Preparation of Bioactive Poly(Lactic-Co-Glycolic)Acid and Silica Gel Fibers Mixed Non-Woven Fabric

Seol, Yang Jo,Kim, In Ae,Lee, Yong Keun,Lim, Bum Soon,Rhee, Sang Hoon Trans Tech Publications, Ltd. 2007 Key Engineering Materials Vol.361 No.-

<P>Poly(lactic-co-glycolic)acid and silica gel fibers mixed non-woven fabric was made by electro-spinning method for the potential application as a bone grafting material. The silica gel, the source material for electro-spinning, was prepared by the hydrolysis of tetraethyl orthosilicate in the presence of calcium salt, water, hydrochloric acid and ethanol. Poly(lactic-co-glycolic)acid solution was prepared by dissolving it in the hexafluoroisopropanol. Then, they were transferred to two separate syringes which were connected to the high voltage supply generating a high electric field between the spinneret and the ground collecting drum. The silica gel containing calcium and poly(lactic-co-glycolic)acid solution were spun together under the electric field of 2 ㎸/㎝. The FE-SEM observations showed that the silica gel and poly(lactic-co-glycolic)acid fibers were mixed together completely and its handling property was much improved compared to that of the non-woven silica gel fabric. After soaking in the SBF for 1 week, low crystalline apatite crystals were also observed to occur on the silica fiber surfaces first and then they were also observed to occur on the poly(lactic-co-glycolic)acid fiber surfaces. From the results, it can be concluded that the poly(lactic-co-glycolic)acid and silica gel fibers mixed non-woven fabric made by electro-spinning method has a bioactivity. It means it has a potential to be used as a bone grafting material because of its apatite-forming ability, high surface area to volume ratio and high porosity.</P>

Bioactivity, pre-osteoblastic cell responses, and osteoconductivity evaluations of the electrospun non-woven SiO₂–CaO gel fabrics

Seol, Yang-Jo,Kim, Kyoung-Hwa,Kang, Young Mi,Kim, In Ae,Rhee, Sang-Hoon Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of biomedical materials research. Part B, Vol.b90 No.2

<P>The evaluations of the fibers characteristics, bioactivity, pre-osteoblastic cell responses, and osteoconductivity of the non-woven SiO<SUB>2</SUB>–CaO gel fabric made by electrospinning method was carried out. Silica gels with four different calcium contents were prepared by condensation following hydrolysis of tetraethyl orthosilicate under acidic conditions. The molar ratios of Ca to Si prepared ranged from 0 to 0.15. SiO<SUB>2</SUB>–CaO gel fabrics were heat-treated at 300°C for 3 h after spinning under an electric field of 2 kV/cm. As the Ca to Si ratio increased, the diameter of electrospun SiO<SUB>2</SUB>–CaO gel fibers increased because the viscosity of the SiO<SUB>2</SUB>–CaO gel solution increased. The apatite-forming ability of heat-treated, non-woven SiO<SUB>2</SUB>–CaO gel fabric was evaluated in simulated body fluid and tended to increase with an increasing Ca to Si molar ratio. However, proliferation and differentiation tended to decrease with an increasing Ca to Si molar ratio. The sample which had the Ca to Si ratio as 0.10 showed good osteoconductivity in vivo in the calvarial defect New Zealand white rabbit model compared to that had the Ca to Si ratio as 0 and empty defect. These results strongly suggest that non-woven SiO<SUB>2</SUB>–CaO gel fabric made by the electrospinning method has potential for application as a bone grafting material. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009</P>

Osteoconductive and degradable electrospun nonwoven poly(ϵ-caprolactone)/CaO-SiO₂ gel composite fabric

Seol, Yang-Jo,Kim, Kyoung-Hwa,Kim, In Ae,Rhee, Sang-Hoon Wiley Subscription Services, Inc., A Wiley Company 2010 Journal of biomedical materials research. Part A Vol.a94 No.2

