151종 생약제 추출물이 B형 간염 바이러스 표면 항원(HBsAg)과 항체(HBsAb)의 결합반응 그리고 HBV DNA polymerase 활성에 미치는 효과 : 한국산 각종 생약제를 이용한 B형 간염치료제의 개발

정태호,김정철,김문규,이인선,채성철,김승래,정준모,이인수,김승호,함경수 慶北大學校 醫科大學 1992 慶北醫大誌 Vol.33 No.3

세계인구 43억명 중 B형 간염 바이러스 보균자는 2억 1천 5백만명이며 이중 78%인 1억 6천만명 이상이 아시아 대륙에 집중되어 있다. 한국도 B형 바이러스 간염의 침윤지대이다. 본 연구는 B형 바이러스 보균자 혹은 만성간염을 치료할 수 있는 생약제를 탐색할 목적의 일환으로 한국에서 시판하는 생약제 151종을 구입하여 증류수로 추출하고 여과한 후 냉동건조하여 HBs 항원에 대한 결합능, HBV DNA polymerase억제능, tumor necrosis factor(TNF) 유리에 미치는 영향, 그리고 galactosamine 유발 간염에 대한간장보호 작용을 조사하였다. 151종의 생약제중 0.16㎍/㎖ 농도에서 간염 vaccine 내에 함유된 HBs 항원과 결합하는 생약제는 지유, 가자, 복분자, 대황, 정향, 빈량, 산수유, 소목, 마황, 쇄양, 후박, 목과, 양강, 오약, 산사, 목단피 등 16종이었다. 이들 생약제중 대황(Rheum palmatum L. 大黃), 가자(Terminalia chebula R. 訶子), 지유(Sanguisorba officinalis L. 地楡), 복분자(Rubus coreanus M. 覆盆子)는 HBV DNA polymerase 활성도를 비교적 강하게 억제하였으며 빈량(Areca catechu L. 빈랑), 정향(Eugenia caryophyllata T. 丁香), 목과(Chaenomeles japonica L. 木瓜), 산수유(Cornus officinalis S. 山茱萸)는 약하게나마 HBV DNA polymerase 활성을 억제하였다. 또한 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 대상으로 TNF 유리에 미치는 영향을 조사한 결과 소목을 제외한 8종의 생약제 모두가 대식세포를 자극하여 TNF를 유리시켰다. 그리고 장차 임상에 사용함을 전제로 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 galactosamine 유발 간염(백서)에 투여하여 간기능 검사에 미치는 효과를 조사하였으나 간기능 검사상으로는 특별한 보호작용을 나타내지 못하였으며 생약제 단독 투여로서는 간기능에 대하여 나쁜 영향을 주지 않았다. Hepatitis B virus infection is major cause of acute and chrome hepatitis, cirrhosis of liver, and hepatocellular carcinoma. There are over 200 million carriers of hepatitis B virus in the world, nearly 80 % of whom live in the South East Asia, Korea is also highly endemic area of hepatitis B virus infection. This study was performed to evaluate the effect of extracts of traditional Korean herbal medicines on hepatitis B virus. 151 traditional herbs were extracted, filtered and lyophilized. Using 151 herbal extracts we tested binding capability to the HBs Ag, inhibition of HBV DNA polymerase, release of tumor necrosis factor (TNF), and protection against galactosamine induced liver damage. Among 151 herbs, 16 herbs (Sanguisorba officinalis L., Terminalia chebula R., rubus coreanus M., Rheum palmatum L., Eugenia caryophyllata T., Areca catechu L., Cornus officinalis S., Caesalpinia sappan L, Ephedra sinica S., Cynomorium songaricum R., Machilus thunbergii S., Chaenomeles japonica L., Alpinia officinarum H., Lindera strychnifolia V., Crataegus pinnatifida B., Paeonia suffruticasa A. ) had binding capability to HBs Ag which contained in the commercial hepatitis vaccine, at 16 ug/㎖ of concentratioa Among the above 16 herbs, Rheum palmatum L., Terminalia chebula R., Sanguisorba officinalis L., Rubus coreanus M. significantly inhibited the HBV DNA polymerase activity, and Areca catechu L., Eugenia caryophyllata T., Ephedra sinica S., Caesalpinia sappan L., Chaenomeles Japonica L., Cornus officinalis S. also inibited the HBV DNA polymerase activity but less effective. All of the herbs, except Caesalpinia sappan L., which inhibited the HBV DNA polymerase activity, actively stimulated the mononuclear cells to release tumor necrosis factor(TNF) in whole blood culture system. The herbs which inhibited the HBV DNA polymerase activity, did not show any protective effect in galactosamine-induced hepatitis in rats, but there was no direct toxic effect when herbs were administered alone.

