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Development of EST SSR-Markers to study genetic diversity in Foxtail Millet (Setaria italica)
Jae Young Song,Yu-Mi Choi,Su Kyeung Lee,Gi-An Lee,Jae-Gyun Gwag,Myung-Chul Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Foxtail millet (Setaria italica L.) is the second most widely cultivated species of millet, especially in East Asia and is a tractable experimental model crop for studying functional genomics of millets. However, insufficient researches had been conducted about the foxtail millet germplasm and is significantly impeding its genetic improvement. We attempted to develop EST-derived-SSR (eSSR) markers and utilize them in genetic comparison of germplasm and transferability. A total of 66,027 foxtail millet EST sequences and 42,754 genomic sequence were deduced transcriptom. Approximately 42,000 single tone contigs were generated using DNAstar 5.0 software for redundancy minimization. Nearly 33% of the 14,012 unigenes contained SSRs, but primers were designed for a total of 314 microsatellites concentrating with more than 24 bp of repeats. A total of 314 primers were successfully designed with more than 24 bp of repeats. From these microsatellites, 56 primer pairs were showed polymorphism with over than 15 bp differences among 96 accessions collected from different countries. Polymorphic information content (PIC) value ranged from 0.020 to 0.700 with an average of 0.381 indicating moderate level of informativeness within these EST-SSRs markers. The EST-SSR markers developed in this study will serve as a useful source for genetic studies, such as genetic variability, transferability, association mapping, and molecular breeding
( Myung Chul Lee ),( Dong Jin Lee ),( Gi An Lee ),( Hong Jae Park ),( Jung Ro Lee ),( Yu Mi Choi ),( Su Kyeung Lee ),( Yeon Ju Jung ),( Yong Gu Cho ),( Jae Young Song ) 한국육종학회 2013 Plant Breeding and Biotechnology Vol.1 No.1
Brown rice contains beneficial phytonutrients such as antioxidants, anthocyanins and oryzanol. However, information on oryzanol contents and genetic data of Korean rice landraces are very limited. This study was conducted to investigate varietal differences of the oryzanol content, and analyze the genetic diversity of 196 Korean rice landraces. The total γ-oryzanol content of the germplasm tested varied from 9.8 to 55.9 mg 100g -1 , with an average value of 27.2 mg 100g -1 . Among the germplasm, IT007903, IT007714, IT006622 and IT006125 accessions showed γ-oryzanol contents higher than 50mg 100g -1 . Of the total γ-oryzanol components, 24-methylene cycloartenyl ferulate was the most prevalent with an average value of 29.9% followed by cycloartenyl ferulate (26.7%). A total of 396 distinct alleles were identified in genetic diversity using 46 SSR markers. Average observed heterozygosity (HO) was 0.009, while the expected heterozygosity (HE) was 0.497, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into seven clusters with a genetic distance (GD) value of 0.6. According to the phylogenetic analysis, roughly 7 clusters were divergent, 4 of which exhibited significantly different γ-oryzanol content (P<0.001). The selected accessions with identified traits can be used as donor parents in rice breeding to improve the γ -oryzanol content of rice.
Distribution of Blast Resistant Genes in Rice (Oryza sativa L.) Germplasm
Jae Young Song,Do-Yoon Hyun,Gi-An Lee,Yu-Mi Choi,Su Kyeung Lee,Yeon Gyu Kim,Myung-Chul Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Rice blast (Magnaporthe oryza B.) is one of the most widespread and devastating diseases of rice. Screening of valuable genetic resources harboring resistance genes is one of the most efficient approaches against blast disease. Because the bioassay to rice blast in the field shows high variations, this study has performed to provide DNA profiles in the accessions of diverse countries using major blast resistant genes linked markers, identified and mapped in different genotypes of rice. Because durable resistance to blast is controlled by a combination of major resistance genes, we surveyed the distribution of blast resistant genes in the 1,500 accessions using major 12 blast resistance genes linked markers. These resistant genes found that the frequency distribution of Pi-39 (66.9%), Pik-m (41.9%), Pit (40.5%), Pii (21%), Pib (19.3%), Pi-d(t)2 (12.7%), but Pita, Pita/Pita-2, Pik, Piz-t, Pi5 genes were identified in less than 10% frequency. Most of accessions contain from 1 to 4 different resistant genes. Pi39 and Pik-m genes amplified in the 69.1% and 51.7% among 356 Korean accessions, Pi39 (79.6%) and Pib (55.8%) in 113 China, Pit (80.6%) and Pib (32%) in 103 Philippines, respectively. In this study, we evaluated the blast resistance degree and the information about the distribution of rice blast resistant genes in rice germplasm. This study will help to develop effective strategies for managing rice blast disease in rice germplasm.
