New therapeutic method by non-thermal atmospheric pressure bio-compatible plasma (NBP) for tissue engineering

Ye Jin Choi,Eun Ha Choi,Ihn Han 한국진공학회 2020 한국진공학회 학술발표회초록집 Vol.2020 No.2

Diagnostic Neuroimaging in Headache Patients: A Systematic Review and Meta-Analysis

Ye Eun Jang,Eun Young Cho,Hee Yea Choi,Sun Mi Kim,Hye Youn Park 대한신경정신의학회 2019 PSYCHIATRY INVESTIGATION Vol.16 No.6

Objective Neuroimaging in headache patients identifies clinically significant neurological abnormalities and plays an important role in excluding secondary headache diagnoses. We performed a systematic review and meta-analysis of the existing guidelines and studies surrounding neuroimaging in headache patients. Methods The research question involved determining the prevalence of detecting clinically significant neurological abnormalities using neuroimaging in patients suspected of primary headache. Searches of the PubMed and Embase databases were conducted on English- language studies published from 1991 to 2016, and the reference lists of the retrieved articles were also checked manually. All headache subtypes and patients aged ≥15 years were included in the analysis. Results Ten studies met the selection criteria. The pooled prevalence of detecting clinically significant abnormalities in the neuroimaging of headache patients was 8.86% (95% confidence interval: 5.12–15.33%). Subsequently, diverse subgroup analyses were performed based on the detection method, headache type, study type, study region, age group, and disease type. Conclusion The present findings indicate that limited neuroimaging methods should be carefully considered for headache diagnostic purposes when there are red flag symptoms. Limitations and suggested directions for future studies on neuroimaging in headache patients are described.

Fungal Distribution in Traditional Meju and Characterization of Isolated Strains

Ye-Eun Son,Ye-Jin Kang,Sun-Young Choi,Yoon-Kyung Choi,Ju-Eun Lee,Junyoung Kim,박희수 한국균학회 2023 韓國菌學會誌 Vol.51 No.3

This study was conducted to analyze the distribution and characteristics of fungal speciesin meju using the traditional method. Fungal distribution in meju was investigated usingmetagenomic and morphological analyses, based on which Aspergillus flavus/oryzae strainswere identified as the dominant fungi in all meju samples, followed by Pichia, Rhizopusand Lichtheimia spp. As A. flavus/oryzae was dominant, we further evaluated the aflatoxinproduction ability and enzymatic activity of the isolates. Thin-layer chromatography andpolymerase chain reaction revealed that the A. flavus/oryzae strains isolated from meju arenon-aflatoxigenic fungi. Based on the analyses of amylase and protease activities, strainswith high activities of amylase or protease were identified, which are proposed to be used asstarters for meju fermentation.

Antioxidant and anti-obesity effect through redox mechanism of A. scaber ethanolic extract

Ye Eun Choi,Sun il Choi,Xionggao Han,Xiao Men,Gill Woong Jang,Hee-Yeon Kwon,Ok-Hwan Lee 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

Expressions of microRNAs related to third cleavage event in mouse blastocysts and its conditioned media

Ye Eun Lee,Jihyun Kim,Yoon Ji Choi,Jung Eun Park,Seok Hyun Kim,Jin Hyun Jun 한국발생생물학회 2017 한국발생생물학회 학술발표대회 Vol.2017 No.8

Spatiotemporal expressions of microRNAs (miRNAs) are altered by the physiological states of cells which could be influenced by microenvironment. Function of miRNAs has been focused as a new regulator of gene expressions and cell differentiation in human health and diseases. We found and identified the several miRNAs, which were related to developmental competence of preimplantation and implantation process of mouse blastocysts and outgrowth embryos by microarray-based bioinformatical studies. In this study, we evaluated three miRNAs expressions related to third cleavage event in conditioned media (CM) and blastocysts. Mouse 2-cell stage embryos were collected and monitored for 9 hours. The embryos were divided two groups as early third cleavage before 9 hours of collection and late third cleavage after 9 hours of collection. They were cultured to blastocyst stage up to day-5 after hCG injection. The total number of cells and the number of cells with fragmented DNA were assessed in blastocysts by terminal dUTP nick-end labelling (TUNEL) staining and DAPI staining. Mean cell number of early third cleavage group was significantly higher than that of late third cleavage group (105.3±8.0 vs 81.8±7.0, p<0.05), but apoptotic index was not different. The miRNAs of CM and blastocysts from early and late group were prepared, and quantified by qRT-PCR with TaqMan probes. The expression levels of three miRNAs (mmu-let-7b, mmu-miR-183, and mmu-miR-429) in CM and blastocysts were slightly upregulated in late third cleavage group. Our study suggested that the expression level of miRNAs could be altered with embryo quality, and miRNAs in CM may be used to predict miRNAs expression of embryos and developmental competence.