우관상동맥 개구부 완전 폐쇄가 동반된 대동맥 판상부 협착증 1례

임상현,김종진,김희열,진승원,전희경,노태호,채장성,김재형,홍순조,최규보,윤정섭,김치경 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

B형 간염 바이러스 단백질에 있어서 HLA-A2에 의해 표현되는 Epitope 펩타이드 들의 분석

이희구,임종석,김승목,이기영,김희수,김승호,권태종,최인성,정태화,김길현 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

The cytotoxic T lymphocyte (CTL) are an important component in host defense mechanism against viral infection. They can recongnize virus-derived peptides presented by the Class I MHC molecule at the cell surface of the infected cells. On searching for effective CTL epitopes of hepatitis B virus(HBV), we synthesized a distinct set of 9-10 mer peptide containing amino acid sequence of hepatitis B virus surface protein that are selected on the basis of a computer modeling and the previously described HLA-A2 specific motifs.Binding assay of the synthetic peptides to HLA-A2 molecules using human antigen processing defectant T2 cells showed that 3 out of 4 synthetic peptides enhanced the expression of HLA-A2 molecule on T2 cell surface.Two anchor positions, namely P2 and P9(or P10) appeared to play a decisive role for binding.Structural. characteristics of the peptides addressed by molecular dynamics simulation was analysed and compared.These peptides also partially triggered CTL isolated from human peripheral blood mononuclear cells of HBV positive patients, and the response was peptide-spcific.These results showed that negatively-charged amino acid residue at P2 hampered binding affinity of the peptides to HLA-A2 molecules, and that binding affinity of the peptides are not always reflected by their immunogenicity among natural T cell repertoire.

B형 간염 바이러스 단백질에 있어서 HLA-A2에 의해 표현되는 Epitope 펩타이드 들의 분석

이희구,임종석,김승목,이기영,김희수,김승호,권태종,최인성,정태화,김길현 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-

The cytotoxic T lymphocyte(CTL) are an important component in host defense mechanism against viral infection. They can recongnize virus-derived peptides presented by the ClassⅠ MHC molecule at the cell surface of the infected cells. On searching for effective CTL epitopes of hepatitis B virus(HBV), we synthesized a distinct set of 9-10 mer peptide containing amino acid sequence of hepatitis B virus surface proteion that are selected on the basis of a computer modeling and the previously described HLA-A2 specific motifs. Binding assay of the synthetic peptides to HLA-A2 molecules using human antigen processing defectantn T2 cells showed what 3 out of 4 synthetic peptides enhaced the expression of HLA-A2 molemule on T2 cell surface. Two anchor positions, namely P2 and P9(or P10) appeared to play a decisive role for binding. Structural chacteristics of the peptides addressed by molecular dynamics simulation was analysed and compared. These peptides also parially triggerd CTL isolatied frmo human peripheral blood mononuclear cells of HBV positive patients, and the response was peptide-specific. These results showed that negatively-charged amino acid residue at P2 hampered binding affinity of the peptides to HLA-A2 molecules, and that binding affinity of the peptides are not always reflected by thier immunogenicity among natural T cell repertoire.

PC에 의한 재래식 NC 선방의 DNC화

김근태,정선환,노승훈 金烏工科大學校 1991 論文集 Vol.12 No.-

The key feature of any DNC System is the on-demand supply of parts program data to a number of NC machine tools. Usually DNC Systems are based on the main frame computers or mini computers. This paper describes the design philosophy and the implementation of a PC-based Low-Cost-DNC system of a conventional NC Lathe which was made in the early 1970s.

용담댐 운영계획과 금강유황 및 대청댐 저수량의 변화

김태철,문종필,민진우,박승기 忠南大學校 環境問題硏究所 1997 環境硏究 Vol.15 No.-

Yongdam multipurpose dam is under construction to divert a part of Geum riverflow to Mankyung watershed and to supply the domestic water to the Chunju region and produce the hydro-electricity. Generally, construction of dam by the method of inter-regional water transfer affects the quantity and quality of water in the down streams and reservoirs. The impact of operation plan of Yongdam dam on the quantity and quality of water in the Geum river and Daechung dam was investigated. It was recommended that the discharge of water transfer from one watershed to another should be minimized as much as possible.