Lee, Jung Geon,Ahn, Curie,Yoon, Sung-Chul,Park, Jong Hoon,No, Jin Ju,Moon, Chang Suk,Song, Eun Kyeung,Hwang, Yeong Hwan,Hwang, Dae Yeon,Kim, Yon Su,Han, Jin Suk,Kim, Suhnggwon,Lee, Jung Sang,Kim, Seun 한국유전학회 2002 Genes & Genomics Vol.24 No.3
Two genetic loci, PKD1 and PKD2, have been identified as being responsible for ADPKD, but the presence of an intrafamilial clinical diversity suggests that there are disease-modifying loci. We studied the associations between polymorphism in the ecNOS gene, which are known to be associated with chronic tubulointerstitial and vascular changes, and ADPKD progression in Korean patients. A total of 112 individuals who had ADPKD and 41 normal control subjects were genotyped by PCR-RFLP, the Glu298Asp variant of ecNOS gene was discriminated with MboI. The distribution of the alleles for the ecNOS Glu298Asp polymorphism in ADPKD was: G 88 %, T 12 %, which was similar to the result of Korean population (95:5, p = 0.128) but different from Western controls (65:35, p = 0.000). No differences were found between the CRF and the non-CRF groups (p = 1.000) or the early hypertension and the normotension groups (p = 1.000). In conclusion, our results suggest that the polymorphism at Glu298Asp of ecNOS has no association with the renal progression in Korean ADPKD patients.
수용성 Dimethyl Dimethoxy Biphenylate 유도체의 간염 치료 효과
이치호,문전옥,정경욱,김수현,김남득,이성광,양희선 한국약제학회 1997 Journal of Pharmaceutical Investigation Vol.27 No.3
A water-soluble DDB derivative (Bis{2-(methylamino)ethyl}-4.4-dimethoxy-5.5', 6.6'-dimethylene-dioxy-biphenyl-2,2'-dicarboxylate, DDB-S) was synthesized and its therapeutic effects on the liver damage induced by carbon tetrachloride in rats were evaluated. Oral administration of DDB-S reduced the aspartate aminotransferase(AST) and alanine aminotransferase(ALT) activities and increased total protein and albumin contents in the serum of the carbon tetrachloride intoxicated rat. Therapeutic effects of DDB-S by intravenous injection was also investigated using carbon tetrachloride intoxicated rats. Histological studies showed that IV injection of DDB-S had improved the typical necrosis around centrilobular area in liver tissue due to the carbon tetrachloride intoxication and also prevented the elevation of liver weight/body weight ratio. IV administration of DDB-S to CCl₄-treated rats significantly decreased AST & ALT activities and also prevented the decrease of aniline hydroxylation activity of the liver. These results indicate that i.v. administration of DDB-S is very effective in recovering the liver function in CCl₄-treated rats.
