초등과학 탐구수업 지도자료의 활용 실태

신영준,장명덕,배진호,권난주,여상인,이희순,노석구 한국초등과학교육학회 2005 초등과학교육 Vol.24 No.2

In this study, we had tried to present a plan for improving the actual conditions of practical utilization of teaching material for 6th grade science developed by Ministry of Education & Human Resources Development and distributed to all elementary schools in Korea. Also we presented ways of better utilization of the teaching material after investigating the actual conditions of practical utilization. A survey was made to investigate and collect all data in the major metropolitan cities, the Kangwon, Chungcheong, Honam, and Kyeongsang area, respectively. We surveyed 316 6th grader teachers to investigate the actual conditions of distribution and utilization of teaching material as a general research. In addition, we surveyed 46 teachers to investigate the organization and content of teaching material as a particular research. The results are as follows. First, the teaching material was not approximately transmitted and kept to 6th grader teachers. Second, the utilization guide was not made. Third, it was reported that the thematic divisions of teaching material was a strong point, but the less detailed experiment manual was a weak point. Fourth, the consideration of content difficulties and simplicity was necessary to improve the material. Fifth, additional items should be included in the introductory presentation, convenience of reorganization, activity material causing learning interest, guidance of substitution experiment, and more concrete notice of experiment activity. Finally, there were positive responses of more than 4.0 point of Likert scale (1 to 5 point scale) in detail investigations of thematic items, which could have possibility that the teaching material was helpful to elementary school science field.

아동기 가정 내 스트레스가 공동의존성에 미치는 영향 및 공동의존성과 정신병리와의 관련성

곽운환,손지욱,한규희,박철수,김장락,김붕년,김봉조,강여화,하형래 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.1

연구목적: 본 연구는 공동의존성에 영향을 미치는 아동기 가정내 스트레스 요인과 공동의존성과 다른 병리와의 연관성을 규명하고자 하였다. 더불어 특정한 질환에서의 공동의존성의 평가가 가지는 의의를 탐색해 보고자 하였다. 방 법: 연구 대상은 정상 일반인 남성집단 88명과 알코올리즘 남성 집단 76명으로 구성되어 있다. 평가도구는 본 저자가 직접 작성한 '아동기 가정내 스트레스 요인 측정용 설문'과 공동의존 척도(RPS), 간이증상 진단검사(SCL-90-R)를 사용하였다. 통계적 분석은 Mann-Whitmey test, Kruskall- Wallis test, Multiple linear regression analysis, Spearman correlation analysis를 이용하였다. 결 과: 1) 정상 일반인 군과 알코올리즘 군의 공동의존성 평균값은 각각 49.12점(표준편차=15.41)과 88.31점(표준편차=17.48)이었다. 2) 정상 일반인 군에서는 '아동기 부모 사별'(p<0.01) '부모의 알코올리즘'(p<0.01) '부모의 상습적 구타'(p<0.01)의 3가지 요인에서, 알코올리즘 군에서는 '어린시절 부모 사별'(p<0.01) '부모의 알코올리즘'(p<0.01) '부모의 상습적 구타'(p<0.01) '부도의 도박'(p<0.01) '사회 병질자 부모의 존재'(p<0.05)의 5가지 요인에서 스트레스 유무에 따라 공동의존성에 유의한 차이를 보였다. 3) 두 집단 동시에 SCR-90-R의 8개 소척도 중 '대인관계 예민성'이 공동의존성과 가장 높은 연관성(rs>0.06, p<0.01)을 보였다. 이외에도 '우울증' '불안증'등이 두 집단 모두에서 통계적으로 유의한 연관성을 보였다. 결 론: 본 연구는 부모의 알코올리즘뿐만 아니라 여러 가정 내 스트레스 요인이 공동의존성의 발생에 영향을 미칠 수 있음과 공동의존성이 높은 집단이 다른 정신과 질환을 동반할 가능성이 높음을 증명한다고 할 수 있다. Objectives : The aim of this study was to examine the relationships of codependency with family stressors in childhood and psychopathology. Methods : Subjects were a group of 88 normal adult males and a group of 76 alcoholic males. The degree of codependency was assessed with the Korean version of Recovery Potential Survey(RPS). All subjects filled out questionnaires regarding demographic information and family stressors. The psychopathology was measured by self-administred questionnaires using Symptom Check List-90-Revision(SCL-90-R). Results : 1) There were no significant differences in codependency levels by sociodemographic characteristics in two groups, but obvious difference in mean of codependency scores(normal group : 49.12±15.41, alcoholism group : 88.31±17.48). 2) There were significant differences in codependency levels between the presence and absence of parental physical abuse, parental death, and parental alcoholism(p<0.01) in both groups. 3) Among family stressors, parental physical abuse had the largest contribution to codependency level in multiple linear regression. 4) There were significant correlations between the codependency levels and the scores of SCL-90-R's subscales of interpersonal sensitivity, depression, and anxiety in both groups. Conclusion : The codependency levels were related to some family stressors such as parental physical abuse, parental death, and parental alcoholism and the high-level codependency was correlated with higher level of psychopathology in both normal and alcoholism group. These results suggested that various family stressors, as well as parental alcoholism may be predictors of codependency, and codependency also may be a predictor of other psychiatric disorders.

