http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
새조개 가공부산물을 이용한 속성 액젓의 제조 및 저장안정성
김명희,조영철,배태진,손정호,김귀식 여수대학교 1997 論文集 Vol.11 No.2
A rapid processing for fermented fish sauce from cockle shell by-product and their quality and storage stability was investigated. The weight ratio of cockle shell by-product about the whole cockle shell was 32.7%, and contents of moisture, portein, lipid, carbohydrate and ash were 83.1%, 10.7%, 2.4%, 1.0% and 2.9%, respetively. The cockle shell by-product was homogenized with addition of water and hydrolyzed at 50℃ for 8 hours added 4% Protease N.P.(3.14ㆍ10⁴U/g). The hydrolysate was thermally treated at 100℃ for 60 minutes with 4% invert sugar and 20% table salt was finally added to the product. The chemical compositon of moisture, carbohydrate, ash, salinity, total nitrogen and amino type nitrogen of fermented sauce from cockle shell by-product were 65.4%, 3.9%, 20.8%, 18.9%, 1,683 mg% and 1,426 mg%, respectively. The major nucleotides and their relative compounds in the product were AMP and hypoxanthine and their contents were 3.36μ mole/g and 1.95μ mole/g. And major free amino acid were glutamic acid, lysine, leucine, valine and aspatic acid.
정상 성인에서 총콜레스테롤의 분별치와 관련 요인 : 전북지역 한 종합병원의 정기검진자를 중심으로
김지식,여영규,홍인선 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.2
정상 성인에서 혈청 총 콜레스테롤의 분별치 및 관련된 제요인을 파악하기 위하여 1995년 3월부터 10월까지 8개월 동안 전주 예수병원에서 의료보험 피보험자와 피부양자를 대상으로한 정기 건강 검진을 받은 대상자 중 성별, 연령별, 혈압, 체중, 기타 생화학적 검사 결과를 분석하였으며, 이들 중 고혈압 등 질병이 없는 건강한 집단으로 1,818명을 분석하였다. 혈청 총 콜레스테롤의 분별치를 산정 하기 위한 75th percentile값을 보면, 21∼30세군에 191 mg/dl, 31∼40군에서 195 mg/dl, 41∼50세군에서 214 mg/dl, 51∼60세군에서 227 mg/dl, 그리고 61세 이상 군에서 222 mg/dl이었다. 90th percentile값을 보면, 21∼30세군에서 214 mg/dl, 31∼40군에서 214 mg/dl, 41∼50세군에서 239 mg/dl, 51∼60세군에서 253mg/dl, 그리고 61세 이상군에서 248 mg/dl 이었다. 혈청 총 콜레스테롤과 각 변수간의 상관관계는 연령과는 남.여 모두 유의한 관계를 보였고(p<0.001), 특히 여자에서 높은 상관성을 보였다. 비만지수인 PIBW, BMI와도 유의한 상관성을 보였고, 수축기혈압과 이완기혈압과도 관련성을 보였다. 대상자들의 흡연 여부와 혈청 총 콜레스테롤과의 관계는 흡연군이 191.6±35.0 mg/dl 이었고, 비흡연군이 194.9±35.9 mg/dl였으며 두 집단간에는 유의한 차이를 보이지 않았다. 음주에 따른 혈청 총 콜레스테롤의 측정치는 비음주군에서는 196.0±36.0 mg/dl 이었고, 음주군에서는 188.9±34.5 mg/dl로 두 군간에는 유의한 차이를 보였다. 운동 습관과 혈청 총 콜레스테롤의 관계는 규칙적으로 운동을 하는 군은 192.1±33.3 mg/dl이었고, 운동을 하지 않는 군은 195.8±37.0 mg/dl였으며 두 군간에는 유의한 차이를 보였다. The cut-off values and related factors for total cholesterol of serum in normal Korean adults were studied from March to October, 1995, based on the data of 1,818 healthy adults of human involved the male and female. The comparisons of the serum total cholesterol levels were analyzed by sex, age, systolic blood pressure, diastolic blood pressure, body weight, height, PIBW, BMI and other biochemical tests. 1. The serum total cholesterol level were 194.4 mg/dl in total group, 186.9 mg/dl for male, and 197.6 mg/dl for female. 2. There was no significant differences by sex among the 21 to 50 ages group, but significantly higher in women than in men over 51 years old. 3. In general, the levels of serum total cholesterol were increased with age, the highest level in men was 192.7 mg/dl in 51 to 60 years old, and 210.5 mg/dl in more than 60 years old in women. 4. The cut-off values of serum total cholesterol for moderate risk were 191 mg/dl in 21 to 30 years group, 195 mg/dl in 31 to 40 years, 214 mg/dl in 41 to 50 years, 227 mg/dl in 51 to 60 years, and 222 mg/dl in more than 61 years. The cut-off values of serum total cholesterol for high risk were 214 mg/dl in 21 to 30 years group, 214 mg/dl in 31 to 40 years, 239 mg/dl in 41 to 50 years, 253 mg/dl in 51 to 60 years, and 248 mg/dl in more than 61 years. 5. The mean values of serum total cholesterol were showed positive correlation with age, PIBW, BMI, systolic blood pressure and diastolic blood pressure. 6. Serum total cholesterol level of drinking group(188.9±34.5mg/dl) was showed lower than those of non-drinking group(196.0±36.0mg/dl). 7. Serum total cholesterol levels of regular excercise group(192. 1±33.3 mg/dl) was significantly lower than those of non regular excercise group(195.8± 37.0 mg/dl).
