DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

The Analysis on the Value of Yongsan National Park and its Economic Effect

Chang, In-Seok,Cho, Young-Tae,Lee, Mi-Hong,Park, Shin-Won Land and Housing Institute 2011 LHI journal of land, housing, and urban affairs Vol.2 No.4

This study estimated economic value and benefits of Yongsan National Park, which will be constructed by financial investment of the government and objectively estimated the real value of it by understanding the value of the park from a user's view of the park. For this, the value of Yongsan National Park is divided into using value such as carbon reduction and the function of relieving urban heat island as an environmental material and non-using value based on willingness to pay of the public according to the construction of the park. As a result of the analysis, it was found out that the using value of Yongsan National Park would reach 130 million won up to maximum level of 450 million won per year, and the non-using value was analyzed to be worthy of 2,344 won per capita every month. Besides, economic ripple effect that can be expected in the process of the park construction project was analyzed to be a national policy that creates effect on production inducement of 2.6 trillion won and value added of 809.6 billion won and new jobs for 25,620 persons. Considering the value of Yongsan National Park and the effect of the project based on the result of this study, it was found that it could sufficiently secure the validity of implementing the project compared to the financial investment by the government. Therefore, it must be emphasized that diffusion strategy is necessary for national understanding and for a nation to make it understood its appropriateness widely in respect to the construction of Yongsan National Park for the successful construction of Yongsan National Park and to raise its using value in the future.

도시공원 바닥포장의 디자인 개선에 관한 연구

김신원(Shin Won Kim),박태석(Tae Seok Park),노희곤(Hee Gon Noh) 한국디자인문화학회 2009 한국디자인문화학회지 Vol.15 No.3

도시가 발전하면서 사람들은 삶에 있어 정신적, 문화적 욕구의 충족에 더 많은 관심을 가지게 된다. 도시환경을 개선하고자 하는 요구는 과거에 비해 더욱 심화되었다. 조경 분야에서 외부 환경의 물리적 요소는 이러한 요구에 부응하는데 있어 중요한 역할을 한다. 일반적으로 외부 공간을 형성하는 주요한 골격은 토지, 식물, 정형적 요소 등이다. 도시생활에서 이용자가 향유하는 공간으로는 주로 자연이 우세한 자연적 공간, 인공적 요소가 가미된 자연적 공간, 인공성이 주가 되는 자연적 공간 등이 있다. 이러한 공간은 사람들이 자연과 접하며 휴식하고 만나고 담소를 나누는 장소가 된다. 외부 공간은 실용적인 기능뿐만이 아니라 시각적인 아름다움도 지녀야 한다. 최근 들어 조경디자인 분야에서 공간을 구성하는데 질 높은 다양한 재료들이 활용되고 있다. 그런데 도시공원을 보면 특히 바닥포장과 관련해서는 개선해야 할 부분이 많이 있다. 시대의 흐름 및 도시민의 요구에 부응하여 빠른 시일 내에 도시공원 바닥포장 디자인의 개선이 이루어져야 하고 재료 선정에 변화가 있어야 한다. 도시공원의 바닥포장은 이용자의 쾌적성, 안전성, 접근성 등에 영향을 준다. 도시공원 바닥포장의 디자인 개선에 관한 본 연구는 쾌적한 도시공원을 위해 필요로 하는 바닥포장의 조성에 대한 연구이다. 연구방법은 선유도, 서울숲, 월드컵공원의 현황을 조사하여 바닥포장과 관련된 문제의 요인과 유형을 분석하고, 문제를 해결하고 공간의 질을 높이기 위해 바닥포장 개선 방안을 연구하여 설계안을 제시하였다. 도시공원 바닥포장의 품질 개선을 도모하며, 향후 도시공원 바닥포장 디자인의 수준을 제고하는데 유용하게 활용될 수 있는 자료를 제공하는 것에 본 연구의 의의와 목적이 있다. 연구를 수행하기 위하여 도시공원 바닥포장에 대해 현황 조사 및 분석이 이루어졌다. 또한 연구 결과를 토대로 하여 바람직한 바닥포장 재료 및 색채를 선정하고 적절한 바닥포장 디자인의 변경안을 제시하였다. 도시공원 바닥포장의 디자인 변경이 요구되는 현황을 조사하고 그 원인을 분석하여 개선안을 제시한 본 연구의 내용은 이용자가 만족할 수 있는 쾌적하고 아름다운 도시공간을 조성하는데 도움이 될 것이다. As cities continue to develop, people desire to fulfil their mental and cultural desires. So the demand for improving the city environment is more palatable than ever. The `outer-space` physical element in landscape architecture has its own role to play in satisfying this demand. The general frame of `outer-space` is mostly based on land, plants, and formative factors, regardless of size, fixedness, or location. The space for leisure use is divided into three parts. The first is `natural space` including mountains, rivers, and seas. The second is `natural space with added artificial elements` such as parks and terraced land on the rivers, which are created to protect the natural space from probable damage and to make full use of the space as rest areas. The third is `purposeful space` such as squares and parks with environmental and human facilities. They are the spaces made for the purposes of resting, recreation, and/or indicating the border of inside or outside of buildings. The ideal function of the elements of outer space is not only providing beauty, but also playing an important role in determining the atmosphere of limited space like parks. With the rapidly changing social norm, the design period tends to get shorter and shorter, and the recent trend is to use materials with high quality and diversity. However, the design of pavement in city parks has not been remodeled. Great improvements to facility maintenance, repair work, and plant preservation in parks have been made, but the issue of remodelling pavement has hardly been considered. Given that the type of pavement can give comfort or stress to the people visiting parks, it plays an important role in park design. This study is to design and create surface pavement for improving the outdoor spatial quality of city parks. The specific goals and objectives of this study are: to develop design criteria for creating user-responsive outdoor space, based upon the research findings drawn from site survey and analysis focusing on the surface pavement in city parks; and to make specific design recommendations, if there is room for improvement, for improving the outdoor spatial quality of city parks. This research study focused on surface pavement in 3 city parks (Seonyu Island, Seoul Forest, World Cup Park) such as overall image, arrangement of pavement, design and pattern, material, and color. With this background, and with survey and analysis, this study will suggest design changes to pavement in order to make the public space of the city park more satisfying and comfortable to its users. Findings obtained from this research study could be used in the designing of future projects in order to get a better understanding of the needs of users. The changes in order to meet users` needs and to improve the spatial quality of the city parks should be implemented.

