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Cui, Sheng-Yun,Kim, Seung-Jin,Jo, Sung-Chan,Lee, Yong-Moon,Lee, Yong-Ill Korean Chemical Society 2005 Bulletin of the Korean Chemical Society Vol.26 No.8
A combination of electrospray ionization and tandem mass spectrometry was used to characterize the scavenging reactions of acrylamide (AA) in the presence of glutathione (GSH) in vitro. In the presence of GSH, AA was deactivated effectively and scavenged by reactions consuming small amount of GSH. Reaction products and structural information were identified using collision-induced dissociation (CID) in an ion trap mass spectrometer. In the mixture of GSH and AA, significant increase in abundance of fragment ion peak was observed at m/z 233, which was identified as $[Cys-Glu]^+$, formed by the elimination of glycine moiety of GSH. GSH also contributes to the AA scavenging reaction by conjugating with AA through the sulfhydryl group in cysteine moiety. The probable scavenging reaction pathway of AA in the presence of GSH has been proposed based on the CID experimental data.
식용 해조류 톳(Hizikia fusiformis) 유래 항산화 활성분획물의 산화적 손상이 유도된 인간피부섬유아세포 보호 효과
Yong Ri Cui,김현수,제준건,Lei Wang,오재영,Liu Jia,전유진 한국수산과학회 2019 한국수산과학회지 Vol.52 No.1
We investigated the protective effects of antioxidant fractions from a 70% ethanolic extract of Hizikia fusiformis in human dermal fibroblasts (HDFs). Powdered H. fusiformis was extracted with 70% ethanol and then partitioned into three fractions according to polarity using n-hexane (HFH), chloroform (HFC), and ethyl acetate (HFEA). Antioxidant activity was observed in HFEA at 0.66 mg/mL based on the half maximal inhibitory concentration (IC50) of 1,1-diphenyl-2-picrylhydrazyl (DPPH), and at 0.24 mg/mL based on alkyl radical scavenging. The protective effects of the HFEA antioxidant fraction against 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH)-damaged HDFs and the expression of Type I procollagen in HDFs were examined. HFEA caused the proliferation of HDFs with and without AAPH treatment and protected against AAPH damage to HDFs in a dose-dependent manner (50-200 μg/mL). This implies that the antioxidant properties of the fractions depended on their proliferative and protective effects. The HFEA antioxidant fraction had significant effects and caused the dose-dependent expression of Type I procollagen, an important anti-wrinkle protein, in HDFs. In conclusion, antioxidant substances in H. fusiformis were found in the ethyl acetate fraction, and the resulting HFEA may have cosmetic applications.
Yong-Ill Lee,Seung-Jin Kim,Sung-Chan Jo,Yong-Moon Lee,Shengyun Cui 대한화학회 2005 Bulletin of the Korean Chemical Society Vol.26 No.8
A combination of electrospray ionization and tandem mass spectrometry was used to characterize the scavenging reactions of acrylamide (AA) in the presence of glutathione (GSH) in vitro. In the presence of GSH, AA was deactivated effectively and scavenged by reactions consuming small amount of GSH. Reaction products and structural information were identified using collision-induced dissociation (CID) in an ion trap mass spectrometer. In the mixture of GSH and AA, significant increase in abundance of fragment ion peak was observed at m/z 233, which was identified as [Cys-Glu]+, formed by the elimination of glycine moiety of GSH. GSH also contributes to the AA scavenging reaction by conjugating with AA through the sulfhydryl group in cysteine moiety. The probable scavenging reaction pathway of AA in the presence of GSH has been proposed based on the CID experimental data.
Yong Cui,Yong-Bo Song,Lin Ma,Yan-Feng Liu,Guo-Dong Li,Chun-Fu Wu,Jing-Hai Zhang 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.10
This study utilized the E. coli expression system to investigate the role of amino acid residues in toxin from the Chinese scorpion - Buthus martensii Karsch (BmKAS). To evaluate the extent to which residues of the toxin core contribute to its analgesic activity, ten mutants of BmKAS were obtained by PCR. Using site-directed mutagenesis, all of these residues were substituted with different amino acids. This study represents a thorough mapping and elucidation of the epitopes that form the molecular basis of the toxin’s analgesic activity. Our results showed large mutant-dependent differences that emphasize the important roles of the studied residues.
