A Study on the Functionality of Halophytes from Coast area of Jeollanam-do

Tae-Man Ha,Yang-Joon An,Mi-Yeong Shin,Sook Park,Hak-Jae Park,Hyo-Jeong Kang,Su-Jeong Choi,Jae-Yeong Yu,Ji-Yun Jeon,Gyu-Yeon Oh 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

This study was undertaken to improve the perceived value of halophytes and contribute to the activity of the local economy by analyzing the antioxidant activity and mineral content of Suaeda japonica, Tetragonia tetragonoides, and Spergularia marina that grow wild on the mud flats and coastal areas. The total phenolic compound content found in each order is as follows: Suaeda japonica (3.674∼5.989 mg TAE/g dry wt.), Tetragonia tetragonoides (2.515∼4.471 mg TAE/g dry wt.), and Spergularia marina (2.450∼4.067 mg TAE/g dry wt.); DPPH radical scavenging and ABTS cation radical scavenging activities were also recorded in the same order. In the case of minerals, Iron(Fe) was relatively high in Suaeda japonica (960.1∼1,346.7 mg/kg), Manganese(Mn) in Tetragonia tetragonoides (197.1∼574.8 mg/kg), and Zinc(Zn) was relatively high in Spergularia marina (63.19∼216.56 mg/kg). It was confirmed that the mineral content of Suaeda japonica, Tetragonia tetragonoides, and Spergularia marina was significantly higher than that of Chinese cabbage, lettuce, leek, and spinach.

P3-F계 세제의 살균력 측정

정명주,손태문,박형숙 慶星大學校 1998 論文集 Vol.19 No.2

The microbial activity of P3-luboklar FC,FC-A,FC-A(XT)DX and DXB was compared by DGHM Germ Carrier Test method. The following results were obtained. 1. FC,KB and KC has shown to be effective against all strains within 10 minutes. 2. FC-A was effective against E. coli, immediately, and kill could be achieved against Pseudomonas aeruginosa and Staphylococcus aureus within 4 minutes and 16 minutes, respectively. 3. DXB was effective against all strains within 10 minutes with the exeption of B. subtilis. 4. FC-A(XT) was the most effective against B. subtilis.

원발성 난소 소세포암 2례

조인숙,남계현,김태희,김정식,이해혁,이권해,권계원 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Two cases of primary small cell carcinoma of the ovary occurred. One was a case of 43-year-old woman associated with hypercalcemia and the other was a case of 40-year-old woman with pulmonary type. Ovarian small cell carcinoma of the hypercalcemic type have clinicopathological, immunohistochemical, ultrastructural, and flow cytometric features that from differ from those of small cell carcinomas of the pulmonary type. It is important for therapeutic reasons to distinguish these two types of ovarian tumors from each other and from a variety of malignant small cell tumors that ovary primarily or secondarily. So, we reported these cases with a brief review of the concerned literatures.

Tsukamurella sp. 26A에 의한 생물계면활성제의 생산

최경숙,김순한,정영기,장경립,이태호 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

The strain producing biosurfactant was isolated from soil. The isolated strain was identified as the genus Tsukamurella through its morphological, cultural, physiological, menaquinone type, fatty acid composition characteristics. The highest biosurfactant production by Tsukamurella sp. 26A was observed after 4 days cultivation in the culture medium containing n-hexadecane 7%, NaNO₃0.2%, KH₂PO₄0.001%, K₂HPO₄0.02%, MgSO₄·7H₂O 0.02%, CaCl₂·2H₂O 0.02%, yeast extract 0.02%(pH 6.8-7.0, 30℃). The surface and interfacial tension of an aqueous solution reached 30 mN/m and 1.5 mN/m, respectively. The biosurfactant stabilized oil-in-water emulsion with a variety of hydrocarbones, edible oils and petroleum oils.

골반 내 농양 1예

조인숙,이해혁,김태희,이권해,남계현,이임순,김정식,박성진 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Pelvic inflammatory disease (PID) is a disease that affects young, sexually active, reproductive age women. Most pathogens are Chlamydia trachomatis and Neisseria Gonorrheae transmitted sexually. One of serious acute complication of PID is tubo-ovarian abscess (TOA) that involves the fallopian tube, ovary and adjacent structures (eg, bowel, pelvic peritoneum). The microbiology of TOAs is polymicrobial with anaerobic organisms and causal organisms of PID. Common anaerobic organisms are Peptostretococcus spp, Bacteriodes spp, Prevotella spp, Escherichia coli and aerobic streptococci. Treatment is necessary to admission for intravenous broad-spectrum antibiotics and surgery is often reserved for patients with suspected rupture or patients who fail to respond to antibiotics. So, we reported this case found pelvic abscess due to ruptured tube-ovarian abscess.

