성상교세포종의 조직학적 등급과 Ag-NORs수, PCNA 발현 및 유세포 측정기를 이용한 DNA 분석의 비교

조미연,정순희,김태승,한용표 대한병리학회 1994 Journal of Pathology and Translational Medicine Vol.28 No.1

신원방우황청심원액의 뇌허혈 및 중추신경계에 대한 약효

조태순,이선미,이은방,조성익,김용기,신대희,박대규 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract-In order to investigate pharmacological properties of New Wonbang Woohwangchungsimwon Liquid(NSCL) and Wonbang Woohwangchungsimwon Liquid(SCL), the effects of NSCL and SCL on cerebral ischemia and central nervous system were compared. Cerebral ischemia insult was performed using unilateral carotid artery occlusion in mongolian gerbils. The histological observations showed a preventive effect of NSCL and SCL treatments with ischemia-induced brain damage. The ATP in brain tissue was decreased in vehicle-treated ischemic gerbils. This decrease was prevented by NSCL and SCL treatment. In contrast to what was seen with ATP, lipid peroxide were elevated in vehicle-treated ischemic gerbils. This elevation was inhibited by NSCL and SCL treatments. While NSCL and SCL had no effects on the hexobarbital-induced sleeping time, they showed sedative effect in rotarod and spontaneous activity test. NSCL and SCL prevented the seizures induced by electric shock and strychnine, but the effect of NSCL was less than that of SCL. Furthermore, NSCL and SCL showed anti-stress effect. Our findings suggest that the phamacological profiles of NSCL on cerebral ischemia and central nervous system are similar to those of SCL.

신원방우황청심원의 뇌허혈 및 중추신경계에 미치는 영향

조태순,이선미,이은방,조성익,김용기,신대희,박대규 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract-In order to investigate pharmacological properties of New Wonbang Woohwangchungsimwon Pill(NSCH) and Wonbang Woohwangchungsimwon Pill (SCH), the effects of NSCH and SCH on cerebral ischemia and central nervous system were compared. Cerebral ischemia insult was performed using unilateral carotid artery occlusion in mongolian gerbils. The histological observations showed preventive effects of NSCH and SCH treatments with ischemia-induced brain damage. The ATP in brain tissue was decreased in vehicle-treated ischemic gerbils. This decrease was prevented by SCH treatment. In contrast to what was seen with ATP, lipid peroxide were elevated in vehicle-treated ischemic gerbils. This elevation was inhibited by NSCH and SCH treatments. While NSCH and SCH had no effects on the hexobarbital-induced sleeping time, they prevented the seizures induced by eletric shock and strychnine. NSCH and SCH showed sedative effect in rotarod and spontaneous activity test. Furthermore, NSCH and SCH showed anti-stress effect. Our findings suggest that the pharmacological profiles of NSCH on cerebral ischemia and central nervous system are similar to those of SCH.

남녀 중학생의 식사 행동과 패스트푸드에 대한 인식 및 이용실태

조정미,한용봉 한국 가정과 교육 학회 1996 한국가정과교육학회지 Vol.8 No.2

This study was to investigated the dietary behavior and fast foods use among Korean youth in order to obtain basic information of nutrition education for them. Survey was carried out through questionnaire and the subjects were 236 boys and 388 girls of 4 middle schools in Seoul. The questionnaire included use frequency of Korean and foreign fast-foods, preference, concepts regarding prices, fullness, choice factors, dietary habits and nutritional Knowledge. The results are summarized as follow : 1. Overweight students(52.8%) were more than normal(43.1% ) and low body weight students (4.2%), classified by Roher index. 2. Moat students(78.9%) had very good or good food habit, 52.6% of boys and 44.0% of girls were having breakfast regulary. 46.1% of subjects had fast-foods once or twice in a week and girl subjects used more than boys. 3. Nutritional knowledge of the subjects was satisfiable and normal and overweight groups were better than underweight group, 4. Fast-foods most students liked were duckbocki(81.1%) fried chicken(80.9%) french fried potatos(80.4%) mandoo(78.1%) kimbab(78.9%), and fried chicken(77.9%). 5. 57.9% of the subjects wished to develope Korean traditional food items into fast-food style.

