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표고골목에서 발생한 미기록 Hypoxylon sp., 회색버짐버섯(가칭)의 특성
박원철,이봉훈 한국균학회 2002 韓國菌學會誌 Vol.30 No.2
An unrecorded Hypoxylon sp. was observed on the bed-log of lak-mushroom (Lentinula edodes) in Korea. The stroma of the fungus was formed on the bed-log (Quercus spp.) damaged by Korean squirrel (Sciurus vulgaris coreae). Perithecia were 200∼400㎛, and asci containing 8 ascospores wre 7∼8×150∼230㎛ in size. Paraphysis was cylindrical shape. Ascospores were 5∼9×12∼17.5㎛ in size, pale brown to dark brown, elliptical, and smooth. They have germination cleft 1-2 oil droplets.
Won-Chull Bak,Bong-Hun Lee,Young-Ae Park,Hyun-Seok Kim 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4
A harmful fungus occurred seriously in bed-log of shiitake(Lentinula edodes) in Jangheung-Gun, Korea. The fungus was identified as Bjerkandera adusta by its morphology and ITS(Internal Transcribed Spacer) analysis. The fungus was reported as causal agent of stem-rot of Populus euramericana in Korea, but not reported in bed-log of shiitake until this notification. Thus, studies were made to investigate inside condition of bed-log of shiitake damaged by B. adusta, physiological characteristics of B. adusta and antagonism between these two fungi. First of all, B. adusta is white-rotting fungus like shiitake and wood-rotting condition is similar to that of shiitake. But, there are a lot of small spots in damaged wood tissue under bark which are not seen in case of shiitake. Optimal temperature for mycelial growth of B. adusta is ca. 30℃ while that of shiitake is ca. 25 ℃. When confrontation cultures were made between these two fungi under 15℃, 20℃, 25℃ and 30℃, B. adusta has antagonistic ability against shiitake in all the temperatures. From the results of experiments, if the bed-logs of shiitake are exposed to high temperature, there should be mass propagation of B. adusta, and shiitake mycelia will be seriously injured by the fungus. Therefore, to prevent the damage by B. adusta, it is needed to grow the mycelia of shiitake fast in the bed-log, and to avoid exposure of the bed-log to high temperature in summer.
( Won Chull Bak ),( Ji Heon Park ),( Young Ae Park ),( Kang Hyeon Ka ) 한국균학회 2014 Mycobiology Vol.42 No.3
Shiitake mushrooms (Lentinula edodes) containing β-glucans may be beneficial for human health; they have been used in the treatment of cancer, hypertension, and high cholesterol levels. The objective of this study was to determine the β-glucan content in different sections of the fruiting bodies and mycelia of ten shiitake mushroom cultivars. The measured β-glucan content ranged from 20.06 ± 1.76% to 44.21 ± 0.13% in the pileus sections, and from 29.74 ± 1.40% to 56.47 ± 4.72% in the stipe sections. The results of this study indicate that the variance in β-glucan content dependent on the shiitake cultivar, and that the β-glucan content is higher in the stipe than in the pileus.
선발육종 (選拔育種) 및 교잡육종에 (交雜育種) 의한 원목재배용 표고균주 육성
박원철(Won Chull Bak),이태수(Tai Soo Lee),이원규(Won Kyu Lee),변병호(Byung Ho Byun),이창근(Chang Keun Yi) 한국산림과학회 1996 한국산림과학회지 Vol.85 No.2
Selective breeding and hybridization were attempted to improve shiitake(Lentinus edodes) strains for bed-log cultivation, and the results obtained are as follows. 1. Thirty five strains collected in Korea and abroad were cultivated for one generation(five years) and. fruit body yields were estimated by the amounts produced from 1㎥ of oak tree(Quercus mongolicai logs. Thus, three high-temperature type strains including FRI 177 showing productivity of 139㎏/㎥log, two mid-temperature type strains including FRI 188 of 120㎏/㎥log, and seven low-temperature type strains including FRI 187 of 96㎏/㎥log were selected as superior strains. 2. Twelve hybrid strains confirmed as independent strains through indoor-test with hybrid strains made by Di-mon mating method were cultivated for five years(one generation). Among them, FRI 182 and FRI 181 were turned out to be superior strains. FRI 182 of high-temperature type showed the yield of 108㎏ per 1㎥logs and FRI 184 of low-temperature type produced 103㎏/㎥log. 3. There were no significant differences in productivity among high-, mid- and low-temperature types, but some differences appeared among strain groups. The correlation was not shown in productivities between bed-log and sawdust cultivations.