Kim, Seon Yeong,Park, Hong-Gyu,Cho, Min-Jae,Jeong, Hae-Chang,Kim, Dai-Hyun,Seo, Dae-Shik The Electrochemical Society 2014 ECS journal of solid state science and technology Vol.3 No.11
<P>Liquid crystal (LC) alignment on various HfZrO films as a function of Hf concentration was studied. In the HfZrO system, Hf can act as a supporter to produce a uniform alignment layer. From pre-tilt measurement, LC cells with 5% and 10% Hf were optimized to perform as a device. By X-ray photoelectron spectroscopy (XPS) analysis, the proper amount of Hf addition in the ZrO system resulted in less oxygen vacancies which caused the LCs to be orientated more uniformly. However, too much Hf led to degradation of the LC cells due to the formation of HfO<SUB>2</SUB> in the HfZrO films.</P>
Purification and Characterization of β - Galactosidase from Green Onion
Yeong Shik Kim,Kyung Shin Park,Jong Goon Kim 생화학분자생물학회 1993 BMB Reports Vol.26 No.7
β-D-Galactosidase from the mature tissues of green onion was purified to homogeneity using a procedure involving gel-filtration, anion-exchange chromatography, hydroxyapatite chromatography, and affinity chromatography on p-aminobenzyl-l-thio-β-D-galactopyranoside-agarose. Its molecular weight was estimated to be 52,000 Da and its isoelectric point was 6.5. The overall yield was 0.2% and specific activity was increased approximately 380 folds. Activity was maximum at pH 4 and 50℃. The purified enzyme was demonstrated to be specific to Gal-β1-6 linkage using fluorescence-labeled substrates.
Kim, Yeong-Shik,Hahn, Bum-Soo,Cho, So-Yean,Chang, Il-Moo The Korean Society of Toxicology Korea Environment 2001 Toxicological Research Vol.17 No.-
Three types of proteases (MEF-1, MEF-2 and MEF-3) were purified from the egg cases of Ten-odera sinensis using ammonium sulfate fractionation, gel filtration on Bio-Gel P-60 and affinity chromatography on DEAE Affi-Gel blue gel. The proteases were assessed homogeneous by SDS-polyacrylamide gel electrophoresis and have molecular weight of 31,500, 32,900 and 35,600 Da, respectively. The N-terminal regions of the primary structure were compared and they were found to be different each other. MEFs readily digested the $A\alpha$ - and B$\beta$-chains of fibrinogen and more slowly the ${\gamma}$-chain. The action of the enzymes resulted in extensive hydrolysis of fibrinogen and fibrin, releasing a variety of fibrinopeptides. MEF-1 was inactivated by Cu$^{2+}$ and Zn$^{2+}$ and inhibited by PMSF and chymostatin. MEF-2 was inhibited by PMSF, TLCK. soybean trypsin inhibitor. MEF-3 was only inhibited by PMSF and chymostatin. Antiplasmin was not sensitive to MEF-1 but antithrombin III inhibited the enzymatic activity qf MEF-1. MEF-2 specifically bound to anti plasmin Among the chromogenic protease substrates, the most sensitive one to the hydrolysis of MEFs was benzoyl-Phe-Val-Arg-p-nitroanilide with maximal activity at pH 7.0 and 3$0^{\circ}C$. MEF-1 preferentially cleaved the oxidized B-chain of insulin between Leu15 and Tyr16. In contrast, MEF-2 specifically cleaved the peptide bond between Arg23 and Gly24. D-dimer concentrations increased on incubation of cross-linked fibrin with MEF-1, indicating the enzyme has a strong fibrinolytic activity.ity.
Glycosaminoglycan lyases and acharan sulfate
Yeong Shik Kim 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1
Glycosaminoglycans (GAGs) are unbranched, polydisperse, acidic polysaccharides, often covalently linked to a protein core to form proteoglycans (PGs). The most common GAGs are heparin, heparan sulfate (HS), hyaluronic acid (HA), chondroitin sulfate (CS), dermatan sulfate (DS), and keratan sulfate (KS). Heparin, an anticoagulant isolated from animal tissue, is an important and chemically unique polysaccharide of considerable biological significance. In order to understand the complicated structure of GAGs, GAG lyases from microorganisms have been widely used and their reaction products were analyzed using various HPLC methods. We have cloned and expressed GAG lyases of Bacteroides thetaitaomicron in E.coli. In collaboration with Dr Mirek Cygler’s group, the tertiary structures of recombinant chondroitinases ABC and heparinase have been solved. These things will be presented. Acharan sulfate (AS), isolated from the giant African snail Achatina fulica, primarily consists of the repeating disaccharide structure a-D-N-acetylglucosaminyl (1→4) 2-sulfoiduronic acid. Its structure is related to heparin and heparan sulfate but is distinctly different from all known members of these classes of glycosaminoglycans. Because of its structural similarities to heparin, a diverse array of biological activities and functions have been studied. Because of its high molecular weight and copper binding affinity, specific proteins in the plasma could be purified by affinity chromatography.