대동맥 이첨판과 승모판 이탈증을 동반한 대동맥 판하부 협착 1예

김용화,장경식,양태영,임영국,안기완,홍순표 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Discrete subaortic stenosis is a rare cardiac disorder especially in Orientals which occupies eight to ten percent of aortic stenosis, It occurs mainly before age of eighteen and accompanies with aortic valve abnormalities such as aortic stenosis, supravalvrlar stenosis, bicuspid valve and muscular hypertrophic subaortic stenosis, and other cardiac abnormalities such as ventricular septal defect, pulmonary stenosis and coarctation of aorta. There is no report of discrete subaortic stenosis accompanied with mitral valve prolapse. The major complications of discrete subaortic stenosis are aortic insufficiency, and subacute bacterial endocarditis. We report a case of asymptomatic nineteen-year-old male of discrete subaotic stenosis with bicuspid aortic valve and mitral valve prolapse, which is comfirmed by two-dimensional and Doppler echocardiography including transesophaigeal echocardiography.

유전자 검색을 위한 DNA 칩 제작용 microarrayer의 개발

이현동,김기대,김찬수,임용표,박정규 충남대학교 농업과학연구소 2003 농업과학연구 Vol.30 No.1

외국의 경우 게놈 연구 및 바이오 산업에 DNA 칩을 제작할 수 있는 로봇 시스템을 싼 가격에 사용하고 있으나 우리나라의 경우 자동화 시스템을 비싼 가격에 외국에서 도입하여 사용하기 때문에 바이오 산업 및 연구 분야에서의 생산비를 높이게 돼 국내외적으로 생명공학의 경쟁력을 저하시키는 원인이 된다. 따라서, 본 연구에서는 유전체 연구에 필수적인 DNA 칩 제작을 위한 연구용 pin 타입 microarrayer를 개발하였으며, 그 구체적인 연구결과는 다음과 같다. 1. 본 연구에서는 DNA칩 제작을 위한 연구용 pin 타입 microarrayer를 개발하였으며 3축 직교좌표형 로봇 본체 DNA를 묻혀 silylated 슬라이드에 점착하는 DNA 점착 헤드, 칩 및 웰 플레이트 고정부, 핀을 세척 및 건조하는 세척 및 건조장치 등으로 시스템을 구성하였다. 2. DNA 점착 헤드는 DNA 점착시 제도용 펜촉을 사용하도록 설계, 제작하였으며, 슬라이드에 DNA를 점착할 때는 핀이 일정한 힘으로 슬라이드를 누르며 점착할 수 있도록 자석의 반발력을 이용하였다. 3. DNA 점착 헤드 핀의 세척을 위하여 증류수 분사 및 진동 브러쉬를 이용하였으며 세척실험 결과, 핀을 1㎜/s로 이동시키며 브러쉬를 통과하도록 하는 방법이 세척효과가 높은 것으로 나타났으며, 핀 건조실험결과는 8.5㎏/㎤의 압축공기를 30초 동안 핀에 분사하였을 때 핀이 건조되는 것으로 나타났다. 4. 본 로봇 시스템을 이용하여 DNA를 12장의 슬라이드에 모두 점착시키기 위하여 웰 플레이트에서 핀이 DNA를 묻히는 실험을 실시한 결과, 10초 이상 핀에 DNA를 묻혔을 때 슬라이드 12장을 모두 찍는 것으로 나타났으며, 슬라이드에 핀이 1초간 접촉할 때의 DNA 스팟의 크기는 평균 280㎛가 되는 것으로 나타났다. 최소 점 간격을 0.32㎜로 설정한 후 DNA를 점착해 본 결과 최대 8,100여 점의 DNA 스팟을 한 슬라이드에 점착할 수 있는 것으로 나타났다. 5. 본 로봇 시스템은 12장의 동일 DNA 칩을 생성하기 위해 핀의 세척, 건조, DNA를 묻히는 과정 및 DNA 점착 등의 한 과정을 2분 50초 동안 수행할 수 있는 것으로 나타났다. This study exploits the robot system which is necessary in gene study, bio-technology industry. As well, it can achieve the job of DNA chip manufacturing whose use rate has been increased recently. The robot consists of DNA spotting device for spotting DNA on the silylated slide and well plate, bed for fixing well-plate, washing & drying device of washing and drying the pin part of DNA spotting device, distillation-water vessel, and discharge vessel of wash water. We made the term of sticking DNA to the pin on well plate to be 15 seconds. The spot size of DNA was set to be 0.28㎜ on the average by bringing the slide into contact with pin for 1 second. At this rate, if DNA is spotted in the minimum space possible of about 0.32㎜, it can stick about 8,100 DNA spots on the well plate. Analyzing the procedure: Movement starts. Pin washes, dries, and smears DNA on the well plate. Spots DNA onto 12 chips takes 2 minutes and 50 seconds.

