http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Su Jian Liu ),( Cai Xia Yin ),( Ming Chao Ding ),( Shao You Xia ),( Qin Min Shen ),( Ji Dong Wu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.7
Berberine, a type of isoquinoline alkaloid isolated from Chinese medicinal herbs, has been reported to have various pharmacological activities. Studies have demonstrated that berberine has beneficial effects on vascular remodeling and alleviates restenosis after vascular injury. However, its mechanism of action on vascular smooth muscle cell migration is not fully understood. We therefore investigated the effect of berberine on human aortic smooth muscle cell (HASMC) migration. Boyden chamber assay was performed to show that berberine inhibited HASMC migration dose- dependently. Real-time PCR and Western blotting analyses showed that levels of matrix metalloproteinase (MMP)-2, MMP-9, and urokinase-type plasminogen activator (u-PA) were reduced by berberine at both the mRNA and protein levels. Western blotting assay further confirmed that activities of c-Fos, c-Jun, and NF-κB were significantly attenuated. These results suggest that berberine effectively inhibited HASMC migration, possibly by down-regulating MMP-2, MMP-9, and u-PA; and interrupting AP-1 and NF-κB mediated signaling pathways. [BMB Reports 2014; 47(7): 388-392]
Shen Su,Yixiao Qin,Kaiyao Yang 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.5
The steel box girder structure is widely used in many engineering practices and it is therefore necessary to study the optimization and improvement of its structure. Firstly, a small-tonnage double-girder bridge crane was used as an example, with the box section parameters for one of the main girders as the design variables and weight reduction as the objective. The mathematical model for the optimization of this validation case was developed with the strength, stiff ness and stability of the girder as the effi ciency constraints. The next step was to go through two new swarm intelligence algorithms based on animal hunting behavior, Grey Wolf Optimizer (GWO) and Whale Optimization Algorithm (WOA), as well as two classical swarm algorithms, Particle Swarm Optimization (PSO) and Genetic Algorithm (GA). The optimization models of these four algorithms were simulated and analyzed by the fi nite element method, and the simulation results were compared. The feasibility and performance of these two new swarm intelligence algorithms in the structural optimization of asymmetric eccentrically loaded box girders were verifi ed. Finally, these two new swarm intelligence algorithms were applied to the structural optimization of the main girder of a large-tonnage bridge crane.
Improved Quantum-Behaved Particle Swarm Method for Optimizing Complex Thin Plate Structure
Weitao Cheng,Yixiao Qin,Jinpeng Gu,Haibiao Gao,Yue Yan,Junle Yang,Yang Chen,Shen Su,Kaiyao Yang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.4
A large number of heavy-duty asymmetric thin-plate box girder structures exist in large equipment, and their optimization can reduce the amount of material used and increase their load-carrying capacity. A new optimization method based on the Improved Quantum-Behaved Particle Swarm Optimization method (IQBPSO) is proposed in order to efficiently solve the mathematical model for the rationalization and optimization design of structures. The penalty function and Lévy flight strategy are considered in the optimization design of the improved algorithm, thus transforming the constrained optimization problem into an unconstrained optimization problem and improving the diversity and local optimization search capability of the quantum particle swarm. A mathematical model for the optimal design of box girder section size is established with the reduction of beam cross-sectional area as the objective function and the thin plate strength, rigidity, and stability of the thin slab as the constraints. The rapid lightweight design of the thin plate box beam was achieved, resulting in a 9.6% reduction in the manufacturing cost of the thin plate box beam. The optimization results are compared with several solutions of the thin slab box beams to verify the reliability and validity of the proposed optimization method.