항암 치료를 받은 아동의 치아 발육 장애

김민정,이형숙,김신,정태성 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.4

The malignant tumor in childhood is one of the main causes of children s death due to disease. The traditional treatment for the malignancy is known for the radiation therapy and the chemical therapy or both. However, the treatments tend to induce intraoral complications. Different from adults, almost all children on cancer therapy are expected to have dental complications, because their permanent teeth are on the developmental stage. The degree of dental complication depends on the patient's age, type of chemical and other factors-radiation dose and frequency. In this report, 3 children who had experienced the anti-cancer therapy on their age between 1 and 4 years were selected and dental complications were examined. The children have chance for the various oral complications including the developmental problems such as agenesis, microdontia and hypoplasia of the teeth. Therefore, it's important to understand the side-effects of anticancer therapy during the permanent teeth had been developmental stage in young patients. Also, oral health care specialists, including pediatric and hospital dentist can support the oncology team by providing basic oral care, implementing oral care protocols, delivering emergency dental treatment undergoing anticancer treatment. 소아기의 악성 종양은 질병으로 인한 소아 사망의 가장 흔한 원인이다. 이에 대한 대표적인 치료 방법으로 항암 화학 요법과 방사선 요법의 단독 사용 혹은 이들의 병용을 들 수 있다. 그러나 이러한 치료 방법은 다양한 구강 내 합병증을 동반한다. 성인과 달리 소아 환자는 항암치료 시기에 일부 영구치가 활발한 발육단계에 있으므로 발육중인 치아에 치과적 합병증이 예상된다. 치과적 합병증의 정도는 화학약물의 종류, 용량 및 방사선 조사 빈도와 치료 당시 환자의 나이에 따라 달라진다. 본 증례들에서는 특정 영구치의 발육단계에 있는 만 1-4세경에 종양의 치료를 위해 항암 화학 요법과 방사선 치료를 받은 어린이 3명을 대상으로 치료 내용과 치아 발육 상황을 검토해 보았다. 이환된 치아의 수나 그 정도에 차이는 있으나, 각각의 환자에서 영구치 치배의 선천 결손, 왜소치, 치근 저형성 등 발육장애 관련 소견이 관찰되었다. 항암치료를 받은 병력이 있는 소아에 있어서 항암치료 이후에 나타날 수 있는 일반적인 구강 합병증 이외에 발육시기 동안 치아에 미치는 영향을 고려하여, 공간 문제를 포함하여 향후 발생 할 수 있는 다앙한 문제점 예방을 위한 장기적인 관찰과 관리가 필요할 것으로 생각된다.

임신 중 발견된 대망 종괴 1례

조인숙,김태의,김정식,이해혁,이권해,김희경,남계현 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

A case of omental mass during Pregnancy Omental cyst is a rare lesion and occur primarily during childhood and young adulthood. When encountered, most of them are benign and intraabdominal masses. It shows a large mass but not palpable and often asymptomatic. The precise diagnosis is made by most commonly at surgery or during pathologic examination. So, we report this case with brief review of literatures.

부가적 산부식 시간에 따른 자가 산부식 접착제의 법랑질 전단결합강도

이형숙,김성기,이동수,김신,정태성 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.4

이 연구의 목적은 유치와 영구치의 법랑질에 자가 산부식 접착제 적용 전 부가적인 산부식 시간을 달리 하였을 때, 전단결합강도에 미치는 영향을 알아보기 위함이다. 각각 65개의 발거된 건전한 유치와 영구치 협, 설면 법랑질 시편을 제작하고 두 종류의 자가 산부식 접착제를 적용하였다. 실험군은 Clearfil SE bond, Adper Prompt L-pop으로 처리한 두 개 군으로 나누고, 이를 다시 산부식 시간에 따라 여섯 군(0, 5, 10, 15, 20, 30초)으로 분류하였다. 대조군으로는 total-etching system인 Multi-purpose plus를 사용하였다. 접착제 처리면을 복합레진으로 충전하고 24시간 경과 후 전단결합강도를 측정하고 Mann-Whitney test와 Kruskal-Wallis test를 사용하여 통계분석 하였다. 결과는 자가 산부식 접착제를 적용한 군은 유치, 영구치 모두에서 total etching system에 비해 낮은 전단 결합강도를 보였다. 자가 산부식 접착제 사용 군내에서 산부식을 추가로 시행하였을 때 유치와 영구치 모두에서 결합강도가 증가하였으며, 10초 이상 산부식을 시행한 경우 결합력의 차이는 없는 것으로 나타났다. 유치와 영구치간 결합력의 차이는 인정되지 않았다. 결론적으로, 자가 산부식 접착제의 법랑질에 대한 전단결합강도는 유치, 영구치 모두에서 10초 이상의 부가적 산부식이 결합력의 증가에 유리하게 작용하는 것으로 나타났다. The aim of this study was to evaluate the bonding ability of two self etch systems to human primary and permanent enamel and the effect of additional acid etching time. Exfoliated, caries free human primary molar(n=65) and permanent molar extracted (n=65) were used. prepared enamel specimens were randomly divided into 2 test groups and a control group. The control group(n=10) were treated with 35% phosphoric acid gel and Scotchbond Multi-purpose adhesive. Experimental groups(self-etching systems) were subdivided into 6 groups(each n=10) according to additional etching time(0s, 5s, 10s, 15s, 20s, 30s). The result were as follows : 1) The shear bond strengths of the self-etching adhesives(Clearfil SE Bond, Adper Prompt L-pop) without additional etching were lower than control group(Scotch Bond Multipurpose). 2) Between the same self-etch adhesive groups, additional etched groups showed higher shear bond strength 3) There was no significant difference between primary and permanent enamel. In conclusion, bond strength of self-etch adhesives to enamel could be increased with additional etching.

