Comparison of Melanoma Subtypes among Korean Patients by Morphologic Features and Ultraviolet Exposure

( Hong Sun Jang ),( Jee Hung Kim ),( Kyu Hyun Park ),( Jae Seok Lee ),( Jung Min Bae ),( Byung Ho Oh ),( Sun Young Rha ),( Mi Ryung Roh ),( Kee Yang Chung ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.4

Background: Genetic alterations have been identified in melanomas according to different levels of sun exposure. Whereas the conventional morphology-based classification provides a clue for tumor growth and prognosis, the new classification by genetic alterations offers a basis for targeted therapy. Objective: The purpose of this study is to demonstrate the biological behavior of melanoma subtypes and compare the two classifications in the Korean population. Methods: A retrospective chart review was performed on patients found to have malignant melanoma in Severance Hospital from 2005 to 2012. Age, sex, location of the tumor, histologic subtype, tumor depth, ulceration, lymph node invasion, visceral organ metastasis, and overall survival were evaluated. Results: Of the 206 cases, the most common type was acral melanoma (n=94, 45.6%), followed by nonchronic sun damage-induced melanoma (n=43, 20.9%), and mucosal melanoma (n=40, 19.4%). Twenty-one patients (10.2%) had the chronic sun-damaged type, whereas eight patients (3.9%) had tumors of unknown primary origin. Lentigo maligna melanoma was newly classified as the chronic sun-damaged type, and acral lentiginous melanoma as the acral type. More than half of the superficial spreading melanomas were newly grouped as nonchronic sun-damaged melanomas, whereas nodular melanoma was rather evenly distributed. Conclusion: The distribution of melanomas was largely similar in both the morphology-based and sun exposure-based classifications, and in both classifications, mucosal melanoma had the worst 5-year survival owing to its tumor thickness and advanced stage at the time of diagnosis. (Ann Dermatol 26(4) 485~490, 2014)

Mycosis Fungoides 1예 보고

지미경,양기화,김병기,심상인,김선무 大韓血液學會 1984 大韓血液學會誌 Vol.19 No.1

혈관외피종 -1증례 보고-

강유미,지미경,양기화,강석진,김병기,김선무,정수일 대한병리학회 1990 Journal of Pathology and Translational Medicine Vol.24 No.2

혈관외피종 -1증례 보고-

강유미,지미경,양기화,강석진,김병기,김선무,정수일 대한병리학회 1990 Journal of Pathology and Translational Medicine Vol.25 No.5

푸드뱅크 기탁식품의 안전성 확보를 위한 HACCP 제도 적용

김철재,박현신,배현주,이지해,양일선,강혜승 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.3

The purpose of this study was to analyze the problems arising from the actual conditions of the Foodbank, and to implement the HACCP system as a solution in terms of increasing the safety of donated food within the Foodbank. In order to apply HACCP system, the entire Foodbank working process such as preparation, collection, transportation, division, and distribution was considered and analyzed to decide the application point for CCPs. Donated foods mainly consisted of processed foods, raw materials, lunch boxes, and cooked foods from mass catering establishments, which dominated over the others in terms of quantity. Cooked foods were divided into three groups based on menu-types and processing methods. Temperature, pH, and aw were measured on cooked foods, and Total Plate Count, Coliforms, E. coli, Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, and E. coli O157:H7 were conducted in order to apply a HACCP plan. Form these experiments, temperature, pH, and aw of donated food were likely contributed to microbial growth. Donated foods before HACCP implementation showed high numbers in terms of total plate count and Coliforms, both well over the acceptable standard levels. By setting the CCPs on maintenance of donated food below 10℃ and using a 75 ℃ reheating method, microbiological hazard levels were able to be controlled and lowered. From these results, it is concluded that in order to guarantee food safety, foods donated to the Foodbank must not only maintain a reasonable level of initial microbiological growth, but also must be handled properly through time and temperature controls within the Foodbank system. Furthermore, in terms of implementing the HACCP plan within the Foodbank management structure, basic food safety and sanitation measures, such as reheating facilities and various cold chain systmes such as refrigerated vehicle for food transportation are importantly needed. The training and education of Foodbank personnel and management in areas such as awareness of hygiene and safe food handling and practice are also required and necessary.