<P>A nonwoven ceramic/polymer composite fabric composed of randomly mixed bioactive and fast degradable CaO-SiO<SUB>2</SUB> gel fibers and biodegradable poly(ϵ-caprolactone) (PCL) fibers is prepared with a simultaneous electrospinning method for potential use as bone grafting materials. A 17% PCL solution is prepared using 1,1,3,3-hexafluoro-2-propanol as the solvent, whereas the CaO-SiO<SUB>2</SUB> gel solution is prepared via a condensation reaction following the hydrolysis of tetraethyl orthosilicate. PCL and CaO-SiO<SUB>2</SUB> gel solutions are spun simultaneously with two separate nozzles. As controls, pure PCL and CaO-SiO<SUB>2</SUB> gel nonwoven fabrics are also made by the same methods. The three nonwoven fabrics were exposed to simulated body fluid for 1 week and resulted in the deposition of a layer of apatite crystals on the surfaces of both the CaO-SiO<SUB>2</SUB> gel and PCL/CaO-SiO<SUB>2</SUB> gel composite fabrics, but not on the PCL fabric. A tensile strength test showed that the fracture behavior of the CaO-SiO<SUB>2</SUB> gel fabric was brittle, that of the PCL fabric was ductile-tough, and that of the PCL/CaO-SiO<SUB>2</SUB> gel composite fabric was intermediate between that of the CaO-SiO<SUB>2</SUB> gel and PCL fabrics. Our in vivo tests showed that the CaO-SiO<SUB>2</SUB> gel and PCL/CaO-SiO<SUB>2</SUB> gel composite fabrics had good osteoconductivity and fast degradation rates in calvarial defects of New Zealand white rabbits within 4 weeks, in contrast to the pure PCL fabric. Together, these results suggest that the composite fabric composed of PCL and CaO-SiO<SUB>2</SUB> gel fibers must have a great potential for use in applications such as bone grafting because of its good osteoconductivity and adequate mechanical properties. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010</P>

Chitosan sponges as tissue engineering scaffolds for bone formation

Seol, Yang-Jo,Lee, Jue-Yeon,Park, Yoon-Jeong,Lee, Yong-Moo,Young-Ku,Rhyu, In-Chul,Lee, Seung-jin,Han, Soo-Boo,Chung, Chong-Pyoung 이화여자대학교 약학연구소 2004 藥學硏究論文集 Vol.- No.14

Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 ㎛ pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4×10^(6)(day 1), 11×10^(6)(day 28) and 12×10^(6)(day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) ㎍ per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitotsan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.

Rare minisatellite alleles of MUC2-MS8 influence susceptibility to rectal carcinoma

Seol So-Young,Yang Gi-Eun,Cho Yoon,Kim Min Chan,Choi Hong-Jo,Choi Yung Hyun,임선희 한국유전학회 2021 Genes & Genomics Vol.43 No.12

Background Previously, we identifed eight novel minisatellites in the MUC2, of which allelic variants in MUC2-MS6 were examined to infuence susceptibility to gastric cancer. However, studies on the susceptibility to gastrointestinal cancer of other minisatellites in the MUC2 region still remain unprogressive. Objective In this study, we investigated whether polymorphic variations in the MUC2-MS8 region are related to susceptibility to gastrointestinal cancer. Methods We assessed the association between MUC2-MS8 and gastrointestinal cancers by a case–control study with 1229 controls, 486 gastric cancer cases, 220 colon cancer cases and 278 rectal cancer cases. To investigate whether intronic minisatellites afect gene expression, various minisatellites were inserted into the luciferase-reporter vector and their expression levels were examined. We also examined the length of MUC2-MS8 alleles in blood and cancer tissue matching samples of 107 gastric cancer patients, 125 colon cancer patients, and 85 rectal cancer patients, and investigated whether the repeat sequence afects genome instability. Results A statistically signifcant association was identifed between rare MUC2-MS8 alleles and the occurrence of rectal cancer: odds ratio (OR), 6.66; 95% confdence interval (CI), 1.11–39.96; and P=0.0165. In the younger group (age,<55), rare alleles were signifcant associated with an increased risk of rectal cancer (odds ratio, 24.93 and P=0.0001). Suppression of expression was found in the reporter vector inserted with minisatellites, and loss of heterozygosity (LOH) of the MUC2- MS8 region was confrmed in cancer tissues of gastrointestinal cancer patients (0.8–5.9%). Conclusion Our results suggest that the rare alleles of MUC2-MS8 could be used to identify the risk of rectal cancer and that this repeat region is related to genomic instability.