중이온 빔 조사에 의한 담배의 돌연변이 유도와 내염성 식물의 선발

배창휴,Abe, Tomoko,민경수,김동철,정재성,이춘환,임용표,이효연 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

중이온 빔을 이용한 방사선 돌연변이의 기초자료를 얻고자 담배의 수분·수정 직후의 원배에 100 Gy의 중이온빔을 조사하여 M_1세대의 종자를 얻었다. NaCI 2.0과 2.5%를 포함된 MS배지에 M_1 세대의 종자를 파종하여 17개통의 NaCI 저항성 식물체를 선발하였다. 저항성 식물체는 BY-4품종에 ^14N빔을 조사한 처리구에서만 관찰되었다. M_1 세대의 저항성 식물체의 경우 영양생장기간 중에는 외형적 변이가 관찰되지 않았으나 생식생장기의 화기에 있어서는 다양한 변이가 관찰되었다. 특히 암술 수술의 길이의 변화, 꽃잎의 변화, 불완전 화분립, 줄기당 꽃의 착생수 감소 등의 변이가 발생하였다. M_1 세대에서 17개의 NaCI저항성 계통 중에서 8계통은 M_2 세대에도 저항성형질이 발현되었고, 또한 M_2 세대의 3계통은 Mannitol이 포함된 배지에서 저항성을 보여주었다. Tobacco proembryos were irradiated with 100 Gy of heavy-ion beams (^14N, ^20Ne : 135 Mev/u) after 24 to 96 hours of pollination as a mutagen and screened M_1 generation for morphological mutants and salt-tolerant plants. Morphological and physilolgical characteristics of the salt-tolerant plants derived from the irradiated proembryo are discussed in this report. Mutants irradiated porembryos with the beams after pollination produced various kinds of morphological variation. A total of 17 salt-tolerant plants were selected from tobacco cultivar (BY-4) by treatment with ^14N beam. Shapes of filament and pollen grain of most salt-tolerant plants were abnormal compared with non-irradiated wild type, and seeds weight and fertility obviously decreased. The germination rates of the several M_2 lines on the saline and the mannitol condition were higher than that of wild type.

Agrobacterium tumefaciens를 이용한 bialaphos 저항성 형질전환 벼의 개발

이효연,이춘환,김호일,한원동,최지은,김진호,임용표 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

