Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

강변여과에서 파일럿규모 수평집수관의 수두분포 연구

정재민(Jae Min Jeong),박재영(Jae Young Park),이종진(Jong Jin Lee),김용운(Yong Woon Kim),김승현(Seung Hyun Kim) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.5

강변여과용 수평집수관을 모사하는 파일럿 규모의 모래통 실험을 수행하였으며, 다양한 출구유속에 대한 수평집수관에서의 수두분포를 구하였다. Kim24)이 수행한 실험실 규모의 수평집수관에서의 손실수두에 Kim25)이 개발한 이론 즉, 작은 규모의 수평집수관에서의 손실수두로부터 큰 집수관의 손실수두를 계산하는 방법을 적용하여 본 연구의 관측치와 비교함으로써 Kim25)의 이론이 타당함을 알 수 있었다. 또한, 이들의 이론을 적용하여 본 연구에서의 관측치로부터 실규모 집수관에서의 수두분포를 구하는 방안을 예를 들어 설명함으로써 강변여과의 수평집수관 설계방법을 제시하였다. A pilot-scale sand-box experiment simulating a lateral of collector well used in riverbank filtration was performed, and hydraulic head distributions along the lateral were measured according to the various drawdowns at the well. The results of this experiment were compared to the predicted values obtained using a theory previously developed by Kim25) in order for its validation. This theory predicts the head loss in a large-scale lateral given the loss in a small-scale one, and to have actual values for comparison, the results from a lab-scale experiment previously performed by Kim24) were employed. Comparing the experimental values to the extrapolated value indeed confirmed the validity of the theory. A procedure to determine the hydraulic head distribution of a practical-scale lateral was also presented applying the theory to the experimental result of this study in an effort to show the process of lateral design for riverbank filtration.

C 의과대학에서 문제강의에 대한 학생들의 직접평가가 강의개선에 미친 영향

김석용, 이상진, 최재운, 김승렬, 김용대, 배성은, 김영규 충북대학교 의과대학 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.1

연구목적: 팀티칭에 근거한 통합강의는 최근 대부분의 의과대학에서 시행되는 강의형태이다. 그러나 기 존에 사용되고 있는 온라인 강의평가 방식은 이러한 강의 특성에서 나타나는 문제점을 파악하기 힘든 점이 있다. 본 대학에서는 팀티칭에 의한 통합강의에서 문제강의를 직접적으로 파악하는 ‘문제강의 직 접평가지’를 개발하였다. 본 연구의 목적은 새로이 개발된 문제강의 직접평가지가 강의개선에 끼친 영 향력을 알아보고자 하는 것이었다. 연구방법: 2007년 2학기부터 2009년 2학기까지 충북대학교 의과대학 1, 2학년을 대상으로 문제강의 직접평가지에 의한 강의평가를 실시하였다. 평가지 질문 중에서 두 가지 객관식 질문에 대한 답변을 분 석대상으로 선택하였다. 질문은 첫째, ‘가장 유익하고 도움이 많이 되었던 강의들은 무엇입니까?’와 둘 째, ‘가장 어렵거나 이해하기 힘들었던 강의들은 무엇입니까?’였다. 강의평가를 실시한 강좌들 중에서 2 년 이상 연속적으로 강좌구조와 평가지의 객관식 질문 형식이 변하지 않은 14개의 강좌를 분석대상으로 하였다. 각각 연도의 평균 긍정비율을 다른 연도의 것과 카이제곱 검정 방법을 사용하여 비교하였다. 결과: 14개의 강좌 중에서 시작연도와 마침 연도 간에 긍정비율이 증가된 경우는 13개 강좌였다. 그 러나 통계적으로 의미가 있는 경우는 5개 강좌에서 나타났다. 결론: 문제강의 직접평가지에 의한 문제강의를 판별해 내는 방법은 단기간에 통합강좌의 개선에 효과 적이다.

Accuracy on the Edge Detection of Topography for Digital Map Manufacture

Park, Woon-Yong,Song, Youn-Kyung,Bae, Kyung-Ho,Kim, Yong-Suk 東亞大學校 建設技術硏究所 2005 硏究論文集 Vol.28 No.2

Currently, digital maps in the metropolitan area are supposed to be revised and renewed every two years, but it is difficulty for the renewal of current information to keep pace with the quickly changing society and so, one of the alternative methods being discussed is a digital map produced using high resolution satellites. The biggest problem in the production of digital maps using high resolution satellite images is the difficulty of production in vector resource format through edge detection of satellite images, a Raster data. In this study, topographical boundaries were detected through edge detection of the satellite image, and after vectorliezing using the screen digitizing method, the results were evaluated and compared to the digital map from the NGII(National Geographic Information Institute) for accuracy.