분만손상

김태승,황건성,김주학 대한골절학회 2003 대한골절학회지 Vol.16 No.2

서론 : 분만손상이란 진통이나 분만기간 중에 발생될 수 있는 태아에게 해로운 모든 조건을 말한다. 이러한 손상에는 저 산소증으로 인한 뇌손상과, 기계적인 요소에 의한 골절, 신경마비, 두개내 혈종 및 복부손상 등을 포함하고 있다. 분만손상을 야기시킬 수 있는 위험 요인으로는 둔위분만, 감자분만, 아두골반 불균형, 난산, 과체중 등이 있으나 최근에는 산전관리 및 분만방법의 개선, 그리고 제왕절개술의 증가추세로 분만손상율은 감소를 보이고 있다. 정형외과 영역에서의 분만손상은 쇄골 골절, 상완골 골절, 상완 신경총손상, 대퇴골 골절 등이 있으며, 이에 본 병원에서는 최근 11년간 경험한 분만손상에 대해 연구 분석하였다. 대상 및 방법 : 1991년1월부터 2001년 12월까지 한양대의료원 서울병원에서 경험한 분만손상중 두개 내외의 혈종과 뇌신경손상 복부손상을 제외한 사지에 발생된 골절, 신경손상 환자 17명(남자 9명, 여자 8명), 20례를 대상으로 하였다. 두개 내외의 혈종과 뇌신경손상, 복부손상은 제외하였으며, 지료기록지를 조사하여 분만손상에 대한 자료를 얻었다. 결과 : 본 병원에서 경험한 분만손상 중 사지에 발생한 골절, 신경손상은 쇄골 골절 9례, 대퇴골 골절 3례, 상완골 골절 1례, 상완 신경총손상 6례, 원위대퇴골 골단분리증 1례이었다. 쇄골 골절 환아는 재태기간 40-42주사이에 분만되었으며, 신생아의 평균 체중은 4.25 ㎏으로 신생아체중 표준치에 비해 높았으며, 상완 신경총손상 환아 역시 재태기간 40-42주에 분만되었고, 평균 체중 4.42 ㎏으로 높았다. 대퇴골 골절 3례는 모두 둔위로 인한 제왕절개술 분만시에 발생하였고, 이들 중 1례는 좌골부결합체 (ischiopagus)로 태어났다. 원위대퇴골단 분리증 분리손상은 초기에는 간과되었으나, 시간이 경과하면서 현저한 골막반응을 나타내어 통해 분만손상으로 진단되었다. 결론 : 쇄골 골절과 상완 신경총손상은 과체중과 매우 관련이 있다고 할 수 있겠으며, 대퇴골골절은 둔위와 관련하여 제왕절개술시의 분만술기와 관계있다고 할 수 있겠다. 또, 마비증상은 상완 신경총 손상이나 골절로 인한 가성마비로서 나타날 수 있기 때문에 이에 대해 주의깊게 관찰해야 되겠다.무엇보다도 분만손상은 과체중과 관련된 경우가 많으므로, 초음파 검사 또는 산전 진단 방법을 통해 과체중으로 분만되는 것을 지양하여 분만손상을 줄이는 것이 필요하다 하겠다. Purpose : Birth injury is any condition that affects the adversely during the labor of delivery. These traumatic injuries are subdivided further into those from hypoxia and mechanical factors. Risk factor of birth injuries have been reported to breech presentation, forcep delivery, cephalopelvic desproportion dystocia, and high birth weight. But, because of preterm care and development of delivery method and Cesarian section, recently the rate of birth injury was decreasing and the articles about birth injuries in orthopaedic fields have not been much. We analyzed the data about birth injuries occurred during recent 11 years. Materials and Methods : We reviewed the charts and patients the 17 patients (20 cases) of birth injuries including fracture, nerve injury during the delivery at Hanyang University hospital from Jan. 1991 to Dec. 2001. But, we excluded birth injury of head and abdomen. Results : The number of fractures and nerve injuries on extrimities in our hospital were clavicle fracture 9 cases, brachial plexus injury 6 cases, femur fracture 3 cases, separation of distal remoral epiphysis I case, humerus fracture I case. Patients of clavicle fracture were born at mean intrauterine period 41.5 weeks and mean birth weight of the patients was 4.25 ㎏. Patients of brachial plexus injury were born at mean intrauterine period 39.5 weeks and mean birth weight of the patients was 4.42㎏. Three cases of femoral fracture were all due to breech position while cesarean operartion and one of these cases were born were as ishiophagus. We found the distal femoral epiphyseal separation injury missed initially and diagnosed a kind of birth injuries through remarkable periosteal reaction. Conclusion : The birth injuries including clavicle fracture and brachial plexus injury are highly related to high birth weight at birth and the femur fractures are related to delivery maneuver in C-section and breech delivery. Strict observation and monitoring are required because occasionally the paralysis might be the sign of brachial plexus injury or pseudoparalysis due to fracture. By the adequate preterm care or preterm ultrasound check up, high weight delivery could be avoided and then the birth injury must be reduced.

