Cellular and Histological Differences between ACL Bundles of Goat Knee

( Ho Lee1 ),( Tomoyuki Matsumoto ),( Masahiro Nozaki ),( Sheila J M Ingham ),( Freddie H Fu ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4

We aimed to investigate whether there exist the differences in the cellular properties and histological structures between AM and PL bundle of goat ACL. ACL tissues obtained from goat knees were divided to two experiments: tissue explant culture and histological assessment. AM bundle was divided into 3(proximal, middle, and distal) and PL into 2(proximal, distal) zones according to the length for AM and PL bundle. In the tissue explant culture, ACL cells demonstrated 2 different cell types which consist of spindle shaped fibroblasts and ovoid shaped fibrochondrocytes and three different cellular outgrowth patterns. The percentage of fibrochondrocytic pattern of AM bundle was significantly higher than that of PL bundle. In the section divided analysis of AM, the rate of fibrochondrocytic pattern in the mid portion was significantly lower than that in the proximal and distal portion. In the histological evaluation, we identified 3 distinguishable bundles divided by the septum, AM, PL, and intermediate( IM), and two different types of cells, fibroblasts and fibrochondrocytes, in all portions of AM and PL. The number of fibrochondrocytes stained by toluidine blue in AM bundle was larger than that in PL bundle. There was no difference in the cell density between AM and PL bundle of ACL, whereas the crimp length was longer in AM bundle compared to PL bundle. Our finding demonstrated that the goat ACL had the differences in cellular morphology, outgrowth pattern, and histological structure between AM and PL bundle of ACL.

Sound Distance Localization Using Head-Related Transfer Functions (HRTFs) Approximated by IIR Filters

Hiroyuki TSUCHIYA,Tomoyuki NISSATO,Hiroshi HASEGAWA,Shuichi MATSUMOTO,Atsushi KOIKE,Koichi TAKAGI,Masao KASUGA 대한전자공학회 2003 ITC-CSCC :International Technical Conference on Ci Vol.2003 No.7

Analysis of Non-Evaporating Spray Characteristics from Jatropha Methyl Ester and its Blends

( Annisa Bhikuning ),( Naoki Matsumoto ),( Tomoyuki Mukayama ),( Eriko Matsumura ),( Jiro Senda ) 한국액체미립화학회 2017 한국액체미립화학회 학술강연회 논문집 Vol.2017 No.-

Biodiesel or Methyl ester is an alternative fuel that is produced from edible and non-edible vegetable oils. Jatropha is one of the promising fuels that can be substitute from diesel fuel in the future. Jatropha methyl ester is produced from non-edible vegetable oil (Jatropha tree). Therefore, Jatropha methyl ester can be acceptable to replace the diesel fuel without make any disturb for the food market in the world. Jatropha trees can be found and easily grown in tropical regions in the world such as Indonesia, Malaysia and India. Tridecane (C13H28) is an alkane hydrocarbon. It is colorless and combustible. The blending of Jatropha methyl ester with tridecane would make change the chemical properties of Jatropha itself. Study in non-evaporating spray characteristics is important in order to understand the spray behavior for biodiesel and its blends. In this paper, Jatropha methyl ester is blended with tridecane in composition of 25% (Jatropha Methyl Ester 25% and tridecane 75%), 50% (Jatropha Methyl Ester 50% and tridecane 50%) and 75% (Jatropha Methyl Ester 75% and tridecane 25%). Non evaporating spray characteristics for diesel sprays provides parameter such as spray tip penetration, spray cone angle and droplet size. However, before spray estimation are led, the conditions inside the vessel are in good condition. In this study, the ambient pressure was 2 MPa and the injection pressures were 50,100 and 150 MPa. The spray tip penetration length is analyzed by using high speed camera and some properties of Jatropha methyl ester blends were analyzed. It is shown that, the correlation between the surface tension, viscosity and density with longer or narrower of spray tip penetration and cone angle can be summarized. Furthermore, the correlations between the blending of Jatropha Methyl Ester and tridecane composition is also affected to the spray tip penetration and cone angle.

Effects of Soil Amendment on Cadmium and Arsenic Concentration and Arsenic Speciation in Rice Grain

Tomohito Arao,Akira Kawasaki,Koji Baba,Shingo Matsumoto,Tomoyuki Makino 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Second-Look Arthroscopic Findings and Clinical Outcomes after Adipose-Derived Regenerative Cell Injection in Knee Osteoarthritis

Yuma Onoi,Takafumi Hiranaka,Yuichi Hida,Takaaki Fujishiro,Koji Okamoto,Tomoyuki Matsumoto,Ryosuke Kuroda 대한정형외과학회 2022 Clinics in Orthopedic Surgery Vol.14 No.3

Background: To evaluate the clinical outcomes and second-look arthroscopic findings after intra-articular adipose-derived regenerative cell (ADRC) injection as treatment for knee osteoarthritis (OA). Methods: ADRCs were administered to 11 patients (19 knees; mean age, 61.7 years) with knee OA. Subcutaneous adipose tissue was harvested by liposuction from both thighs, and arthroscopic lavage was performed, followed by ADRC injection (mean dose, 1.40 × 107 cells) into the synovial fluid. Outcome measures included the Knee Injury and Osteoarthritis Outcome Score, Lysholm score, and visual analog scale score. Arthroscopic examinations were performed to assess the International Cartilage Repair Society cartilage injury grade preoperatively and overall repair postoperatively. Noninvasive assessments were performed at baseline and at 1-, 3-, and 6-month follow-ups; arthroscopic assessments were performed at baseline and at 6 months. Results: All outcome measures significantly improved after treatment. This improvement was evident 1 month after treatment and was sustained until the 6-month follow-up. Data from second-look arthroscopy showed better repair in low-grade cartilage lesions than in lesions with a greater degree of damage. No patients demonstrated worsening of Kellgren-Lawrence grade, and none underwent total knee arthroplasty during this period. Conclusions: Clinical outcomes were improved in patients with knee OA after ADRC administration. Cartilage regeneration was more effective in smaller damaged lesions than in bigger lesions.