참죽나무 열매의 항산화 및 Elastase, Collagenase, Hyaluronidase, α-Glucosidase 저해 효과
임수빈(Su-Bin Lim),김명욱(Myung-Uk Kim),이은호(Eun-Ho Lee),김예진(Ye-Jin Kim),조은비(Eun-Bi Cho),박경일(Kyeung-Il Park),강인규(In-Kyu Kang),조영제(Young-Je Cho) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.11
본 연구에서는 참죽나무 열매 추출물을 이용하여 항산화 및 elastase, collagenase 및 hyaluronidase 등의 효소활성 저해 효과를 평가하였다. 참죽나무 열매 추출물의 total phenolic contents(TPC)는 50% 에탄올 추출물에서 104.94 ㎎/g으로 가장 높은 추출 수율을 나타내었으며 열수 추출물의 경우 56.54 ㎎/g의 추출 수율을 나타내었다. 이를 동결건조 한 분말(Cedrela sinensis powder; CSP)의 TPC 함량은 390.73, 491.47 ㎎/g으로 높은 TPC 함량을 나타내었다. CSP의 항산화 효과를 측정한 결과 DPPH 라디칼 소거 활성은 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 72.17%와 71.38%의 소거 활성을 나타내었다. ABTS 라디칼 소거 활성을 측정한 결과 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 99.11%와 99.16%의 소거 활성을 나타내었다. PF를 측정한 결과 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 1.75와 1.91 PF를 나타내었고, TBARS는 200 ㎍/mL 농도에서 CSWP는 38.72%, CSEP는 56.95%를 나타내었다. DPPH와 ABTS 라디칼 소거 활성은 positive control로 사용한 BHT보다 높은 활성을 나타내었으나, PF와 TBARS의 경우 BHT보다 낮은 효과를 나타내었다. CSP의 elastase 저해 효과를 측정한 결과 200 ㎍/mL 농도에서 CSEP에서 85.28%를 나타내었다. Collagenase 저해 효과를 측정한 결과 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 29.38%, 56.66%의 저해 효과를 나타내었다. 따라서 CSP는 elastase와 collagenase가 관여하는 주름 생성과정을 억제할 수 있을 것으로 예상되었다. CSP의 hyaluronidase 저해 효과를 측정한 결과 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 87.80%, 88.51%의 저해 효과를 나타내었다. α-Glucosidase 저해 효과를 측정해본 결과 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 92.17%, 99.06%로, positive control로 사용한 EGCG와 유사하거나 더욱 높은 저해 효과를 나타내었다. 이상의 연구 결과를 통해 참죽나무 열매의 동결건조물은 항산화 효과, 주름개선 효과, 항염증 효과 및 α-glucosidase 저해를 통한 혈당상승 억제효과가 우수한 것으로 판단되었으며, 기능성 소재로서의 활용 가능성이 높다고 판단되었다. This study examined the anti-oxidation and inhibitory activities of extracts from Cedrela sinensis fruits on elastase, collagenase, hyaluronidase, and α-glucosidase. The total phenolics content (TPC) of C. sinensis fruits was 56.54 ㎎/g in the hot water extracts and 104.94 ㎎/g in the 50% ethanol extracts, which was the highest. The TPC in the freeze-dried powder of the hot water extracts and the 50% ethanol extracts were 390.73 and 491.47 ㎎/g, respectively. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of the C. sinensis fruits water extract powder (CSWP) and C. sinensis fruits ethanol extract powder (CSEP) were 72.17 and 71.38% at a 200 ㎍/mL concentration, respectively. The 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization of CSWP and CSEP were 99.11 and 99.16%, respectively, at 200 ㎍/mL. The anti-oxidant protection factors (PF) of CSWP and CSEP were 1.75 and 1.91 PF, respectively, at 200 ㎍/mL. The thiobarbituric acid reactive substances (TBARS) of CSWP and CSEP were 38.72% and 56.95%, respectively, at 200 ㎍/mL. The DPPH and ABTS of CSWP and CSEP were higher than those of butylated hydroxytoluene (BHT), but PF and TBARS were lower than those of BHT. The elastase inhibitory activity of CSWP was not observed but the inhibitory activity of CSEP was 85.28% at 200 ㎍/mL. The collagenase inhibitory activity of CSWP and CSEP were 29.38% and 56.66%, respectively, at 200 ㎍/mL. Therefore, CSP was considered to be a potential source of cosmetic products for the wrinkle-improving function. The hyaluronidase (HAase) inhibitory activity of CSWP and CSEP as the biological activity for functional food were 87.80% and 88.51%, respectively, at 200 ㎍/mL. The α-glucosidase inhibitory activity of CSWP and CSEP were 92.17% and 99.06%, respectively, at 200 ㎍/mL. CSWP and CSEP were similar or more effective than EGCG as a positive control. The CSP showed excellent of anti-oxidant, anti-wrinkle, and anti-inflammation activities and helped decrease the blood sugar content through the glucosidase inhibition activity. These results show that extracts from C. sinensis can be used as a functional resource with anti-oxidant, anti-wrinkle, anti-inflammation, and anti-diabetes activities.