참돌꽃 근경의 항산화작용

류광열,강원식,김영호,장해동,홍진태,유환수,윤여표 충남대학교 약학대학 의약품개발연구소 1998 藥學論文集 Vol.14 No.-

The purpose of this study was to determine the antioxidative effects of Rhodiola sachalinensis Rhodiola methanol extract was fractionated sequentially with dichloromethane and butanol. Each Rhodiola fraction (water, MeOH, BuOH and CH_2Cl_2 fractions) showed the potent superoxide dismutase (SOD) activity, and had inhibitory effets on peroxide value of linoleic acid (40~57%) and lipid peroxidation (47~70%) in FE^2+/ascorbate system-induced rat liver microsome. Rhodiola methanol extract also recovered carbon tetrachloride-induced decrease in SOD by 42% and catalase activities by 50%, and had inhibitory effects (54%) on carbon tetrachloride-induced lipid peroxidation in rat liver microsome. These results suggest that Rhodiola sachalinensis has the antioxidative effects.

Biliary Complication during ECMO Treatment

( Jin Ook Jang ),( Hye Ju Yeo ),( Woohyun Cho ),( Eunjeong Son ),( Jinho Jang ),( Tae Hwa Kim ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.0

Background Extracorporeal membrane oxygenation (ECMO) has wide range of complications. Among GI tract complications, biliary problems such as bile stasis or acute acalculous cholecystitis are not fully assessed. Given cardiopulmonary instability, this serious subset of patients presents difficult challenges in adequate diagnosis and timely treatment of biliary complication. However, little is known about the incidence and clinical significance of biliary complications in patients on ECMO. We aimed to evaluate the incidence, associated factors and clinical outcomes of biliary complication during ECMO. Methods We retrospectively analyzed and reviewed ECMO cohort group (n=549) of our center from 2008.12 - 2019.10 and excluded 82 patients with less than 48hrs of ECMO application or having statistically missing data. ECMO cohort group (n=549) included entire ECMO cases of our center. We defined biliary complication as newly appeared radiologically diagnosed biliary related disease during ECMO, more specifically complicated biliary problems as biliary disease with inflammation such as cholecystitis, pancreatitis and uncomplicated biliary disease as rather incidental finding such as GB stones, GB sludge. Results 52 cases of total (n=467) had biliary complication. The incidence is 11.13% of total cases. Statistically (table 1), old age (P = 0.003), NPO days (P = 0.04), TPN days (P=0.003), and Presence of Hyperbilirubinemia (P = 0.026) have meaningful correlation with biliary complication during ECMO. Gender, BMI, SOFA, APACHE II, Renal replacement therapy, inotropic use, presence of liver cirrhosis, diuretic use and total bilirubin did not differ between two groups. Especially, plasma Hb, indicative mechanical hemolysis during ECMO, also did not differ between ECMO having biliary complication and without biliary complication. (P = 0.996) Conclusions Biliary complication was associated with old age, longer duration of fasting, days on total parenteral nutrition and the presence of hyperbilirubinemia. The predictors of biliary complications did not differ from those of the general ICU population.

