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Subcellular distribution of chromogranins A and B in bovine adrenal chromaffin cells
Huh, Yang Hoon,Bahk, Sook Jin,Ghee, Jung Yeon,Yoo, Seung Hyun Elsevier 2005 FEBS letters Vol.579 No.23
<P><B>Abstract</B></P><P>The major secretory granule proteins chromogranins A (CGA) and B (CGB) have recently been shown to play critical roles in inositol 1,4,5-trisphosphate-dependent intracellular Ca<SUP>2+</SUP> mobilizations. We determined here the subcellular distribution of CGA and CGB based on 3D-images of chromaffin cells, and found that ∼95% of cellular CGA was present in secretory granules while ∼5% was in the endoplasmic reticulum (ER), whereas ∼57% of cellular CGB was in secretory granules while ∼24% and ∼19% were in the ER and nucleus, respectively. These results suggest that chromogranins are at the center of intracellular Ca<SUP>2+</SUP> homeostasis in secretory cells.</P>
Huh, Yang Hoon,Lim, Do-Sun,Chung, Kyung Hwun,Kwon, Sun Bang,Jeong, Moon Jin,Jeong, Seong Eun,Yoo, Chong Myong 한국곤충학회 2001 Entomological Research Vol.31 No.2
담배나방의 정소와 상부수정관의 발생 및 정소에서의 정자 이동 양상을 구조 분석을 통해 연구하였다. 각 콩 모양의 유충 정소는 interfollicular septa에 의해 구획되는 4개의 소엽으로 이루어져 있으며, 두 개의 유충 정소는 전용기에 총 8개의 소엽으로 이루어지는 단일 정소로 융합된다. 정소의 융합 직후 비틀림 작용이 일어나 구형의 용-성충기 정소가 형성된다. 정소의 발생동안 정소 내 각 소엽들은 정문으로 집중되는 수정세관들을 통해 상부수정관과 연결된다. 성충기 정소의 상부수정관 벽은 잘 발달된 상피세포들과 이를 감싸는 얇은 단일 근육층으로 이루어져있다. 용 기간 동안 나비목 곤충에서 전형적으로 나타나는 eupyrene정자와 apyrene 정자들이 각 포낭 내에서 분화된다. 사정이 개시됨에 따라 apyrene 포낭들이 eupyrene 포낭들 보다 먼저 정소의 기저막을 통과하며, 이때 apyrene정자들은 포낭으로부터 분리된다. 방출된 eupyrene 정자들은 두꺼운 신월형 외막을 새로이 획득하고, reticular appendage를 계속 지니나 lacinate appendage들은 모두 소실된다. 이상의 형태학적 결과들로 미루어, apyrene정자들이 eupyrene 정자가 정소로부터 수정관으로 이동하는 것을 촉진하는 것으로 판단된다. Developmental profiles of testis and upper vas deferens and aspects of sperm migration from testis in the oriental tobacco budworm, Helicoverpa assulta were investigated through fine structural analysis. The larval testes are bean-shaped, and each one consists of four follicles comparted by interfollicular septa. The two larval testes are fused into a single one consisting of eight follicles at prepupal stage. A torsion force generates in the fused testis immediately lifter the fusion, resulting in a spherical pupal-adult testis. During the testicular development, each intratesticular follicle is connected with upper vas deferens via vas efferens converged at the hilum. In adult, the membranes of upper vas deferens consist of well developed epithelial cells and a thin outer muscle layer. Typical lepidopteran eupyrene and apyrene sperms are differentiated in each of their cysts during pupal development. At ejaculation stage, the apyrene cysts penetrate the basement membrane earlier than eupyrene cysts, and the apyrene sperms are free of their cysts in vas deferens during the penetration. The released eupyrene sperms acquire a new thick coat in crescent shape, and still retain the proper reticular appendages, but lose all the lacinate appendages in vas deferens. From our morphological results, it is considered that the apyrene sperm may facilitate the migration of the eupyrene sperm from the testis to the vas deferens.
