Tryophagus putrescentiae induces inflammatory cytokine secretions from the human cell lines THP-1 and EoL-1 cells

Jeon Sang‐Jin,Kim Eun Joo,Oh Jihyun,Yun Chi‐Young 한국곤충학회 2020 Entomological Research Vol.50 No.7

The present study aimed to identify the roles of the storage mite Tyrophagus putrescentiae (Tp) in relation to human skin diseases, such as atopic dermatitis. We examined the effects of T. putrescentiae extracts (TpE) on the secretion of inflammatory cytokines and their mRNA expression in human immune cells. Human monocytic THP-1 and eosinophilic EoL-1 cells were challenged with TpE and the inflammatory cytokines, interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) secreted in culture supernatant were analyzed using an enzyme-linked immunosorbent assay (ELISA). The mRNA levels in the cell cultures were also measured via reverse transcriptase-polymerase chain reaction (RT-PCR). In the THP-1 cells, the TpE significantly induced the secretion of IL-6, IL-8, and MCP-1 in a time- and dose-dependent manner. It also stimulated increases in the expression levels of the IL-6, IL-8, and MCP-1 mRNA. However, while the TpE induced IL-8 and MCP-1 mRNA levels in the EoL-1 cells, it did not induce IL-6 expression. Using Western blot analysis, we confirmed the activation of extracellular signal-regulated kinase (ERK), a member of the mitogen-activated protein kinases (MAPKs), in response to TpE challenge of the THP-1 cells. Collectively, our results show that TpE challenge elicits inflammatory response in the human monocytic THP-1 and eosinophilic EoL-1 cells, and it induces increased production of the inflammatory cytokines IL-6, IL-8, and MCP-1.

한국인의 우울증 진단기준의 특성

장성만,손지훈,이준영,최지환,조성진,전홍진,함봉진,이동현,조맹제 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

목적 국내의 여러 역학 조사에 따르면 한국인은 서구에 비하여 우울증의 유병률이 낮은 것으로 보고되고 있다. 낮은 유병률에 대하여 서구에서 정의하는 우울증의 진단 기준이 한국인의 문화에 맞지 않다는 진단 기준의 오류를 제시하거나, 실제로 유병률이 낮다는 설명을 시도하기도 하였다. 본 연구는 DSM-IV에서 제시하는 우울증의 진단 기준에 대하여 한국인의 반응 특성을 알아보고자 한다. 방법 2001년 KECA 연구에 응답한 만 18세 이상 65세 미만의 6,275명을 대상으로 하였다. 한국어판 CIDI (K-CIDI)를 통해 정신장애의 이환을 진단하였으며, K-CIDI 내의 우울장애 진단 기준만을 추출하여 분석하였다. 분석은 문항반응이론(Item Response Theory)을 적용하였다. 결과 한국인에서 DSM-IV의 주요우울장애 진단 기준의 오류는 없었으나, 진단 기준의 역치는 높았다. 한국인이 호소하는 우울증상으로서 "피로감"과 "집중력 저하", "수면 변화"는 진단되는 우울증의 초기부터 잘 나타나는 증상 들이며, "정선운동변화", "죽음/자살사고", "무가치함/죄책감"은 보다 심한 우울증에서 잘 나타나는 증상들이었다. 결론 DSM-IV가 제시하는 주요우울장애 진단 기준은 한국인의 우울증을 평가하는 데 있어 타당하기는 하나, 진단 기준의 역치가 높다. 우울증의 중증도에 따라 호소하는 증상들이 달랐다. Baekgrounds : Many Korean epidemiologic studies reported lower prevalence rates of depression than those of Western countries. For the low prevalence, it is explained that there's something wrong in the direct application of diagnostic criteria of depression to Korean culture, i.e. categorical fallacy, or it may be truly low-prevalent. We will analyze diagnostic criteria for depression detined by Western. Methods : Six thousand and two hundred seventy-five cummunity dwelling subjects, aged 18-64 years were interviewed by using Korean version of Composite International Diagnostic Interview (K-CIDI). Diagnostic criteria for depression defined by DSM-IV were analyzed using the item response theory. Rcsults : We could not find any fallacies of diagnostic critcria tbr depression defined by DSM-IV when assessing depre-ssion among Koreans. Fatigue, concentration difficultics, and sleep disturbance appeared more fequently in mild depression, while psychomotor change, death/suicide, and worthlessness/guilt did not appear until severe depression. Cunclusion : The diagnostic criteria for depression defined by DSM-IV arc appropriate for the Koreans. There are different responding levels, i.e. threshold, of depressive criteria according to severities of depression. Koreans with depression are more likely to complain of appetite change, but less of worthless or guilty feelings than Western people.

