폐광지역 토양과 농작물 중의 중금속 오염도 조사

방은옥,정용준,조영채 忠南大學校 環境問題硏究所 2002 環境硏究 Vol.20 No.-

This study was conducted to investigate the concentration of the heavy metals among the soils and crops, and the difference compared with the unpolluted area from the radius distance from the ruined mine. Gubong Mind in Cheongyang-Gun, Chungnam-Do was selected as the ruined mine, which the Taechi-Myon district was selected ad the unpolluted control area. Pepper, welch onion, cabbage, radish and their soils were collected at the mine and unpolluted area, respectively. The concentration of heavy metals(Pb, Cd, Cu, Zn and As) were measured by ICP. In the soil sample, the average concentrations of all the heavy metals were significantly increased in the min area compared with those of the unpolluted area and significantly differed from the radius distance from ruined mine. In the crop sample, the average concentration of Pb, Zn and As metals were significantly increased in the mine area compared with those of the unpolluted area and all the heavy metals were significantly differed from the radius distance from ruined mine. There was a significant negative correlation among the soils from the radius distance from ruined mine and among the crops in the concentrations of all the heavy metals. There was a significant positive correlation between soils and crops in concentration of Pb, Cd, Zn and As metals. The results indicate the concentrations of heavy metals among the soils and crops at the ruined mine were saturated more than the unpolluted and the soil metals had spread to the crops. Consequently, we cannot leave the matter unsettled and its utilization must be studied to lower the contamination degree.

개선된 구조의 3.3V CMOS 전류모드 적분기 설계

방준호,오성근,김동용 全北大學校 1995 論文集 Vol.40 No.-

In this paper, a CMOS current-mode integrator adequate to design low-voltage, low-power and high frequency application circuit. The designed current-mode integrator has a new architecture that is simple. When the designed integrator is compared with typical current-mode integrator, the new integrator has lower power dissipation, lower chip size and higher frequency range. The results of PSPICE simulation using ORBIT company's 1.2㎛ CMOS n-well processing parameter shows that 1.2mW power dissipation for 3.3V power supply.

다종재료용 RP 시스템을 위한 3D 슬라이서 구현

공용해,엄태준,방재철,김기석 순천향대학교 부설 산업기술연구소 2001 순천향 산업기술연구소논문집 Vol.7 No.1

We developed an algorithm that effectively extracts slicing points from STL 3D models for a new RP system which can fabricate various materials. Since an STL file has a simple data structure and contains much embiguity, we considered all the cases between facet positions and slicing planes for effective slice extraction from the STL file, and reduced the overall processing time. The effectiveness and efficiency of the proposed slicing method was tested by many STL 3D samples.

CGI 프로그램에서 클라이언트와 서버의 자동화된 상태정보 전달

최준용,유진호,장종표,방만원 木浦大學校 情報産業硏究所 1997 情報産業硏究誌 Vol.5 No.-

The HTTP Protocol composed of WWW's client/server model is unconnected and not keep state, so, there is one connection for one request. In this condition, it has no guarantee to memory which is needed in sequential processing, and HTTP Protocol has no choices to transfer the information from client to server's CGI or the information exchange. This paper uses the word 'key' instead of this 'state information'. We studied the key which creates automatically the HTML document for data search hierarchically in WWW environment and to confirm applied this to multimedia order and sale system of agricultural and marine products.

고주파 능동필터를 위한 CMOS 트랜스컨덕터 설계

金東龍,方駿鎬 全北大學校 1995 論文集 Vol.39 No.-

In this paper, a CMOS transconductor adequate to design high frequency active filter is designed. The designed transconductor has wide input linearity, high output impedance and tuning capability for minimizing characteristics variation after integrating. The results of PSPICE simulation using ORBIT company's 2㎛ CMOS n-well processing parameter shows that a ±3V input linear range, 300MHz cut-off frequency at -3dB, 8mW power dissipation for ±5V power supply.

