Ehlers - Danlos 증후군

주용진,최성문,최영자,성호석 ( Yoing Jin Joo,Sung Moon Choi,Young Ja Choi,Ho Suk Sung ) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

A Case of Ehlers-Danlos syndrome Yoing Jin Joo, M.D., Sung Moon Choi, M.D., Young Ja Choi*, M.D., Ho Suk Sung, M.D. Department of Dermatology, Clooego of Medicine, Inje University, Pusan, Korea We presented a case of Ehlers-Danlos syndrome in a 25-year old female, who had hyperextensibility of skin, skin fragility, cigarette paper-like atrophic scar, joint hyperextensibility and skeletal abnormality of scoliosis since birth. There was no family history. Microscopic and electron microscopic examinations of the skin biopsy specimens from normal appearing skin on the forearm and the site of subcutaneous nodule was compatible with finding of Ehlers-Danlos syndrome. Although the manifestations of this case including marked extensibility of skin, joint, skin fraility and atrophic scar might be in accord with type Ⅰ(gravis) Ehlers-Danlos syndrome, skeletal abnormality of scoliosis did not correspond to this type, but to type Ⅳ(hydroxylysine deficient) Ehlers-Danlos syndrome. We consider this case as a type Ⅳ Ehlers-Danlos syndrome.

고온 유리의 프레스 성형 공정 시뮬레이션

지석만,최주호,김준범,하덕식 한국 항공대학교 항공산업기술연구소 2001 航空宇宙産業技術硏究所 硏究誌 Vol.11 No.-

This paper addresses a method for numerical simulation in the pressing process of hot glass. Updated Lagrangian finite element formulations are employed for the flow and energy equations to accommodate moving meshes. The model is assumed axi-symmetric and creep flow is assumed due to the high viscosity. Commercial software ANSYS is used to solve the coupled flow and energy equations. Moving contact points as well as free surface during the pressing are effectively calculated and updated by utilizing API functions of CAD software Unigraphics. The mesh distortion problem near the wall is overcome by automatic remeshing, and the temperatures of the new mesh are conveniently interpolated by using a unique function of ANSYS. The developed model is applied to the pressing process of TV glasses. In conclusion, the presented method shows that the pressing process accompanying moving boundary can be simulated by effectively combining general purpose softwares without resorting to special dedicated codes.

연삭기용 자동 밸런싱 장치의 설계 및 평가

장홍석,최대봉,황주호,홍준희 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

The balance of high speed spindle system with high precision rotation like grinding machine is very important. Traditionally, we use trial and error method to balance the spindle. It takes much time. So we are developing the automatic balancing equipment being used in the grinding machine. The balancing head we develop is wireless. It will be used high-speed grinding machine. We use influence coefficient method to control the automatic balancer. Experiments are based on automatic and manual balancing. We perform test of the vibration filter. It helps to remove noise. The filter and experiments with automatic balancing controller show that automatic balancing control can be successfully achieved with the quick response and good stability characteristics.

