Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

트레드밀 운동시 흡기근 테이핑이 폐활량에 미치는 영향

김민지,신수영,송월섭,조수진,최동락,황미진,황진규,박진현,김경,Dennis W. Fell 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 트레드밀 운동과 키네시오 테이핑의 효과에 따른 폐활량의 변화를 알아보기 위해 26명의 비흡연자가 참가하였으며 키네시오 테이핑을 적용하지 않은 그룹과 키네시오 테이핑을 적용한 두 그룹으로 무작위로 배정하고 각 군들을 주 3회 6주간의 트레드밀 훈련을 실시하였다. 실험 전과 후, 스파이로미터를 사용하여 키네시오 테이핑의 적용 따른 폐활량 변화 효과를 측정하였다. 이 연구의 결과를 종합해보면 키네시오 테이핑을 적용 그룹에서 적용하지 않은 그룹에 비해 FVC, FEV1에서 유의한 증가를 보였다. 이러한 결과는 건강한 성인에서 키네시오 테이핑을 적용한 경우 키네시오 테이핑을 적용하지 않고 트레드밀 운동을 한 경우보다 폐활량의 향상에 효과적이라고 생각되어진다. The purpose of this study was to investigate the variation of vital capacity(VC) according to the effects of kinesio taping with treadmill exercise. Twenty-six non-smokers were participated in this research and these subjects were randomly assigned into two groups. To measure the VC variation effects of kinesio taping, spirometer was used. The collected data were analyzed statistically by using a paired Mauchly test and repeated measure ANOVA. The results of this study were as follows; A group: treadmill with kinesio taping B group: treadmill without kinesio taping. Between A and B, there were significant differences. In the case of A group, there was 11.66% increase of VC, during 3 weeks experiment(p<.01). In the case of B group, there were significant differences, 3.35% increase of VC, during 3 weeks taping intervention(p<.01). After 6 weeks experiment, the improvement of VC shown a significant difference with intergroup (p<.05). From this result, it was revealed that treadmill exercise with kinesio taping was effective to improve VC to healthy adult than treadmill exercise without kinesio taping.

경구면역을 통한 항원 특이적 IgA 항체 합성에 있어 Cholera Toxin과 Alginate-Microsphere의 효과

송민형,유진수,권명상,성승룡,김용희,권익찬,정서영,양재명,김평현 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.3

Secretory (S-IgA) isotype antibody (Ab) is known to play an essential role in the primary defense against various infectious agents in mucosal tissue. However, it has been mostly unsuccessful in the induction of antigen (Ag)-specific IgA Ab response in this site by peroral vaccination. In the present study, we investigated the effect of cholera toxin (CI) as a mucosal adjuvant and alginates-microspheres as a carrier on BSA-specific IgA Ab response in gut-associated lymphoid tissue`(GALT). Peroral immunization of BSA plus CT conferred a great BSA-specific IgA response but IgG response on intestinal fluid (IF). In contrast, intraperitoneal immunization of BSA with Freund's adjuvant readily induced BSA-specific IgG response but IgA response in IF. Further, number of CT specific IgA-secreting cells was substantially increased in mesenteric lymph node when CT-encapsulated-VI alginates-microspheres was administered perorally. Taken together, these results indicate that peroral immunization of soluble antigen in combination of CT or microspheres significantly enhances antigen-specific IgA response in GALT.