녹차 및 결명자 추출물의 치주조직병 원인균에 대한 억제효과

이은숙,안태영,윤정중,국중기,이병래,김동기 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.4

It is known that the periodontal disease is caused by infection of inner oral bacteria as a suffering disease to human mankind for a long time. It can make the various clinical symptoms such as gingiva, bleeding, formation of periodontal pocket, loss of attached gingiva, collapse of alveolar bone and halitosis, and is known to the major contributor of loss of teeth. This study investigated the growth restraint effect of Camellia sinensis and Casia tora. As a result of study, the minimum restraint concentration of standard microbe on F. nucleatum for extracts from C. sinensis showed 2.5, 0.63 and 1.25 mg/ml, respectively and minimum restraint concentration of clinical separation microbe on F. nucleatum for extracts from C. sinensis showed 2.5 and 5.0 mg/ml. The minimum restraint concentration of standard microbe and clinical separation microbe, respectively. Meanwhile, the minimum restraint concentration of standard microbe and clinical separation microbe on F. nucleatum for extracts from C. tora showed 2.5 and 2.5-5.0 mg/ml, respectively. The minimum restraint concentration of standard microbe and clinical separation microbe for P. intermedia, P. nigrescens, P. gingivalis, and P. endodontalis showed above 5.0 mg/ml for standard microbe and above 5.0 mg/ml for clinical separation microbe, respectively. As a result of investigating the cell toxicity for each extracts, extracts from C. sinensis does not showed the toxicity for the cell below the concentration of 2.5 mg/ml, but it showed toxicity for the cell above concentration of 2.5 mg/ml and it decreased the existence ratio for the cell. Meanwhile, in the case of C. tora, it increased the multiplication ratio below the concentration of 1.25 mg/ml compared to the subject group and it decreased gradually the multiplication ratio that the growth ratio for the cell is increased better than subject group. As a result of this study, extracts from C. sinensis was more effect on the growth restraint effect against periodontopathogens than extracts from C. tora and is proven that it is possible for use as a oral mouthrinsing liquids in the near future. On the basis of results of this study, it is judged that the study for reaction mechanism and discrete clinical test for implementation should be needed.