Structure-Activity Relationship of Novel Lactam Based Histone Deacetylase Inhibitors as Potential Anticancer Drugs

Yang, Jee-Sun,Chun, Tae-Gyu,Nam, Ky-Youb,Kim, Hwan-Mook,Han, Gyoon-Hee Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.6

Effectiveness of Adjuvant Chemotherapy with 5-FU/ Leucovorin and Prognosis in Stage II Colon Cancer

Sun Hee Jee,Sun Mi Moon,Ui Sup Shin,Hoe Min Yang,황대용 대한대장항문학회 2011 Annals of Coloproctolgy Vol.27 No.6

Purpose: The aims of this study were to investigate the survival results and the prognostic factors of adjuvant chemother- apy in stage II colon cancer in the sparsity of Korean data. Methods: From 1993 to 2006, 363 curatively resected pathologic stage II colon cancer patients were enrolled. Six cycles of adjuvant chemotherapy was performed: intravenous bolus 5-fluorouracil (5-FU) 500 mg/m2 with leucovorin 20 mg/m2 for 2 hours daily for 5 days, followed by a 3-week resting period (n = 308). Fifty-five patients received only curative surgery. A high risk of recurrence was defined as the presence of one or more of the following factors: T4 tumor, lympho-vascular in- vasion, perineural invasion, perforation, obstruction, retrieved lymph node < 12, and poorly differention. The median fol- low-up period was 68 months (1 to 205 months). Results: The five-year overall survival (OS) rate was 90.1%, and the five-year disease-free survival (DFS) rate was 84.7%. Among high-risk patients, the OS and the DFS rates of the treatment group were significantly higher than those of the non-treatment group (OS: 90.6% vs. 69.1%, P < 0.0001; DFS: 85.9% vs. 54.1%, P < 0.0001). Among low-risk patients, the survival results of the treatment group were also significantly superior (OS: 97.7% vs. 88.2%, P < 0.0001; DFS: 93.0% vs. 80.0%, P = 0.001). In the multivariate analysis, adjuvant chemotherapy was a significantly favorable prognostic factor for overall survival (hazard ratio, 0.41; 95% confidence interval, 0.22 to 0.75; P = 0.004). Conclusion: In our population, adjuvant chemotherapy showed superior survival to curative surgery alone and signifi- cantly reduced the risk of death. A nationwide multicenter randomized trial is needed. Purpose: The aims of this study were to investigate the survival results and the prognostic factors of adjuvant chemother- apy in stage II colon cancer in the sparsity of Korean data. Methods: From 1993 to 2006, 363 curatively resected pathologic stage II colon cancer patients were enrolled. Six cycles of adjuvant chemotherapy was performed: intravenous bolus 5-fluorouracil (5-FU) 500 mg/m2 with leucovorin 20 mg/m2 for 2 hours daily for 5 days, followed by a 3-week resting period (n = 308). Fifty-five patients received only curative surgery. A high risk of recurrence was defined as the presence of one or more of the following factors: T4 tumor, lympho-vascular in- vasion, perineural invasion, perforation, obstruction, retrieved lymph node < 12, and poorly differention. The median fol- low-up period was 68 months (1 to 205 months). Results: The five-year overall survival (OS) rate was 90.1%, and the five-year disease-free survival (DFS) rate was 84.7%. Among high-risk patients, the OS and the DFS rates of the treatment group were significantly higher than those of the non-treatment group (OS: 90.6% vs. 69.1%, P < 0.0001; DFS: 85.9% vs. 54.1%, P < 0.0001). Among low-risk patients, the survival results of the treatment group were also significantly superior (OS: 97.7% vs. 88.2%, P < 0.0001; DFS: 93.0% vs. 80.0%, P = 0.001). In the multivariate analysis, adjuvant chemotherapy was a significantly favorable prognostic factor for overall survival (hazard ratio, 0.41; 95% confidence interval, 0.22 to 0.75; P = 0.004). Conclusion: In our population, adjuvant chemotherapy showed superior survival to curative surgery alone and signifi- cantly reduced the risk of death. A nationwide multicenter randomized trial is needed.

Discovery of Orally Available Runt-Related Transcription Factor 3 (RUNX3) Modulators for Anticancer Chemotherapy by Epigenetic Activation and Protein Stabilization.