The Effect of Acori Graminei Rhizoma Pharmacopuncture at GV20 on Dementia in a Focal Cerebral Ischemia Mice Model

Jang, Yeo jin,Kwak, Min Kyung,Jeong, Sang Jun,Kim, Hye Hwa,Kim, Tae Gwang,Kim, Jae Hong Korean AcupunctureMoxibustion Medicine Society 2017 대한침구의학회지 Vol.34 No.3

Objectives : The purpose of this study was to examine the effects of Acori Graminie Rhizoma Pharmacopuncture (PA-AG) at GV20 on cerebral ischemia-induced dementia in Mice. Methods : Mice were divided into the five following groups: normal, control, acupuncture, PA-AG (17 mg/kg), and PA-AG (34 mg/kg). All groups, except the normal group, had cerebral ischemia induced by occlusion of middle cerebral artery. The control group was not treated. The acupuncture, PA-AG (17 mg/kg), and PA-GA (34 mg/kg) groups were treated every other day with a total of 6 treatments. The effect of treatment was observed by Bax, Bcl-2, Bax/Bcl-2 ratio, cytochrome c, cresyl violet, and choline acetyltransferase staining. Results : In the PA-AG (34 mg/kg) group, the intensity of Bax was decreased and the intensity of Bcl-2 was increased. The Bax/Bcl-2 ratio also decreased in the PA-AG (34 mg/kg) group. The intensity of cytochrome c protein stain was decreased in the PA-AG (17 mg/kg) group. The density of neurons stained by cresyl violet and choline acetyltransferase (ChAT) was increased in the AT, PA-AG (17 mg/kg), and PA-AG (34 mg/kg) groups when compared with that of the control group. Conclusion : PA-AG at GV20 was effective on cerebral ischemia-induced dementia in mice.

Poster Presentation : Influence of Smoking Habits on the DNA Damage, Endothelial Function and Lipid Peroxidation in Healthy Men

( Yeo Jin Lim ),( Soo Jeong Koh ),( Sun Hee Park ),( Yae Jung Hyun ),( Hyae Jin Kim ),( Bo Ram Cha ),( Jong Ho Lee ),( Yang Soo Jang ) 한국지질동맥경화학회(구 한국지질학회) 2003 춘계학술대회 Vol.- No.-

Development and Validation of Simultaneous Analytical Method for Pesticides in Cnidium officinale using GC-NPD

Yeo Jin Jang(장여진),Hyun Young Park(박현영),Da Eun Lee(이다은),Byung Hee Lee(이병희),Kyu Yeob Kim(김규엽),Chul Hyun Lee(이철현),Jwa Haeng Park(박좌행),Soo Yeul Cho(조수열) 한국약용작물학회 2022 한국약용작물학회 학술대회논문집 Vol.2022 No.1

The Biomarkers for Prediction of Significant Bleeding Complication during ECMO Support

( Jin Ho Jang ),( Hye Ju Yeo ),( Woo Hyun Cho ),( Taehwa Kim ),( Yun Seong Kim ),( Doosoo Jeon ),( Jin Ook Jang ),( Eunjeong Son ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.0

Background Bleeding is the main complication of extracorporeal membrane oxygenation (ECMO) support. Currently, the mechanism of bleeding during ECMO is not fully understood. In previous studies, we have shown that ECMO has an effect of reducing platelet activation in the early stages. This preliminary study aimed to find a biomarker and clinical parameters for predicting bleeding complications. Methods We prospectively collected blood samples from ECMO patients on day 1, 3 and 7 and analyzed their plasma beta-thromboglobulin (beta-TG), platelet factor 4 (PF4), tumor necrosis factor alpha (TNF-α), and interleukin 1 beta (IL-1b). We retrospectively reviewed patient medical records and analyzed correlations between platelet function and inflammatory markers and bleeding. Results Overall, 54 patients who received ECMO for acute respiratory failure were included and classified by presence of bleeding complication (the bleeding group (n=11) and the non-bleeding group (n=45)). The mean TNF-α concentration on day 1 (p=0.026) and day 3 (p=0.018) were significantly different between two groups, respectively. Among the clinical parameters, ECMO application for more than 2 weeks (p=0.002) were significantly related to bleeding complication (p=0.002). The ROC curve revealed that the mean TNF-α concentration on day 1 and day 3 after ECMO application fairly discriminated bleeding group and non-bleeding group with an AUC of 0.734 (95% CI 0.60-0.84, p=0.001) and 0.778 (95% CI 0.65-0.88, P<0.001), respectively. However, the factors associated with platelet activity did not show any significant difference between the two groups (Fig. 2). Conclusion The early serum level of TNF-α may play a role as a significant biomarker of bleeding complication during ECMO. The marker of platelet activity, such as beta-TG and PF4 were not significant predictor of bleeding during ECMO support. Further research aiming the crosstalk of TNF-α and coagulation cascades is warranted.