정신보건관련시설 입원(소)자의 주요 정신장애 유병률 : 전국 20개 시설의 입원(소)자를 대상으로

조성진,조맹제,서동우,함봉진,홍진표,배재남,이준영,이동우,박종익,전홍진,김성주,김용익 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.6

Objectives : One of the objectives of this study is to estimate the prevalence rates of psychiatric diagnoses in the combined populations of psychiatric hospitals, psychiatric nursing facilities and homeless asylums using the Korean version of the Composite International Diagnostic Interview (K-CIDI), which is a comprehensive and fully standardized interview schedule to assess psychiatric disorders for diagnosis. The Other objective is to compare with previously studied prevalence rates of Psy-chiatric diagnoses using the results of this study. Methods : The study subjects, aged from 181o 64 years, were randomly selected from 64,582 institutionalized population of psychiatric hospitals, Psychiatric nursing facilities and homeless asylums as of 30, June, 2001. Twelve trained interviewers administered the K-CIDI to the selected respondents. A total of 1,875 respondents (male 1,194, female 681) completed the interview. Results : The lifetime and one year prevalences of any diagnosis excluding nicotine dependence · withdrawal, anxiety disorder, eating disorder, somatoform disorder were 88.2% (male 88.1%, female 88.3%), and 65.8% (male 62.7%, female 73.7%), respectively. The lifetime and one year prevalences of schizophrenia and other psychotic disorders were 78.5% (male 75.3%, female 83.7%), and 56.8% (male 51.9%, female 64.8%), respectively. The lifetime and one year prevalences of alcohol use disorder (dependence/abuse) were 26.7% (male 37.9%, female 8.3%), and 8.7% (male 12.8%, female 2.1%), respectively. The lifetime and one year prevalences of mood disorder were 18.1% (male 13.5%, female 25.8%), and 10.4% (male 7.2%,female 16.9%), respectively. The lifetime and one year prevalences of schizophrenia and other psychotic disorders were comparable with the 2001 community survey by 1.19% to 1.32%, and 0.51% to 0.61%, respectively. The lifetime and one year prevalences of schizophrenia were also comparable by 0.16% to 0.28%, and 0.16% to 0.25%, respectively. There were no significant changes of prevalence rates when correction were applied to other psychiatric diagnosis. Conclusion : The results of this study could be used for evaluating the distribution of psychiatric diagnoses in mental health related facilities and for planning mental health policies.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

일차성 Streptococcus pneumoniae 균혈증에 동반된 흉부 하행 대동맥염 1예

전민혁,고옥배,정우진,최영준,이정훈,최상호,김미나,김양수,우준희,류지소 대한감염학회 2005 감염과 화학요법 Vol.37 No.5

페렴구균에 의한 대동맥염은 국내에서 1예만 보고될 정도로 매우 드문 감염증이다. 저자들은 원발성의 폐렴구균혈증에 합병된 페렴구균 대동맥염 환자 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Bacterial aortitis is rare and without surgical intervention, it usually leads to uncontrolled sepsis or catastrophic hemorrhage, ultimately, resulting in death. In the postantibiotic era, the dominant infecting organisms are Staphylococcus aureus and Salmonella spp.. In spite of frequent pneumococcal bacteremia, vascular seeding is rare and there is only one published report in Korea. We describe a 77-year-old female with primary pneumococcal bacteremia followed by aortitis of the descending aorta, which was successfully treated by aneurysmectomy, wide excision of periaortic tissue, aortobiiliac bypass via prosthetic graft, and antibiotic therapy.