Effect of cell-penetrating peptide-conjugated estrogen-related receptor ${\beta}$ on the development of mouse embryos cultured in vitro

Yang, Ning Jie,Seol, Dong-Won,Jo, Junghyun,Jang, Hyun Mee,Yoon, Sook-Young,Lee, Dong Ryul The Korean Society for Reproductive Medicine 2014 Clinical and Experimental Reproductive Medicine Vol.41 No.1

Objective: Estrogen related receptor ${\beta}$ (Esrrb) is a member of the orphan nuclear receptors and may regulate the expression of pluripotencyrelated genes, such as Oct4 and Nanog. Therefore, in the present study, we have developed a method for delivering exogenous ESRRB recombinant protein into embryos by using cell-penetrating peptide (CPP) conjugation and have analyzed their effect on embryonic development. Methods: Mouse oocytes and embryos were obtained from superovulated mice. The expression of Oct4 mRNA and the cell number of inner cell mass (ICM) in the in vitro-derived and in vivo-derived blastocysts were first analyzed by real time-reverse transcription-polymerase chain reaction and differential staining. Then 8-cell embryos were cultured in KSOM media with or without $2{\mu}g/mL$ CPP-ESRRB protein for 24 to 48 hours, followed by checking their integration into embryos during in vitro culture by Western blot and immunocytochemistry. Results: Expression of Oct4 and the cell number of ICM were lower in the in vitro-derived blastocysts than in the in vivo-derived ones (p<0.05). In the blastocysts derived from the CPP-ESRRB-treated group, expression of Oct4 was greater than in the non-treated groups (p<0.05). Although no difference in embryonic development was observed between the treated and non-treated groups, the cell number of ICM was greater in the CPP-ESRRB-treated group. Conclusion: Treatment of CPP-ESRRB during cultivation could increase embryos' expression of Oct4 and the formation rate of the ICM in the blastocyst. Additionally, an exogenous delivery system of CPP-conjugated protein would be a useful tool for improving embryo culture systems.

Initial adhesion of bone marrow stromal cells to various bone graft substitutes

Jo, Young-Jae,Kim, Kyoung-Hwa,Koo, Ki-Tae,Kim, Tae-Il,Seol, Yang-Jo,Lee, Yong-Moo,Ku, Young,Chung, Chong-Pyoung,Rhyu, In-Chul Korean Academy of Periodontology 2011 Journal of Periodontal & Implant Science Vol.41 No.2

Purpose: The aim of this study is to determine whether certain biomaterials have the potential to support cell attachment. After seeding bone marrow stromal cells onto the biomaterials, we investigated their responses to each material in vitro. Methods: Rat bone marrow derived stromal cells were used. The biomaterials were deproteinized bovine bone mineral (DBBM), DBBM coated with fibronectin (FN), synthetic hydroxyapatite (HA), HA coated with FN, HA coated with $\beta$-tricalcium phosphate (TCP), and pure $\beta$-TCP. With confocal laser scanning microscopy, actin filaments and vinculin were observed after 6, 12, and 24 hours of cell seeding. The morphological features of cells on each biomaterial were observed using scanning electron microscopy at day 1 and 7. Results: The cells on HA/FN and HA spread widely and showed better defined actin cytoskeletons than those on the other biomaterials. At the initial phase, FN seemed to have a favorable effect on cell adhesion. In DBBM, very few cells adhered to the surface. Conclusions: Within the limitations of this study, we can conclude that in contrast with DBBM not supporting cell attachment, HA provided a more favorable environment with respect to cell attachment.

