담배의 잎조직 배양에 있어서 기관형성과 동위효소 유형에 미치는 생장조절제의 영향

成淳基,金濬喆,成敏雄 慶尙大學校 1986 論文集 Vol.25 No.2

담배(N. tabacum cv. NC 2326) 잎조직 배양에서 기관형성에 가장 효과적인 생장조절제의 배지조성을 찾기위하여 NAA와 kinetin의 농도별 혼합처리 5종류 및 picloram과 BAP의 농도별 혼합처리 5종류의 배지에서 26℃ 조건으로 35일간 잎조직을 배양시켜 각 처리구에 대한 기관형성률을 조사하고, 6종류의 효소에 대한 동위효소 유형 및 단백질 함량은 기관형성이 가장 잘된 0.01㎎/l pocloram+0.6㎎/lBAP 처리구와 0.5㎎/l NAA+0.5㎎/l kinetin 처리구의 두 처리구의 배양체를 대상으로 조사한 결과는 다음과 같다. 1. NAA와 kinetin을 혼합처리한 5종류의 배지중 0.5㎎/l+0.5㎎/l kinetin 처리구에서 shoot와 뿌리가 각각 100% 및 50%로서 가장 높은 기관형성률을 나타냈다. picloram과 BAP 혼합처리구에서 callus형성률은 평균 6%로 callus 형성에는 효과적인 배지는 아니었으나 기관형성률은 높게 나타났다. 그 중 0.01㎎/l pocloram+0.5㎎/lBAP 처리구에서 shoot와 뿌리가 각각 100% 및 90%의 형성률을 나타내어 기관형성에 가장 효과적이었다. multiple shoot 형성에는 picloram과 BAP 혼합배지가 NAA와 kinetin 혼합배지보다 더욱 효과적임을 알 수 있었다. 2. 배양제의 단백질 함량은 기관형성 과정에서 배양이 진행됨에 따라 두 처리구에서 모두 감소하였으나 picloram과 BAP 혼합처리구는 NAA와 kinetin 혼합처리구보다 낮았다. peroxidase의 활성은 이와 반대로 picloram과 BAP 혼합처리구에서 약 8배 높게 나타났다. 3. peroxidase와 malate dehydrogenase 동위효소들의 활성은 shoot가 형성됨에 따라 두 처리구 모두 증가하였으나, lactate dehydrogenase 동위효소들은 두 처리구 모두 감소하였다. 이러한 경향은 두 처리구 중에서 picloram과 BAP 혼합처리구에서 더욱 뚜렷하였다. 그러나 esterase, acid phosphatase, aspartate aminotransferase 동위효소들의 활성은 shoot 형성중에 두 처리구 모두 뚜렷한 변화가 없었다. 4. 6종류의 효소에서 분리된 동위효소의 수는 두 처리구를 합쳐 peroxidase에서 12개, acid phosphatase에서 6개, aspartate aminotransferase에서 9∼13개, lactate dehydrogenase에서 3∼5개, esterase에서 10∼16개가 각각 확인되었다. Tabaco (Nicotiana tabacum cv. NC 2326) leaf tissues were cultured on Murashige and Skoog medium containing various concentrations and combinations of some growth regulators at 26℃ for 35 days. The obtained results were summarized as follow; 1. Organ formation was 100% in shoot and 50% in root on the medium containing 0.5㎎/l NAA and 0.5㎎/l kinetin. On the medium containing 0.01㎎/l picloram and 0.5㎎/l BAP, organ formation displayed 100% multiple shoot formation and 90% root formation without callus formations. Multiple shoot formations were enhanced more by picloram and BAP groups than by NAA and kinetin groups. 2. During the shoot formation, protein content in the cultures on the medium containing picloram and BAP was lower than NAA and kinetin groups, but peroxidase activity in the former medium was higher about eight times than that in the latter medium. 3. The activity of anodic peroxidase and malate dehydrogenase isozyme increased during the shoot formation, whereas that of lactate dehydrogenase isozyme decreased. However the activity of esterase, acid phosphatase, and aspartate aminotransferase did not change. 4. All the isozymes were separated with 12 bands in peroxidase, 6 in acid phosphatase, 9∼13 in malate dehydrogenase, 3∼5 in lactate dehydrogenase, 10∼16 in esterase, 6 in aspartate aminotransferase during the shoot formation.