비선택성 제초제인 bialaphos는 고등식물에 있어서 glutamine 합성을 억제하여 식물체를 고사시킨다. Acetyltransferase에 의해 encoding된 bialaphos 저항성 유전자는 세균 Streptomyces hygroscopicus SF1239로부터 cloning된 것을 사용하였다. Bialaphose 저항성 유전자를 Agrobacterium 감염법을 이용하여 국내에서 재배되는 벼(동진)에 도입한 결과 약 30%정도의 형질전환 식물체를 얻을 수 있었다. T_1세대의 17개체는 hygromycin과 bialaphos에 대한 저항성 유전형질이 3 : 1로 분리되었다. 또한 Southern 분석을 실시한 결과 wild type의 식물체에서는 Bar 유전자의 검출을 볼 수 없었으나 형질전환 식물체의 경우 Bar 유전자의 검출이 가능하였다. T_3 세대의 형질전환 식물체와 wild type의 식물체를 포장상태에서 비선택성 제초제인 바스타를 살포하고 3주 후에 관찰한 결과 형질전환 식물체는 외형적으로 아무런 피해를 받지 않고 정상적으로 생장하였으나, wild type의 식물체와 잡초는 모두 고사하였다. 이상의 결과를 종합해보면 hygromycin과 bialaphos 저항성 유전자는 Agrobacterium 감염법을 이용하여 단자엽 식물인 벼에 도입할 수 있다는 것을 보여준 것이며, 또한 bialaphos 저항성 유전자가 식물에 도입됨으로써 비선택성 제초제에 대한 저항성 식물을 개발 할 수 있다는 것을 보여 주었다. The bialaphos is a potent inhibitor of glutamine synthease in higher plants and is used as a non-selective herbicide. We have used the bialaphos resistant gene (Bar) encoding for an acetyltransferase isloated from Streptomyces hygroscopicus SF1293. Callus derived from mature seeds of rice (Oryza sativa L. cv. Dong Jin) were co-cultivated with Agrobacterium tumefacieus EHA101 carring a plasmid pGPTV-HB containing genes for hygromycin resistance (HygR) and Bar. Transgenic plants showing in vitro resistance to 50 mg/L hygromycin and 10 mg/L bialaphos were obtained by using a two-step selection/regeneration procedure. Transformation efficiency of rice was about 30% which was as high as reported in other dicotyledons. Progenies (T_1 generation) derived from primary transformant of 17 lines were segreagated with a 3 resistant:1 sensitive ratio in medium containing hygromycin and bialaphos. Stable integration of Bar gene into chromosomal DNA was proven by Southern blot analysis of genomic DNA isolated from T_2 progenies. Transgenic plants (T_3) grown in the field were resistant to bialaphos (Basta) at a dosage lethal to wild type plants.

An efficient transformation system for zoysiagrass (Zoysia japonica steud.) mediated by Agrobacterium tumefaciens

Chang Hyu Bae,Yong Pyo Lim,Pill Soon Song,Taiji Adachi,Jeong Gu Kang,Kohichi Tohyama,Hyo Yeon 한국육종학회 2001 한국육종학회 학술발표회 발표요지 Vol.2001 No.9

Toward unraveling the structure of <i>Brassica rapa</i> genome

Lim, Yong Pyo,Plaha, Prikshit,Choi, Su Ryun,Uhm, Taesik,Hong, Chang Pyo,Bang, Jae Wook,Hur, Yoon Kang Blackwell Publishing Ltd 2006 Physiologia plantarum Vol.126 No.4

<P>Genomic research in any organism encompasses understanding structure of the target genome and genes, their function, and evolution. <I>Brassica rapa</I>, which is phylogenetically related to <I>Arabidopsis thaliana</I>, is an important species with respect to its uses as vegetable, oil, and fodder. The availability of suitable genetic and genomic resources is a prerequisite to undertake genomic research in <I>B. rapa</I>. We have developed reference mapping populations of Chinese cabbage (<I>B. rapa</I> ssp. <I>pekinensis</I>) comprising 78 doubled haploid lines and over 250 recombinant inbred lines. Two Bacterial Artificial Chromosome (BAC) libraries, generated by restriction enzymes <I>Hin</I>dIII (KBrH) and <I>Bam</I>HI (KBrB), comprise 56 592 and 50 688 clones, respectively. We have also constructed 22 cDNA libraries from different plant tissues consisting of 104 914 clones with an average length of 575 bp. Initial BAC-end sequence analysis of 1473 clones of the KBrH library led us to understand the structure of <I>B. rapa</I> genome with respect to extent of genic sequences and their annotation, and relative abundance of different types of repetitive DNAs. Full-length sequence analysis of BAC clones revealed extensive triplication of <I>B. rapa</I> DNA segments coupled with variable gene losses within the segments. The formulation of the ‘Multinational Brassica Genome Project’ has laid the foundation to sequence the complete genome of <I>B. rapa</I> ssp. <I>pekinensis</I> by the international Brassica research community. It has been proposed to undertake BAC-to-BAC sequencing of genetically mapped seed BACs. In recent years, development of bioinformatics tools in <I>Brassica</I> has given a boost to structural genomics research in <I>Brassica</I> species. The research undertaken with the availability of various genomic resources in the public domain has added to our understanding of the structure of <I>B. rapa</I>.</P>