부산시 용호동 정책이주단지 주거공간의 내·외부 변용 특성에 관한 연구

김용운(Kim, Yong-Woon),유재우(You, Jae-Woo) 대한건축학회 2014 대한건축학회 학술발표대회 논문집 Vol.34 No.2

電解法에 依한 Polyvinyl Alcohol 含有 廢水處理에 關한 硏究

김운수,차시환,김용욱 慶熙大學校 材料科學技術硏究所 1991 材料科學技術硏究論集 Vol.4 No.-

The purpose of this study is to investigate into the electrolytic treatment of polyvinyl alcohol wastewater. In order to remove PVA from wastewater by electrolytic treatment, Fe electrode was used as a soluble electrode for this study. The results of this sutdy are as follows: 1. PVA was removed nearly to 100% as the value of ampere hour concentration was more than 0.5 Ahr/ℓ. 2. PVA was removed quickly as the current density was rised, the electrode distance was approached, NaCl was added and the initial pH of wastewater was above 7. 3. Regardless of the initial pH of wastewater, the pH of wastewater after electorolytic treatment was among 10 and 11. So, it is necessary to neutralize the treated water.

Stretch-Shortening Cycle과 수행력 향상 : SSC에 의한 수행 강화와 요인, 기전을 중심으로

김용운,이영석 서울대학교 스포츠과학연구소 2007 스포츠과학리뷰 Vol.1 No.1

A great portion of human movement involves specific muscle action refered to as the "stretch-shortening cycle (SSC)", such as walking, running, jumping and throwing etc.. In this study the pattern, effects, concepts, factors and mechanisms are reviewed. The SSC is the process of the muscle stretching eccentrically followed by concentrically shortening. In the majority of research comparing the concentric only cycle (COC) and the SSC, there is an substantial increase in mechanical output, power and strength with the SCC observed. The proposed reason for this, is that when the stretch precede concentric contraction, elastic energy can be stored and reutilized in the concentric phase. Therefore the stored energy is available for more powerful movements in the concentric phase and so performance is enhanced more effectively and efficiently. The delay between stretching and shortening must be reduced to increase the effectiveness of the SSC. In other words, the amplitude of stretching and velocity of stretching needs to be as high as possible in limited area. Not only is this factor important, but so are the muscle's stiffness, type of muscle fibers, individual skill levels and movement strategy. The explanation of the SSC's effects are as follows. 1) Elastic Potentiation - the stored elastic energy during stretching is re-utilizated in the final phase. 2) Force Potentiation - is created by the change in mechanical properties due to the muscle's conctractile mechanism. 3) More time is available to produce more a higher level of force in terms of the muscle stimulation-excitation-contraction dynamics. 4) Myoelectrial Potentiation - an increase of muscle stimulation by the stretch reflex which is generated through the change of muscle length during the stretch. However, the amount of contribution from each factor towards the increased performance due to the SSC is still under debate and a more effective method for utilizing SSC must be further investigated.

높이뛰기競技의 評價基準에 關한 硏究

金永煥,崔龍雲,朴鉉權 慶北大學校 師範大學 體育學硏究會 1990 體育學會誌 Vol.18 No.-

We gained the results like next, according to the analysis and measurement of records on High Jump, in male 467, female 74, from 1980 to 1989(for 10 years). (1) We gained the distribution chart like (figure 3) (figure 4) and also We gained the average Value like (figure 5). (2) We gained a new Standard Value of evaluation High Jump like(figure 9). (3) As we know in(figure 10) In the result of Comparison between old and new standard Values, We can gain following, a) In the cases of male, it is increase by average 2㎝. b) In the cases of female, the Evaluational of student gained record 115㎝, old Standard is Score of 80, but new Standard is decrease of score 5 by score 75. From 1991 One's junior Season, We decided to apply this new Standard Value.

표면 딤플 유무에 따른 골프공 주위 유동장의 해석

김봉근,이경용,박운진 한국기술교육대학교 2003 論文集 Vol.10 No.1

To investigate the drag reduction effect of surface dimples on a golf ball, the flow fields around golf ball models with or without dimples at a subcritical Reynolds number of Re=1.4x10⁴ were analyzed experimentally in a wind tunnel as well as numerically by utilizing the commercial CFD program of CFX 5.5. It was proved by hot-wire measurements and flow visualization that the onset of flow separation was obviously delayed by the existence of surface dimples on a golf ball and thus thewake width of the flow field behind a golf ball with dimples became slightly narrower than that without dimple. For both cases, it was hard to identify the Karman type vortex shedding frequency and its harmonics in the wakes. At this relatively high Reynolds number, it was revealed that the implementation of a commercial CFD program to predict the flow field around a 3-D blunt body was not fruitful.

고순도 단백질 재료 분리를 위한 Lysozyme 농도분석에 관한 연구

김운수,김우식,김용욱 慶熙大學校 材料科學技術硏究所 1995 材料科學技術硏究論集 Vol.8 No.-

In this study the lysis reaction of lysozyme to Micrococcus lysodeikticus cell(ML cell) was investigated to develop the quick and accurate assay technique for lysozyme concentration. For the investigation of the lysis reaction of lysozyme the Michaelis-Menton(M-M) reaction mechanism was applied and Lineweaver-Burk plot was used to calculate the reaction rates. The concentration of lysozyme and ML cell were changed from 2.0 to 10.0mg/l and from 10 to 50mg%, respectively. In these range of concentrations of the lysozyme and ML cell the lysis reaction was good agreement with M-M reaction mechanism and then the maximum reaction constant and Michaelis-Menton constant were measured as 1.40×10^(-2) and 11.08, respectively. These information on the lysis reaction contributes to the better analysis of lysozyme concentration in comparison with conventional procedure.