세균성 뇌막염과 병발된 다엽성이면서 다발성인 화농성 뇌농양 1예

최용석,김정미,한영수,차경만,한정호,조은경,김두응 최신의학사 2004 最新醫學 Vol.47 No.1

데빅병 치료에 사용한 Interferon β-1b의 부작용 1예

김정미,조은경,조정선,최용석,한영수,한정호,김두응,최진영 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

A 63 year-old woman who had diagnosis of multiple sclerosis complaining left hemiparesis and fluctuating decreased visual acuity in her right eye and abnormal findings in pattern shift visual evoked potentials(PSVEPs) and on T2 weighted MR scanning. We treated her subcutaneously with 250μg interferon (3-lb(ITFß -lb) every other day. There were detected pancytopenia, skin rash, and abnormal liver function test seven days after the therapy. In spite of reducing the dose of interferon injection, abnormal laboratory findings were not returned to normal. Dose adjustment of interferon-I3 for body weight or race, may be helpful to reduce the side effects.

위암종과 Epstein-Barr 바이러스와의 연관성에 대한 연구

심광용,김호영,김효열,백순구,권상옥,조미연,이창훈,이종인 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Objectives: Epstein-Barr virus (EBV) has been known to be linked to a spectrum of neoplastic conditions, including nasopharyngeal carcinoma, Burkitt's lymphoma, peripheral T-cell lymphoma and Hodgkin's disease. This study aims to investigate the association of EBV with gastric carcinoma in Korea. Methods: Fifty-three cases of gastric adenocarcinoma were studied for evidence of EBV infection by EBV-encoded small RNA (EBER) in situ hybridization (ISH) on the paraffin sections and amplifying the EBV genome encoding envelop glycoprotein (gp220) with polymerase chain reaction (PCR) in fresh gastric cancer specimens. Results: EBER was detected in 7 (13.2%) of 53 gastric carcinomas and all cases were male. In 6 (85.7%) of 7 EBER-positive cases, the tumors were located in the upper and middle part of the stomach. EBER ISH study showed strong positivity in all the tumor cells, but negativity in surrouning lymphocytes, stromal cells and normal gastric mucosa. DNA PCR was positive in 23 (43.4%) of 53 gastric carcinomas, including all of 7 EBER-positive cases. Conclusion: We could observe some association of EBV with gastric carcinoma, and our findings about the charateristics of EBV-positive gastric cancers involving sex, site and histological type are similar to those in Japan. However, EBV-positive rate of gastric cancer is higher than in Japan and lower than in Western countries. Further studies to elucidate oncogenic mechanism of EBV in gastric cancer should be performed.

흔치않은 대상포진 치험 2예 : 비전형적 부위와 다른 부위로 재발한 예 Unusal Dermatome and Recurs Other Dermatome

김정미,조은경,조정선,최용석,한영수,한정호,김두응,최진영 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Herpes zoster is well known as an acute, self-limited disease of unilateral sensory dermatome caused by varicella-zoster virus and characterized by grouped vesicular skin eruption and pain over several dermatomes as well as single. This virus usually affects the thoracic dermatome and there are a few reports that this virus involves lower lumbar or sacral area, and recurrent cases. We experienced 2 cases that zoster virus involved L4 or S2 dermatome and left typical skin lesions, of which one was a recurrent case involving different dermatome at an interval of 8 years and the other was a rare case involving sacral dermatome.

