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- 저자 : ( Yongkai Ma ) (검색결과 3 건)
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In Vivo/In Vitro Properties of Novel Antioxidant Peptide from Pinctada fucata
( Yongkai Ma ),( Kehui Huang ),( Yanyan Wu ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.1
Due to the potential of antioxidants to scavenge free radicals in human body, it is important to be able to prepare antioxidant peptides that meet the industrial requirements for cosmetics and food. Here, we determined in vivo/in vitro activities of antioxidant peptide from P. fucata (PFAOP) prepared by bio-fermentation method. The antioxidant property test results showed the DPPH, hydroxyl, superoxide radical-scavenging, and cellular antioxidant activity. EC50 values of PFAOPs were 0.018 ± 0.005, 0.126 ± 0.008, 0.168 ± 0.005, and 0.105 ± 0.005 mg/ml, respectively, exhibiting higher antioxidant activities than glutathione (p < 0.05). Moreover, anti-proliferation and cytotoxicity activity results illustrated PFAOP has a potent anti-proliferative activity against HepG2, Caco-2, and MCF-7 carcinoma cells with no cytotoxicity. Moreover, the protocols we developed in this work demonstrated several excellent advantages in PFAOP preparation compared to enzymatic hydrolysis or chemical synthesis methods and provide a theoretical foundation for higher-value application of marine-derived functional peptides.
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( Yanyan Wu ),( Yongkai Ma ),( Laihao Li ),( Xianqing Yang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.1
An antioxidant peptide derived from Pinctada fucata meat using an Alcalase2.4L enzymatic hydrolysis method (named AOP) and identified by LC-TOF-MS has promising clinical potential for generating cosmetic products that protect skin from sunshine. To date, there have been few published studies investigating the structure-activity relationship in these peptides. To prepare antioxidant peptides better and improve their stability, the design and expression of an antioxidant peptide from Pinctada fucata (named DSAOP) was studied. The peptide contains a common precursor of an expression vector containing an α-helix tandemly linked according to the BamHI restriction sites. The DNA fragments encoding DSAOP were synthesized and subcloned into the expression vector pET-30a (+), and the peptide was expressed mostly as soluble protein in recombinant Escherichia coli. Meanwhile, the DPPH radical scavenging activity, superoxide radical scavenging activity, and hydroxyl radical scavenging activity of DSAOP IC50 values were 0.136 ± 0.006, 0.625 ± 0.025, and 0.306 ± 0.015 mg/ml, respectively, with 2-fold higher DPPH radical scavenging activity compared with chemosynthesized AOP (p < 0.05), as well as higher superoxide radical scavenging activity compared with natural AOP (p < 0.05). This preparation method was at the international advanced level. Furthermore, pilot-scale production results showed that DSAOP was expressed successfully in fermenter cultures, which indicated that the design strategy and expression methods would be useful for obtaining substantial amounts of stable peptides at low costs. These results showed that DSAOP produced with recombinant Escherichia coli could be useful in cosmetic skin care products, health foods, and pharmaceuticals.
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Zhang Hao,Liu Yu,Li Jingyuan,Yu Hanzhengnan,Xu Hang,Ma Kunqi,Liang Yongkai,An Xiaopan,Hu Xi 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.5
Under the background that electric vehicles cause enormous supply pressure on the grid, the application of vehicle-to-grid (V2G) technology can effectively alleviate supply pressure and also bring more economic benefits. Considering conditions for participating in V2G, pure electric logistics fleets have the advantages of stable work end time and large remaining power. In this paper, the economic benefits of the pure electric logistics fleet participating in V2G and the influence factors of the V2G profit are studied. Firstly, the travel data of a pure electric logistics fleet in Chengdu, China is collected. Secondly, the V2G profit model is established to quantify the economic benefits. Then, 4 V2G scenario modes are proposed based on window duration, window start time, SOC lower limit and discharge price. Finally, the change law and influence factors of the V2G profit in each V2G scenario mode are studied from the perspective of the discharge revenue, charge cost and battery loss cost. The results show that the V2G profit increases with the extension of window duration, the delay of window start time, the decrease of SOC lower limit and the increase of discharge price. The average profit of logistics fleets ranges from 3.94 RMB/(vehicleday) to 65.54 RMB/(vehicleday).
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