Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

요부 외측구역 협착증에서 단순감압술과 기구고정술을 병행한 요추체간 골유합술과의 임상적 비교

손병길,최은석,정을수,신종현,김명진,지용철 대한척추신경외과학회 2004 Neurospine Vol.1 No.1

Objective: The lumbar spinal canal can be devided into the central zone and the lateral zone. The divisions of the lateral zone are the subarticular, the foraminal, and the extraforaminal zone. A common causes of the operation for the spinal stenosis are related to the association with the spondylolisthesis and the central stenosis. There is a paucity of data, however, regarding the operative intervention in the lateral zone stenosis(LZS). Our aim was to analyze the surgical result by the anatomical type and the operative method. Methods: 72 patients of LZS excepting the association with the spondyolisthesis have been operated by six neurosurgeons of our hospital from 30th April, 1994 through 30th September, 2002. The patients had been interviewed about surgical result through telephone by third party persons. The surgical results were analyzed by the four score rating system: ① medication, ② ADL(Ability of daily living), ③ the degree of improvement, ④ the degree of satisfaction. Results: Among 72 patients, there were 25 males and 47 females. The age group of the patients were in their thirties(1), forties(9), fifties(21), sixties(36) and seventies(5). Surgical methods were used on decompression(22), posterior lumbar interbody fusion(PLIF) with cage(1) and PLIF with transpedicular screw fixation system(49). The types of stenosis were subarticular(24), foraminal(16) and extraforaminal(30) and double crush(2). The overall surgical results were Excellent(18), Good(34), Fair(19), and Poor(1). The Non-success rate according to the methods of surgery were decompression(34.8%), PLIF with transpedicular fixation system(24.4%). The overall success rate was(53/72: 73.6%). The success rate according to the types were subarticular(17/24: 70.8%), extraforaminal(21/30: 70%), and foraminal(12/16: 75%). Conclusion: It is suggested that the result was more favorable on the cases of lateral zone stenosis operated with PLIF and transpedicular fixation system. According to the method used, the success rate of decompression was worse than PLIF with transpedicular fixation system.

Urapidil, Labetalol의 투여가 기관내 삽관시 심혈관계에 미치는 영향

신용섭,윤석화,손수창,이원형,이정은,황원재,김만수,김영주,김혜자,최세진 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

We have examined the comparative efficacy of small doses of intravenous urapidil and labetalol in blunting hemodynamic response to endotracheal intubation and surgical incision in 30 patients without cardiovascular diseases. After intravenous urapidil 0.2 mg/kg or labetalol 0.2 mg/kg anesthesia was induced with thiopental 5mg/kg. Endotracheal intubation was facilitated by vecuronium 0.15 mg/kg with priming principle and anesthesia was maintained with enflurane and nitrous oxide in oxygen. Systolic, diastolic and mean arterial pressure and heart rate were measured before administration of the drugs, 5 minute after administration, just prior to endotracheal intubation and 1, 3, 5, 10 minute after intubatin. Also the peak blood pressures and heart rate within 10 minutes after surgical incision were measured. Endotracheal intubation and surgical stimulation were associated with significant increases in blood pressures and heart rate in both urapidil and labetalol group. Comparison of the changes in systolic, diastolic, and mean artrial pressures and heart rate between urapidil and labetalol group showed no significant difference except peak systolic pressure after surgical incision. It is concluded that the pressor response to endotracheal intubation and surgical stimulation are not influenced significantly by urapidil 0.2 mg/kg or labetalol 0.2 mg/kg. However, urapidil and labetalol preloading may be similarly effective in the blunting of the increases in blood pressures with larger doses of the durgs during anesthetic induction.

鹽化物 含有梁에 따른 鐵分 腐蝕에 관한 實驗的 硏究

최진석,조철희,신용석,고송균,손순채,김정섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

As a result of separating river sand and sea sand into the corrosion increased according to the of thinnss or the coating and the Chloride Content and Corrosion of Steel Bar through the experiment, the following conclusions are obtained. 1. There was no corrosion in the case of treating the steel bar with anticorrosive among test specimens cured in the air for 60 days, corrosion was found in the test specimens not treated with anticorrosive and for 60 days of age. 2. The area of corrosion increased according to the thinnss of the coating and the chloride content wher exposed for are more than 60 days. Therefore, it was determined in general that the corrosion progressed more rapidly in water cured specimens than in air cured ones and this was due to the influence of C1_(￣) in the sea sand.

