CRM1 inhibitor S109 suppresses cell proliferation and induces cell cycle arrest in renal cancer cells

Liu, Xuejiao,Chong, Yulong,Liu, Huize,Han, Yan,Niu, Mingshan The Korean Society of Pharmacology 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.2

Abnormal localization of tumor suppressor proteins is a common feature of renal cancer. Nuclear export of these tumor suppressor proteins is mediated by chromosome region maintenance-1 (CRM1). Here, we investigated the antitumor effects of a novel reversible inhibitor of CRM1 on renal cancer cells. We found that S109 inhibits the CRM1-mediated nuclear export of RanBP1 and reduces protein levels of CRM1. Furthermore, the inhibitory effects of S109 on CRM1 is reversible. Our data demonstrated that S109 significantly inhibits proliferation and colony formation of renal cancer cells. Cell cycle assay showed that S109 induced G1-phase arrest, followed by the reduction of Cyclin D1 and increased expression of p53 and p21. We also found that S109 induces nuclear accumulation of tumor suppressor proteins, Foxo1 and p27. Most importantly, mutation of CRM1 at Cys528 position abolished the effects of S109. Taken together, our results indicate that CRM1 is a therapeutic target in renal cancer and the novel reversible CRM1 inhibitor S109 can act as a promising candidate for renal cancer therapy.

CRM1 inhibitor S109 suppresses cell proliferation and induces cell cycle arrest in renal cancer cells

Xuejiao Liu,Yulong Chong,Huize Liu,Yan Han,Mingshan Niu 대한생리학회-대한약리학회 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.2

Abnormal localization of tumor suppressor proteins is a common feature of renal cancer. Nuclear export of these tumor suppressor proteins is mediated by chromosome region maintenance-1 (CRM1). Here, we investigated the antitumor effects of a novel reversible inhibitor of CRM1 on renal cancer cells. We found that S109 inhibits the CRM1-mediated nuclear export of RanBP1 and reduces protein levels of CRM1. Furthermore, the inhibitory effect of S109 on CRM1 is reversible. Our data demonstrated that S109 significantly inhibits proliferation and colony formation of renal cancer cells. Cell cycle assay showed that S109 induced G1-phase arrest, followed by the reduction of Cyclin D1 and increased expression of p53 and p21. We also found that S109 induces nuclear accumulation of tumor suppressor proteins, Foxo1 and p27. Most importantly, mutation of CRM1 at Cys528 position abolished the effects of S109. Taken together, our results indicate that CRM1 is a therapeutic target in renal cancer and the novel reversible CRM1 inhibitor S109 can act as a promising candidate for renal cancer therapy.

Design and Prediction of Three Dimensional Flows in a Low Speed Highly Loaded Axial Flow Fan

Liu, Xuejiao,Chen, Liu,Dai, Ren,Yang, Ailing Korean Society for Fluid machinery 2013 International journal of fluid machinery and syste Vol.6 No.2

This paper describes the design to increase the blade loading factor of a low speed axial flow fan from normal 0.42 to highly loaded 0.55. A three-dimensional viscous solver is used to model the flows in the highly-loaded and normal loaded stages over its operation range. At the design point operation the static pressure rise can be increased by 20 percent with a deficit of efficiency by 0.3 percent. In the highly loaded fan stage, the rotor hub flow stalls, and separation vortex extends over the rotor hub region. The backflow, which occurs along the stator hub-suction surface, changes the exit flow from the prescribed axial direction. Results in this paper confirm that the limitation of the two dimensional diffusion does not affect primarily on the fan's performance. Highly loaded fan may have actually better performance than its two dimensional design. Three dimensional designing approaches may lead to better highly loaded fan with controlled rotor hub stall.

Electrically Conductive Graphene-Based Biodegradable Polymer Composite Films with High Thermal Stability and Flexibility

Xuejiao Liu,Qinfeng Zou,Tianhao Wang,Liping Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.3

Cellulose nanofibril (CNF) and graphene (GR) powder were added into polylactic acid (PLA)/ polypyrrole (PPy) composite films via a low-cost, eco-friendly, low-temperature, and in-situ polymerization synthesis, which obtain novel flexible and conductive polylacticacid-cellulose nanofibril-graphene/polypyrrole (PLA–CNF–GR/PPy) composite films. The CNF was embedded in the PLA matrix to enhance the mechanical properties. Remarkably, when a few GR (1%) powder was added, the tensile strength of composite films increased by 5.6%, respectively, compared with pure PLA–CNF, and increased by 17.6% compared with the PLA. The GR and CNF had a positive influence on mechanical properties of composite films. In addition, the PLA–CNF–GR/PPy composite films exhibited many unique properties when GR powder was introduced, including high thermal stability, and especially electrical conductivity. The electrical conductivity of the PLA–CNF–GR/PPy composite films increased from 0.12 to 1.06 S/cm as the content of GR powder increased from 0 to 10%. The PLA–CNF–GR-10/PPy also demonstrated excellent flexible stability, only 7.5% deviation after over 100 bending cycles. Furthermore, we designed and found that the exploration of a flexible solid-state supercapacitor assembled with PLA–CNF–GR-10/PPy composite electrodes had a capacitance of 30 F/g at a current density of 0.5 A/g. Although it was not quite as prominent as the capacitance, it provided an innovative means for preparing the conductive composite films. Based on these advantages the PLA–CNF–GR/PPy could be considered as sensors, flexible electrodes, and flexible displays. It also opens a new field of potential applications of biodegradable materials.

