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조성환,손화영,김혜성,최종윤,류시윤,박배근,윤원기,홍성혁,김덕환 충남대학교 수의과대학 동물의과학연구소 2002 動物醫科學硏究誌 Vol.10 No.-
Pilomatricoma was histopathologically diagnosed in a 2.5-year-old male Alaskan malamute. The patient was referred to Chungnam National University, Veterinary Medical Teaching Hospital with solitary, firm and well-circumscribed mass at shoulder. Grossly, the mass was dome shaped to tumor like and 10 x 8 x 5㎝ in size. Some lesions had gritty or bony consistency and was chalky when sectioned Histopathologic feature included mumerous clusters of tightly arranged, basaloid cells with evenly distributed chromatin and ghost cells were characterized by a central unstained zone. The ghost cells often become calcified and osseous metaplasia was also present.
Choi, Yun-Hyeok,Yoo, Hee-Jung,Noh, Ill Chan,Lee, Jeong-Min,Park, Jae Won,Choi, Wahn Soo,Choi, Jung Ho 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.5
The inhibition of Interleukin-1beta (IL-$1{\beta}$) is of substantial interest for the treatment of rheumatoid arthritis. Using an in vitro assay with RAW 264.7 cells, oxo-acetic acid 2-ethoxy-4-(3-hydroxy-2-oxopropyl) phenyl ester (1) was isolated from the roots of Paeonia suffruticosa Andrews as an inhibitor of IL-$1{\beta}$ with an $IC_{50}$ value of 56 ${\mu}M$. Compound 1 is a novel phenylesteric compound from P. suffruticosa Andrews. Compound 1 was shown to inhibit the production of pro-inflammatory cytokines in RAW 264.7 cells. Thus, a possible new action of novel compound is provided explaining the anti-rheumatoid arthritic properties of P. suffruticosa Andrews.
Production of Bio-Based Isoprene by the Mevalonate Pathway Cassette in Ralstonia eutropha
( Hyeok-won Lee ),( Jung-ho Park ),( Hee-seok Lee ),( Wonho Choi ),( Sung-hwa Seo ),( Irika Devi Anggraini ),( Eui-sung Choi ),( Hong-weon Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.10
Isoprene has the potential to replace some petroleum-based chemicals and can be produced through biological systems using renewable carbon sources. Ralstonia eutropha can produce value-added compounds, including intracellular polyhydroxyalkanoate (PHA) through fatty acid and lipid metabolism. In the present study, we engineered strains of R. eutropha H16 and examined the strains for isoprene production. We optimized codons of all the genes involved in isoprene synthesis by the mevalonate pathway and manipulated the promoter regions using pLac and pJ5 elements. Our results showed that isoprene productivity was higher using the J5 promoter (1.9 ± 0.24 μg/l) than when using the lac promoter (1.5 ± 0.2 μg/l). Additionally, the use of three J5 promoters was more efficient (3.8 ± 0.18 μg/l) for isoprene production than a one-promoter system, and could be scaled up to a 5-L batch-cultivation from a T-flask culture. Although the isoprene yield obtained in our study was insufficient to meet industrial demands, our study, for the first time, shows that R. eutropha can be modified for efficient isoprene production and lays the foundation for further optimization of the fermentation process.
Choi, Ji Na,Choi, Yun-Hyeok,Lee, Jeong-Min,Noh, Ill Chan,Park, Jae Won,Choi, Wahn Soo,Choi, Jung Ho Taylor Francis Health Sciences 2012 NATURAL PRODUCT RESEARCH Vol.26 No.24
<P>Trachelospermum jasminoides (Apocynaceae) has pharmacological effects that include anti-inflammatory, anti-bacterial and anti-viral activities, which have been observed from various studies. Of these pharmacological effects, the anti-inflammatory capacity of compounds from T. jasminoides is not yet known exactly. In this study, we investigated the compound that can be used for the suppression of lipopolysacchaide (LPS) stimulated inflammatory responses in macrophages among the five isolated compounds. β-sitosterol-β-D-glucoside (1) was found to reduce nitric oxide (NO) production from LPS-induced RAW 264.7 cells the most. In addition, compound 1 strongly inhibited the interleukin 6 (IL-6) activities of stimulated macrophages. Treatment of RAW 264.7 cells with compound 1 reduced secretion of inflammatory elements including tumour necrosis factor - alpha (TNF-α) and interleukin 1 beta (IL-1β). Thus, compound 1 may be a useful candidate for the development of new drugs to treat endotoxemia and inflammation accompanied by the overproduction of NO.</P>
Molecular Gene Cloning, Expression, and Characterization of Bovine Brain Glutamate Dehydrogenase
( Dae Won Kim ),( Won Sik Eum ),( Sang Ho Jang ),( Chang Sik Yoon ),( Young Hoon Kim ),( Soo Hyun Choi ),( Hee Soon Choi ),( So Young Kim ),( Hyeok Yil Kwon ),( Jung Hoon Kang ),( Oh Shin Kwon ),( Sun 생화학분자생물학회 2003 BMB Reports Vol.36 No.6
A cDNA of bovine brain glutamate dehydrogenase (GDH) was isolated from a cDNA library by recombinant PCR. The isolated cDNA has an open-reading frame of 1677 nucleotides, which codes for 559 amino acids. The expression of the recombinant bovine brain GDH enzyme was achieved in E. coli, BL21 (DE3) by using the pET-15b expression vector containing a T7 promoter. The recombinant GDH protein was also purified and characterized. The amino acid sequence was found 90% homologous to the human GDH. The molecular mass of the expressed GDH enzyme was estimated as 50 kDa by SDS-PAGE and Western blot using monoclonal antibodies against bovine brain GDH. The kinetic parameters of the expressed recombinant GDH enzymes were quite similar to those of the purified bovine brain GDH. The L_(nm) and V_(max) values for NAD^(+) were 0.1 mM and 1.08 μmol/min/mg, respectively. The catalytic activities of the recombinant GDH enzymes were inhibited by ATP in a concentration-dependent manner over the range of 10 - 100 μM, whereas, ADP increased the enzyme activity up to 2.3-fold. These results indicate that the recombinant-expressed bovine brain GDH that is produced has biochemical properties that are very similar to those of the purified GDH enzyme.