외경 0.25mm 의 동맥문합에 있어서 항혈전제의 효과

전대우,강진성,한기환 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

In 1960, Jacobson and Suarez reported a 100 per cent patency rate following the anastomoses of vessels which were 1.6 to 3.2 mm in diameter. This report speeded modern-day microvascular surgery up for the last 20 years. High patency rate is reported in microvascular anastomosis of the 1.0mm diameter, and microvascular surgery is widely used in clinical practice. Improvement in methosds of microanastomoses and development of the microvascular instruments, including the operating microscope along with the microsutures make the microvascular anastomoses of vessels that are less than 0.5mm in diameter opssible. The key problem microvascular surgery is the formation of thrombus which is mainly composed of platelets, causing occlusion of the anastomotic site. So much efforts have been made in developing effective anticoagulants. Currently used systemic anticoagulants are heparin, aspirin and persantin. Aspirin inhibits collagen-induced platelet aggregation and the primary action of persanin is to inhibit platelet aggregation to ADP, collagen, and epinephrine, is widely used as effective anticoagulant for patients of atherosclerosis, hemodialysis, and subarachnoid hemorrhage. An animal study was carried out to investigate the opssibility of tichlopidine-HCI to be used as a choice of anticoagulant in microvascular surgery. One hundred and twenty rats were divided into 3 groups. Group A was the control group. Aspirin and Persantin were given orally in group B. Ticlopidine-HCI was given orally in group C. A superficial epigastric artery as small as 0.25mm in hage devised autogenous fascia and silicone rubber tube method to splint the suture line and reduce the bleeding casued by anticoagulants. Fewer sutures were required, saving time and effort in microvascular repair. The results were as follows: 1. At 20 minutes after anastomoses, the patency rates were 100% in all groups. 2. At 3 days after anastomoses, the patency rates were 75% in the control group(A), 75% in the aspirin-persanting group(B), and 80% in the ticlopidine group(C). 3. At 3 weeks after anastomoses, the patency rates were 70% in the group A, 75% in the group B, and 80% in the group C. It is concluded that the ticlopidine-HCI may be used in the microvascular anastomoses of very small arteries as the anticoagulant of choice.

항공기 개념설계를 위한 천음속 영역의 공력특성 예측에 관한 연구

전권수,김상진,이재우,변영환 건국대학교 산업기술연구원 2000 건국기술연구논문지 Vol.25 No.-

During the aircraft conceptual design phase, rapid aerodynamic analyses over various configurations are required. Hence, empirical and analytic techniques play important roles in estimating the aerodynamic characteristics. In this study, the aerodynamic characteristic estimation technique for transonic region based on empirical and analytical is developed. By comparing with the results of the wind tunnel experiment and existing prediction method, it is verified that the present method is accurate and useful.

정신분열병 환자에서 세가지 청각 자극 "Oddball" 모형에 의한 사건관련전위 P3a와 P3b

진용탁,박이진,남지민,한상익,전양환 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.5

Objectives : Using 3-stimulus auditory "oddball" paradigm reflecting fronto/central and temporo/parietal functions Simultaneously, we examined patients with schizophrenia. Methods : fifteen patients with schizophrenia from outpatient clinic and fifteen normal controls from hospital staffs were recruited for the study. To elicit P3a and P3b, 3-stimulus auditory oddball paradigm was employed. The 3-stimulus auditory oddball paradigm was composed of standard tone (1,000 Hz, 75 dB, 80%), target tone (2,000 Hz, 75 dB, 10%) and distracter (White noise, 95 dB, 10%). Results : P3a and P3b were prominent in fronto/central and temporo/parietal areas, respectively, in both schizophrenics and normal controls. The P300 amplitude in patients with schizophrenia was reduced across P3a and P3b (p<0.01). The P300latency in patients with schizophrenia was delayed across P3a and P3b (p<0.01). Conclusion : These results were consistent with frontal and temporo-parietal lobe dysfunctions in schizophrenics. The 3-stimulus auditory paradigm could be applied for patients with schizophrenia and useful for further exploration the disorder.

