Humic acid가 카드뮴 어독성에 미치는 영향

류지성,정규혁,최필선,이길철,최덕일,최성수,류홍일,박광식 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Cadmium, a major aquatic pollutant in many parts of the world, is toxic and readily accumulated in aquatic organisms. It mainly exists in water as complexes with organic ligands such as dissolved organic carbon and this complexation is known to have effects on the aquatic toxicities. In this study, acute toxicity, histology, and bioaccumulation were studied to evaluate effects of humic acid on cadmium toxicity and bioavailability to fish using Oryzias latipes and Cyprinus carpio. 96h-LC50 of cadmium was 6.38 ㎎/L using Oryzias latipes. However, the mortality showed the dose-dependent decrease when humic acid was added to the test solution. When fish were exposed to 5㎎/L of cadmium, histological changes in the exposed organs(caudal fins, gills, kidney, liver, and gut) of Cyprinus carpio were decreased by humic acid, especially in kidney and liver. Bioaccumulation of cadmium also decreased by treatment of humic acid. It seems that the formation of complexes between cadmium and humic acid may decrease bioavailability of cadmium to fish, and thus reduce the toxic effects of cadmium.

Humic acid가 카드뮴 어독성에 미치는 영향

류지성,정규혁,최필선,이길철,최덕일,최성수,류홍일,박광식 한국환경독성학회 1998 환경독성보건학회지 Vol.13 No.1

Cadmium, a major aquatic pollutant in many parts of the world, is toxic and readily accumulated in aquatic organisms. It mainly exists in water as complexes with organic ligands such as dissolved organic carbon and this complexation is known to have effects on the aquatic toxicities. In this study, acute toxicity, histology, and bioaccumulation were studied to evaluate effects of humic acid on cadmium toxicity and bioavailability to fish using Oryzias latipcs and Cypriruts carpio. 96h~LC50 of cadmium was 6.38 mg/L using Oryzias latipes. However, the mortality showed the dose-dependent decrease when humic acid was added to the test solution. When fish were exposed to 5 mg/L of cadmium, histological changes in the exposed organs (caudal fins, gills, kidney, liver, and gut) of Cyprinifs carpio were decreased by humic acid, especially in kidney and liver. Bioaccumulation of cadmium also decreased by treatment of humic acid. It seems that the formation of complexes between cadmium and humic acid may decrease bioavailability of cadmium to fish, and thus reduce the toxic effects of cadmium.

한약수치에 관한 연구(제 9보) : 炒甘草 제법의 표준화 및 규격화(1) Preparing Standardization and Regulation of Stir-Frying Glycyrrhzia root(1)

최혁재,이우정,박성환,송보완,김동현,김남재 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

In this study, We carried out the preparing standardization and regulation of processed Glycyrrhizae Radix(PGR) which have been widely used in oriental medicines. Glycyrrhizae Radix(GR) have been generally prepared by the stir-frying, or mix-frying with honey for purpose of decreasing sweetness and augmenting vitality. Firstly, we tried to standardize PGR prepared by the stir-frying. We purchased 14 kinds of PGR and non-processed GR(NPGR) at oriental physician's offices and oriental pharmacies on a nation scale, respectively. The amounts of dry on loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, glycyrrhizin(GL), glycyrrhetic acid(GA) and liquiritin(LQ) of them were examined. The amounts of dray on loss, GL and LQ in commercial PGRs showed remarkable decrease, while GA showed increase as compared with NPGR. In order to standardize preparing method of PGR, the effect of heating time on physico-chemical parameters and biological activities were examined. Physico-chemical parameters such as dry on loss, extract amount, GL and LQ contents in PGRs showed decrease, however, GA was increased with heating time as compared with NPGR. Also, GA obtained form heat-treated GR, was found as an artifact in PGRs. PGR was more effective than NPGR in vitro test of DPPH scavenging effect and TBA-Rs reducing effect. PGR and NPGR showed potent hepatoprotective effect on CCl_(1)-intoxicated rats. Especially, PGR prepared by 80 min of heating was the most effective. Considering these results, the optimal condition for PGR preparation was 150℃ for 80min.