Time Frequency Domain Reflectometry 기법을 이용한 Coaxial Cable에서의 결함 감지 및 추정

송은석,신용준,최덕선,육종관,박진배,Powers, Edward J. 한국항행학회 2003 韓國航行學會論文誌 Vol.7 No.1

본 논문에서는 도선사에서 발생하는 결함 위치와 이상 유무를 감지하는 새로운 고분해능 반사측정법인 시간-주파수 영역반사측정법 (TFDR, Time-Frequency Domain Reflectometry)을 제안하였다. 고전적인 반사측정법들은 단지 시간 또는 주파수의 한 영역에서 분석되어져 왔으나, 본 논문에서 제시한 TFDR은 도선의 결함 위치와 이상 유무를 발견하기 위해 과도신호의 시간과 주파수 영역의 정보를 동시에 이용할 수 있는 시간-주파수 분석기법으로 특성화하였다. TFDR의 기준신호 설계는 측정 케이블의 물리적 성질들을 고려하여 주파수 밴드를 결정하며, 도선의 결함감지와 추정은 시간-주파수 상호상관관계 함수에 의해 이루어진다. TFDR 시스템을 이용하여 여러 결함 상태를 가진 실제 coaxial cable (RG-142, RG-400)에 대해 실험하였고 정확성을 입증하기 위해 TDR (Time Domain Reflectometry) 장비와 성능은 비교하였다. 본 논문에서는 TFDR이 TDR보다 작은 오차로 결함을 찾아냄을 나타내고 있으며, 측정된 정확도는 TFDR의 오차율이 0.5%이하로 TDR (54750A/54754A) 장비보다 성능이 월등히 우수하다는 것을 알 수 있다. In this paper, a new high resolution reflectometry scheme, time-frequency domain reflectometry (TFDR), is proposed to detect and locate fault in wiring. Traditional reflectometry methods have been achieved in either the time domain or frequency domain only. However, time-frequency domain reflectometry utilizes time and frequency information of a transient signal to detect and locate the fault. The time-frequency domain reflectometry approach described in this paper is characterized by time-frequency reference signal design and post-processing of the reference and reflected signals to detect and locate the fault. Design of the reference signal in time-frequency domain reflectometry is based on the determination of the frequency bandwidth of the physical properties of cable under test. The detection and estimation of the fault on the time-frequency domain reflectometry relies on the time-frequency domain reflectometry is compared with commercial time domain reflectometry (TDR) instrument. In these experiments provided in this paper, TFDR locates the fault with smaller error than TDR. Knowledge of time and frequency localized information for the reference and reflected signal gained via time-frequency analysis, allows one to detect the fault and estimate the location accurately.

다양한 놀이의 장을 통한 분수와 소수의 지도 방안

송장석,손용규 晋州敎育大學校 科學敎育硏究所 1992 科學敎育硏究 Vol.18 No.-

구체물 자료를 통하여 놀이의 장을 이용하여 분수, 소수의 계산 능력을 기르기 위하여 조작활동을 통해 원리, 계산, 법칙을 발견하도록 하고 스스로 해결할 수 있는 지도 방안을 제시하고자 하였다. 이와 같은 목적을 통해 실행 목표는 1) 분수와 소수에 대한 학습 목표를 제시한다. 2) 분수와 소수에 대한 학습 목표를 제시한다. 3) 다양한 놀이의 장을 통한 분수와 소수에 대한 자료의 개발과 활용 방법을 구안한다. 4) 분수와 소수의 기본 개념과 원리의 지도에 있어서 학습 단계에 따라 놀이의 장을 투입한다. 연구의 방법으로는 1) 대상은 본교 5학년 연구반 46명, 비교반 47명으로 2) 연구반은 놀이의 장을 통한 분수 소수의 지도, 비교반은 종래의 설명식 지도 방법으로 실시, 3) 처치도는 놀이의 장을 활용하고 4) 단원으로는 5의 1학기 3개 단원, 2학기 3개 단원으로 하고 5) 도구로는 학습 평가 문항으로 개념, 원리, 법칙, 적용 능력을 측정하였다. 6) 수집된 자료는 놀이의 장 수업에 의하여 학습하는 학생은 설명식 수업보다, 보다 높은 수준의 지식인 일반화와 개념을 더 많이 획득하는 것으로 나타났다.