Retinol 국소도포에 의한 눈가주름 제거효과

전희대,이성열,이혜진,박영립,이종석,황규왕 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

In 3 months randomized, double-blind, vehicle-controlled study of retinol (vitamin A 0.1%-3000IU/g) in the treatment of photoaging skin, especially eye wrinkles. 24 patients applied retinol contained cream to the both eye wrinkles and vehicle cream(placebo) to the same site. Half of the patients received retinol contained cream and half received vehicle cream(placebo). All 24 patients who completed the study showed statistically significant improvement in photoaging eye wrinkles on the retinol contained cream treated group, but not on the vehicle treated group. Eight of the 12 patients who received retinol contained cream to eye wrinkles had improvement in photoaging, whereas only three of the 12 patients who received vehicle cream(placebo) improved. In retinol contained cream group, there is a some relationship between water contents and effects. In other words, as water contents were the higher, effects were the better. The side effects were limited to mild irritation of retinol contained cream treated skin. Retinol contained cream appeared to be safe and effective in the treatment of eye wrinkles.

회맹장 및 대장을 침범한 장 결핵에 있어서 바륨을 이용한 방사선 이중조영촬영 소견에 대한 연구

진용현,전혜정,이용근,강철호,조성범,김윤환,박철민,차상훈,김일영,이혜경 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

To determine the characteristic double contrast barium enema findings of ileo-cecal and colonic tuberculosis and to make differential diagnosis from other bowel inflammatory disease. Sixty-seven patients confirmed as ileo-cecal and/or colonic-tuberculosis in six hospitals were evaluated retrospectively. The diagnosis was proven by endoscopic biopsy, surgery, and clinical follow up. Nine of 67 patients were excluded because radiologic findings were normal though endocopic biopsy examinations resulted in tuberculosis. Histopathological diagnosis were madein 58 cases as follows: endoscopic biopsy(n=29), operation(n=12), follow up examination(n=14), tissue culture(n=1), laparoscopic biopsy(n=2). There were 30 men and 28 females. The mean age were 36 yrs(range:16-67), 34 patients were in the second and third decade. The distribution of lesions, morphological characteristics, mucosal changes, ulcer shape and depth, associated with other tuberculous lesion, multiplicity were retrospectively analyzed in the findngs of double contrast barim enema. The commonest clinical symptoms were abdominal pain(n=35, 60%) and diarrhea(n=18, 31%). The chest radiography revealed association of pulmonary tuberculosis in 39 cases. Tuberculous lesions of other organs except chest were present in five cases. Double contrast barium enema findings showed a thickening and irregularity of intestinal mucosal fold(n=29, 50%), bowel contraction and/or shortening(n=26, 45%). There were small, nodular, superficial ulcers in the case of ulcer depth less than 2 mm in the depth. As many as 39 cases(67%) in this series had two or more lesions in the intestinal tuberculosis. The involved segment revealed symmetrical feature(n=21, 36%). Inflammatory pseudopolypoid lesions were noted in 30 cases(52%). The ileo-cecal region was involved in 38 cases(66%). The ascending colon(n=25, 43%) was the second commonest site with or without contiguous cecal involvement. Lesions in terminal ileum, ileo-cecal valve, transverse colon, descending colon and appendix were seen in 19, 18, 18, 12 and seven cases, respectively. Among 18 cases of ileo-cecal valve lesions, there were incompetency in nine cases(16%) and wide gapping in four cases(7%). In 14 cases, tuberculous enterocolitis was diagnosed by clinical follow-up and double contrast barium enema study. 12 out of 14 cases, the findings of double contrast barium enema of tuberculous enterocolitis were normalized after anti-tuberculous medication. However, two cases showed more aggravated state than before due to inappropriate treatment. Double contrast barium enema plays an important role in the diagnosis of tuberculous enterocolitis, determines the type, site and extent of the involvement and helps in treatment guide by using sequential studies.