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Bis-Diazotized Benzidine에 의한 연속 및 동시흡착법에 의하여 Tuberculin PPD로 감작된 적혈구를 이용한 혈구응집반응의 민감성
김택선,정상인,최철순,양용태 중앙대학교 의과대학 의과학연구소 1984 中央醫大誌 Vol.9 No.3
Passive haemagglutination test(PHA) using sheep red blood cells(SRBC) sensitized with soluble antigens by means of coupling agents, i.e., tannic acid, bis-diazotized benzidine(BDB), chromic chloride, glutaraldehyde, and so on, provide an extremely sensitive technique to detect a minute quantity of serum antibody as low as 0.01㎍. It also has advantage over agglutination and precipitation tests in that if it is transferred either to hemolysis test(PHL) or to inhibition test(PHI), soluble antigen-antibody complexes and incomplete or hapten antigen-nonprecipitating antibody complexes that are not detectable by other tests can be demonstrated. This study was undertaken to determine the optimum amount of BDB and tuberculin PPD antigen for consecutive and simultaneous coupling of SRBC in conjunction with the sensitivity of the PHA test. In addition, the sensitivities of PHA and PHL test were compared and the PHA units of antibody and diluents for tuberculin PPD antigen for PHI test were discussed. The results are summarized as follows: 1. The optimum amount of BDB for consecutive and simultaneous coupling of 0.2ml of 2.5% SRBC were 20㎍ and 13㎍, respectively, and the optimum amount of tuberculin PPD antigen were about 125㎍ and 250㎍ respectively. The optimum ratios between BDB and tuberculin PPD antigen for consecutive and simultaneous coupling were 1:6.3 and 1:19.2, respectively. 2. In the consecutive coupling, the PHA titre was linear to both BDB and tuberculin PPD concentrations, whereas in simultaneous coupling it was affected by the level of tuberculin PPD but not by that of BDB from 5㎍ to 13㎍. 3. Using SRBC sensitized consecutively with tuberculin PPD or sensitins prepared from four species of mycobacteria the homologous PHL tests were sensitive than the PHA tests by 1:2 to 1:4 dilutions, and the end-point of PHL test was more clear-cut than that of PHA test.
韓久雄,鄭鍾學,南澤昇 순천향대학교 1984 논문집 Vol.7 No.1
The mass health screening examination was excuted differently from the primary screening test and secondary precision test for 2,718 teachers in Gumi City and its adjoined three Guns, Kyungpook Province, 1982. The age of subjects was between 20 and 64 years old, and the percentage of men teachers showed 74 and the percentage of women teachers was 26. The results of the screening tests are summarized as follows: Of 2,718 subjects, 16.5% (6.8% for hypertension, 4.2% for liver disease, 2.8% for pulmonary tuberculosis and 1.8% for diabetes) required secondary precision test. Detected cases account for 63.8% of the 447 teachers taking secondary precision test, this was equivalant to 10.5% of the total examined teachers. 3.3% of the total examined teachers turned out to be confirmed cases and 7.2% of the total examined teachers was suspicious cases. The confirmed cases of the total examined teachers by disease were 1.0% for prevalence of pulmonary tuberculosis, 1.4% for prevalence of hypertension and 0.8% for prevalence of diabetes. The pervalence rate obtained by age group were 26.1% for 50~59 age group which was the highest rate group, 16.0% for 40~49 age group and 6.6% for 30~39 age group. It was shown that the rate increased by age. Although hypertension, diabetes and pulmonary tuberculosis increased by being old, in cases of liver disease, renal disease and anemia, 30~49 age group was marked as high rate.
Lipase - supported Preparation of Optically Active 2- Amino Alcohols of Biological Interest
Chung, Sung Taik,Kim, Sang Han,Jin, Myung Jong 한국공업화학회 1997 Journal of Industrial and Engineering Chemistry Vol.3 No.3
Transesterification of racemic N-acetylated 2-amino alcohols with vinyl acetate was investigated in the presence of inexpensive commercial porcine pancreatic lipase. The enzymatic kinetic resolution occurred with high reactivity and enantioselectivity to give optically pure N,O-diacetylate and N-monoacetylate. This result clearly indicates that the biocatalytic route can be efficiently used in the preparation of enantiopure 2-amino alcohol.