Chuan He,Caixia Su,Wentong Zhang,Qin Zhou,Xu Shen,Junjie Yang,Naixian Shi 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.3
Purpose: This study aimed to elucidate whether lncRNA ZFAS1 is involved in neuronal apoptosis and inflammation in temporal lobe epilepsy (TLE). Materials and Methods: Ninety-six TLE patients were recruited, and their peripheral venous blood was gathered to determine Zfas1 expression with polymerase chain reaction. Neurons were separated from hippocampal tissue of newborn SD rats, and siZfas1 or pcDNA3.1-Zfas1 was transfected into the neurons. Inflammatory cytokines released by neurons were determined, and neuronal activities were evaluated through MTT assay, colony formation assay, and flow cytometry. Results: Serum levels of Zfas1 were higher in TLE patients than in healthy controls (p<0.05). Furthermore, Zfas1 expression in neurons was raised by pcDNA3.1-Zfas1 and declined after silencing of Zfas1 (p<0.05). Transfection of pcDNA-Zfas1 weakened the viability and proliferation of neurons and increased neuronal apoptosis (p<0.05). Meanwhile, pcDNA3.1-Zfas1 transfection promoted lipopolysaccharide-induced release of cytokines, including tumor necrosis factor-α, interleukin (IL)-1, IL-6, and intercellular adhesion molecule-1 (p<0.05), and boosted NF-κB activation by elevating the expression of NF-κB p65, pIκBα, and IKKβ in neurons (p<0.05). Conclusion: Our results indicated that lncRNA ZFAS1 exacerbates epilepsy development by promoting neuronal apoptosis and inflammation, implying ZFAS1 as a promising treatment target for epilepsy.
( Mei Rong Bai ),( Jun Ni ),( Su Qin Shen ),( Qiang Huang ),( Jia Xue Wu ),( Yi Chen Le ),( Long Yu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.11
Aurora-A is a centrosome-localized serine/threonine kinase that is overexpressed in multiple human cancers. We previously reported an intramolecular inhibitory regulation of Aurora-A between its N-terminal regulatory domain (Nt, amino acids [aa] 1-128) and the C-terminal catalytic domain (Cd, aa 129-403). Here, we demonstrate that although both Aurora-A mutants (AurA-K250G and AurA-D294G/Y295G) lacked interactions between the Nt and Cd, they also failed to interact with Ajuba, an essential activator of Aurora-A, leading to loss of kinase activity. Additionally, overexpression of either of the mutants resulted in centrosome amplification and mitotic spindle formation defects. Both mutants were also able to cause G2/M arrest and apoptosis. These results indicate that both K250 and D294/Y295 are critical for direct interaction between Aurora-A and Ajuba and the function of the Aurora-A complex in cell cycle progression. [BMB Reports 2014; 47(11): 631-636]
Hua-Xing Huang,Liang-Lan Shen,Hai-Yan Huang,Li-Hua Zhao,Feng Xu,Dong-Mei Zhang,Xiu-Lin Zhang,Tong Chen,Xue-Qin Wang,Yan Xie,Jian-Bin Su 대한당뇨병학회 2021 Diabetes and Metabolism Journal Vol.45 No.6
Background: Type 2 diabetes mellitus (T2DM) is characterized by elevated fasting glucagon and impaired suppression of postprandial glucagon secretion, which may participate in diabetic complications. Therefore, we investigated the associations of plasma glucagon with estimated glomerular filtration rate (eGFR), albuminuria and diabetic kidney disease (DKD) in T2DM patients.Methods: Fasting glucagon and postchallenge glucagon (assessed by area under the glucagon curve [AUCgla]) levels were determined during oral glucose tolerance tests. Patients with an eGFR <60 mL/min/1.73 m2 and/or a urinary albumin-to-creatinine ratio (UACR) ≥30 mg/g who presented with diabetic retinopathy were identified as having DKD.Results: Of the 2,436 recruited patients, fasting glucagon was correlated with eGFR and UACR (r=–0.112 and r=0.157, respectively; P<0.001), and AUCgla was also correlated with eGFR and UACR (r=–0.267 and r=0.234, respectively; P<0.001). Moreover, 31.7% (n=771) presented with DKD; the prevalence of DKD was 27.3%, 27.6%, 32.5%, and 39.2% in the first (Q1), second (Q2), third (Q3), and fourth quartile (Q4) of fasting glucagon, respectively; and the corresponding prevalence for AUCgla was 25.9%, 22.7%, 33.7%, and 44.4%, respectively. Furthermore, after adjusting for other clinical covariates, the adjusted odds ratios (ORs; 95% confidence intervals) for DKD in Q2, Q3, and Q4 versus Q1 of fasting glucagon were 0.946 (0.697 to 1.284), 1.209 (0.895 to 1.634), and 1.521 (1.129 to 2.049), respectively; the corresponding ORs of AUCgla were 0.825 (0.611 to 1.114), 1.323 (0.989 to 1.769), and 2.066 (1.546 to 2.760), respectively. Additionally, when we restricted our analysis in patients with glycosylated hemoglobin <7.0% (n=471), we found fasting glucagon and AUCgla were still independently associated with DKD.Conclusion: Both increased fasting and postchallenge glucagon levels were independently associated with DKD in T2DM patients.
