Jo,Kyung-Yul

조경열,최윤수,석경순 대구효성가톨릭대학교 1986 연구논문집 Vol.32 No.1

A new spectropotometric determination of citric acid was examined using carbonyldiimidazole (CDI) which has been used in petide synthesis. CDI was reacted with citric acid to form yellow acyl-imidazole derivative (λ??443nm) in dichloroethane. This colored reaction was so characteristic with tricarboxylic acid that can be used selective determination of citric acid in common carboxylic acid. As increasing the reaction temperature, the acyl compound was decomposed to produce the substance of which physical property was similar to the hydrolized compound. Citric acied was calibrated in the range of 1-6μg/ml and the extinction coefficient of the acyl-imidazole compound was 28000 in this condition.

有機酸의 定量法에 關한 硏究(第一報)

趙庚烈,崔閏壽 대구효성가톨릭대학교 1981 연구논문집 Vol.23 No.3

Carboxylic acid is converted to acid chloride by thionyl chloride. Acid chloride reacts with 2.4-dinitrophenylhydrazine (2.4-D.N.P.H) to form the corresponding hydrazide. this hydrazide in aqueous basic solution leads to color-formation which can be utilized for colorimetric analysis. Since acid chloride is readily hydrolyzed to the parent carboxylic acid in the water, the coupling reaction of acid chloride with 2.4-D.N.P.H is carried out in actonitrile solution. the structures of N-acetyl-, N-benzoyl N'-(2.4-dinitro) phenyl hydrazine formed from the reaction of carboxylic acid and 2.4-D.N.P.H. are investigated by NMR and IR-spectrum.

주걱송편버섯(Pycnoporus cinnabarinus) SCH-3의 Laccase 생산에 미치는 유도원의 영향

조경언,이현수,윤경하 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

This study was performed to examine the effect of inducer on laccase production of Pycnoporus cinnabarinus SCH-3 isolated from Korea. The different inducers including 2,5-xylidine were added in the culture of Pycnoporus cinnabarinus SCH-r respectively. 2,5xylidine was predominant of the inducers tested in laccase production. Addition of 2,5-xylidine enhanced 12-fold laccase production, While nothing of inducers produced isoenzyme of laccase, as determined by non-denaturing polyacrylamide gel electrophoresis.

Ascorbic Acid의 分光光度 定量法에 關한 硏究(第一報)

崔閏壽,趙庚烈 대구효성가톨릭대학교 1981 연구논문집 Vol.23 No.3

A new spectroscopic method was established for the determination of ascorbic acid with indole as a reagent. Indole was reacted with ascorbic acid to form complex compound if the presence of Cu(Ⅱ) ion. The maximum absorbance of the complex compound was at 480nm. Various conditions, (eg, PH, amounts of reagents, temperature of reactants and standing time) were investigated for the maximum colour intensity. Calibration curve for ascorbic acid was linear over a sample of each ascorbic acid in the range of 100㎍/ml. Extinction coefficient was measured, ε=12,592.

구속운동을 받는 외팔보의 혼돈진동

최봉문,류봉조,윤충섭,임경빈 大田産業大學校 2000 한밭대학교 논문집 Vol.17 No.1

When the motion of vibrating structure is restrained by the adjacent objects, its vibration frequencies and mode shapes are changed. In general, the vibration characteristics of the system becomes unpredictable. Although the importance of the study on this type of vibration model increases in many engineering areas, most studies conducted to date were focused to the theoretical study on dynamic responses of the structure with stops, including a few experimental works. Specially, the study on the nonlinear phenomena due to the impact between a structure and stops have been mainly performed theoretically. It is, therefore, desired to find a way to have a better understanding on the vibration characteristics caused by the impact of a structure to stops. In the paper, both numerical analyses and experiments are conducted for a fixed-free beam that is restrained by the stops at the free end. The chaotic vibration characteristics of the nonlinear motion and the dynamic response of the system are investigated and presented for various magnetic forces between stops.