땅비싸리와 좀땅비싸리의 형태적변이 및 분류

조숙경,최병희,김주환 대전대학교 이과대학 기초과학연구소 1997 自然科學 Vol.- No.-

동북아시아에 분포하는 땅비싸리와 좀땅비싸리의 분류학적 한계를 규명하기 위하여 형태적 변이와 염색체수가 조사되었다. 땅비싸리의 이명으로 취급되어 왔던 좀땅비싸리는 소엽의 모용, 꽃의 크기, 화분형태, 지리적 분포 등에 있어 땅비싸리와 뚜렷한 차이를 보였으며, 또한 염색체수가 4배체(2n=32)로 나타나, 2배체(2n=16)인 땅비싸리와 차이를 보였다. 땅비싸리와 좀땅비싸리 11 집단을 대상으로 19개의 외부형태 형질에 대한 주성분분석을 실시한 결과 두 종은 제1주성분에 의해 뚜렷이 나누어 졌는데, 전체분산에 대한 제1주성분의 기여율은 63.2%이었으며, 제1주성분은 소엽 표면의 털과 화서의 길이 등과 높은 상관성을 보였다. 이러한 결과로부터 좀땅비싸리는 땅비싸리와는 구별되어 독립된 종으로 처리되었다. 한편 큰땅비싸리는 땅비싸리의 변이의 한 형태로 판단되므로 땅비싸리의 이명으로 처리되었다. 각 종의 지리적 분포에 대한 분류학적 의의가 염색체수 변이 등과 비교논의 되었다. The morphological analysis and cytological study of Indigofera kirilowii Maxim. and Indigofera korean Ohwi were undertaken to assess the numbers and ranks of taxa that usefully could be recognized. I, koreana, previously recognized as I. kirilowiii, was separated from I. kirilowii in hairiness on leaflets, flower size, pollen exine sculptures, geographical distribution and chromasome number. Chromosome count of 2n=32 (tetraploidy) from I. koreana was obtained for the first time. The results of principal components analysis (PCA) on a matrix of 11 populations of Korea by 19 morphological characters indicated that two species could be recognized by the first principal component, contribution of which for the total variance is 63.2%. Our results supported that I. koreana could be clearly distinguished from I. kirilowii. Since the quantitative differences between the two varieties, I. kirilowii var. coreana and var. Kirilowii, were smaller, a nomenclatural synonymy was developed for them, ie, var. kirilowii.

전자현미경 In Situ Hybridization에 의한 Viral RNA의 진단에 관한 연구

김석홍,주경웅,최원기 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.2

토끼 바이러스성 출혈증의 원인체를 실험 토끼에 접종하여 증식을 유도하고 간장에서 hematoxylin & eosin 염색에서 조직학적 진단과 세포내 viral RNA의 소재를 결정하기 위해 post-unicryl 포매한 block의 절편을 사용하여 단 염색과 전자현미경적 in situ hybridization을 시도하였다. 토끼 출혈증 viral RNA의 보합 결합에 이용하는 probe는 4717에서 4800(84bases)까지 oligonucleotide를 5'말단에 biotin-CE phosphoramidite로 표지하여 사용하였다. 보합결합물의 증명은 신호 표지로서 antibiotin antibody-10mm gold를 사용하였으며, hubridization이나 증명은 기존 protocol에서 약간의 변법을 사용하였다. 0.02% glutaraldehyde에서 고정하고 unicryl resin 포매한 표본, biotinylated oligonucleotide probe, antibiotin antibody-10mm gold로 실험한 결과 증강된 신호를 얻을 수 있었다. 특히 전처리를 생략하므로써 실험 과정을 간단하게 하여 신속한 결과를 얻을 수가 있었다. 전자현미경 in situ hybridization을 통하여 토끼 출혈증 바이러스의 주요 표적은 간세포로 간염 세포의 세포질 내 미토콘드리아와 핵사이에서 immuno gold입자가 뚜렷하게 표지됨으로서 viral RNA를 증명할 수 있었다. Simple stain and electron microscopic in situ hybridization is studied and applied for the identification of rabbit haemorrhagic disease viral RNA in a unicrylated preparation of the liver after innoculation of rabbit haemorrhagic disease virus. Hybridization for detection of viral RNA in unicryl embedded tissues using complementary 84 bases oligonucleotide probe labelled by biotin CE-phosphoramidite compared with 4717∼4800 sequences of rabbit haemorrhagic disease virus, modified hybridization protocol and antibiotin antibody-l0nm gold as signal marker. The best results were obtained in 0.02% glutaraldehyde, Unicryl resin cell block, biotinylated oligonucleotide probes, antibiotin-l0nm gold. In this report, RHD viral RNA was distributed widely within the mitochondria and nucleus of liver cell by electron microscopic in situ hybridization. In situ hybridization has become a standard method for localizing DNA or RNA sequences in tissue or cell preparation. In situ hybridization is detected the virus genome in the cells and tissue as specifically compared with others nucleic acid hybridization method.