Development of a Mobile Application, "Wild Flowers of Bukhansan National Park (version 1.0)", for Identification of Plants in Bukhansan National Park

Kim, Sang-Tae,Lee, Seung-Yeon,Kim, Seung-Chul,Byun, Hye-Won,Lee, Sang-Tae,Kim, Mu-Yeal,Hong, Seok-Pyo,Chung, Young-Jae,Park, Ki-Ryong,Lee, Chung-Hee,Lee, Joong-Ku,Heo, Kyeong-In,Lee, Ji-Ye,Lee, Eun-Je National Science Museum of Korea 2011 Journal of Korean nature Vol.4 No.3

We developed the educational purpose mobile application, named "Wild Flowers of Bukhansan National Park (version 1.0)", aiming for easy identification of wild flowers for students and visitors in the park. When visitors find a flower or part of plant in the park, visitors can search for its name utilizing the pictures and characters provided in their own smartphone mobile devices or tablet PCs. The application provides pictures of wild flowers in the park and character-based searching system based on 12 diagnostic features (e.g., growth form, leaf arrangement, flower symmetry, petal color, petal number, sepal number, etc). We adopted the complete floristic survey of Chung and Lee (1962) and added species that we confirmed their distribution in the park during the development of this application. In summary, number of vascular plants in this park was estimated to be 428 taxa including 100 families, 280 genera, 327 species, 1 subspecies, 50 varieties, and 5 formas. We provided a total of 588 pictures representing 358 taxa and each taxon includes multiple pictures in many cases. Included identification quizzes can be an efficient educational tool as well as fun activity for students and visitors who are learning plant species in Korea. Our next step will include GPS function in the application for indicating visitor's location and for providing previously reported sites of the species that we interested in the map of the park. The future application which includes GPS function will be a valuable tool for the monitoring of rare plants, plant researches related to the climate changes, etc. We currently provide Korean iPhone version only, and English version and both of android versions will be serviced soon.

간세포암과 감별이 어려웠던 악성중피종 치험 1예

김선문,허원석,채경훈,강윤세,정재훈,김연수,박기오,문희석,이엄석,김석현,성재규,이병석,이헌영,신경숙,조준식,송인상,강대영 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Malignant peritoneal mesothelioma is a rare neoplasm that arises from the mesothelium of a serosal cavity and is a rapidly fatal disease with a median survival of 4 to 12 months for untreated cases. Recently, we experienced a case with malignant peritoneal mesothelioma who was suspected hepatocelluar carcioma by abdominal CT scan and was confirmed by biopsy including immunohistochemical stain(calretinin) after surgery. We performed tumor excisions and wedge resection of the liver(segment Ⅷ)and inserted Tencoff catheter in abdominal cavity at 25th day of post-operation. We treated with intraperitoneal paclitaxel(25mg/m^(2)/day for 5 days) six courses monthly. She was well tolerable and is still living without any evidence of recurrence for 14th month of post-operation.