Comparative physical mapping of segments of the genomes Brassica oleracea and Brassica campestris that are homoeologous to sequenced regions of chromosomes 4 and 5 of Arabididopsis thaliana

Yong Pyo Lim,Chang Pyo Hong,Jee Young Park,Hyun Su Na,Lan Bancroft 한국육종학회 2001 한국육종학회 학술발표회 발표요지 Vol.2001 No.9

The Reference Genetic Linkage Map for the Multinational Brassica rapa Genome Sequencing Project

Yong Pyo Lim,Su Ryun Choi,Graham R. Teakle,Prikshit Plaha,Jeong Hee Kim,Charlotte J. Allender,Elena Beynon,Zhong Yun. Piao,Tae Ho Han,Graham J. King,Guy C. Barker,Paul Hand Hand,Derek J. Lydiate,Jacqu 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Physical Mapping and Microsynteny of Brassica rapa ssp. pekinensis Genome Corresponding to a 222 kb Gene-Rich Region of Arabidopsis Chromosome 4 and Partially Duplicated on Chromosome 5

Yong Pyo Lim,J.Y. Park,D.H. Koo,C.P. Hong,S.J. Lee,J.W. Jeon,S.H. Lee,P.Y. Yun,B.S. Park,H.R. Kim,J.W. Bang,P. Plaha,I. Bancroft 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Epothilone B inhibits neointimal formation after rat carotid injury through the regulation of cell cycle-related proteins.

Lim, Yong,Kim, Tack-Joong,Jin, Yong-Ri,Kim, Dong-Woon,Kwon, Jin-Sook,Son, Ju-Hee,Jung, Jae-Chul,Avery, Mitchell A,Son, Dong Ju,Hong, Jin Tae,Yun, Yeo-Pyo Williams Wilkins 2007 The Journal of Pharmacology and Experimental Thera Vol.321 No.2

<P>The abnormal proliferation of vascular smooth muscle cells (VSMCs) in arterial walls is an important pathogenetic factor of vascular disorders such as atherosclerosis and restenosis after angioplasty. Epothilone B, a novel potential antitumor compound, has a potent effect on preventing postangioplasty restenosis. Therefore, we established an in vivo rat carotid injury model and examined the potential effects of epothilone B on cardiovascular disease. We found that epothilone B potently prevented neointimal formation and in vivo VSMCs proliferation. In addition, we also showed that epothilone B significantly inhibited 5% fetal bovine serum (FBS)- and 50 ng/ml platelet-derived growth factor (PDGF)-BB-induced proliferation and cell cycle progression in rat aortic VSMCs. Furthermore, FBS and PDGF-BB induced the activations of extracellular signal-regulated kinases 1 and 2, Akt, phospholipase C gamma 1, and PDGF-receptor beta chain tyrosine kinase were not changed by epothilone B. However, epothilone B treatment caused a significant decrease in the level of cyclin-dependent protein kinase (CDK) 2, whereas it caused no change in the levels of cyclin E and down-regulated the phosphorylation of retinoblastoma, which plays a critical role in cell cycle regulation. Furthermore, levels of p27, an inhibitor of cyclin E/CDK2 complex, were significantly increased in VSMCs treated with epothilone B, indicating that this might be a major molecular mechanism for the inhibitory effects of epothilone B on the proliferation and cell cycle of VSMCs. These findings suggest that epothilone B can inhibit neointimal formation via the cell cycle arrest by the regulation of the cell cycle-related proteins in VSMCs.</P>