Nanoencapsulation of Combined Antimicrobial Compounds for Improving Oral Health

Ye-Seul Choi,Eun Sung Cho,Eun Suh Kim,Ji-Soo Lee,Hyeon Gyu Lee 한국식생활문화학회 2017 한국식생활문화학회학술대회논문집 Vol.2017 No.11

SARS-CoV-2-Naïve Korean Children and Adolescents Hospitalized With COVID-19 in 2021

Choi Youn Young,Choi Soo-Han,Choi Jae Hong,Kim Dong Hyun,Lee Joon Kee,Eun Byung Wook,Lee Hyunju,Kim Ye Kyung,Ahn Bin,Song Seung Ha,Yun Ki Wook 대한의학회 2022 Journal of Korean medical science Vol.37 No.42

Background: The risk of severe outcomes with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) delta variant remains low in children and adolescents, but less is known about its effect on the SARS-CoV-2-naïve population. This study evaluated clinical manifestations and risk factors for moderate-to-critical coronavirus disease 2019 (COVID-19) in mostly SARS-CoV-2-naïve children and adolescents in 2021. Methods: This multicenter retrospective study included patients aged 0–18 years who were hospitalized with COVID-19 at 8 referring hospitals in South Korea during the predeltapredominant and delta-predominant periods in 2021. Each case was labeled as either hospitalization with medical needs or for isolation. Severity was categorized as mild, moderate, severe, or critical with regard to pneumonia presence and illness severity. Results: Among 753 cases, most (99.5%) had no prior history of COVID-19 or vaccination against COVID-19. The proportions of hospitalization with medical needs (3.5% vs. 19.7%), moderate illness (0.9% vs. 4.0%), and severe/critical illness (0.8% vs. 5.3%) increased during delta predominance. The risk of moderate-to-critical COVID-19 among hospitalizations with medical needs was higher among patients aged 12–18 years (adjusted odds ratio [aOR], 4.1; 95% confidence interval [CI], 1.5–11.8) and with obesity (aOR, 6.9; 95% CI, 2.4–19.6) but not among patients infected during delta predominance. However, children with obesity experienced more severe COVID-19 during delta predominance (aOR, 6.1; 95% CI, 1.2–29.6). Conclusion: Despite its similar severity among most SARS-CoV-2-naïve children and adolescents, the delta variant may affect COVID-19 severity in those with high-risk underlying medical conditions. Underlying conditions, particularly obesity, may cause severe COVID-19 in children and adolescents, warranting strong consideration for vaccinating high-risk children.

The Coiled-coil Domain of a Subgroup of Plant NLR Immune Receptor Induces Cell Death

Ye-Eun Seo,Hye-Young Lee,Ha-Eun Kim,Eunsook Park,Hyung-Gon Mang,Doil Choi 한국원예학회 2019 한국원예학회 학술발표요지 Vol.2019 No.5

기하학적 모델링을 위한 조작적, 사회적, 급진적 구성주의의 비교와 다차원적 적용 방법

최예은(Ye-eun Choi),서대룡(Dae-ryong Seo) 인문사회과학기술융합학회 2018 예술인문사회융합멀티미디어논문지 Vol.8 No.3