제대혈 분리방법의 차이에 따른 단핵구 및 조혈모세포의 수득 효과에 관한 비교연구

박정숙,이영호,최안홍,노신애,김태겸,한진영 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.1

연구배경 : 최근에 보고된 변형적 Ficoll-Hypaque 방법을 이용한 제대혈 분리방법이 지금까지의 고식적 Ficoll-Hypaque 분리방법에 비하여 조혈모세포의 수득효과면에서 얼마나 차이가 있는지 또한 비교적 수득효과가 크다고 알려져 있는 3% gelatin을 이용한 분리방법에 비하여 얼마나 효과적인지를 알아보고, 제대혈 분리방법의 차이에 따라 단핵구와 조혈모세포 사이에 어떤 상관관계가 있는지 알아보기 위하여 본 연구를 시행하였다. 방법: 21명의 건강한 산모로부터 정상 질식 및 제왕절개 분만 후 제대정맥에서 해파린 처리한 주사기를 사용하여 제대혈을 채취하였다 채취한 제대혈은 동일하게 3개월 시험관에 나누어 실온에서 24시간 이내에 고식적 Ficoll-Hypaque분리법, modified Ficoll-Hypaque 분리법, 3% gelatin 분리법을 이용하여 각각 단핵구 분리를 시도하였으며 이들의 단핵구수 및 생존률, CD34 양성세포수, 집락수를 측정 비교 하였다. 또 상관분석을 이용하여 단핵구수, CD34양성세포수와 CFU-GM집락수 사이의 상관관계를 알아보았다. 결과: 1) 세포 생존률은 고식적 Ficoll-Hypaque 분리법, 변형적 Ficoll-Hypaque 분리법, 3% gelatin 분리법 사이레 통계적 차이가 없었다. 2) 단핵구수는 3% gelatin으로 분리했을 때가 4.56±1.86 (×10^(6)/mL)로 수득효과가 가장 높았으며 (P=0.0001), Ficoll-Hypaque으로 분리했을때는 각각 1.54±0.06(×10^(6)/mL), 1.36±0.67(×10^(6)/mL)로서 고식적 방법과 변형적 방법 차이에 통계적 차이가 없었다. 3) CD34 양성세포수는 3%gelatin으로 분리했을 때가 6.08±3.99(×10⁴/mL)로 수득효과가 가장 높았으며 (P=0.0001), Ficoll-Hypaque으로 분리했을 때는 각각 2.18±2.17(×10⁴/mL), 2.24±1.63(×10⁴mL)로서 고식적 방법과 변형적 방법사이에 통계적 차이가 없었다. 4) CFU-GM 집락수는 3% gelatin으로 분리했을 때가 15.72±8.93(×10³/mL)로 수득효과가 가장 높았으며 (P=0.0002), Ficoll-Hypaque으로 분리했을 때는 각각 3.12±1.45(×10³mL), 2.35±0.82(×10³/mL)로서 고식적 방법과 변형적 방법 사이에 통계적 차이가 없었다, 5) 제대혈 고식적 Ficoll-Hypaque 방법으로 분리하였던 경우는 단핵구수와 CFU-GM집락수 사이에 상관관계가 없었으며CD34 양성세포수만 CFU-GM집락수와 상관관계가 있었다. (r=0.57). 또 변형적 Ficoll-Hypaque 방법에 의하여 분리하였던 경우는 단핵구수나 CD34 양성세포수 모두가 CFU-GM 집락수와 상관관계가 없었다. 그러나 제대혈을 3% gelatin법으로 분리하였던 경우는 CFU-GM집락수 가 단핵구수 (r=0.88) 및 CD34양성세포수(r=0.86)양자 모두와 상관관계가 있었다. 결론 : 3%gelatin을 이용하여 제대혈을 분리하는 것이 단핵구 및 조세모세포의 수득효과면에서 가장 효과적이었다. 한편 변형적 Ficoll-Hypaque 분리법이 고식적 Ficoll-Hypaque 분리법보다 시간과 경비면에서 비효율적이면서 수득효과도 높지 못하였다. 또 제대혈 분리법의 차이에 따라 단핵구나 CD34 양성세포와 VFU-GM사이에 상관관계에 차이가 있었으며, 실제로 제대혈이식 후에 생착 여부를 예측할 수 있는 인자가 제대혈 분리법의 차이에 따라 달라지는지에 관해서 보다 많은 임상적 연구가 필요할 것으로 사료된다. Background : Cord blood banking for stem cell transplantation requires volume reduction and red cell depletion for cost effective storage. To determine which technique could minimize the loss of progenitor cells during cord blood processing, we compared the efficacy of cord blood separating methods which were conventional Ficoll-Hypaque method, modified Ficoll-Hypaque method, and 3% gelatin method. Methods : Twenty-one cord blood samples were obtained with heparinized syringes from the umbilical vein following delivery. Three aliquots of each 20mL cord blood were stored at room temperature and processed by 3 different techniques within 24 hours after collection. We compared mononuclear cells(MNCs) counts and their viability, CD34+ cells counts, and CFU-GMs counts and also observed their correlations among three techniques. Results : 1) The yield of MNCs or CD34+ cells was highest in the cord blood processed with 3% gelatin method than conventional or modified Ficoll-Hypaque method(P=0.0001). 2) There were no significant difference in the yield of MNCs and CD34+ cells between conventional and modified Ficoll-Hypaque method. 3) Both MNCs counts (r=0.800) and CD34+ cells counts (r=0.86) were significantly correlated with CFU-GM counts in the cord blood processed with 3% gelatin method. 4) CD34+ cells counts were significantly correlated with CFU-GM counts in the cord blood processed with conventional Ficoll-Hypaque method (r=0.57), but MNC counts were not. 5) Both MNC counts and CD34+ cell counts were not correlated with CFU-GM counts in the cord blood processed with modified Ficoll-Hypaque method. Conclusion : Cord blood separation with 3% gelatin method could provide better yield of progenitor cells than with FICOLL_Hypaque methods. And between two Ficoll-Hypaque methods, modified method is more laborsome but not better in terms of clonogenic potential than conventional method.