A Simple Method to Determine the Adsorption Isotherm of Taxol from a Yew Tree
Chung, Sung Taik,Row, Kyung Ho,Park, Young Kwang 한국화학공학회 1999 Korean Journal of Chemical Engineering Vol.16 No.3
Taxol, a powerful anticancer agent, was extracted from yew extract and separated by NP-HPLC (Normal-Phase High Performance Liquid Chromatography). At the mobile phase composition of hexane/IPA/MeOH, 90/4/6 vol%, taxol was preparatively separated in a chromatographic column with large packings (15 ㎛). The purified taxol was used to determine the adsorption isotherm of taxol. From the experimental results, the adsorption isotherm was obtained as the following Langmuir type, To obtain the adsorption isotherm, only two injections of a small and large amount of taxol were required, and the Langmuir adsorption parameters were calculated from the assumption of monolayer coverage on the solid surface.
SEPARATION OF L-VALINE BY ANIONIC CARRIER - MEDIATED TRANSPORT IN A SUPPORTED LIQUID MEMBRANE
Chung, Sung Taik,Yi, Min Hoe,Nam, Se Jong 한국화학공학회 1997 Korean Journal of Chemical Engineering Vol.14 No.4
An anionic carrier-mediated separation of L-valine from the dilute aqueous solution was studied with a liquid membrane, constituted by a solution of D2EHPA (Di-2-Ethyl Hexyl Phosphoric Acid) in 1-decanol and supported by a hydrophobic microporous membrane. The equilibrium and transport rate data are reported in terms of distribution coefficients and mass transfer coefficients under the varying conditions of feed phase pH and carrier concentration. The experimental results obtained are analyzed to confirm the mechanism of the complex formation between carrier and L-valine and to describe the transport rate of L-valine through the supported liquid membrane by an appropriate theoretical model. The selectivity and the stability of the supported liquid membrane under study were also evaluated to ensure its potential application as a commercial down-stream process.
New Chiral Catalyst for Asymmetric Alkylation of Aldehydes with Diethylzinc
Chung, Sung Taik,Kim, Sang Han,Ahn, Sum Jin,Jin, Myung Jong 한국공업화학회 1997 Journal of Industrial and Engineering Chemistry Vol.3 No.1
Asymmetric alkylation of aromatic aldehydes with diethylzinc in the presence of catalytic amount of chiral thiazolidines derived from L-cysteine ethyl ester afforded the corresponding optically active secondary alcohols.
Enantioselective Addition of Diethylzinc to Aldehydes Catalyzed by Chiral Hydroxymethylthiazolidines
Chung, Sung Taik,Kim, Sang Han,Jin, Myung Jong 한국공업화학회 1998 Journal of Industrial and Engineering Chemistry Vol.4 No.4
A series of chiral thiazolidine-based β-amino alcohols 2a-c was readily synthesized from enantiomerically pure L-cysteine ethyl ester hydrochloride. These compounds could be used as chiral catalysts in enantioselective addition of diethylzinc to aldehydes. Particularly, 2c was found to be the most efficient catalyst, which produced optically active secondary alcohols with moderate enantioselectivity (up to 76% ee) in good yield.
Enantioselective Addition of Diethylzinc to Aldehydes Catalyzed by MCM - 41 - Supported Ephedrine
Chung, Sung Taik,Kim, Sang Han,Jin, Myung Jong,Ong, Ho Joon 한국공업화학회 2001 Journal of Industrial and Engineering Chemistry Vol.7 No.4
MCM-41 silica-supported ephedrine was prepared and utilized as an asymmetric catalyst for enantioselective addition of diethylzinc reagent to aldehyde. The MCM-supported ephedrine led to much faster reaction and higher asymmetric induction than silica gel-supported ephedrine. Highly ordered MCM-41 silica was found to be better inorganic support for the catalytic system than amorphous silica gel.