레진 시멘트의 film thickness가 간접 복합 레진 수복물의 접착 효율에 미치는 영향에 관한 연구

이상혁,최기운,최경규 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.2

본 연구는 여러 레진 시멘트의 film thickness에 따른 상아질과 간접 레진 수복물 간의 미세인장 결합 강도를 측정하고, 각 레진 시멘트의 중합 수축 및 굴곡 강도, 탄성 계수를 비교 분석하며, 접착 계면 및 파단면의 주사전자 현미경 관찰을 통하여 레진 시멘트의 film thickness가 레진 간접 수복물의 접착 효율에 미치는 영향을 평가하고자 시행하였다. 복합 레진형 레진 시멘트인 Variolink II와 Duo-Link, 접착형 레진 시멘트인 Panavia F와 Rely X Unicem의 4가지 레진 시멘트를 사용하였고, 각각의 시멘트를 film thickness에 따라 < 50 μm (control)의 대조군과 50 μm (T50), 100 μm (T100), 150 μm (T150)의 실험군으로 나누어 총 16개의 군으로 분류하였다. 데이터는 ANOVA와 Duncan’s multiple comparison test (p < 0.05)를 이용하여 통계 분석하였으며 다음과 같은 결론을 얻었다; 1. Variolink Ⅱ는 모든 film thickness에서 접착형 레진 시멘트보다 높은 결합 강도를 나타냈지만(p < 0.05), Duo-Link는 대조군을 제외하고는 통계학적 유의차가 없었다(p > 0.05). 2. Film thickness가 증가할수록 복합 레진형 레진 시멘트의 결합 강도는 유의성 있게 감소(p < 0.05)하는 경향을 보인 반면, 접착형 레진 시멘트는 통계학적 유의차가 없었다(p > 0.05). 3. Panavia F는 통계학적으로 유의성 있게 낮은 중합 수축량을 나타냈다(p < 0.05). 4. 굴곡 강도와 탄성 계수는 복합 레진형 레진 시멘트가 접착형 레진 시멘트보다 유의성 있게 높게 나타났다(p < 0.05). 5. FE-SEM 관찰 결과 결합 강도가 높은 복합 레진형 레진 시멘트는 균일한 접착층과 잘 발달된 resin tag 소견을 보였으나, 접착형 레진 시멘트는 불분명한 접착층과 resin tag 소견을 나타냈다. 파단면 관찰에서 복합 레진형 레진 시멘트는 혼합형 파괴 양상을 나타냈으나 접착형 레진 시멘트는 접착성 파괴 양상을 나타냈다. The purpose of this study was to evaluate the effect of film thickness of various resin cements on bonding efficiency in indirect composite restoration by measurement of microtensile bond strength, polymerization shrinkage, flexural strength and modulus, fractographic FE-SEM analysis. Experimental groups were divided according to film thickness (< 50 μm-control, 50 μm-T50, 100 μm-T100, 150 μm-T150) using composite- based resin cements (Variolink Ⅱ, Duo-Link) and adhesive-based resin cements (Panavia F, Rely X Unicem). The data was analyzed using ANOVA and Duncan’s multiple comparison test (p < 0.05). The results were as follows ; 1. Variolink Ⅱ showed higher microtensile bond strength than that of adhesive-based resin cements in all film thickness (p < 0.05) but Duo-Link did not show significant difference except control group (p > 0.05). 2. Microtensile bond strength of composite-based resin cements were decreased significantly according to increasing film thickness (p < 0.05) but adhesive-based resin cements did not show significant difference among film thickness (p > 0.05). 3. Panavia F showed significantly lower polymerization shrinkage than other resin cements (p < 0.05). 4. Composite-based resin cements showed significantly higher flexural strength and modulus than adhesive- based resin cements (p < 0.05). 5. FE-SEM examination showed uniform adhesive layer and well developed resin tags in composite-based resin cements but unclear adhesive layer and poorly developed resin tags in adhesive-based resin cements. In debonded surface examination, composite-based resin cements showed mixed failures but adhesive-based resin cements showed adhesive failures.

자동영상추적장치를 이용한 카드뮴 처리 Oryzias latipes의 행위독성연구

류지성,이철우,최필선,최성수,류홍일,이길철,정규혁,박광식 한국환경독성학회 1999 환경독성보건학회지 Vol.14 No.4

Changes in certain fish behaviors are known to be very sensitive indicators of sublethal exposure to environmental contaminants. Therefore, behavioral toxicity tests, if properly designed, can be very useful to assess the influence of hazardous chemicals on fish. However, quantitative analysis of xenobiotic -mediated changes in locomotor behavior in fishes are rare, due mainly to the methodological difficulties. In general, fish movement has been known to be hypertrophic or hypotrophic according to the chemicals. As a study of fish behavioral toxicities, we quatified the swimming movement of Orydas latipes using computer-automated video tracking system. Oryzias latipes was exposed to cadmium of 128 mg/L for 1 hour in a limited aquaria, then the total swimming distance, the average swimming velocity, the histogram of turning angles, and the turning frequency were analyzed. Fish treated with cadmium showed decreased swimming activities, decreased velocity, and decreased turning frequency, which means hypotrophic activity. From these results, the computer-automated video tracking system of this study seems to be a good tool for the evaluation of the potential ecotoxicological studies.

