계하기 위한 실제 전압 폭 감소 클럭 드라이버를 설계하였으며, 이 클럭 드라이버에 의해 기존의 클럭 드라이버에서 발생하는 전력소비를 1/4가량 감소시킬 수 있음을 설명했다. 본 논문에서 제시하는 플립플롭과 클럭 드라이버간의 연계동작과 소비전력 감소 효과는 SPLICE simulation을 통해 검증하였다.

PCR을 이용한 Rat 기관지 세척액에서의 Pneumocystis carinii DNA의 검출 : 기초실험 A pilot study

김주옥,홍석철,한표성,이종진,김선영,이영하,신대환 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

Background: Pneumocystis carinii (PC) is a opportunistic pathogen causing serious pneumonia (Pneumocystis carinii pneumonia : PCP) in immunosuppressed patients including AIDS. Laboratory diagnosis of PCP is dependent on microscopic demonstration of the PC by using cytochemical staining or by immunocytochemical staining with monoclonal antibodies. However, these staining methods are not highly sensitive. The development of the polymerase chain reaction (PCR) has made it possible to detect very small numbers of pathogens in clinical specimens. To establish the usefulness of PCR for detection of PC DNA, We performed PCR as a pilot study. Methods: PCP was induced on Spraque-Dawley rats with prednisolone 5mg IM twice a week. Bronchial lavages, impression smears, and permanent sections were performed from the 3rd week to 7th week of cortisonized rats. PC DNA was extracted with bead beater / 10% CTAB method. Results: Impression smears showed PC cyst after the 4th week (100%, 28/28 rats), and permanent sections showed 27/28 rats. Control rats showed some PC cysts (2/7). PCR result was positive only one case among the 6th week rats (1/7), But positive 6 cases at the 7th week rats, (6/7). Among control rats. 2 cases were positive (2/7). ??This data is a pilot study for the PC DNA detection using a PCR. PCP were successfully cortisonized rats. If adequate bronchial washing technique will be used, PCR can be a sensitive for PC DNA detection.

포스트스크립트 이름 오브젝트의 저장 및 수행 방법에 관한 연구

김영상,이태주,이종철 경북대학교 산업기술연구소 1991 産業技術硏究誌 Vol.19 No.-

We tried an approach of insertion and execution technique for the Postscript name object in this paper. An application program generates a Postscript page description of a desired document and transmits it to a Postscript interpreter. A name object is ordinarily created when the scanner encounters a Postscript token consisting entirely of regular characters. Such names can be associated with values in dictionaries. When the Postscript interpreter encounters an executable name, it look up the name in the current dictionary context and interprets whatever it find as a result of that name look up.