Inactivation of Brain myo-Inositol Monophosphate Phosphatase by Pyridoxal-5`-Phosphate
( Dae Won Kim ),( Joung Woo Hong ),( Won Sik Eum ),( Hee Soon Choi ),( Soo Hyun Choi ),( So Young Kim ),( Byung Ryong Lee ),( Jae Jin An ),( Sun Hwa Lee ),( Seung Ree Lee ),( Oh Shin Kwon ),( Hyeok Yi 생화학분자생물학회 2005 BMB Reports Vol.38 No.1
Myo-inositol monophosphate phosphatase (IMPP) is a key enzyme in the phosphoinositide cell-signaling system. This study found that incubating the 1MPP from a porcine brain with pyridoxal-5`-phosphate (PLP) resulted in a time-dependent enzymatic inactivation. Spectral evidence showed that the inactivation proceeds via the formation of a Schiff`s base with the amino groups of the enzyme. After the sodium borohydride reduction of the inactivated enzyme, it was observed that 1.8 mol phosphopyridoxyl residues per mole of the enzyme dimer were incorporated. The substrate, myo-inositol-l-phosphate, protected the enzyme against inactivation by PLP. After tryptic digestion of the enzyme modified with PLP, a radioactive peptide absorbing at 210 nm was isolated by reverse-phase HPLC. Amino acid sequencing of the peptide identified a portion of the PLP-binding site as being the region containing the sequence L-Q-V-S-Q-Q-E-D-I-T-X, where X indicates that phenylthiohydantoin amino acid could not be assigned. However, the result of amino acid composition of the peptide indicated that the missing residue could be designated as a phosphopyridoxyl lysine. This suggests that the catalytic function of IMPP is modulated by the binding of PLP to a specific lysyl residue at or near its substrate-binding site of the protein.
Metaplastic Meningioma Overspreading the Cerebral Convexity
( Yun-hyeok Choi ),( Chan-young Choi ),( Chae-heuck Lee ),( Hae-won Koo ),( Sun-hee Chang ) 대한뇌종양학회 대한신경종양학회 2018 Brain Tumor Research and Treatment Vol.6 No.2
Meningioma is relatively common, benign, and extra-axial tumor accounting for about 20% of primary brain and spinal cord tumors. The World Health Organization (WHO) classified these tumors into Grade I (benign), Grade II (atypical), and Grade III (anaplastic) meningioma. Grade I meningioma which is slowly growing tumor and have some rare subtypes. Among them, metaplastic subtype is defined as a tumor containing focal or widespread mesenchymal components including osseous, cartilaginous, lipomatous, myxoid or xanthomatous tissue, singly or in combinations. We report a rare metaplastic meningioma overspreading nearly whole cerebral convexity from main extra-axial tumor bulk in the parietal lobe.
Lee, Hyeok-Won,Park, Jung-Ho,Lee, Hee-Suk,Kim, Chun-Suk,Lee, Jin-Gyeom,Kim, Won-kyo,Ryu, Kyoung-Hwa,Ahn, Jung-Oh,Lee, Eun-Gyo,Kim, Seon-Won,Jeon, Jong-Min,Yang, Yung-Hun,Choi, Eui-Sung,Lee, Hong-Weon Society for Bioscience and Bioengineering, Japan 2019 Journal of bioscience and bioengineering Vol. No.
<P>Many volatile compounds, such as isoprene, a precursor used in the synthesis of natural rubber, have been produced through fermentation using genetically engineered microorganisms. Despite this biotechnological success, measuring the concentrations of volatile compounds during fermentation is difficult because of their high volatility. In current systems, off-line analytical methods usually lead to product loss, whereas on-line methods raise the production cost due to the requirement of complex devices. Here, we developed a novel on-line gas chromatography (GC)-based system for analyzing the concentration of isoprene with the aim to minimize the cost and requirement for devices as compared to current strategies. In this system, a programmable logic controller is used to combine conventional GC with a syringe pump module (SPM) directly connected to the exhaust pipe of the fermentor, and isoprene-containing samples are continuously pumped from the SPM into the GC using an air cylinder recycle stream. We showed that this novel system enables isoprene analysis during fermentation with convenient equipment and without the requirement of an expensive desorption tube. Furthermore, this system may be extended to the detection of other volatile organic compounds in fermentation or chemical processes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This system analyzes the concentration of isoprene in aerobic fermentation. </LI> <LI> PLC is used to combine conventional GC with SPM directly connected to fermenter. </LI> <LI> Produced isoprene is continuously analyzed using the GC. </LI> <LI> This system may be extended to the detection of other volatile organic compounds. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>