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Expression and characterization of transmembrane and coiled-coil domain family 3
( Wern-joo Sohn ),( Jae-young Kim ),( Dongbum Kim ),( Jeong-a Park ),( Younghee Lee ),( Hyung-joo Kwon ) 생화학분자생물학회(구 한국생화학분자생물학회) 2016 BMB Reports Vol.49 No.11
Transmembrane and coiled-coil domain family 3 (TMCC3) has been reported to be expressed in the human brain; however, its function is still unknown. Here, we found that expression of TMCC3 is higher in human whole brain, testis and spinal cord compared to other human tissues. TMCC3 was expressed in mouse developing hind brain, lung, kidney and somites, with strongest expression in the mesenchyme of developing tongue. By expression of recombinant TMCC3 and its deletion mutants, we found that TMCC3 proteins self-assemble to oligomerize. Immunostaining and confocal microscopy data revealed that TMCC3 proteins are localized in endoplasmic reticulum through transmembrane domains. Based on immunoprecipitation and mass spectroscopy data, TMCC3 proteins associate with TMCC3 and 14-3-3 proteins. This supports the idea that TMCC3 proteins form oligomers and that 14-3-3 may be involved in the function of TMCC3. Taken together, these results may be useful for better understanding of uncharacterized function of TMCC3. [BMB Reports 2016; 49(11): 629-634]
Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation.
Sohn, Wern-Joo,Jung, Hye-In,Choi, Min-A,Han, Jin-Hyun,Gwon, Gi-Jeong,Yamamoto, Hitoshi,Lee, Sanggyu,Ryoo, Zae Young,Park, Eui-Kyun,Shin, Hong-In,Jung, Han-Sung,Kim, Jae-Young Springer 2011 Cell and tissue research Vol.345 No.2
<P>The molecular mechanisms for epithelial differentiation have been studied by observing skin development in embryogenesis, but the early signaling modulations involved in tongue epithelial differentiation are not completely understood. Based on the gene expression patterns of the Fgf signaling molecules and previous results from Fgf10 and Fgfr2b knockout mice, it was hypothesized that there would be fundamental signaling interactions through the epithelial Fgfr2b and its mesenchymal ligand Fgf10 to regulate tongue epithelium differentiation. To elucidate these reciprocal interactions in tongue epithelial differentiation, this study employed an in vitro tongue organ culture system with antisense-oligodeoxynucleotides (AS-ODNs) and recombinant protein-soaked bead implantation for the loss-of-function and gain-of-function studies. Functional analysis of Fgf signaling revealed precise reciprocal interactions, which showed that mesenchymal Fgf10 rather than Fgf7 modulates tongue epithelial differentiation via Fgfr2b in a temporal- and spatial-specific manner.</P>
Morphological evidences in circumvallate papilla and von Ebners' gland development in mice
Wern-Joo Sohn,Gi-Jeong Gwon,Chang-Hyeon An,Cheil Moon,Yong-Chul Bae,Hitoshi Yamamoto,Sanggyu Lee,Jae-Young Kim 대한해부학회 2011 Anatomy & Cell Biology Vol.44 No.4
In rodents the circumvallate papilla (CVP) with its underlying minor salivary gland the von Ebners' gland (VEG) is located on the dorsal surface of the posterior tongue. Detailed morphological processes to form the proper structure of CVP and VEG have not been properly elucidated. In particular the specific localization patterns of taste buds in CVP and the branching formation of VEG have not yet been elucidated. To understand the developmental mechanisms underlying CVP and VEG formation detailed histological observations of CVP and VEG were examined using a three-dimensional computeraided reconstruction method with serial histological sections and pan-Cytokeratins immunostainings. In addition to defi ne the developmental processes in CVP and VEG formation we examined nerve innervations and cell proliferation using microinjections of AM1-43 and immunostainings with various markers including phosphoinositide 3-kinase Ki-67 PGP9.5 and Ulex europaeus agglutinin 1 (UEA1). Results revealed specific morphogenesis of CVP and VEG with nerve innervations patterns evaluated by the coincided localization patterns of AM1-43 and UEA1. Based on these morphological and immunohistochemical results we suggest that nerve innervations and cell proliferations play important roles in the positioning of taste buds in CVP and branching morphogenesis of VEG in tongue development.
CpG-oligodeoxynucleotide protects immune cells from γ-irradiation-induced cell death
Sohn, Wern-Joo,Lee, Keun-Wook,Choi, Soo Young,Chung, Eunkyung,Lee, Younghee,Kim, Tae Yoon,Lee, Suk Kyeong,Choe, Yong-Kyoung,Lee, Jeung-Hoon,Kim, Doo-Sik,Kwon, Hyung-Joo Elsevier 2006 Molecular immunology Vol.43 No.8
<P><B>Abstract</B></P><P>Synthetic oligodeoxynucleotides (CpG-ODNs) and bacterial DNA containing unmethylated CpG dinucleotides in the context of particular base sequences (CpG motifs) are known to inhibit anti-IgM-induced growth arrest and apoptosis of WHEI 231 B lymphocytes, and spontaneous apoptosis of mature spleen B cells in a sequence-specific fashion of the CpG-ODN. Here we report that CpG-ODN protects from the cell death induced by γ-irradiation of primary mouse spleen cells as well as mouse RAW 264.7 macrophage cells and human RPMI 8226 B cells. Experimental results showed that CpG-ODN promotes growth of the cells, and protects the cells from γ-irradiation-induced cell death accompanying Bcl-xS/L and Bcl-2 upregulation. Furthermore, survival of macrophages was enhanced when splenocytes were pretreated with CpG-ODN. Our results suggest the potential application of CpG-ODNs for more efficient cancer radiotherapy by enhancing survival of normal immune cells after radiation damage.</P>