Yang, Jee Sun,Lee, Chulho,Cho, Misun,Kim, Hyuntae,Kim, Jae Hyun,Choi, Seonghwi,Oh, Soo Jin,Kang, Jong Soon,Jeong, Jin-Hyun,Kim, Hyun-Jung,Han, Gyoonhee American Chemical Society 2015 Journal of medicinal chemistry Vol.58 No.8

<P>Recently, we identified a novel strategy for anticancer chemotherapy by restoring runt-related transcription factor 3 (RUNX3) levels via lactam-based histone deacetylase (HDAC) inhibitors that stabilize RUNX3. Described here are the synthesis, biological evaluation, and pharmacokinetic evaluation of new synthetic small molecules based on pyridone-based HDAC inhibitors that specifically stabilize RUNX3 by acetylation and regulate its function. Many of the newly synthesized compounds showed favorable RUNX activities, HDAC inhibitory activities, and inhibitory activities on the growth of human cancer cell lines. Notably, one of these new derivatives, (E)-N-hydroxy-3-(2-oxo-1-(quinolin-2-ylmethyl)-1,2-dihydropyridin-3-yl)acrylamide (4l), significantly restored RUNX3 in a dose-dependent manner and showed high metabolic stability, a good pharmacokinetic profile with high oral bioavailability and long half-life, and strong antitumor activity. This study suggests that pyridone-based analogues modulate RUNX3 activity through epigenetic regulation as well as strong transcriptional and post-translational regulation of RUNX3 and could be potential clinical candidates as orally available RUNX3 modulators for the treatment of cancer.</P>

FC 2-9 : Genetic alterations among Korean melanoma patients with the evidence of tumor heterogeneity and evolution

( Hong Sun Jang ),( Mi Ryung Roh ),( Ji Hee Kim ),( Hee Su Kim ),( Byung Ho Oh ),( Jee Hung Kim ),( Kyu Hyun Park ),( Jae Seok Lee ),( Sun Young Rha ),( Kee Yang Chung ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Melanomas at different levels of sun exposure are found to have genetic alterations such as BRAF, NRAS, KIT, and GNAQ/11 mutations. Objectives: We designed this study to analyze the mutation status of primary tumors in Korean melanoma patients and to investigate the discordance rate between the primary tumors and the paired metastatic samples. Methods: Patients diagnosed with malignant melanoma from 2005 to 2012, 188 in number, at Yonsei University Health System, were enrolled for this study. Results: The most common type was acral type (n=89, 47.3%) followed by non-chronic sun damage induced type (Non-CSD type) (n=32, 17%) and mucosal type (n=31, 16.5%). The overall incidence of somatic mutation was 17.6% in the BRAF gene, 12.6% in NRAS, and KIT amplification was 28.6%. GNAQ/11 mutation in the uveal type was 66.6%. Of the non-CSD type, 41.9% had the BRAF mutation while 35.8% of the acral type had KIT amplification. Among the 43 patients who had both primary tumor and metastatic tissues, 22 patients (51.2%) showed heterogeneity, regardless of age, gender, subtype and stage. In our study, heterogeneity of the paired tumors did not affect the overall survival. Conclusion: In conclusion, the characteristics of the mutation between Korean and Caucasian melanomas are similar, but the proportion of the subtypes is distinguishable. About half of the Korean melanoma patients showed significant genetic heterogeneity which did not affect the overall survival in our study.

소와 돼지도체에서 Yersinia entericolitica의 분리 및 특성

채희선 ( Hee Sun Chae ),김주영 ( Joo Young Kim ),김지은 ( Jee Eun Kim ),양윤모 ( Yun Mo Yang ),진경선 ( Kyung Sun Jin ),신방우 ( Bang Woo Shin ),김선흥 ( Sun Heung Kim ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.2

Yersinia enterocolitica is a zoonotic agent, and to cause food poisoning. This study was carried out to get some basic information for the control of Yersinia infection. A total of 1,680 samples were collected from beef and pork carcasses from January 2006 to December 2007 in Seoul. The isolation rate was higher in pork carcass than in beef carcass. Five(0.59%) Yersinia enterocolitica were isolated from the 840 of beef carcasses, and eighteen(2.14%) were isolated from the 840 of pork carcasses. Among 23 strains, 22 were classified into biotype 1A, and one was biotype 6. In serotyping of Y enterocolitica isolates, 21 strains were untypable (UT), and 2 were O5 and O8 respectively. In PCR, Ail gene was not detected in all of 23 strains that determined non-pathogenic. In antimicrobial susceptibility test, twelve strains(52.2%) of 23 isolates showed the multi-resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty three isolates, which was digested with Xba I. the 23 isolates showed 12(A~L) PFGE type.