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Moon, Phil-Dong,Han, Na-Ra,Ko, Seong-Gum,Jeong, Hyun-Ja,Kim, Su-Jin,Lee, Bo-Ra,Kim, Na-Hyung,Choi, In-Young,An, Hyo-Jin,Myung, Noh-Yil,Lee, Hyejung,Kim, Myong-Jo,Kim, Ju-Sung,Hong, Seung-Heon,Kim, Hyu Butterworths [etc.] 2010 Neurological research Vol.32 No.suppl1
<P>OBJECTIVES: Dokhwaljihwang-tang, one of the prescriptions in Four Constitution medicine, has been used to treat neurological disorders. This work was aimed to examine the effect of Dokhwaljihwang-tang on lipopolysaccharide-induced inflammatory cytokine production in peripheral blood mononuclear cells from the lesser yang subjects. METHODS: The inflammatory cytokines, interleukin 1 beta, interleukin 4 and tumor necrosis factor were measured using the ELISA. The interleukin 1 beta mRNA expression was evaluated with reverse transcriptase-polymerase chain reaction. The expression level of nuclear factor kappa B was examined by western blotting. RESULTS: Dokhwaljihwang-tang significantly inhibited interleukin 1 beta and interleukin 4 production (p<0.05). When Dokhwaljihwang-tang was pre-treated at the dose of 1 mg/ml, it not only decreased the interleukin 1 beta mRNA expression, but also inhibited the activation of nuclear factor kappa B. DISCUSSION: These results suggest the potential of Dokhwaljihwang-tang as a source of pharmaceutical acupuncture for neuronal inflammatory diseases.</P>
韓國에 있어서 Longidoridae科 線虫의 分類學的 硏究 1 : 한국산 Longidoridae 科의 3 미기록종에 관하여
Young Eoun Choi,Yil Seong Moon 한국응용곤충학회 1988 한국응용곤충학회지 Vol.27 No.3
한국산 Longidoridae 과 선충을 채집하여 분류동정한 결과 경상북도에서 Xiphinema속의 X. zulu, X. setariae 그리고 Longidorus속의 L. sylphus등 3종이 우리나라 미기록종으로 밝혀졌으며 이들의 형태적 특징, 채집지, 기주식물 등을 보고한다. Taxonomical study on the Longidoridae from korea were undertaken at Kyungsangbuk-do. Three species belomging to two Longidoridae were identified. Xiphinema zulu, X. setatiae and Longidoridae sylphus were newly reported from Korea.
Jung-Ok Kong,Sang-Myung Lee,Yil-Seong Moon,Sang-Gil Lee,Young-Joon Ahn 한국응용곤충학회 2006 Journal of Asia-Pacific Entomology Vol.9 No.2
The nematicidal activity and poisoning symptoms of 88 plant essential oils against Bursaphelenchus xylophilus were examined by an immersion bioassay. Results were compared with those of three trunk-injection nematicides: fenitrithion, levamisol hydrochloride, and morantel tartrate. As judged by 24 h LC50 values, cinnamon bark oil (0.12 mg/ml) was the most effective nematicide, followed by coriander herb oil (0.14 mg/ml). Potent nematicidal activity was also observed with lemongrass, oregano, thyme red, and clove bud oils (LC50, 0.57-0.88 mg/ ml). Fenitrothion was ineffective (LC50, >10 mg/ml). In typical poisoning symptoms in B. xylophilus, these essential oils exerted rapid nematicidal action and the nematodes killed usually showed an extended shape, whereas levamisole hydrochloride and morantel tartrate usually exhibited semicircular and coiling shapes, respectively. The essential oils described merit further study as botanical nematicides for the control of pine wilt disease caused by B. xylophilus.