건조방법에 따른 하수슬러지 건조속도 비교 및 경제성 평가

박성훈 ( Seong-hun Park ),김한석 ( Han-seok Kim ),이승원 ( Seung-won Lee ) 한국환경기술학회 2021 한국환경기술학회지 Vol.22 No.3

In this study investigated the effect of the chemical injection ratio on the drying rate of sewage sludge. Then the economic efficiency was evaluated by comparing the results of the NIR(near infrared ray) and microwave drying rate test. The near infrared ray device showed drying speed of 31.9 %, 28.1 %, and 27.1 % at polymer dose ratio of 8 %, 10 %, and 12 %, respectively. Compared to 8 % polymer dose ratio, polymer dose ratio of 10 % and 12 % showed differences of 8.0 % and 13.6 % respectively, suggesting that drying efficiency decreased as polymer dose ratio increased. The microwave device showed drying speed of 26.2 %, 24.3 %, 21.3 % at polymer dose ratio of 8 %, 10 %, and 12 %, respectively. Compared to 8 % polymer dose ratio, polymer dose ratio of 10 % and 12 % showed differences of 5.2 % and 13.4 % respectively, suggesting that drying efficiency decreased as polymer dose ratio increased. Drying evaluation estimated the treatment cost of near infrared ray device at optimal dose ratio(6 %) was 35,591 won/ton while that of actual dose ratio(8 %) was 38,928 won/ton. Drying evaluation estimated treatment cost of microwave device at optimal polymer dose ratio(6 %) and at 8 % to be 16,683 won/ton and 18,352 won/ton, respectively.

국소성 분절성 사구체 경화증의 완전 관해 후 발병한 IgA 신병증 1예

임현민,김현정,박민석,주혜원,최원,김상현,박원도 인제대학교 2006 仁濟醫學 Vol.27 No.-

We report a rare case of primary glomerular disease with IgA nephropathy in a 43-year old man. 7-years ago, he was diagnosed with minimal change nephrotic syndrome. After treatment with steroid for 1-year, azotemia and proteinuria were improved. But, nephrotic range proteinuria was relapsed within 1 week after cessation of steroid. Renal biopsy revealed focal segmental glomerulosclerosis, and achived a complete remission with the use of steroids and cyclophosphamide. Then, he was followed up with normal renal function for 4-years. On admission, the patient showed generalized edema and marked proteinuria. In 3rd renal biopsy, mesangial proliferative glomerulonephritis with diffuse global sclerosis was observed. Electron microscopy showed electron-dense deposit on the mesangium with slight mesangial proliferation, and immunofluoroscence microscopy showed granular deposits of IgA and IgM. These features were consistent with IgA nephropathy, different from results of previous biopsies. The pathogenesis of IgA nephropathy occurred with primary FSGS is not clear, as well as treatment. Now, proteinuria is improving, steroid and cyclophosphamide are probably useful for the disease.

국립공원 아고산대 독립훼손지 복원식생 분포특성 및 관리방안

김종엽(Jong-Yup Kim),윤주웅(Ju-Ung Yun),한봉호(Bong-Ho Han),곽정인(Jeong-In Kwak),박석철(Seok-Cheol Park),박수영(Su Young Park),정원옥(Won-Ok Jeong) 국립공원연구원 2022 국립공원연구지 Vol.13 No.1

본 연구는 독립훼손지 중 아고산대에 위치한 지리산국립공원 세석평전과 노고단 복원식생지를 대상으로 현존식생 분석 및 식생분포 특성을 파악하여 중장기적인 모니터링 및 관리방안 수립을 위한 기초자료를 제공하고자 수행하였다. 현존식생 조사범위는 훼손지 일대 유역권분포 현황을 고려하여 설정하였으며, 세석평전 조사면적은 55,037㎡, 노고단 조사면적은 166,599㎡ 이었다. 세석평전은 초본층에 의한 식생피복도는 90% 이상 수준으로서 나지노출이 없었고, 관목층의 피복도가 60% 이상으로 안정화되어 있었다. 주요 출현수종은 구상나무, 사스래나무, 쇠물푸레나무, 철쭉, 털진달래, 붉은병꽃나무 등 식재수종과 자연적으로 이입한 자생수종이 생육하고 있었다. 노고단은 신갈나무, 호랑버들, 철쭉, 털진달래, 미역줄나무 등이 우점하는 관목식생지가 52%로 가장 넓게 분포하고 있었고, 전반적으로 식생활착 상태가 양호하였다. 노고단의 원식생은 신갈나무군락으로 추정되었고, 군부대철거지에는 귀화식물이 분포하고 있기 때문에 적극적인 관리가 필요하였다. 연구대상지는 장기적으로 주변 자연식생과 조화로운 식생경관으로 발달할 것으로 예측되었다. 향후 훼손지 복원모델은 인근 지역의 원식생을 고려하여 설정해야 할 것이지만, 복원 달성 목표는 실현가능한 수준의 목표를 설정해야 할 것이다. This study was conducted to provide basic data for mid-to long-term monitoring and establishment of management plans by analyzing the existing vegetation and identifying the vegetation distribution characteristics for the restored vegetation in Nogodan and Seseokpyeongjeon in Jirisan National Park located in the subalpine zone among the independent damaged areas. Actual vegetation survey range was established in consideration of the distribution of the watershed around the damaged area. The surveyed area of Seseokpyeongjeon was 55,037㎡, and the surveyed area of Nogodan was 166,599㎡. In Seseokpyeongjeon, the vegetation coverage by the herbaceous layer was almost 90% or more, there was no bare exposure, and the coverage of the shrub layer was stabilized at 60% or more. The main emerging tree species were planted species such as Abies koreana, Betula ermanii, Fraxinus sieboldiana, Rhododendron schlippenbachii, Rhododendron mucronulatum var. ciliatum, and Weigela florida as well as natively migrated native species. In Nogodan, the shrub vegetation dominated by Quercus mongolica, Salix caprea, Rhododendron schlippenbachii, Rhododendron mucronulatum var. ciliatum, and Tripterygium regelii was the most widely distributed with 52% of the area, and the overall diet was good. The native vegetation of Nogodan was presumed to be a cypress colony. Naturalized plants were distributed in the military base demolition site, so active management was required. The study site was predicted to develop into a vegetation landscape in harmony with the surrounding natural vegetation in the long term. In the future, the restoration model for damaged areas should be set in consideration of the native vegetation of the nearby area, but the goal to achieve restoration should be set at a feasible level.