본 연구는 구성주의를 통해 나올 수 있는 수치들을 창의적인 기하학적 모델링으로 나타내는데 목 적이 있다. 조작적 구성주의, 사회적 구성주의, 급진적 구성주의라는 3가지 유형의 구성주의는 같은 뿌리로부터 시작되었지만 그 안에서 강조하는 부분은 각각 다르다. 이러한 강조점과 차이점에 따라 3 가지 유형의 구성주의를 분류하였고, 평가 기준 표를 제작하였다. 또한, 폴수학학교 웹사이트 상에서 보이는 설립 철학, 교육 목표, 교육과정 특징, 비교과교육과정, 전체교육과정의 내용들을 구문 분석하 여 3가지 구성주의의 평가 기준 표에 적용시켰다. 적용된 부분으로부터 수치를 얻어 낼 수가 있었으 며, 이 수치를 Excel 프로그램에 입력하여 다양한 그래프로 표현하였다. 본 연구를 통해 얻어진 데이 터 수치와 그래프 상의 표현을 통하여, 폴수학학교 웹사이트의 내용들이 3가지 구성주의와 얼마나 관 련이 있는지를 알 수가 있었다. 구성주의의 3가지 유형 각각에서 나오는 5가지의 특징들과 폴수학학 교 웹사이트 5개 영역의 조합으로부터 전체 75개의 표본을 얻어낼 수 있었고, 평가 기준표 위에서 폴 수학학교 웹사이트 5가지 영역의 내용에서 추출한 평균들이 통계적으로 차이가 있는지를 판단하기 위해서 귀무가설과 대립가설을 세웠다. 일원분산분석이라는 통계적 기법을 사용하였으며, 분석 결과 유의확률이 0.015로 0.05보다 작으므로 대립가설을 채택하게 되어 평균값이 최소한 한 개는 다르다는 결론을 얻었다. 그리고 좀 더 세밀한 통계적인 분석을 위하여 기술통계량, 등분산 검정, 개체·간 효과 검정에 뒤이어서 사후 분석을 실시하였고, 그 결과 폴수학학교 비교육과정이 교육과정보다 구성주의 성향이 더 강하다는 것을 알 수 있었다. 마지막으로는 총합한 데이터들을 x, y, z라는 3가지 변수로 대응시켰고 Wolfram Mahematica 10.4 프로그램으로 시각화하여 베지어 곡선으로 나타내었다. The purpose of this research is to show the numerical values of constructivism through creative geometrical modeling. Three types of constructivism are operational constructivism, social constructivism, and radical constructivism which are originated from the same root. However, they have different main points. This research classified three types of constructivism according to main points and differences, and an evaluation criteria table was created. In addition, contents of the founding philosophy, educational goals, curriculum features, extra curriculum, and the whole curriculum of the Paul Math school web site are analyzed and applied to the evaluation rubric table related to three types of constructivism. The numerical value obtained was input into the Excel program and was expressed on a variety of graphs. 75 samples from 5 different features from each of the three types of constructivism and from five areas of Paul Math school website were collected. A null hypothesis and an alternative hypothesis were set up in order to compare averages of five 5 areas of Paul Math school web site. One-way ANOVA was used for this test. As a result of the analysis, the significance probability is 0.015, which is less than 0.05. Therefore, the alternative hypothesis was accepted and the researcher concluded that at least one of averages is different. For more detailed statistical analysis, post-hoc analysis as well as descriptive statistics, levene’s test for the equality of variances, and test of between-subjects effects were conducted. As a result, it was found that constructivist trend of extra curriculum of Paul Math school was stronger than that of core curriculum. Finally, the researcher applied the data to Wolfram Mathematica 10.4 program and expressed geometric objects in Bezier curves.

Identification and Functional Characterization of Two Noncoding RNAs Transcribed from Putative Active Enhancers in Hepatocellular Carcinoma

Lee, Ye-Eun,Lee, Jiyeon,Lee, Yong Sun,Jang, Jiyoung Joan,Woo, Hyeonju,Choi, Hae In,Chai, Young Gyu,Kim, Tae-Kyung,Kim, TaeSoo,Kim, Lark Kyun,Choi, Sun Shim Korean Society for Molecular and Cellular Biology 2021 Molecules and cells Vol.44 No.9

Enhancers have been conventionally perceived as cis-acting elements that provide binding sites for trans-acting factors. However, recent studies have shown that enhancers are transcribed and that these transcripts, called enhancer RNAs (eRNAs), have a regulatory function. Here, we identified putative eRNAs by profiling and determining the overlap between noncoding RNA expression loci and eRNA-associated histone marks such as H3K27ac and H3K4me1 in hepatocellular carcinoma (HCC) cell lines. Of the 132 HCC-derived noncoding RNAs, 74 overlapped with the eRNA loci defined by the FANTOM consortium, and 65 were located in the proximal regions of genes differentially expressed between normal and tumor tissues in TCGA dataset. Interestingly, knockdown of two selected putative eRNAs, THUMPD3-AS1 and LINC01572, led to downregulation of their target mRNAs and to a reduction in the proliferation and migration of HCC cells. Additionally, the expression of these two noncoding RNAs and target mRNAs was elevated in tumor samples in the TCGA dataset, and high expression was associated with poor survival of patients. Collectively, our study suggests that noncoding RNAs such as THUMPD3-AS1 and LINC01572 (i.e., putative eRNAs) can promote the transcription of genes involved in cell proliferation and differentiation and that the dysregulation of these noncoding RNAs can cause cancers such as HCC.