A bond graph approach to energy efficiency analysis of a self-powered wireless pressure sensor
Cui, Yong,Gao, Robert X.,Yang, Dengfeng,Kazmer, David O. Techno-Press 2007 Smart Structures and Systems, An International Jou Vol.3 No.1
The energy efficiency of a self-powered wireless sensing system for pressure monitoring in injection molding is analyzed using Bond graph models. The sensing system, located within the mold cavity, consists of an energy converter, an energy modulator, and a ultrasonic signal transmitter. Pressure variation in the mold cavity is extracted by the energy converter and transmitted through the mold steel to a signal receiver located outside of the mold, in the form of ultrasound pulse trains. Through Bond graph models, the energy efficiency of the sensing system is characterized as a function of the configuration of a piezoceramic stack within the energy converter, the pulsing cycle of the energy modulator, and the thicknesses of the various layers that make up the ultrasonic signal transmitter. The obtained energy models are subsequently utilized to identify the minimum level of signal intensity required to ensure successful detection of the ultrasound pulse trains by the signal receiver. The Bond graph models established have shown to be useful in optimizing the design of the various constituent components within the sensing system to achieve high energy conversion efficiency under a compact size, which are critical to successful embedment within the mold structure.
Yong-Nan Xu,Sang-Jun Uhm,Bon-Chul Koo,Mo-Sun Kwon,Ji-Yeol Roh,Jung-Seok Yang,Hyun-Yong Choi,Young-Tae Heo,Xiang-Shun Cui,Joon-Ho Yoon,Dae-Hwan Ko,Teoan Kim,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2013 Reproductive & Developmental Biology(Supplement) Vol.37 No.2s
The potential benefits of generating and using transgenic cattle range from im-provements in agriculture to the production of large quantities of pharmaceutically rele-vant proteins. Previous studies have attempted to produce transgenic cattle and other livestock by pronuclear injection and somatic cell nuclear transfer, but these approa-ches have been largely ineffective; however, a third approach, lentivirus-mediated trans-genesis, has successfully produced transgenic livestock. In this study, we generated transgenic (TG) Korean native cattle using perivitelline space injection of viral vectors, which expressed enhanced green fluorescent protein (EGFP) systemically. Two different types of lentiviral vectors derived from feline immunodeficiency virus (FIV) and human immunodeficiency virus (HIV) carrying EGFP were injected into the perivitelline space of MII oocytes. EGFP expression at 8-cell stage was significantly higher in the FIV group compared to the HIV group (47.5±2.2% vs. 22.9±2.9%). Eight- cell embryos that expressed EGFP were cultured into blastocysts and then transferred into 40 heifers. Ten heifers were successfully impregnated and delivered 10 healthy calves. All of these calves expressed EGFP as detected by in vivo imaging, PCR and Southern blotting. In addition, we established an EGFP-expressing cell line from TG calves, which was followed by nuclear transfer (NT). Recloned 8-cell embryos also expressed EGFP, and there were no differences in the rates of fusion, cleavage and development between cells derived from TG- and non-TG calves, which were subsequently used for NT. These results illustrate that FIV-based lentiviruses are useful for the production of TG cattle. Moreover, our established EGFP cell line can be used for additional studies that involve induced pluripotent stem cells.
Effects of combined BCG and DHEA treatment in preventing the development of asthma.
Cui, Yong,Choi, Inseon S,Koh, Young-Ah,Lin, Xiang-Hua,Cho, Yong-Bum,Won, Young-Ho Marcel Dekker 2008 Immunological investigations Vol.37 No.3
<P>Both BCG and dehydroepiandrosterone (DHEA) induce Th1 immune responses and suppress Th2 allergic reactions. To investigate whether the combination of BCG and DHEA has an additive effect on asthma prevention, BALB/c mice (n = 10 per group) were given an intraperitoneal injection of BCG at the beginning of sensitization, and fed mice chow containing DHEA throughout the study. In female mice, the combined administration of 2 x 10(4) CFUs BCG and 0.01% DHEA effectively suppressed the ovalbumin-induced increase in airway sensitivity to methacholine (56.5 vs. 8.2 mg/mL, p < 0.01), while BCG (13.9 mg/mL) or DHEA (17.9 mg/mL) alone did not. However, the addition of high dose (0.1%) DHEA decreased the efficacy of high dose (2 x 10(5) CFUs) BCG in suppressing the airway responsiveness and eosinophilia. In male mice, the treatments with BCG and/or DHEA were less effective, and the interferon-gamma/interleukin-4 ratio in the splenocyte supernatant was significantly higher and the ovalbumin-specific IgE concentration in the serum was significantly lower as compared to female mice. In conclusion, the combination of low doses of BCG and DHEA had an additive effect in suppressing the development of airway hypersensitivity. Androgens in males and DHEA overdose might reduce the efficacy of BCG.</P>