댄스스포츠 참여가 가족응집력 및 결혼만족에 미치는 영향

송채훈,박현서,박상규,정진영 朝鮮大學校 스포츠科學硏究所 2006 스포츠科學硏究 Vol.17 No.-

Couples are happy everybody and desire that their married life is successful beginning married life, but conjugal love itself does not become sufficient condition of happy double harness. In different environment each character have and is thought in life bow as well as way that love grown-up man and woman and feel and because difference exists that act. This research drew specimen using Stratified Cluster Random Sampling after establish population to woman who get married between gwangju Chollanamdo area's dance sports program newcomers February, 2006. form the analysis of the date the following conclusion were abtaind abtaind : First, among low rank factor of family cohesive power according to incidental and age that analyze family cohesive power and marriage satisfaction according to general special quality sentimentalize relation leading person and a friend Difference that keep in mind as statistical in factor during time appeared. Second, family cohesive power by dance sports participation existence and nonexistence and result family cohesive power that analyze marriage satisfaction 4 Hawibyeons be that of that it is a car that sentimentalize relation, family support keeps in mind appear. Third, result family cohesive power that analyze dance sports participation degree and family cohesive power and relation of marriage satisfaction was expose that it is corelation that keep in mind with participation period and participation strength and Chamgibindo. Fourth, family cohesive power and low rank factor of family cohesive power that express marriage savage tribe and the strongest corelation as result that analyze relation with marriage satisfaction appeared by family economy and are since next sentimentalize relation, family, was expose by order of Chingu·time. Also, marriage satisfaction's low rank factor which display family cohesive power and the highest corelation appeared and was expose by next order of marriage, communication, authoritative marriage that is affection enemy by adaptation about married life.

관엽식물의 잎모양, 크기, 수형에 따른 시각적 인식이 뇌파변화 및 이미지 평가에 미치는 영향

송종은,엄수진,김수연,손기철,이종섭 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-

실내 환경내에서 관엽식물의 보다 효과적인 이용과 시각, 심리환경 스트레스 경감을 목적으로 관엽식물의 형태적 특성이 인간의 정신생리와 심리에 미치는 영향을 알아보고자 잎모양이 다른 아로우카리아(Araucaria excelsa)와 벤자민고무나무(Ficus benjamina), 잎크기가 다른 네프로네피스(Nephrolephis exaltata)와 스킨답서스(Scindapsus aureas), 그리고 수형이 다른 관음죽(Rhapis humilis)과 아레카야자(Chrysalidocarpus lutescens)를 선정하여 각 식물당 남녀 24명 총 72명을 대상으로 뇌파(Computerized EEG)검사, 혈압, 맥박, 시각이미지 평가를 시행하였다. 각 식물에 따른 뇌파를 비교한 결과 뚜렷한 차이를 보이지 않아 관엽식물의 관상에서 잎의 모양, 크기 및 수형이 있는 상태에서 모두 유의한 감소를 보여 스트레스 감소 효과와 안정 효과를 보인 것으로 나타났다. 그러나 각 식물간에는 유의한 차이는 없었다. 이러한 사실은 기존 연구에서 보여준 결과인 식물의 녹색에 대한 정신생리적 반응과 녹(綠)면적에 의한 뇌파변화는 인정되었지만 식물종류에 따른 반응은 중요하게 작용하지 않는 것으로 보여진다. 관엽식물에 대한 이미지는 평가는 4개의 요인 심미성, 청량감, 안정성, 질감성 인자로 구성되었다. 각 인자에 대한 식물종류간에 차이를 조사한 결과 서로 다른 특성을 보였으나 4인자인 질감성 차원에서는 차이가 없었다 이는 의식적으로 인식되는 시각적 이미지 평가요소에서 제 1인자인 심미성과 제 2인자인 청량성이 더 중요하게 작용되는 것으로 판단되었다. 앞으로 보다 명확한 효과를 검정하기 위해 녹시율(綠視率)과 여러 식물 종류를 조합한 좀 더 세밀한 비교와 함께 시간흐름에 따른 뇌파비교를 동시에 조사 분석하는 것이 필요한 것으로 판단된다.

양극산화와 절연파괴로 형성한 Ti-6Al-7Nb 합금의 산화피막 특성 평가

윤수연,이호진,배태성,송광엽 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.3

This study was performed to investigate the surface properties of electrochemically oxidized Ti-6Al-7Nb by anodic spark deposition technique. Discs of titanium alloy Ti-6Al-lNb of 20 mm in diameter and 2 mm in thickness were polished sequentially from #300 to #1,000 emery paper. They were ultrasonically washed in ethyl alcohol and acetone for 5 min and dried in a desiccator. Ti-6Al-7Nb was anodized in an electrolytic solution that was dissolved calcium and phosphate in water. The electrolytic voltage was set in the range of 170-320 V and the current density was 30 mA/cm^2. The surface oxide film was characterized by X-ray diffractometer, scanning electron microscope (SEM) and X-ray photoemission spectroscopy (XPS). The results obtained were summarized as follows; The main crystal structure of the titanium oxide was anatase type as analyzed with thin-film X-ray diffractometery. The coatings were porous with Pore diameter of 1~4 ㎛. The diameter of micropores increased with increasing the spark forming voltage. The porous coatings showed the growth of cell structure and more compact texture with increasing the voltage. Ca/P ratio in coatings was increased with increasing the voltage.