광학현미경 In Situ Hybridization에 의한 Viral RNA 증명

김석홍,주경웅,최원기 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.2

토끼 출혈증 바이러스에 감염된 조직을 10% 포르말린 고정, 파라핀 블록으로 보관했던 것으로 표본을 만들고 biotin 표지된 올리고뉴클레오티드 probe를 사용하는 in situ hybridization 기법으로 viral RNA를 조사하였다. in situ hybridization 기법은 핵산을 규명하는 다른 방법들에 비하여 신속하고 특이성이 높은 기법으로 모든 과정이 Microtrobe(Tm) capillary action system에서 1-2시간 이내에 완료된다. Viral RNA는 간세포의 세포질과 신장의 피질에서 주로 관찰되었으나, 폐조직과 신장의 수질에서는 부분적으로 적색신호가 보였다. 비록 기술적인 한계를 가지고 있지만 다른 핵산 진단방법 보다 많은 장점을 가지고 있어 조직 병리학적으로 바이러스 진단하는데 하나의 독특한 기법으로 채용되리라 기대된다. In this paper, a in situ hybridization(ISH) has been used to investigate the yield of viral RNA expression from each organ tissues. It is studied to establish a rapidly, specific diagnostic method detecting rabbit haemorrhagic disease virus(RHDV) RNA in 10% formalin-fixed, paraffin-embedded tissues of naturally RHDV-infected rabbits using oligonucleotide probe to be made by RHDV total sequences. Biotin was used as the oligonucleotide probe marker. In situ hybridization is detected the virus genome in the cells and tissue as specifically compared with others nucleic acid hybridization method. All ISH procedure of RHDV were completed to Microbrobe(Tm) capillary action system within 1-2 hours. In this report, RHDV was distributed widely in the cytoplasm of liver cell and the cortex of kidney but lung tissue and medulla of kidney were showed to positive reaction at locally. Although not entirely free of technical limitations, nucleic acid identification holds advantages over other diagnostic tests, including exquisite sensitivity, specificity, interchangeability and speed. It is expected that, in the immediate future viral nucleic acid detection will be a prominent part of the methods used in histopathology.

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.

건축물 외장의 배색 유형에 관한 연구 : 광주광역시 금남로 가로 건축물을 중심으로

이청웅,최석창,김양석,하주아 조선대학교 국토개발연구소 1997 국토개발연구 Vol.17 No.1

Since the urban environmental colors as a starting point of the research on environmental colors of the building depend on the environmental characteristics of the complex and various physical and social culture to improve the visual order of the urban landscape and don't establish its system and order, the importance of the visual effects of urban environment has been suggested through the experimental and theoretical research. It is to want to abstract the factor of surface pattern from the side of two dimentional physical factor and perceptual of architecture surface construction by concreting calssifing the criterion of analysis in to rank phase, perceptual concept, finding out basic composition factor of shape and opening architecture surface construction of design. To suggest the material of mere practical color design, this study examines the research of exterial colors of the building on the street, prescribes the characteristics of the building with other environmental factors which compose the colors and aims at suggesting the actual color design materials making the trend of colors the object of the research.

선천성 후비공 폐쇄증 : 7예의 분석

김창훈,이정권,최윤석,유신영,윤주헌 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.3

기능성 생식, 다이어트식 및 장개선제가 비만개선에 미치는 영향

전태일,전은자,김창성,이주용,안홍석,임병우,최영숙,박웅채,박동기 建國大學校附設農業資源開發硏究所 2001 農資源開發論集 Vol.23 No.-

Obesity is defined as an excess of body fat and is closely related to diabetes, hypertension and heart disease. Dietary cure has been paid attention as a method for preventing from or curing obesity. The effect of some food products developed in this laboratory, such as, functional saengsik (Health & Joy saengsik), diet food (Health & Joy diet meal) and intestine function activator (Cell-pa, commercial name) on curing and protecting from obesity were investigated. Diet program was applied to 31 adults (Males 22, Females 9) with above 120% body mass index (BMI). After diet program was applied, body weight, body fat and fat weight decreased by 1, 4.8 and 4.5% (p<0.01), HDL-cholesterol increased by 3.6% and LDL-cholesterol and atherogenic index decreased by 6.6 and 7.7% (p<0.05). Consequently, the food products developed in this laboratory effectively reduced the obesity.