Design and Prediction of Three Dimensional Flows in a Low Speed Highly Loaded Axial Flow Fan

Xuejiao Liu,Liu Chen,Ren Dai,Ailing Yang 한국유체기계학회 2013 International journal of fluid machinery and syste Vol.6 No.2

This paper describes the design to increase the blade loading factor of a low speed axial flow fan from normal 0.42 to highly loaded 0.55. A three-dimensional viscous solver is used to model the flows in the highly-loaded and normal loaded stages over its operation range. At the design point operation the static pressure rise can be increased by 20 percent with a deficit of efficiency by 0.3 percent. In the highly loaded fan stage, the rotor hub flow stalls, and separation vortex extends over the rotor hub region. The backflow, which occurs along the stator hub-suction surface, changes the exit flow from the prescribed axial direction. Results in this paper confirm that the limitation of the two dimensional diffusion does not affect primarily on the fan's performance. Highly loaded fan may have actually better performance than its two dimensional design. Three dimensional designing approaches may lead to better highly loaded fan with controlled rotor hub stall.

Computer-aided diagnosis system based on multi-scale feature fusion for screening large-scale gastrointestinal diseases

Pang Xuejiao,Zhao Zijian,Wu Yanbing,Chen Yong,Liu Jin 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.1

For endoscopists, large-scale screening of gastrointestinal (GI) diseases is arduous and time-consuming. While their workload and human factor-induced errors can be reduced by computer-aided diagnosis (CAD) systems, the existing ones mainly focus on a limited number of lesions or specific organs, making them unsuitable for diagnosing various GI diseases in large-scale disease screening. This paper proposes a transformer and convolutional neural network-based CAD system (called TransMSF) to assist endoscopists in diagnosing multiple GI diseases. This system constructs two feature extraction paths with different coding methods to obtain the lesions’ global and local information. In addition, downsampling is implemented in transformer to get global information of different scales, further enriching the feature representation and reducing the amount of computation and memory occupation. Moreover, a channel and spatial attention module with fewer parameters was successfully designed to pay more attention to the target and reduce the loss of important information during spatial dimension transformation. Finally, the extracted feature information is fused through the feature fusion module and then input into the linear classifier for disease diagnosis. The proposed system outperformed that of other state-of-the-art models on two datasets, reaching a 98.41% precision, a 98.15% recall, a 98.13% accuracy, and a 98.28% F1 score on the in-house GI dataset versus a 95.88% precision, a 95.88% recall, a 98.97% accuracy, and a 95.88% F1 score on the public Kvasir dataset. Moreover, TransMSF’s performance was superior to that of seasoned endoscopists. The above results prove that the proposed system is instrumental in diagnosing GI diseases in large-scale disease screening. It can also be used as a training tool for junior endoscopists to improve their professional skills by rendering helpful suggestions.

Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)

Tiantian Zhang,Xuejiao Cui,Jincheng Zhang,Hui Wang,Meng Wu,Hua Zeng,Yuanyuan Cao,Jingze Liu,Yonghong Hu 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.6

In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.

Pectin oligosaccharides improved lipid metabolism in white adipose tissue of high-fat diet fed mice

Zixin Fan,Xuejiao Chen,Tianzhi Liu,Qianhui Yu,Ziqi Song,Fei Wang,Tuoping Li 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.9

Impacts of pectin oligosaccharide (POS) got from hawthorn fruitage on adiponectin signaling pathway and white adipose metabolism in mice fed with high-fat control. The results showed that POS significantly inhibited the levels of inflammatory cytokines TNF-α and IL-6, and down-regulated expression of CD68. POS dramatically reduced gene expression contents of fatty acid composite concerning enzymes ACC and FAS, as well as TG synthesis-related enzymes SCD-1 and DGAT1 as compared to a high-fat group (HFC). POS dramatically increased expression levels of oxidation-related enzymes of fatty acid ACO, CPT-1, and TG deposition-related enzymes ATGL and HSL as contrast to the high-fat control group. In addition, POS activated adiponectin-mediated AdipoR1/AMPK/PPARα signaling path by upregulating expression levels of AdipoR1, AMPK and PPARα. The results demonstrated that POS can improve lipid metabolism of adipose tissue, and contribute to the creation of functional foods to prevent and treat lipid metabolism disorders.

Low-temperature solution selective growth of zinc oxide nanorods on different substrates

Jian Zhang,Lijuan Wan,Xuejiao Chen,Chunran Liu,Huina Hou 한양대학교 세라믹연구소 2010 Journal of Ceramic Processing Research Vol.11 No.3

In this paper, regular ZnO nanorods with excellent reproducibility were grown by a simple solution method at low temperature. The growth parameters, which can affect the ZnO nanostructures were studied, including the substrate types, the growth temperature, the growth time and the concentration of the solution. Among these conditions, the influence of different substrates on the resulting nanostructures was investicated carefully. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were used to characterize the morphology and microstructure of the as-grown ZnO nanorods. It is concluded that the substrates can affect the ZnO growth remarkably and the selective growth of ZnO is possible through the choice of the substrates.

An Improved Privacy Preserving Construction for Data Integrity Verification in Cloud Storage

( Yingjie Xia ),( Fubiao Xia ),( Xuejiao Liu ),( Xin Sun ),( Yuncai Liu ),( Yi Ge ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.10

The increasing demand in promoting cloud computing in either business or other areas requires more security of a cloud storage system. Traditional cloud storage systems fail to protect data integrity information (DII), when the interactive messages between the client and the data storage server are sniffed. To protect DII and support public verifiability, we propose a data integrity verification scheme by deploying a designated confirmer signature DCS as a building block. The DCS scheme strikes the balance between public verifiable signatures and zero-knowledge proofs which can address disputes between the cloud storage server and any user, whoever acting as a malicious player during the two-round verification. In addition, our verification scheme remains blockless and stateless, which is important in conducting a secure and efficient cryptosystem. We perform security analysis and performance evaluation on our scheme, and compared with the existing schemes, the results show that our scheme is more secure and efficient.