病院 放流水의 總有機炭素 濃度

崔辰洙,宋仁炫 全南大醫大 1984 전남의대학술지 Vol.21 No.3

저독성 난연제를 첨가한 에폭시 수지의 경화 동력학 및 유변학적 특성

박수진,송수완,이재락,민병각,신재섭 한국공업화학회 2004 공업화학 Vol.15 No.1

본 실험에서는 2관능성 에폭시 수지(EP)에 저독성 난연제인 tris (chloroisopropyl) phosphate (TCPP) 를 100:0~100:40 phr의 비율로 첨가한 후 이에 따른 경화 동력학과 유변학적 특성을 고찰하였다. 2EP/TCPP 시스템의 경화 활성화 에너지(E_(a))와 진화량(α)은 동적 DSC를 통하여 알아 보았으며, 유변학적 특성은 레오미터를 이용하여 등온 조건하에서 고찰하였다. 또한, 난연성 test인 UL94를 통하여 TCPP 함량에 따른 난연성의 변화를 살펴보았다. 실험 결과, E_(a)는 에폭시 수지 내에 TCPP의 함량이 증가할수록 증가하는 반면, 전화량(α)과 전환속도(d/dt)는 감소하는 경향을 나타내었다. 가교 활성화 에너지(E_(c)) 값은 구한 E_(a) 값과 유사한 경향을 나타내었으며, 이러한 결과는 TCPP의 증가로 인해 2EP 고유의 interpenetrating polymer network (IPN) 구조형성이 방해받기 때문이라 판단된다. 또한, TCPP의 함량이 30 phr 이상인 경우 난연성이 향상되었는데, 이는 TCPP 내의 난연성을 갖는 인과 염소의 영향으로 판단된다. In this work. the cure kinetics and rheological properties of difunctional epoxy (2EP) resins containing a low toxicity flame-retardant based on tris(chloroisopropyl) phosphate (TCPP) are investigated. The 2EP/TCPP content was varied within 100 : 0 ∼100 : 40 phr. The cure system are studied under the isothermal condition using a rheometer, Also, the flame resistance is determined by UL94 vertical test. From experimental results, the Ea of 2EP/TCPP system is higher than that of pure 2EP. While, the conversion (α1 and conversion rate (d/dt) are decreased with increasing of the TCPP content. The cross-linking activation energy (E_(e)) of the system is similar to (E_(a)). This is probably due to the increase of TCPP content. which obstructs the formation of interpenetrating polymer network (IPN) structure of the 2EP. Also, the improvement of flame resistance is achieved by addition of 30 phr TCPP in the 2EP/TCPP system. which can be attributed to the existence of incombustible phosphorous and chlorines in the TCPP.

황련해독탕 및 귀비탕의 발효 전후 혈소판 응집 억제 효과에 대한 비교 연구

송병정,이수진,권광일 충남대학교 약학대학 의약품개발연구소 2012 藥學論文集 Vol.27 No.-

Recently interest of fermented oriental medicines is increasing for the purpose of disease prevention. Hwangryunghaedok-tang (HR) and Guibi-tang (GB) are widely used oriental medicines in Korea. HR and GB are well known for anti-inflammatory and anti-stress effect. Glycosides in herbal medicines are transformed to aglycone via fermentation. The fermented medicines are absorbed more and have less individual variation. In this study, HR and GB were compared before and after fermentation by anti-platelet aggregation effect. HR and GB were fermented by various lactobacillus species. The anti-platelet aggregation effect of HR and GB were measured using aggregometer by optical method. Collagen (4 μg/mL) was used as a inducer of platelet aggregation. After measuring anti-platelet aggregation effect, we analysed the data using WinnonlinⓇ program to fit simple Emax model. Then Emax and EC50 values were estimated. In results, GB had little antiplatelet effects. Emax of 5 GB samples (127, 164, 402, 442, 3163) were estimated near zero and others (129, 144, 166, 693, 744) had high EC50 values (> 1500 μg/mL). In contrast with GB, HR had antiplatelet effects. HR which was fermented by Lactobacillus casei and Lactobacillus plantarum (HR-127,HR-144) were most effective sample. Emax and EC50 of HR-127 and HR-144 was 99.99%, 174.73 μg/mL and 100.00% 160.48 μg/mL, respectively. These results indicated that Guibi-tang was inappropriate as anti-platelet aggregation agent and Hwangryunhaedok-tang would be more effective after fermentation as anti-platelet aggregation agent, warranting further study.

