http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Sequence and functional analysis of an upstream regulatory region of human HOXA7 gene
Min, Wongi,Cho, Myungsun,Jang, Seung Ik,Chang, Hwa-Hyoung,Lee, Chul-Sang,Jun, Moo-hyung,Kim, Myoung Hee 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-
The Hox genes have been known to be involved in pattern formation during vertebrate development through differential expression along the anteroposterior body axis. Human homologue of position-specific regulatory region of murine Hoxa-7 was cloned from human genomic library. The restriction map of the 18-kb insert was determined. of which a 3.9-kb region was sequenced. Homology plot between the murine and the corresponding human sequence showed high sequence conservation over 70% in several regions. The homologous region has been reduced to about 1.1 kb (HCR: human control region), which contained several putative factor binding sites. The function of HCR was analyzed in transgenic mice and turned out to be a position-specific regulatory clement of human, setting the precise anterior boundary of expression in transgenic embryos: at day 12.5 postcoitum a distinct anterior limit of expression was noted at the level of C5 in neural tube and spinal ganglia in transgenic cmbryos. These results indicatc that the regulatory sequences as well as the molecular mechanism for Hox gene expression are highly conserved among vcrtebrates.
Interleukin-17A production in Riemerella anatipestifer infection is independent to IL-23
Flores Rochelle(Flores Rochelle ),Fernandez Cherry(Fernandez Cherry ),Afrin Fahmida(Afrin Fahmida ),Cammayo Paula Leona(Cammayo Paula Leona ),Roy Anindita(Roy Anindita ),Min Wongi(Min Wongi ) 한국예방수의학회 2019 한국예방수의학회 학술대회자료집 Vol.2018 No.-
Kim, Jong-hyeon,Min, Kyoung-Jin,Seol, Wongi,Jou, Ilo,Joe, Eun-hye Blackwell Publishing Ltd 2010 Journal of Neurochemistry Vol.115 No.5
<P><I>J. Neurochem.</I> (2010) <B>115</B>, 1161–1171.</P><P>Abstract</P><P>Microglia are known to be a primary inflammatory cell type in the brain. However, microglial inflammatory responses are attenuated in the injured brain compared to those in cultured pure microglia. In the present study, we found that astrocytes challenged by oxygen–glucose deprivation (OGD) or H<SUB>2</SUB>O<SUB>2</SUB> released soluble factor(s) and attenuated microglial inflammatory responses. Conditioned medium prepared from astrocytes treated with OGD (OGD-ACM) or H<SUB>2</SUB>O<SUB>2</SUB> (H<SUB>2</SUB>O<SUB>2</SUB>-ACM) significantly reduced the levels of interferon-&ggr; (IFN-&ggr;)-induced microglial inflammatory mediators, including inducible nitric oxide synthase, at both the mRNA and protein levels. The anti-inflammatory effect of astrocytes appeared very rapidly (within 5 min), but was not closely correlated with the extent of astrocyte damage. Both OGD-ACM and H<SUB>2</SUB>O<SUB>2</SUB>-ACM inhibited STAT nuclear signaling, as evidenced by a reduction in both STAT-1/3 binding to the IFN-&ggr;-activated site and IFN-&ggr;-activated site promoter activity. However, both phosphorylation and nuclear translocation of STAT-1/3 was unchanged in IFN-&ggr;-treated microglia. The active component(s) in OGD-ACM were smaller than 3 kDa, and displayed anti-inflammatory effects independent of protein synthesis. These results suggest that, in the injured brain, astrocytes may act as a controller to rapidly suppress microglial activation.</P>
Application of biotechnological tools for coccidia vaccine development
Hyun S.Lillehoj,Wongi Min,Rami A.Dalloul 대한수의학회 2004 Journal of Veterinary Science Vol.5 No.4
Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies have relied on prophylactic medication. Due to the continual emergence of drug resistant parasites in the field and increasing incidence of broiler condemnations due to coccidia, novel approaches are urgently needed to reduce economic losses. Understanding the basic biology of hostparasite interactions and protective intestinal immune mechanisms, as well as characterization of host and parasite genes and proteins involved in eliciting protective host responses are crucial for the development of new control strategy. This review will highlight recent developments in coccidiosis research with special emphasis on the utilization of cutting edge techniques in molecular/cell biology, immunology, and functional genomics in coccidia vaccine development. The information will enhance our understanding of host-parasite biology, mucosal immunology, and host and parasite genomics in the development of a practical and effective control strategy against Eimeria and design of nutritional interventions to maximize growth under the stress caused by vaccination or infection. Furthermore, successful identification of quantitative economic traits associated with disease resistance to coccidiosis will provide poultry breeders with a novel selection strategy for development of genetically stable, coccidiosis-resistant chickens, thereby increasing the production efficiency.
Expression of Chicken NK-Lysin and Its Role in Chicken Coccidiosis Induced by Eimeria necatrix
Woo Hyun Kim,Wongi Min,Kwang Il Park,Hyun S. Lillehoj,Cherry P. Fernandez-Colorado,Rochelle A. Flores,Paula Leona T. Cammayo,Binh Thanh Nguyen 대한기생충학열대의학회 2021 The Korean Journal of Parasitology Vol.59 No.5
Anticoccidial effects of Aloe vera-based diets in chickens
Dong jeon Yim(임동진),Sang S. Kang,Wongi Min 한국가금학회 2009 한국가금학회 정기총회 및 학술발표회 Vol.26 No.-
Avain coccidiosis is the most important parasitic disease in the poultry industry and has been mainly controlled by the use of chemotherapeutic agents and live vaccines. Due to emergence of drug-resistant strains and the high cost of vaccines, alternative control strategies are needed. In this study, protective effects of Aloe vera-based diets were assessed in broiler chickens following oral infection with E.maxima. Chickens were divided into five groups (Aloe 0.1 %, Aloe 0.3 %, Aloe 0.5 %, untreated/infected and non-infected control) of 18 chickens each group. Chickens were fed with a regular diet supplemented with ground Aloe vera to the duration of the experiment from 2 days prior to infection with 10,000 sporulated oocysts of E. maxima. Fecal oocyst shedding decreased signicantly (p < 0.001 in all the treatment groups when compared to the untreated group fed with a regular diet. No significant differences were found in body weight between the untreated and Aloe vera-treated groups, although body weight loss caused by E. maxima infection was not improved by Aloe vera-based diets.