접촉산화수로공법에 의한 수질개선연구

김태철,이규승,오범룡,민진우 忠南大學校 環境問題硏究所 1998 環境硏究 Vol.16 No.-

The effect of non-point source pollution in rural area has much influence on the stream water quality. It is difficult to improve the stream water quality, because of complex pollutant loads technically and because of treatment-cost of the deteriorated water economically. The best way to reduce the non-point source pollution is to check the water quality in the inlet of irrigation channel and control the water quality in the outlet of drainage channel. Stream water quality in the rural area is closely related with the fertilizers, pesticides, and livestock wastewater. Basically the rate of treatment and retention time were estimated for BOD, COD, SS, NH₄-N, T-N, NO₃-N, T-P, PO₄-P using materials such as gravel, crushed stone, useless tire, geotextiles, and concrete block, respectively and also using the combined materials. The rate of treatment for BOD, COD, SS is high, but that for NH₄-N, T-N, NO₃-N, T-P, PO₄-P is low. Optimal retention time in the natural contact channel was 90 minutes.

장시간 수술시 Propofol과 Enflurane의 마취 유지 효과 및 술후 각성도에 대한 비교

김태요,윤재승,이강창,정영표,안선연 圓光大學校 1995 論文集 Vol.30 No.2

There has not been particular attention focused on the comparative benefits and risks of propofol anesthesia with inhalation anesthesia in the operations of long duration. This study was assessed the anesthetic efficacy and the speed of recovery from propofol or enflurane anesthesia in patients undergoing the long term operations. The propofol group (n=25) receiver 2.0 - 2.5mg/kg propofol intravenously for the induction of anesthesia and followed by propofol infusion(6 - 12mg/kg/h). The enflurane group (n=25) was induced as the propofol group and followed by enflurane(1-2 MAC) addministration. All patients received nitrous oxide (50%) in oxygen immediately after tracheal intubation. All anesthetic agents were stopped at the time of last stitch. The hemodynamic changes were recorded and the recovery was assessed with the time from discontinuation of all anesthetics to extubation and the indices of consciousness at early recovery(recall name, eye opening on command, raise hand on request and coughing on request). The recovery tests showed no differences between the groups. Systolic pressure after intubation in propofol group was significantly increased (p<0.05), compared with enflurane group. In propofol group, seven patients received fentanyl because of light anesthetic depth. The frequency of nausea and vomiting was similar between the groups. In conclusion, the long propofol anesthesia was not associated with faster recovery than enflurane anesthesia and propofol as an agent of anesthetic maintenance was unfavorable.

생쥐의 卵子와 初期胚子에 對한 Clomiphene Citrate의 細胞毒性에 關한 硏究

金惠敬,潘勝一,金洋一,文蓮子,朴承澤,鄭然泰 圓光大學校 醫科學硏究所 1993 圓光醫科學 Vol.9 No.1-2

In order to elucidate the cytotoxicity of clomiphene citrate, the rate of in vitro fertilization(IVF) of ova and the developmental rate of early embroys in A-strain mouse were examined. The rate of ova cleavaging to 2-cell stage by IVF was remarkably decreased(12.6%) at the concentration of 10μg/㎖ of clomiphene than that of the control(65.2%) when the ova fertilized in vitro were cultured for 24 hours in the untreated medium after treatment ova with clomiphene for 5 hrs. But there was no ovum cleavaging to 2-cell stage at 50μg/㎖ clomiphene except only a few 2-polar bodied ova. The developmental rate of mouse early embryos was decreased dose-dependently. Especially at 25μg/㎖ of clomiphene, the number of embryos cleavaging to blastocyst from 2-cell stage(41.4%) was decreased remarkably compared with that of the control(87.2%). And only a few embryos were developed to 8-cell stage at clomiphene concentration of 50μg/㎖ after 2-cell embryos were incubated for 72 hours in clomiphene-treated medium. Morphological changes such as fragmentation and fusion of blastomeres, cytolysis and developmental retardation were increased with dose-dependently. These results suggest that clomiphene has cytotoxic effect by the decrease of the rate of IVF, the developmental rate and the degenerative changes of murine ova and early embryos.