Kong, Jeong-Ok,Lee, Sang-Myung,Moon, Yil-Seong,Lee, Sang-Gil,Ahn, Young-Joon SOCIETY OF NEMATOLOGISTS 2007 JOURNAL OF NEMATOLOGY Vol.39 No.1
<P>The nematicidal activity of two cassia, Cinnamomum cassia, oils (Especial and true), four cinnamon, Cinnamomum zey-lanicum, oils (technical, #500, bark and green leaf), and their compounds (e.g., trans-cinnamaldehyde and trans-cinnamic acid) toward adult Bursaphelenchus xylophilus was examined by a direct contact bioassay. Results were compared with those of 34 related compounds. As judged by 24-hour LC(50) values, two cassia oils (0.084-0.085 mg/ml) and four cinnamon oils (0.064-0.113 mg/ml) were toxic toward adult B. xylophilus. Of 45 test compounds, trans-cinnamaldehyde (0.061 mg/ml) was the most active nematicide, followed by ethyl cinnamate, alpha-methyl-trans-cinnamaldehyde, methyl cinnamate and allyl cinnamate (0.114-0.195 mg/ml). Potent nematicidal activity was also observed with 4-methoxycinnamonitrile, trans-4-methoxycinnamaldehyde, trans-2-methoxy-cinnamaldehyde, ethyl alpha-cyanocinnamate, cinnamonitrile and cinnamyl bromide (0.224-0.502 mg/ml). Structure-activity relationships indicate that structural characteristics, such as types of functional groups, saturation and carbon skeleton, appear to play a role in determining the toxicities to adult B. xylophilus. Cassia and cinnamon oils and test compounds described merit further study as potential nematicides or leads for the control of pine wilt disease caused by B. xylophilus.</P>
Kong Jung-Ok,Lee Sang-Myung,Moon Yil-Seong,Lee Sang-Gil,Ahn Young-Joon Korean Society of Applied Entomology 2006 Journal of Asia-Pacific Entomology Vol.9 No.2
The nematicidal activity and poisoning symptoms of 88 plant essential oils against Bursaphelenchus xylophilus were examined by an immersion bioassay. Results were compared with those of three trunk-injection nematicides: fenitrithion, levamisol hydrochloride, and morantel tartrate. As judged by 24 h $LC_{50}$ values, cinnamon bark oil (0.12 mg/ml) was the most effective nematicide, followed by coriander herb oil (0.14 mglml). Potent nematicidal activity was also observed with lemongrass, oregano, thyme red, and clove bud oils ($LC_{50}$, 0.57-0.88 mg/ml). Fenitrothion was ineffective ($LC_{50}$, >10 mg/ml). In typical poisoning symptoms in B. xylophilus, these essential oils exerted rapid nematicidal action and the nematodes killed usually showed an extended shape, whereas levamisole hydrochloride and morantel tartrate usually exhibited semicircular and coiling shapes, respectively. The essential oils described merit further study as botanical nematicides for the control of pine wilt disease caused by B. xylophilus.
Kwan-Soo Woo,Yeong-Sik Kim,Yeong-Bon Koo,Jin-Kie Yeo,Yil-Seong Moon 한국식물병리학회 2007 Plant Pathology Journal Vol.23 No.2
We conducted an inoculation test using nine openpollinated families of pine trees to evaluate their susceptibility and mortality in different densities of pine wood nematode. Three-year-old nine open-pollinated pine families were inoculated with Bursaphelenchus xylophilus at levels of 3,000, 5,000, and 7,000 nematodes/ seedling in greenhouse. There were no distinct patterns in latent period among three densities of B. xylophilus in all families. Most families showed the first disease symptoms of needle discoloration within 12-15 days after inoculation. However, open-pollinated progenies of Pinus densiflora showed the longest latent period because none of one-year-old needles were wilted until 14 days after inoculation with 5,000 and 7,000 nematodes. One-year-old needles were wilted earlier than current needles in all tested families with all densities of B. xylophilus. Current needles were not wilted until 14 days after inoculation in all seedlings. The mortality of all seedlings rapidly increased from 35 days to 49 days after inoculation, and all died within 80 days except two seedlings. A 3,000 nematodes/100 μL with sterilized distilled water are enough to screen 3- year-old pine seedlings for resistance to B. xylophilus.
솔수염하늘소(Monochamus alternatus) 훈증방제를 위한 수종 살충제의 살충활성 및 훈증조건
이상명 ( Sang Myeong Lee ),정영진 ( Yeong Jin Chung ),문일성 ( Yil Seong Moon ),이상길 ( Sang Gil Lee ),이동운 ( Dong Woon Lee ),추호렬 ( Ho Yul Choo ),이총규 ( Chong Kyu Lee ) 한국산림과학회 2003 한국산림과학회지 Vol.92 No.3
Fumigation effect of metam-sodium 25% SL, aluminium phosphide GA, bifenthrin 4% FW, diasinon 3% G, and Armoracia rusticana was evaluated against larvae of Japanese pine sawyer(Monochamus alternatus) at the damage areas of Pine wilt disease by Bursaphelenchus xylophilus for one rear from April, 2001 to April, 2002. In addition, application time of metam-sodium and fumigation effect depending on the height of piled timber and mulching of soil surface were investigated at the Piled timber. Effect of rate of metam sodium was also evaluated at the stump of cut black pine (Pinus thunbergii). Mortality of Japanese pine sawyer larvae was 18% at the rate of aluminium phosphide GA 8 tablets/ m^3, 40% at that of 12 tablets/ m^3, and 100% at that of 42 tablets/m^3, respectively. Metam sodium 25% WP was effective showing 98% mortality at the rate of 0.5ℓ/m^3 and the rate of metam sodium inducing 100% mortality was over 0.7ℓ/m^3. However, bifenthrin 4% FW and diazinon 3% GR were not effective against larvae of Japanese pine sawyer. On the contrary 100% mortality was shown at the rate of 60㎖/m^3 of Armoracia rusticana oil. Application time of metam sodium did not affect mortality of Japanese pine sawyer larvae, i.e., mortality was 100% in 7 days after treatment in March, May, October, and December. Metam sodium killed 100% of Japanese pine sawyer larvae in cut timber by 1 m in the fumigation tent regardless of height. Effect of mulching of soil surface for 48 h influenced efficacy of metam sodium and A. rusticana oil. Metam sodium killed 100% of Japanese pine sawyer larvae without relation to mulching. However, A. rusticana oil did not kill Japanese Pine sawyer larvae in the non mulching area while 100% mortality was shown in the mulching area.