Xiang-Dang Du,Xin-Sheng Li,Gui-Qin Wang,Bao-An Cui,Su-Mei Zhang,Jian-Zhong Shen 대한수의학회 2007 JOURNAL OF VETERINARY SCIENCE Vol.8 No.3
Seventy Escherichia coli isolates recovered from diseased chickens diagnosed with colibacillosis in Henan Province, China, between 2004 and 2005 were characterized for antimicrobial susceptibility profiles via a broth doubling dilution method. Overall, the isolates displayed resistance to trimethoprim-sulfamethoxazole (100%), oxytetracycline (100%), ampicillin (83%), enrofloxacin (83%), and ciprofloxacin (81%), respectively. Among the phenicols, resistance was approximately 79% and 29% for chloramphenicol and florfenicol, respectively. Molecular detection revealed that the incidence rates of the floR, cmlA, cat1, cat2 and cat3 were 29, 31, 16, 13, and 0%, respectively. Additionally, 10% of the isolates were positive for both floR and cmlA. As these antimicrobial agents may potentially induce cross-resistance between animal and human bacterial pathogens, their prudent use in veterinary medicine is highly recommended.
Optical Characters Measurement and Simulation of 2D Cross Grating Microstructures of Butterfly Wings
Zhang, Wang,Zhang, Di,Chen, Wei-Xin,Gu, Jia-Jun,Liu, Qin-Lei,Zhu, Shen-Ming,Su, Hui-Lan,Feng, Chuan-Liang,Moon, Won-Jin 한국정밀공학회 2012 International Journal of Precision Engineering and Vol.13 No.9
In this paper, the reflectance of the butterfly wings are measured by using the microspectrophotometer as the optical character with the reflectance on several scales on the wings. Reflectance spectra from different wing scale zones with the same color are dramatically different. And it suggests that the tiny structural difference has the great influence on the reflectance property. In addition, the microstructures of the butterfly wings can be simplified to a 2D crossing grating. By simulating a two-dimensional model using rigorous coupled-wave analysis technique, the optical properties of the butterfly wings were investigated. The simulation results depend strongly on the structural parameters and refractive index. The research will reveal the mechanisms of the structural color of 2D crossing grating butterfly wings.
Optical Characters Measurement and Simulation of 2D Cross Grating Microstructures of Butterfly Wings
Wang Zhang,Di Zhang,Wei-Xin Chen,Jia-Jun Gu,Qin-Lei Liu,Shen-Ming Zhu,Hui-Lan Su,Chuan-Liang Feng,문원진 한국정밀공학회 2012 International Journal of Precision Engineering and Vol. No.
In this paper, the reflectance of the butterfly wings are measured by using the microspectrophotometer as the optical character with the reflectance on several scales on the wings. Reflectance spectra from different wing scale zones with the same color are dramatically different. And it suggests that the tiny structural difference has the great influence on the reflectance property. In addition, the microstructures of the butterfly wings can be simplified to a 2D crossing grating. By simulating a two-dimensional model using rigorous coupled-wave analysis technique, the optical properties of the butterfly wings were investigated. The simulation results depend strongly on the structural parameters and refractive index. The research will reveal the mechanisms of the structural color of 2D crossing grating butterfly wings.