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( Jung Joon Min ),( Uyen Chi N Le ),( Ho Jae Han ),( Hyun Suk Lee ),( Sung Min Moon ),( Jung Sun Heo ),( Yun Jung Lee ),( Kyu Youn Ahn ),( Kwang Sung Park ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4
The detection of implanted stem cells in vivo almost always depends on postmortem histology. In this study, we sought to demonstrate the feasibility of adenovirus-mediated transient expression of the firefly luciferase(Fluc) reporter gene and longitudinal monitoring of implanted embryonic stem(ES) cells in rat corpus cavernosum by optical bioluminescence. Mouse ES cells(ES-E14TG2a) were transiently transfected with Ad-CMVFluc( MOI=100) and implanted into the corpus cavernosum of male Sprague-Dawley rats that were derived into the following groups: sham operated controls and injection of 5 different doses of ES cells(1×103, 1×104, 1×105, 1×106 and 5×106). Cell survival was assessed histologically and by bioluminescence imaging every other day after cellular implantation. All injected rats with cell numbers over 1×104 revealed the bioluminescence in the genital area until terminating the experiment(for 2 weeks), and histology showed increased smooth muscle contents compared to control rats. The study demonstrated a feasibility of adenovirus-mediated transduction of an imaging reporter gene applied for optical imaging of ES cells. The location, magnitude and survival of ES cells in rat corpus cavernosum can be monitored noninvasively with bioluminescence imaging system.