한국어 판 구조화 임상면담도구 개발 : 신뢰도 연구

한오수,안준호,송선희,조맹제,김장규,배재남,조성진,정범수,서동우,함봉진,이동우,박종익,홍진표 대한신경정신의학회 2000 신경정신의학 Vol.39 No.2

연구목적: 정신장애의 정확한 진단과 평가는 임상에서뿐만 아니라 신뢰성 있는 연구를 위해서도 매우 중요하다. Structured Clinical Interview for DSM-IV(SCID)는 임상전문가들이 사용하는 진단도구로서, 비교적 짧은 시간에 정확한 진단을 내릴 수 있다. 본 연구는 한국어판SCID를 개발하고 그 신뢰도를 평가하기 위해 수행되었다. 방법: 국문학자가 포함된 번역위원회를 통하여 연구용판 SCID를 번역한 후, 정신과 의사 2인과 임상심리학자 1인에게 한국어판SCID 실시방법을 교육시킨 뒤 한국어판SCID를 이용한 면담의 평가자간 신뢰도 (interrater reliability)를 검증하였다. 면담 대상은 1999년 2월에서 3월까지 2개 병원 정신과에 치료중인 환자 90명(남:41명, 여:29명)이었다. 결과: 현재 장애(current disorder) 평가에서 주요 우울장애, 기분부전장애, 정신분열병, 알코올 남용 및 의존, 기타 물질 남용 또는 의존, 여러 불안장애들 및 섭식장애 등과 같은 대부분 장애의 kappa값은 .70이상으로 매우 높았다. 그 이외의 양극성 장애, 망상장애, 광장공포증, 감별 불능 신체화 장애 및 건강염려증의 kappa값도 .69에서 .40사이로 수용할 수 있는 정도였다. 평생 장애(lifetime disorder)에서는 양극성 장애(k=.69)와 감별 불능 신체화장애(k=.59)를 제외한 다른 모든 장애의 kappa값이 .70이상이었다. K-SCID 면담시 Ⅰ축 질환에 대하여 흔하게 다중 진단이 내려졌으며, 평균 진단 수는 1.5∼1.7개로 나타났다. 결론: 한국어판 SCID는 신뢰도가 높은 진단도구로 생각되며, 향후 정신질환의 정확한 진단과 임상연구에 유용하게 사용될 수 있다. Objectives: Accurate diagnosis and assessment for psychiatric disorders is crucial for research, as well as for clinical practice. Structured Clinical Interview for DSM-Ⅳ(SCID-RV) is a less time-consumimg and more accurate structured diagnostic interview form. It can be used by clinical professions and is known for a reliable diagnostic tool. Present study was conducted to develop Korean version of SCID-RV and to test the inter-rater reliability. Methods: The authors have translated original SCID-RV into Korean, and revised in parallel with sociocultural background of Korea. Ninety patients from two psychiatric hospitals, both outpatient and inpatient, were interviewed and rated independently by three raters. Results: The kappa coefficients for most of illnesses, such as major depressive disorder, dysthymia, schizophrenia, alcohol abuse and dependency, anxiety disorder and eating disorder were excellent(>0.70) in the evaluation of current disorders. And the kappa coefficients for bipolar disorder, delusional disorder, agoraphobia, undifferentiated somatoform disorder, and hypochondriasis were acceptable(>0.40) in the evaluation of current disorders. In the evalua-tion of lifetime disorders, the concordant rates of all the diagnoses except bipolar disorder and undifferentiated somatoform disorder were excellent. Lack of hierarchy in DSM-Ⅳ allows for multiple Axis I diagnoses. Mean numbers of Axis I diagnoses per subject assigned by the three raters were 1.5-1.7. Conclusions: Our findings confirm that SCID-RV yields highly reliable diagnoses. SCID-RV is recommended for accurate diagnosis in clinical practice and research on psychiatric disorders.