Paraquat Induces Apoptosis through a Mitochondria-Dependent Pathway in RAW264.7 Cells

Jang, Yeo Jin,Won, Jong Hoon,Back, Moon Jung,Fu, Zhicheng,Jang, Ji Min,Ha, Hae Chan,Hong, SeungBeom,Chang, Minsun,Kim, Dae Kyong The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.5

Paraquat dichloride (N,N-dimethyl-4-4'-bipiridinium, PQ) is an extremely toxic chemical that is widely used in herbicides. PQ generates reactive oxygen species (ROS) and causes multiple organ failure. In particular, PQ has been reported to be an immunotoxic agrochemical compound. PQ was shown to decrease the number of macrophages in rats and suppress monocyte phagocytic activity in mice. However, the effect of PQ on macrophage cell viability remains unclear. In this study, we evaluated the cytotoxic effect of PQ on the mouse macrophage cell line, RAW264.7 and its possible mechanism of action. RAW264.7 cells were treated with PQ (0, 75, and $150{\mu}M$), and cellular apoptosis, mitochondrial membrane potential (MMP), and intracellular ROS levels were determined. Morphological changes to the cell nucleus and cellular apoptosis were also evaluated by DAPI and Annexin V staining, respectively. In this study, PQ induced apoptotic cell death by dose-dependently decreasing MMP. Additionally, PQ increased the cleaved form of caspase-3, an apoptotic marker. In conclusion, PQ induces apoptosis in RAW264.7 cells through a ROS-mediated mitochondrial pathway. Thus, our study improves our knowledge of PQ-induced toxicity, and may give us a greater understanding of how PQ affects the immune system.

YC-1 Induces S Cell Cycle Arrest and Apoptosis by Activating Checkpoint Kinases

Yeo, Eun-Jin,Ryu, Ji-Hye,Chun, Yang-Sook,Cho, Young-Suk,Jang, In-Jin,Cho, HoSung,Kim, Jinho,Kim, Myung-Suk,Park, Jong-Wan American Association for Cancer Research 2006 Cancer Research Vol.66 No.12

<P>Hypoxia-inducible factor-1alpha (HIF-1alpha) seems central to tumor growth and progression because it up-regulates genes essential for angiogenesis and the hypoxic adaptation of cancer cells, which is why HIF-1alpha inhibition is viewed as a cancer therapy strategy. Paradoxically, HIF-1alpha also leads to cell cycle arrest or the apoptosis of cancer cells. Thus, the possibility cannot be ruled out that HIF-1alpha inhibitors unlock cell cycle arrest under hypoxic conditions and prevent cell death, which would limit the anticancer effect of HIF-1alpha inhibitors. Previously, we reported on the development of YC-1 as an anticancer agent that inhibits HIF-1alpha. In the present study, we evaluated the effects of YC-1 on hypoxia-induced cell cycle arrest and cell death. It was found that YC-1 does not reverse the antiproliferative effect of hypoxia, but rather that it induces S-phase arrest and apoptosis at therapeutic concentrations that inhibit HIF-1alpha and tumor growth; however, YC-1 did not stimulate cyclic guanosine 3',5'-monophosphate production in this concentration range. It was also found that YC-1 activates the checkpoint kinase-mediated intra-S-phase checkpoint, independently of ataxia-telangiectasia mutated kinase or ataxia-telangiectasia mutated and Rad3-related kinase. These results imply that YC-1 does not promote the regrowth of hypoxic tumors because of its cell cycle arrest effect. Furthermore, YC-1 may induce the combined anticancer effects of HIF-1alpha inhibition and cell growth inhibition.</P>