대구지방 소아 및 토양에서 분리한 Microsporum gypseum의 교배형에 대한 연구

노병의,방용준 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.1

Microsporum gypseum complex의 교배형 및 감염원을 조사하기 위하여 대구에 거주하는 소아에서 분리한 M-gypseum 17주와 환자주변의 초등학교 운동장, 화단 및 어린이 놀이터의 토양에서 분리한 60주를 표준균주 A-gypsea 및 A.incurvata와 교배 실험을 실시 하였다. 소아에서 분리된 M.gypseum 17주의 성별 완전형의 분포는 남아에서 A.incurvata가 14주 이었고, A.gypsea는 3주 이었다. 여아에서는 A.incurvata만 4주 분리 되었다. 병형별 분리빈도는 Tinea facial가 A.gypsea 2주, A.incurvata 10주로 다른부위 보다 많이 분리 되었다. 교배형의 분포는 환자에서 분리한 17주중 A.gypsea는 3주중 "+"형 2주, "-"형이 1주 이었고, A.incurvata는 "+"형이 6주, "-"형이 8주 이었다. 토양에서 분리한 60주의 교배형의 분포는 A.gypsea는 31주중 "+"형 16주, "-"형이 15주 이었고, A.incurvata는 "+"형이 15주, "-"형이 14주 이었고 특히 소아에서 많이 분리된 A.incurvata가 어린이 놀이터에서 많이 분리된 것을 보면 소아의 M.gypseum 감염은 놀이터와 관련성이 있을 것으로 사료 된다. A study was conducted to evaluate isolation rate and distribution of mating type of M.gypseum complex Arthroderma(A.) gypsea and A.incurvata. Seventeen(17) strains were isolated from children patients and sixty(60) strains were from elementary school playgrounds, flower beds, and children,s playgrounds in Taegu area. The strains were crossed with test strains of A.gypsea and A.incurvata which were provided by Tokyo university. The results were summarized as follows; Ofseventeen(17) strains, thirteen(13) were isolated from male children and ten(10) were A.incurvata and three(3) A.gypsea, and four(4) were from female children and all four strains were A.incurvata. Face(Tinea facial) was more frequently affected to compare with other clinical type. Of seventeen(17) strains isolated from patients, six(6) were A.incurvata "+" type and eight(8) were "-" type, and two(2) were A.gypsea "+" type and one(1) was "-" type. Of sixty(60) strains isolated from soil, sixteen (16) were A.gypsea "+" type and fifteen(15) were"-" type. and fifteen(15) were A.incurvata "+" type and fourteen(14) were "-" type.

전류모드방식 적분기의 구조개선을 통한 성능비교

이근호,조성익,방준호,김동용 全北大學校 論文集 Vol.42 No.-

In this paper, a CMOS fully-differential complementary current-mode integrator adequate to design low-voltage, low-power and high frequency application circuit. The designed fully-differential complementary current-mode integrator has a new architecture. When the improved integrator is compared with proposed current-mode integrator, the new integrator has lower power dissipation and higher frequency range. The small signal analysis results are showed in table.1 with several improved characteristics. And the results of PSPICE simulation using ORBIT company's 1.2㎛ CMOS n-well processing parameter shows fig. 3.

웹 기반 멀티미디어 농수산물 주문판매 시스템

서성채,최준용,방만원 木浦大學校 情報産業硏究所 1997 情報産業硏究誌 Vol.5 No.-

The high-speed communication backbone has the features that it is faster than any previous communication media, larger in the bandwidth and lower in the error rates. For cut-edge technical development and various service in the high-speed communication backbone, it is firstly required the technique that the multimedia informations such as texts, voices, pictures and videos are processed on real-time and then they are utilized through the connections. The future shopping and ordering system will be required as the dynamic and interactive system which can directly identify the process, size and shape of products and order, not as the static which have only texts data. And it will arise the need for the efficient management of multimedia DB. In this paper, We developed the multimedia order and sale system of agricultural and marine products. We expect that it enables to the home-shopping that users can search the informations of agricultural and marine products in home or office, and order the production after identifying with pictures and videos.