한약수치에 관한 연구(제 8보) : 오수유 탕포법 The Changes of Physico-chemical Parameter and Biological Activities by Processing of Evodia Fruit

박성환,이우정,최혁재,송보완,김동현,김남재 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

We have studied the physico-chemical change and pharmacological transformation of traditional herbal medicines with processing. Evodia fruit (EF) has processed for the purpose of detoxification and reduction of its bitter taste. There are various methods of processing EF in Oriental medicinal references. Among them, we processed EF according to the method of Sang-han theory, the most famous medicinal reference. We processed EF with washing in hot water, and then dried. Processed EFs(PEFs) were prepared according to the above procedure through repetition of 1,3,5 and 7 times respectively. The contents of weight loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, alkaloids and limonin in non-processed EF (NPEF) and PEFs were examined. The weight loss, and contents of water extract and dilute ethanol extract in PEF showed decrease in proportion to increase of washing time, while the contents of ether extract, total ash and acid insoluble ash showed little change as compared with those of NPEF. And the content of evodiamine and rutaecarpine was not changed distinctly. However, the content of limonin decreased in the final processed material in proportion to increase of washing times with hot water. And the intense of bitter taste in PEF was also reduced. These results were ascribed to the flowing-out of the water-soluble portion. The biological activities of NPEF and PEF were also investigated. In the test of DPPH scavenging effect, xanthine oxidase inhibition effect and TBA-Rs effect, PEF was more effective than NPEF in vitro. Also, both NPEF and PEF showed potent analgesic and anti-inflammatory effects against in mice. Especially, PEF by 3 times washing with hot water was more effective than other PEFs. From these results, it is considered that PEF should be prepared by 3 times washing with hot water.

조산의 역학적 연구 : 조선대학교병원 최근 6년간의 조산아 예후분석 Prognostic Evaluation of Preterm infants in chosun University Hospital from 1992 to 1997

김영호,최상준,송창훈,한세준,정혁,박상기,박종 조선의대 1998 The Medical Journal of Chosun University Vol.23 No.1

운동 유발성 횡문근 융해를 동반한 요로결석 2례

허진,최원혁,조진혁,함영희,홍정범,정성규,김현,허동 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Two men were admitted to hospital with flank pain, hematuria, which was diagnosised as ureteral stone. Elevation of aspartate aminotransferase (AST) without typical pattern of toxic hepatitis was observed. Careful history taking, several laboratory tests, abdominal and pelvis computered tomography was done. Findings from theses examinations supported the clinical diagnosis of ureteral stone complicated of exercised induced rabdomyolysis. Early recognization of rhabdomyolysis in clinical setting is important, because clinical manifestations have ranged from asymtomatic elevation of creatine kinase to acute renal failure which is a life threating medical emergency. Authors report two cases of exercised induced rhabdomyolysis initially admittied as ureteral stone managed with hydration

북한이탈주민의 외상 후 스트레스 장애와 자연살해세포 활성

김병창,정성훈,임효덕,최종혁,전정희,전양환 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.5

Objectives : The objective of this study was to evaluate an association between posttraumatic stress disorder (PTSD) and altered immunity m North Korean defectors who were more likely to develop medical conditions and other stress-related psychiatric disorders. Methods : Twenty-four North Korean defectors with PTSD and twenty-two controls without PTSD were recruited from the resettlement and training center for North Korean defectors in South Korea. Peripheral T lymphocyte subsets, natural killer(NK) cells, NK cell activity and plasma hormones (ACTH and cortisol) were obtained from all subjects. We also applied the Hamilton Rating Scale for Depression (HAM-D) and Hamilton Rating Scale for Anxiety (HAM-A) for all subjects. Results : We found NK cell activity was relatively lower than number of NK cells in North Korean defectors with PTSD. Subjects with PTSD had higher HAM-D and HAM-A scores than controls. However, there were no statistical differences in ACTH. cortisol, CD3, CD4, CD8, CD4/CD8 ratio, CD19 and CD56 between the two groups. Conclusion : These findings suggest North Korean defectors with PTSD show higher levels of depression, anxiety and impaired or weak immune function in NK cells.

친환경건축물 인증기준의 분류체계 비교 연구

이승민,박상동,신기식,최무혁 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

Energy problem of our country is very serious and the energy import is being increased continuously. To solve the problems related to energy and environment, many countries including our country have prepared and carried out various certification programs to disseminate environmentally friendly buildings. The purpose of this study is to investigate the basics and category systems of domestic green building certification criteria and to utilize it as reference materials for developing integrated criteria applicable to all buildings.