先加力 후 補修-補强한 鐵筋콘크리트 壓縮部材의 構造特性에 관한 實驗的 硏究

신용석,최진석,김판선,조철희,손순채,김정섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

This study examined the Stress and structural characteristics of reinforced concrete compressive member repair and strengthening by CFS and GFS after pre-loading. The following results were obtained. The results of compressive testing by the kind of fiber suggested that: In CFS, Specimen increased by about 26.9% and strengthening after pre-loading, by about 111.8%, compared with after strengthening, In GFS, Specimen increased by about 69.0% and repaired and models after pre-loading, by about 76.7%, compared with after strengthening. In the compressive testing, strengthen with CFS-Specimen showed a brittle fracture and strengthen with GFS-Specimen represented ductile fracture. More increasing in stress of Specimen, and repair and Strengthening specimen after pre-loading than loading specimen after strengthening suggested the strengthen effect of fiber.

Midazolam의 백서 기관평활근 이완효과와 Flumazenil의 길항작용

윤석화,신용섭,손수창,이원형,김혜자,최세진 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

Midazolam have been used widely as adjuvants in both regional and general anesthesia and in high risk patients in the intensive care unit. In the present study we evaluated the effect of midazolam and antagonistic effect of flumazenil on isolated rat tracheal preparations mounted for recording isometric contractile force. Trachea smooth muscle were contracted with acetylcholine (10^-5M, Ach) and potassium chloride (40mM KC1). Midazolam 5x10 exp (-6)M and 5x10 exp (-5)M produced dose-dependent relaxation and flumazenil (3.2 x 10 exp (-7)M), central antagonist of benzodiazepine, pretreatment had no significant change midazolaminduced relaxation. Midazolam probably did not relax airway smooth muscle by activatong central benzodiazepine receptors, as flumazenil did not block the concentration-related relaxation of midazolam. Our study suggests that flumazenil may be used satety for asthmatic patients who have been with midazolam, as flumazenil did not antagonize airway relaxation elicited by midazolam.

정맥내 통증자가조절법을 이용한 Nalbuphine-Ketorolac과 Butorphanol-Ketorolac의 술후 진통효과 비교

윤석화,이원형,손수창,신용섭,김윤희,양신영 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

This study aimed to compare analgegic efficacy, satisfaction score and side effect of nalbuphine-ketorolac and butorphanol-ketorolac by using intravenous patient controlled analgesia(IV-PCA) for postoperative anlgesia following a gastrectomy for stomach cancer. Ninety patients who had undergone gastrectomy for stomach cancer under general anesthesia were randomly divided into two groups. Each group recieived nalbuphine 50mg with ketorolac 150mg(Group 1, n=45) and butorphanol 5mg with ketorolac 150mg(Group 2, n=45) by using IV-PCA during postoperative 48hrs. Assessments for pain with numerical rating scale(NRS), and side effects were evaluated at 2hr, 6hr, 12hr, 24hr, 36hr and 48hr after the operation. There were no significant difference in NRS for pain during rest, but Group 2 requested significantly greater amount of supplementary diclofenac during first 24 hours. Side effects were higher Group 1 in pruritus, nausea and vomiting and Group 2 in sedation, nausea and vomiting. This study suggests that adding ketorolac with intravenous nalbuphine or butrophanol in using an intravenous PCA can decrease analgesics requirement and improve analgesic property without the major morbidity like respiratory depression, but needs for the careful observation and treatment on the side effects like nausea. vomiting, pruritis and sedation...

대장암 환자에서의 담즙 암태아성 항원(CEA)

김종우,박용태,정철운,권성원,장항석,손석우,안대호,이경포 대한대장항문학회 2000 Annals of Coloproctolgy Vol.16 No.6