Paraquat Poisoning에서의 hemoperfusion치료 경험

김시환,최재성,윤경우,김영조,최수봉,김종설 대한신장학회 1986 대한신장학회잡지 Vol.5 No.1

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Effect of Jaeumgeonbigagamtang (JGT) on Restraint-induced Oxidative Stress in Mouse Brain

Yoon, Jung-Hun,An, Joung-Jo,Jo, Hyun-Kyung,Son, Chang-Gue,Kim, Yoon-Sik,Seol, In-Chan,Yoo, Ho-Rhyong The Society of Korean Medicine 2011 대한한의학회지 Vol.32 No.6

Objectives: This study was performed to investigate the effect of Jaeumgeonbigagamtang (JGT) onrestraint-induced oxidative stress in the mouse brain. Methods: After treatment with JGT, CBC, ROS, MDA, TAC, SOD, activity of catalase, and total GSH content were analyzed. Results: JGT had a strong antioxidant activity by in vitro assay as presented GEAC. JGT treatment significantly ameliorated decrease of blood WBC and increase of platelet count. JGT (50mg/kg) treatment significantly ameliorated increase of MDA and GSH content level in brain tissue. JGT (100mg/kg) treatment significantly ameliorated increase of MDA and activity of TAC level in brain tissue. JGT (200mg/kg) treatment significantly ameliorated increase of ROS, MDA, activity of TAC level and depletion of catalase level in brain tissue. Conclusion: The present study demonstrated antioxidant activity in brain tissue. This result would be consistent with the long clinical efficacy of JGT, and this finding may provide a strong possibility of JGT as a drug candidate for brain-specific multiple disorders and symptoms.

Ultrastructural Antigenic Localization in the Tissues of Echinostoma hortense Obeserved by Immunogold Labeling Method

Jo,Yoon-Kyung,Ryang,Yong-Suk,Song,Mi-Sook,Lee,Kyu-Je,Ryu,Jang-Keun,Kim,Insik THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCINE 1999 Journal of biomedical laboratory sciences Vol.5 No.2

호르텐스극구흡충을 흰쥐에게 경구 감염시키고 4주 후에 성충을 획득하여 고정, 포매 및 절편하여 조직항원 분포 부위를 관찰하였다. 호르텐스극구흡충의 항체는 호르텐스극구흡충 피낭유충을 흰쥐에게 경구 감염시키고 4주 후에 채혈한 혈청과 호르텐스극구흡충 조항원을 면역증강제와 함께 흰쥐에게 근육 주사하여 채혈한 혈청을 이용하였으며, 이 혈청을 반응시켜서 호르텐스극구흡충 조직내의 항원성 분포를 규명하였다. 한편, 면역황금표식법에 의한 미세조직의 항원성 반응 여부는 조직에 표지된 황금입자로 판정하였다. 그 결과 충체 조직에서 황금입자와 강하게 반응하는 미세조직은 표피층의 표지합포체, 난황세포의 구성물질, 유연조직의 분비과립 그리고 배설낭의 구성물질 등에서 반응이 강하게 나타났다. 이상의 결과를 볼 때 호르텐스극구흡충의 항원은 생식세포와 소화분비 및 소화관계통에서 분비 유래되는 것으로 분석되었다. This study involved applying rat sera (control, infected and immunized) to adult worm tissue in order to measure the antigenic response. A serum was obtained from rats infected with E. hortense metacercaria for 4 weeks. Another immunized serum was taken from rats given a muscle injection with crude adult worm antigen. The detection of antigenic response in E. hortense tissue was made by immunogold labeling method and measured through gold particles impregnated in the tissue. The antigenic sites, those with the highest density of gold particles, were the tegmental syncytium, vitelline cells, seminal receptacle and cecum.

Detection of Salmonella typhi by Loop-mediated Isothermal Amplification Assay

Yoon-kyung Jo,Chang-Yeoul Lee 대한의생명과학회 2008 Biomedical Science Letters Vol.14 No.2

Salmonella typhi is frequent causes of foodborne illness and its detection is important for monitoring disease progression. In this study, by using general PCR and novel LAMP (Loop Mediated Isothermal Amplification) assay, we evaluated the usefulness of LAMP assay for detection of Salmonella typhi. In this LAMP assay, forward inner primer (FIP) and back inner primer (BIP) was specially designed for recognizing target invA gene. Target DNA was amplified and visualized as ladder-like pattern of bands on agarose gel within 60 min under isothermal conditions at 65℃. When the sensitivity and reproducibility of LAMP were compared to general PCR, there was no difference of reproducibility but sensitivity of LAMP assay was more efficient than PCR (the detection limit of LAMP assay was 30 fg, while the PCR assay was 3 pg). These results indicate that the LAMP assay is a potential and valuable means for detection of Salmonella typhi, especially for its rapidity, simplicity and low cost.