Pocine Adenovirus-3의 E1B Region의 鹽基序列 分析

朴鍾賢,宋載永,李重馥,玄芳勳,安東濬,車相昊,裵用泰,姜永源,Reddy, P S,全茂炯,安壽煥 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

돼지 아데노바이러스(PAV-3). 6618주의 EIB region이 包含되어 있는 map unit 4.0에서 9.7까지의 유전자에 대한 1,984 bp의 염기서열을 決定하였으며, 이 結果를 알려진 여러 아데노바이러스 유전자와 비교하여 다음과 같은 결과를 얻었다. 1. PAV-3의 EIB유전자는 10개의 ORF로 구성되어 있으며, 그 중 아데노바이러스의 단백질과 유사성이 있는 것은 ORF1, ORF2 및 ORF3이었다. ORF1은 Ad41의 19kd 와 BAV-2에서의 EIB ORF2에서의 아미노산의 一致率은 각각 32%와 31%이었다. 2. ORF2는 Ad2 55kd protein과 tupaia adenovirus 44kd protein가 각각 34%로 아미노산 一致率이 가장 높았으며, Ad41의 52kd protein. BAV-3의 EIB ORF3에서도 33%의 一致率을 보였다. 3. ORF1은 61-666 uncleotide (606 bp), ORF 2에서는 429-1,850 uncleotide (1,422 bp)의 부위로 각각 202, 474 a.a로 構成되었으며, 예상되는 분자량은 20 kd와 52 kd이었다. 4. ORF3는 hexon-associated pIX유전자로 추정되며 내부에 1개의 polyadenylation signal(ATAAA)이 1938-1942 uncleotide에 위치하였으며, 이 부위는 TATA box (1937-1942 uncleotide)와 중복되어 존재하였다. Porcine adenovirus type 3 (PAV-3) does not cause severe infection in pigs. Adenovirus has been suggestive of live vaccine vector carrying foreign gene. One of insertion regions is delayed early (EIB) region. However, EIB region of PAV-3 has not been molecularly characterized to date. Nucleotide sequence of EIB of PAV-3 was determined. The EIB region was composed of 1,984 bp and located between 4.0 and 9.7 map units. Three potential open reading frames(ORFs) with low level of homology to other adenoviruses and a polyadenylation signal were identified in the rightward direction of genome. The nucleotide and the predicted amino acid sequences of EIB were compared to those of human and animal adenoviruses. One of the three potential ORFs. ORF1 encoded a polypeptide homologous to bovine adenovirus type 2(BAV-2) ORF2 and human adenovirus type 41(Ad41) 19 kd protein. ORF2 encoded a polypeptide homologous to human adenovirus type 2(Ad2) 55 kd protein, bovine adenovirus type 3(BAV-3) ORF3 and porcine adenovirus type 4(PAV-4) ORF2. The predicted protein of ORF1 had homology to those of Ad41 and BAV-2 with 32 and 31% respectively, whereas the deduced protein of ORF2 had homology to those of Ad2. BAV-3 and PAV-4 with 34, 33 and 29%, respectively.

[P278] A case of Fonsecaea monophora skin infection

( Jun Hong Park ),( Dong Hyuk Eun ),( Sun Young Moon ),( Jun Young Kim ),( Yong Hyun Jang ),( Seok-jong Lee ),( Do Won Kim ),( Yong Jun Bang ),( Jae Bok Jun ),( Weon Ju Lee ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.1

Chromoblastomycosis is a chronic fungal infection of the cutaneous and subcutaneous tissue resulting from implantation of certain dematiaceous fungi through the skin. The genus Fonsecaea is a major dematiaceous fungus and accounts for 90% of chromoblastomycosis. The genus Fonsecaea has been reclassified using analysis of rDNA internal transcribed spacer (ITS) region sequence; Fonsecaea (F.) pedrosoi, F. monophora, and others. In Korea, F. monophora chromoblastomycosis is very uncommon and just 4 cases have been reported. F. monophora infection was confirmed using rDNA ITS sequence analysis. Additionally characteristic sclerotic cells on both KOH preparation and histopathologiy can be helpful in making a diagnosis. A 65-year-old immunocompetent male presented with a walnut-sized, scaly erythematous plaque on left forearm for 1 year. KOH preparation showed brown sclerotic bodies and fungal culture showed dark black, velvety colonies. Long, septate hyphae with numerous conidia were observed on lactophenol cotton blue staining. Histopathology demonstrated brownish sclerotic bodies and suppurative granulomatous infiltration in the dermis. Also, sequencing analysis of the ITS region of rDNA in GenBank identified F. monophora. The lesion was successfully controlled by oral itraconazole (100mg bid for 2 months). We report a case of F. monophora chromoblastomycosis which is very rare in Korea, identified with gene sequencing analysis.