뇌종양의 악성도 판정을 위한 분열기 세포측정에 관한 연구

한용표,변진수,허철,홍순기,김헌주,조미연,정순희,김호근 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.11

Tissue markers of cellular proliferation have been utilized as prognostic indicators in tumors of the central nervous system. Nucleolar organizer regions represent transcriptionally active sites of ribosomal deoxyribonucleic acid and can be identified by a simple argyrophilic technique. The authors describe a standardized approach to the assessment of these argyrophilic nucleolar organizer regions(AgNORs) in 25 astrocytomas(including 6 glioblastomas), 6 oligodendrogliomas and 1 xanthochromic astrocytoma, which were also estimated in association with 2-year survival post-operatively. Appropriate sections were silver stained and AgNORs were counted in 1000 cells. In cases of astrocytoma, the mean AgNORs/cell count of group survived more than 2 years after operation was significantly different from that survived less than 2 years : 1.93±1.13 vs. ≥ 4.79±3.90. And the mean AgNORs/cell count was 1.53±0.32 in low grade astrocytoma. 2.03±0.59 in grade Ⅲ astrocytoma, and ≥6.64±3.77 in grade Ⅳ astrocytoma. Among the patients with the same grade astrocytoma, the mean AgNORs/cell count was lower in the cases of long survival rather than short survival. The assessment of AgNORs can be easily performed by any surgical pathology laboratory without specialized equipment. It may be used as a valuable and independent prognosticator of biological behavior in cases of astrocytoma.

고혈압치료제의 in vitro및 in vivo 이용률간의 상관성 연구(Ⅱ)

서수경,강현구,조재민,박인숙,안미령,이선우,최흥석,유태무,손수정,이용복,양지선 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약풍이 생체내로 흡수되는_파정에서 생리학적 인자 및 제제학적 인자들에 의해 생체이용률이 달라져 약물의 흡수과정을 연구함으로써 약물의 생체이용률을 예측하는 것이 가능하다. 그러나 제제학적 측면에서 용출시험과 흡수과정인. 장관막 투과도 나아가서 생체이용률간의 띤구가 미흡한 상태이다. 따라서 약물의 흡수과정을 연구하벼 생체이용률을 예측하기 위하여 장관막 투과도 및 혈중농도사이의 상관관계를 예견할 수 있는 모델을 개발하고자 1999년도 연구사업으로 고혈압치료제중 베타-차단약 (아테놀올, 메토프롤올, 프라프라놀꼴)을 선택하여 웅출시험과 장관막 투과도계수를 측정하여 사람의 흡수을을 예측할 수 있는 장관막 투파도 모델을 확림하였고, 2000년도에는 계속사업으로서 각 약물에 대한 횐쥐에서의 생체이용를 파라메타를 측정하고 1999년도 연구사업결과에서 얻은 장관막 투과도 시험결과를 가지고 생체이용률과의 상관섞을 구하고자 하였다. 각 베타-차단약을 경구 및 정맥투여하고 시간별로 채혈한 후 분리한 혈장으로부터 각 약물에 대한 EfLC를 이용한 분석준건을 확지하여 혈중농도를 측정하고 생체이용률 파라메타(AUC, Cma, Tme, 등)를 구하였다. 횐쥐의 소장을 이용하여 diffusion chamber 법으로 측정한 장관막 투과도계수와 각 생체이용률 파라미터 사이의 상관성을 구찬 결과 양호한 상관관계를 보였다. 이와 같은 연구결과를 통하여 약물의 특성에 따라 동물의 장관막 투도. 모델을 이용하여 사람의 생체이용률도 예측할 수 있을 것으로 사료된다. A biopharmaceutics drug classification system for correlation f'H uf'fro dissolution and rH ui'ㄴo bio3vailabi3ity is proposed based on recognizing that drug dissolution andgastrointestinal permeability are the fundamental parameters controlling rate and extent of drugabsorption. The objective of this stud)· was to assess whether partition coefficient and intestinalPermeabilit)'can be rorrelateo with l'H vluo bioalFaifability. Beta-blockers were administeredorall? and intrauenousl)· to rats and pkarrnacokinetlc parameters were calculated. The goodcorre)ations between fermeability and log P (n~octanol/buffer) was demonstrated in previous ratexpenments performed in our laboratory. In this study, good correlations were obseFYedbetuTeen panitlon coefficient and permeabilit)·· or I'n ui'vo bioayilability These resuats indiEatethat rat intestinal perme3bilit3T model couBd be used to predict intestinal absorption ofbeta-blockeTs f'H uiuo.