Differences in preoperative planning for high-tibial osteotomy between the standing and supine positions

( Takehiko Matsushita ),( Shu Watanabe ),( Daisuke Araki ),( Kanto Nagai ),( Yuichi Hoshino ),( Noriyuki Kanzaki ),( Tomoyuki Matsumoto ),( Takahiro Niikura ),( Ryosuke Kuroda ) 대한슬관절학회 2021 대한슬관절학회지 Vol.33 No.-

Introduction: Previous studies have reported that alignment changes depend on the patient’s position in orthopedic surgery. However, it has not yet been well examined how the patient’s position affects the preoperative planning in high-tibial osteotomy (HTO). Therefore, the aim of this study was to investigate the effects of the patient’s position on preoperative planning in HTO. Materials and methods: A total of 60 knees in 55 patients who underwent HTO were retrospectively examined. Virtual preoperative planning for medial open-wedge HTO (OWHTO), lateral closed-wedge HTO (CWHTO), and hybrid CWHTO were performed by setting the percentage of the weight-bearing line (%WBL) at 62% as an optimal alignment. The correction angle differences between the supine and standing radiographs were measured. The virtual %WBL (v%WBL) was determined by applying the correction angle obtained from the standing radiograph to the supine radiograph. The %WBL discrepancy (%WBLd) was calculated as v%WBL - 62 (%) to predict the possible correction errors during surgeries. A single regression analysis was performed to examine the correlation between the correction angle difference and %WBLd. Results: The mean correction angle was significantly higher when the preoperative planning was based on standing radiographs than when based on supine radiographs (P < 0.001), and the mean difference was 2.2 ± 1.5°. The difference between the two conditions in the medial opening gaps for OWHTO, lateral wedge sizes (mm) for CWHTO, and hybrid CWHTO were 2.6 ± 2.0, 2.3 ± 1.6, and 1.9 ± 1.4, respectively. The mean v%WBL was 71.2% ± 7.3%, and the mean %WBLd was 10.1% ± 7.4%. A single regression analysis revealed a linear correlation between the correction angle difference and %WBLd (%WBLd = 4.72 × correction angle difference + 0.08). No statistically significant difference in the parameters was found between the supine and standing radiographs postoperatively. Conclusions: We found significant differences in the estimated correction angles between the supine and standing radiographs in the planning for HTO. Therefore, surgeons should carefully consider the difference between supine and standing radiographs and estimate the possible correction error during surgery when planning a HTO.

Analysis of popliteal artery location for high tibial and distal tuberosity osteotomy using contrast-enhanced computed tomography

( Akiyoshi Mori ),( Takehiko Matsushita ),( Nobuaki Miyaji ),( Kanto Nagai ),( Daisuke Araki ),( Noriyuki Kanzaki ),( Tomoyuki Matsumoto ),( Takahiro Niikura ),( Yuichi Hoshino ),( Ryosuke Kuroda ) 대한슬관절학회 2022 대한슬관절학회지 Vol.34 No.-

Background: Our objective was to evaluate the location of popliteal artery (PA) in osteotomy planes during high tibial osteotomy (HTO) and to determine a safer angle for screw drilling to the tibial tuberosity during distal tuberosity osteotomy (DTO). Methods: Twenty knees in 20 patients who underwent contrast-enhanced computed tomography for cardiovascular diseases were examined. Osteotomy planes for open-wedge HTO (OWHTO) and hybrid closed-wedge HTO (hybrid CWHTO) were created using three-dimensional bone models. The distance from the posterior cortex of the tibia to the PA (dPC-PA) in the osteotomy planes was measured in the virtual osteotomy planes. The dangerous point (Point D1) was defined as the point 17.5 mm away from PA, setting the working length of the bone saw as 35 mm. The distance between the most medial point of the tibial cortex (Point M) and Point D1 in OWHTO and the most lateral point (Point L) and Point D1 in hybrid CWHTO were examined (dM-D1 and dL-D1, respectively). The location of Point D1 to the osteotomy line (%D1) was expressed as percentage, setting the start and end of the osteotomy line as 0% and 100%, respectively. To determine the safe angle for screw drilling in DTO, the angle between the line tangential to the medial cortex of the tibia and that passing through the center of the tibial tuberosity and PA were measured. Results: In OWHTO and hybrid CWHTO, the mean dPC-PA was 10.6 mm (6.9-16.5 mm) and 10.2 mm (7.3-15.4 mm), respectively. The mean dM-D1 in OWHTO was 25.9 mm (24.6-27.2 mm) and dL-D1 in hybrid CWHTO was 5.1 mm (2.9-7.4 mm). The mean %D1 was 47.6 ± 3.7% in OWHTO and 9.3 ± 4.1% in hybrid CWHTO, respectively. The minimal angle between the two lines in DTO was 35.2°. Conclusion: PAs could run within 10 mm from the posterior cortex in the osteotomy planes of HTO. Therefore, proper posterior protection is necessary when cutting posterior cortex. An angle of less than 35° against the medial cortex line would be safe for screw fixation to avoid vascular injury in DTO.