Supplementation With Cell-Penetrating Peptide-Conjugated Estrogen-Related Receptor β Improves the Formation of the Inner Cell Mass and the Development of Vitrified/Warmed Mouse Embryos

Yang, Ning Jie,Seol, Dong-Won,Jo, Junghyun,Jang, Hyun Mee,Yoon, Sook-Young,Lee, Woo Sik,Lee, Dong Ryul Springer-Verlag 2016 Reproductive sciences Vol.23 No.11

경제활동 인구 대상 한국형 삶의 균형 척도 타당화 연구

양희조(YANG, HEE JO),설경옥(SEOL, KYOUNG OK) 강원대학교 사회과학연구원 2020 사회과학연구 Vol.59 No.2

본 연구는 한국인의 삶의 균형을 측정하는 도구를 개발하기 위해 Matuska(2012)의 Life Balance Inventory를 번안 및 수정하여 20세-49세 경제활동 인구를 대상으로 한국형 삶의 균형 척도를 타당화하였다. 연구 1에서는 대학생과 직장인 305명(표본 1)을 대상으로 번안한 원척도 문항에 대한 예비검사를 실시하였다. 탐색적 및 확인적 요인분석 결과 원척도가 제시한 건강, 관계, 정체성, 도전의 4요인에서 보다 세분화된 9개의 요인으로 나타났다. 연구 2는 표본 1과 유사한 성격을 지닌 대학생과 직장인 310명(표본 2)를 대상으로 본 검사를 실시하였다. 확인적 요인분석 결과는 예비검사의 결과와 동일하게 9개의 요인구조가 적절한 것으로 나타났다. 구체적으로 원척도의 건강영역은 기본적 삶유지 요인, 관계영역은 안정적 관계와 새로운 관계 요인, 정체성영역은 개인적 성장과 사회적 성장 요인, 도전영역은 역량개발, 탐험, 취미 그리고 자기표현의 요인으로 세분화되어 나타났다. 준거타당도는 주관적 안녕감, 자율성 기본심리욕구, 삶의 의미발견, 삶의 만족도, 스트레스로 검증하였다. 본 연구는 삶의 균형을 연구할 수 있는 측정 도구를 한국형으로 타당화하여 우리사회에 중요한 화두인 균형있는 삶의 국내 연구 확산을 가능하게 한 의의가 있다. This study aimed to validate the Korean Life Balance scale using Matuska’s (2010) Life Balance Inventory among 20-50 years old who actively engage in economic activities. In Study 1, we performed exploratory and confirmatory factor analyses with 305 college students and employees (Sample 1) after translating and revising the original scale items in Korea. As result, a 9 factor model was supported. The 9 factor model was corresponded with the original 4 factor model. In Study 2, we cross-validated the result of study 1 with 310 college students and employees (Sample 2). A confirmatory factor analysis confirmed the 9–factor model from study 1. The criterion-related validity was confirmed: the Korean Life Balance Scale was positively correlated with subjective well-being, autonomy basic psychological need, found of meaning in life, and life satisfaction. These findings suggest that the Korean Life Balance scale is a reliable and valid measure for assessing life balance in Korea.

Reports : Identification of small molecules that inhibit the histone chaperone Asf1 and its chromatin function

( Ja Hwan Seol ),( Tae Yang Song ),( Se Eun Oh ),( Chanhee Jo ),( Ahreum Choi ),( Byungho Kim ),( Jinyoung Park ),( Suji Hong ),( Ilrang Song ),( Kwan Young Jung ),( Jae Hyun Yang ),( Hwangseo Park ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.12

The eukaryotic genome is packed into chromatin, which is important for the genomic integrity and gene regulation. Chromatin structures are maintained through assembly and disassembly of nucleosomes catalyzed by histone chaperones. Asf1 (anti-silencing function 1) is a highly conserved histone chaperone that mediates histone transfer on/off DNA and promotes histone H3 lysine 56 acetylation at globular core domain of histone H3. To elucidate the role of Asf1 in the modulation of chromatin structure, we screened and identified small molecules that inhibit Asf1 and H3K56 acetylation without affecting other histone modifications. These pyrimidine- 2,4,6-trione derivative molecules inhibited the nucleosome assembly mediated by Asf1 in vitro, and reduced the H3K56 acetylation in HeLa cells. Furthermore, production of HSV viral particles was reduced by these compounds. As Asf1 is implicated in genome integrity, cell proliferation, and cancer, current Asf1 inhibitor molecules may offer an opportunity for the therapeutic development for treatment of diseases. [BMB Reports 2015; 48(12): 685-690]