Berberine Sulphate 를 이용한 형광현미경기법에 의한 수목마이코플라스마검정
박원철(Won Chull Bak),나용준(Yong Joon La) 한국산림과학회 1991 한국산림과학회지 Vol.80 No.2
The efficacy of berberine sulphate, a fluorochrome having binding properties with both DNA and RICA, was investigated for the detection of mycoplasma-like organisms(MLOs) in jujube(Zizyplzus jujuba), paulownia (Paulounia tomentosa), mulberry(Morus alba) trees and periwinkle (Catharantlaus roseus) plant. When examined under fluorescence microscope, berberine sulphate-stained sections of diseased samples showed distinct MLO-specific fluorescent particles in the phloem area, while such fluorescence was absent in the healthy ones. This staining technique was proved to be a very accurate method for the diagnosis of MLO infections in woody and herbaceous plants. Furthermore, the cheap and easy procedure could be used to test a, great number of samples on MLO infections with reliability and rapidity.
박원철 ( Won Chull Bak ),박영애 ( Young Ae Park ),이봉훈 ( Bong Hun Lee ),가강현 ( Kang Hyeon Ka ),박지헌 ( Ji Heon Park ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.1
New Shiitake (Lentinula edodes) strain “Chunbaegko” was bred by Di-mon method. Bed-log cultivation of “Chunbaegko” was performed. Fruit-body production of “Chunbaegko” was most at spring and autumn. Optimal temperature of fruit-body formation was 14~22oC Baegwhako, the best quality fruit-body, is produced during spring. The diameter of pileus is ca. 52 mm. The total amount of fruit-body production during 4 years(one generation) was 140 kg/m3 log.
우리나라 표고의 생장 , 부후특성 (腐朽特性) 및 톱밥배양에 관한 연구
박원철(Won Chull Bak),이은영(Eun Young Lee),윤갑희(Kab He Yoon),이원규(Won Kyu Lee),이창근(Chang Keun Yi),홍기성(Ki Sung Hong) 한국산림과학회 1994 한국산림과학회지 Vol.83 No.1
Studies were made to find out the mycelial growth and rotting characteristics of 91 Lentinus edodes isolates collected or hybridized in Korea and abroad, and their adaptability to bag-culture. The results are as follows. 1. There were little differences in mycelial growth at PDA and oak (Quercus mongolica) sawdust-based medium, and rotting among high-, mid- and low temperature types of L. edodes isolates. But. the fruit yield of high-temperature type was much higher than those of the other two types. The fruit yields of the isolates in the same strain group were apparently different. 2. From the sawdust-based culture of 91 isolates, FRI 221, FRI 208 and FRI 169 were selected as excellent strains in yield and Quality, showing fruiting of 157g, 152g and 119g per 1㎏-medium, respectively. 3. Attempts to culture in various media with different substrates resulted in almost same fruit yields, and the yield was proportional to medium weights as 2.5㎏-medium showed 2.5 times more yield than 1㎏ -medium. Fruit yields were different according to incubation periods and the period of little more than 100days was best. 4. When the correlation analyses among mycelial growth, rotting ability, yield and fruit-shape normality were made with the 91 isolates, mycelial growth at sawdust-based medium showed highly positive correlation with that at PDA, while fruit yield was negatively correlated with mycelial growth in PDA and sawdust-based medium.