광범위 베타 락탐계 항생제 분해 효소를 생성하는 폐렴간균에 의한 균혈증이 발생한 환자에서 감영의 위험 인자 및 치료 결과

강철인,김성한,방지환,김홍빈,박상원,최영주,오명돈,김의종,최강원 대한감염학회 2003 감염과 화학요법 Vol.35 No.2

목적 : 본 연구는 ESBL을 생성하는 K. pneumoniae에 의한 균혈증 환자에서 감염의 위험 인자 및 치료 결과를 알아보고자 시행하였다. 방법 : 1998년 1월부터 2002년 4월까지 혈액 배양 검사에서 동정된 K. pneumoniae를 대상으로 NCCLS guidelines과 이중 디스크 확산법(double-disk diffusion test)을 이용하여 ESBL 생성 여부를 확인하였다. ESBL 생성 균주에 의한 균혈증 환자 60명(환자군)에 대해 ESBL을 생성하지 않는 균주에 의한 균혈증 환자들(대조군)을 연령, 성별, 균혈증 발생 시점을 고려하여 1:2 또는 1:3으로 배정하였다. 총 159명의 대조군을 선정하였고 후향적인 환자-대조군 연구를 시행하였다. 결과 : 환자군과 대조군 사이에 연령, 성별, APACHE Ⅱ score, 주된 감염 부위의 유의한 차이는 없었다. ESBL을 생성하는 K. pneumoniae에 의한 균혈증이 발생할 독립적인 위험 인자에는 요관 삽입, 균혈증 발생 이전 72시간 동안 침습적인 시술을 받은 경우, 균혈증 발생 이전 30일 동안 투여받은 항생제 개수가 있었다. 초기 항생제 치료 72시간 후의 반응을 평가하였을 때, 완전 반응(complete response)은 대조군에서 더 많았고(13.3% vs. 40.3%, P<0.001), 치료 실패(treatment failure)는 환자군에서 더 많았다(33.3% vs. 11.9%, P<0.001). 7일 사망률은 환자군에서 20% (12/60), 대조군에서 15.6% (25/159)이었고(P=0.451), 30일 사망률은 환자군에서 30% (18/60), 대조군에서 24.5% (39/159)이었다(P=0.410). ESBL 생성 균주에 의한 균혈증이 있는 환자들에서 최종 항생제 치료가 부적절했던 환자들을 제외하고 30일 사망률을 분석하였을 때 효과적인 항생제 치료의 지연은 사망률을 높이지 않았다(11.1% vs. 9.1%, P=1.000). 결론 : ESBL을 생성하는 K. pneumoniae에 의한 균혈증이 있는 환자에서 초기 항균제 치료 72시간 후의 치료 반응률은 낮지만 사망률은 유의하게 증가하지 않았다. 원인균이 동정된 후 최종 치료 항생제의 선정이 적절하다면 초기에 효과적인 항생제 투여의 지연은 사망률을 유의하게 증가시키지는 않았다. Background : This study was conducted to evaluate risk factors for infection and treatment outcome of bloodstream infection due to extended spectrum β-lactamases(ESBL)-producing K. pneumoniae. Methods: ESBL production was evaluated by NCCLS guidelines and/or double-disk synergy test in K. pneumoniae blood isolates stored from January, 1998 to April, 2002. Sixty patients with bloodstream infection due to ESBL-producing K. pneumoniae (case patients) were compared with 159 matched control patients with bloodstream infection of non-ESBL-producing K. pneumoniae. Retrospective case-control study was performed. Results : There were no significant differences in age, sex, APACHE Ⅱ score, and the primary site of infection between the case and control groups. In multivariate analysis, significant independent risk factors associated with bloodstream infection due to ESBL-producing K. pneumoniae were urinary catheterization, invasive procedure within previous 72 hours, and the number of antibiotics administered within previous 30 days. In clinical response at 72 hours after initial antibiotic treatment, complete response rate was higher in the controls (13.3% vs. 40.3%, respectively, P<0.001), however, treatment failure rate was higher in the cases (33.3% vs. 11.9%, respectively, P<0.001). Overall 7-day mortality rates in the cases and the controls were was 20% (12/60) and 15.7% (25/159) (P=0.451), respectively, and overall 30-day mortality rates were 30% (18/60) and 24.5% (39/159), respectively (P=0.410). When the patients with bloodstream infection of ESBL-producing organism were evaluated and the patients who received inadequate definitive antibiotic treatment were excluded, delayed effective antibiotic treatment was found to be not associated with higher mortality. Conclusion : In patients infected with ESBL-producing K. pneumoniae bacteremia, clinical response rate at 72 hours after antimicrobial therapy was lower, but the increase of mortality rate was not significant. Delayed effective antibiotic treatment was not associated with higher mortality, when definitive appropriate antibiotic treatment was prescribed.

발산풍열약(發散風熱藥)의 항고혈압작용에 관한 연구

金護哲,朴珠瑛,安德均 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

Balsanpungyeolyak, Expelling Pathogenic Wind-Heat Drug, have been used to treat the symptoms such as fever, headache, neck stiffness and skin disturbance which are commonly shown in systemic fever disease like influenza. We thought that the symptoms from the actions of wind and heat in Traditional Korean Medicine are similar to the ones of hypertensive patients in clinic. We chose five drugs among expelling pathogenic wind-heat drugs, Puerariae Radix, Chrysanthemi Flos, Menthae Herba, Mori Folium, and Arctii Fructus, to investigate their antihypertensive effects on S.H.R. and normotensive S.D rats, direct relaxant activity on isolated rabbit aorta, and ACE inhibitory activity. Menthae Herba and Arctii Fructus decreased blood pressure significantly in SHR, but not in normotensive rats, Direct vasodilatative effects on isolated rabbit aorta was not observed in any group. Chrysanthemi Flos, Menthae Herba, Mori Folium, and Arctii Fructus inhibited the actions of ACE dose-dependently. Therefore it is concluded that expelling pathogenic wind-heat drugs has not direct correlation with antihypertensive activity. However, it can be suspected that some drugs for dispersing the actions of wind and heat may regulate blood pressure through ACE inhibition and further study will be helpful to understand the mechanism.