사망진단서(사체검안서) 상의 선행사인으로부터 사망통계의 원사인이 선정되는 비율 : 3개 대학병원에서 교부된 사망진단서를 중심으로

박우성,박석건,정철원,김우철,탁우택,김부연,서순원,김광환,서진숙,부유경 한국의료QA학회 2004 한국의료질향상학회지 Vol.11 No.1

Background : To exatnine the problems intolved in writing practice of death certificates, we cotnpated the determination of underlying cause of death for wital statistics using recorded underlying cause of death in issued death statistics. Methods : We collected 688 rnortality certificates issue in year of 2,000 from 3 university hospitals. And we also collected vital statistics from ministry of statistics. The causes of death were coded by experienced medical record wpecialists. And causes of death determined at ministry of statistics for national vita statistics were mapped to causes of death recorded at each death certificates. The rate that underlying causes of death for vital statistics were derived from underlying causes of death recorded at issued death certificaties sere analysed. Results : 64.5% of underlying cause of death for could be derived from underlying cause of death recorded at issued death certificates, 8.6% derived from intermediate cause of death, and 3.9% derived from direct cause of death. In 23% of cases, underlying cause of death could not be derived using issued death certificates. The rate that underlying cause of death for vital statistics could be derived from underlying cause of death recorded at death certificates was different between 3 university hospitals. Ane the rate was also different between death certificates and postmortem certificates. We classified the causes of death using 21 major categories. The rate was different between diseases or conditions tha caused death too. Conclusion : When we examined the correctness of death certificate writing practice using abpve methods, cortectness of writing could not be told as satisfactory. There was difference in correctness of writing between hospotals, between death certificates and postmortem certificates, and between diseases and conditions that caused death. With this results, we suggested some strategy to improve the correctness of death certificate writing practice.

인진 추출물이 마우스 대식세포주인 RAW264.7 세포주의 iNOS 발현 및 superoxide 형성에 미치는 영향

박진상,文錫哉,文九,元秦喜 한국전통의학연구소 1999 한국전통의학지 Vol.9 No.1

Nitric oxide(NO) is synthesized via the oxidation of L-arginine by a family of nitric oxide synthases(NOS), which are either constitutive(cNOS) or inducible(iNOS). The induction of iNOS in tissues can lead to the sustained production of high concentrations of NO which may exert pro-inflammatory effects including vasodilation, edema, cyototoxicity, and its activity can be mediated by various pro-inflammatory cytokine, including interferon γ(INF-γ), tumor necrosis factor, IL-1 and IL-6. The enzyme, iNOS, became a new target for pharmacologcal research with the aim to find new substances for the treatment of chronic inflammatory disorders. Murine macrophages produce large amounts of NO when activated with TEN-γ plus LPS. Murine macrophage-like cell line, RAW 264.7, is a suitable cell model to perform in vitro studies regarding the iNOS system. Artemisin feddei Lev. et Vnt.(Compositae) is a perennial herb growing in Korea. The aerial parts have been used in foik medicine as antiinflammatory, antipyretic, choleretic and diuretic agent. Sesquiterpenelactones were isolated from this plant. In the course of screening for NO inhibitory activity from medicnial plants, the aqueous extract of this plant was found to have a significant activity.