Huh, Yang Hoon,Cohen, Justin,Sherley, James L. National Academy of Sciences 2013 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.110 No.42
<P>Immortal strands are the targeted chromosomal DNA strands of nonrandom sister chromatid segregation, a mitotic chromosome segregation pattern unique to asymmetrically self-renewing distributed stem cells (DSCs). By nonrandom segregation, immortal DNA strands become the oldest DNA strands in asymmetrically self-renewing DSCs. Nonrandom segregation of immortal DNA strands may limit DSC mutagenesis, preserve DSC fate, and contribute to DSC aging. The mechanisms responsible for specification and maintenance of immortal DNA strands are unknown. To discover clues to these mechanisms, we investigated the 5-methylcytosine and 5-hydroxymethylcytosine (5hmC) content on chromosomes in mouse hair follicle DSCs during nonrandom segregation. Although 5-methylcytosine content did not differ significantly, the relative content of 5hmC was significantly higher in chromosomes containing immortal DNA strands than in opposed mitotic chromosomes containing younger mortal DNA strands. The difference in relative 5hmC content was caused by the loss of 5hmC from mortal chromosomes. These findings implicate higher 5hmC as a specific molecular determinant of immortal DNA strand chromosomes. Because 5hmC is an intermediate during DNA demethylation, we propose a ten-eleven translocase enzyme mechanism for both the specification and maintenance of nonrandomly segregated immortal DNA strands. The proposed mechanism reveals a means by which DSCs “know” the generational age of immortal DNA strands. The mechanism is supported by molecular expression data and accounts for the selection of newly replicated DNA strands when nonrandom segregation is initiated. These mechanistic insights also provide a possible basis for another characteristic property of immortal DNA strands, their guanine ribonucleotide dependency.</P>
게임과몰입 개선을 위한 신체활동 기반 VR 게이미피케이션 개발
허정훈(Jung-Hoon, Huh),이제욱(Jea-Woog, Lee),방큰별(Keun-Byul, Bang),박성제(Sung-Je, Park),이현우(Hyun-Woo, Lee),한정규(Joung-Kyue, Han),양준석(Joon-Seok, Yang),고경훈(Kyung-Hoon, Ko),유지미(Ji-Mi, Yu),김승철(Seung-Chul, Kim),박상국( 한국HCI학회 2020 한국HCI학회 학술대회 Vol.2020 No.2
청소년 게임과몰입에 대한 문제는 그 원인 중 하나로써 게임자체 보다는 게임과몰입 청소년의 환경, 개인적 특성 등에 중점을 두고 개선하고자 하는 다양한 방법을 시도해오고 있다. 이 가운데 운동을 통한 게임과몰입의 원인인 청소년의 심리·정신의학적 기전에 효과적임을 검증하였고, 각 기전에 대한 세분화된 운동치유 방법을 본 연구에서 정립하고자 한다. 즉, 정립된 운동 프로그램을 게임콘텐츠로 적용하여 게임과몰입 청소년들의 인지기능 개선, 기분상승, 사회성 향상, 일반 게임시간 대체 등의 효과를 불러일으키는 “게이미피게이션(Gamification)” 개념을 실천하는 것이 게임과몰입 및 게임에 대한 부정적 사회인식을 개선하는데 세부적인 목적이 있다.
SECTIONAL SURVATURES OF THE SIEGEL-JACOBI SPACE
Yang, Jae-Hyun,Yong, Young-Hoon,Huh, Su-Na,Shin, Jung-Hee,Min, Gil-Hong Korean Mathematical Society 2013 대한수학회보 Vol.50 No.3
In this paper, we compute the sectional curvatures and the scalar curvature of the Siegel-Jacobi space $\mathb{H}_1{\times}\mathb{C}$ of degree 1 and index 1 explicitly.
梁泰天,金炳元,許勇勳 慶星大學校 統一問題硏究所 1998 統一論叢 Vol.4 No.-
It is regrettable that by this time the studies about North Korea have been mostly centralized on the politics, ideology, power structure, military affairs and foreign policies, and there are few studies about the behavior of the bureaucrats in North Korea. Under this circumstance the aim of this paper is to study the behavior of the bureaucrats in North Korea. This paper tries to examine empirically the characteristics and aspects of change, of the behavior of the bureaucrats in North Korea on the basis of the content analysis of the Rodong Shinmoon, the official party paper in North Korea and Kim Ⅱ Sung's writings published in North Korea. For this purpose first we classify the patterns of the bureaucrats in North Korea as party bureaucrat, administrative bureaucrat, economic bureaucrat and bureaucrat in general. Second we select the behavior of the bureaucrats in North Korea as follows: authoritarianism, formalism, sectionalism, negativism, overconformity, expert and red. Finally we analyze with the Relative Deviation Index how each of this behavior has been changed during the given periods( 1963?984) relating to each of the patterns of the bureaucrats in North Korea.
Yang, Hyo-Seung,Park, Hoon,Kim, Hyoung-chul,Ahn, Jae-Pyoung,Huh, Moo-Young,Park, Jong-Ku The Korean Powder Metallurgy Institute 2004 한국분말재료학회지 (KPMI) Vol.11 No.3
We report a carbothermal reduction process for massive synthesis of monolithic W$_{18}$O$_{49}$ phase from tungsten oxide in the presence of carbon source. Carbon black powder was used as a carbon source and added to WO$_3$ by 40 weight percent. Bundles of W$_{18}$O$_{49}$ rods were formed over the temperature range of 80$0^{\circ}C$$^{\circ}C$ to 90$0^{\circ}C$. Pure W$_{18}$O$_{49}$ bundles could be separated from the mixture of W$_{18}$O$_{49}$ and residual carbon black powder. Field emission character of W$_{18}$O$_{49}$ phase was determined using the extracted W$_{18}$O$_{49}$ rods. Flat lamp fabricated from the W$_{18}$O$_{49}$ rods showed the turn-on field of 9.3 V/${\mu}m$.