대학 전산 정책의 방향설정 및 추진계획에 관한 연구

홍상은,이병수,조충호,이상정,주경수,안종근,황순기 순천향대학교 1994 논문집 Vol.17 No.1

It has been rapidly given to the importance of the embodiment of the efficient computer systems in university education. Since the recent trend of the adoption of the openess policy in university education systems significantly affects on the realization of the embodiment of the efficient computer systems which is very essential to promote the international competition in many areas of university education. So it is greatly needed that our Soonchunhyang university must have the following principles for the embodiment of the efficient computer systems. (1) It is required that the appropriate computer education and the good quality of research environment must be given. (2) The establishment of the synthetic computer network system for efficient the university administrations is needed. (3) It is needed to establish the computer system for the consideration of the future performance. (4) It is also needed to improve the organization and the administration systems of the university computer center. (5) It is required on the establishment of the local area network(LAN) systems in the Soonchunhyang university, and the LAN must be connected to other outer national and international computer systems. And we propose the following requirement for the establishment of the convienent computer systems in our university and we have been greatly though about the significant change of the environment in university computer system. [1] The subject of the basic computer must become as the required subject for the all students in the curriulum. [2] The new computer systems both in hardware and software must be introduced. [3] It is needed to the change of the present name of the university computer center into the university information and computer center. And its organizations must be furthur developed [4] The training center for the university emploee is also needed. [5] The establishment of the database systems for the automtion of the university administation is needed. [6] It is strongly required on the establishment of the local area network system in the Soonchunhang university to connect the Bitnet and the Internet through the Hankook communication. [7] The computerization for the central library is needed. The indepent local area network system for the library is also needed to establish the own database system. Finally we hope that the LAN will be connected to the national wide library computer system.

신뢰성 기준을 적용한 용수공급능력의 해석 : 낙동강유역을 중심으로 for the Nakdong River Basin

차상화,지홍기,이순탁 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12

In general, the evaluation of water supply capacity is important factor to establish various establishment of water resource supply plan include water resource security and determination of dam's mass. But former researchs about estimation of water supply capacity were lack in continunity of evaluation basis, and didn't excute analysis on reliability criteria also. In this study, Nakdong river was selected for study basin, and then water supply capacity was analyzed by HEC-5 model using indentical reliability criteria.

상황요인, 균형성과표, 그리고 성과에 관한 실증연구 : An Empirical Investigation

김순기,김상우,탁은 서강대학교 경영연구소 2005 서강경영논총 Vol.16 No.1

자동화학분석기 Hitachi 736-20과 Parallel^(�)의 비교 검토

김순기,김용남,박용성,이기문 대한임상병리사협회 2001 대한임상병리사회지 Vol.21 No.1

충남서부지역의 콤바인 이용 및 구입성향조사

황성만,이승기,권순홍,유선대 공주대학교 자원과학연구소 2000 資源科學硏究論文集 Vol.8 No.-

The actual state of using, purchasing of combines have been investigated among the farmers who possess the three row and four row type combines of self thresher in Chung-Nam Province to present reasonable maintenance and utilization of combines. And then the condensed results are as follows. 1. It was found that 25.9% of surveyed farmhouses cultivated rice over 4.5ha which brings average income presented by the Department of Agriculture and Forestry. And 85.2% of farm houses had higher income than the national average 23,488,000 won in 1997. 2. It was surveyed that the working hours a day and working days a year are 9.26 hours and 19.2 days. And they have the charge of 22.8ha a year. 3. It was shown that the most frequent breakdowns happened to pretreating and reaping in 44.4% and the frequency of the annual repair and maintenance is 4.1 times. And they think the endurance years of 6.5 is proper. 4. It was surveyed that 33.3% of the farmers were motivated to buy combines for their own management and supporting others, 22.2% for the difficulties of timing, 9.3% of them for the possibility of purchasing in cheap price. 5. It was found that when they decided to buy combines, 61.1% of farm houses had experiences of analyzing for their management and 38.9% of them didn't. Also the more combines they purchase, the larger and higher qualities of combines they tend to choose. 6. It was shown that 25.9% of surveyed farmers were satisfied with their combines and 74.1% of them weren't. 47.5% of dissatisfaction resulted from high price, 27.5% from frequent breakdowns, 12.5% from rough after service. 7. I think it is necessary for the related authorities to give public information and education for maintenance, management analysis, keeping of combines to make farmers purchase and use them reasonably. Also overall supporting policies are needed for research and development of highly efficient combines and the ways of securing cultivating areas with the spread of large size combines.

신원방우황청심원의 뇌허혈 및 중추신경계에 미치는 영향

조태순,이선미,이은방,조성익,김용기,신대희,박대규 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract-In order to investigate pharmacological properties of New Wonbang Woohwangchungsimwon Pill(NSCH) and Wonbang Woohwangchungsimwon Pill (SCH), the effects of NSCH and SCH on cerebral ischemia and central nervous system were compared. Cerebral ischemia insult was performed using unilateral carotid artery occlusion in mongolian gerbils. The histological observations showed preventive effects of NSCH and SCH treatments with ischemia-induced brain damage. The ATP in brain tissue was decreased in vehicle-treated ischemic gerbils. This decrease was prevented by SCH treatment. In contrast to what was seen with ATP, lipid peroxide were elevated in vehicle-treated ischemic gerbils. This elevation was inhibited by NSCH and SCH treatments. While NSCH and SCH had no effects on the hexobarbital-induced sleeping time, they prevented the seizures induced by eletric shock and strychnine. NSCH and SCH showed sedative effect in rotarod and spontaneous activity test. Furthermore, NSCH and SCH showed anti-stress effect. Our findings suggest that the pharmacological profiles of NSCH on cerebral ischemia and central nervous system are similar to those of SCH.

CoO단결정의 유전성과 전기 전도도 : Dielectric Properties and Electrical Conductivity

金祥洙,金英鎬,趙順基 明知大學校 自然科學硏究所 1983 자연과학논문집 Vol.1 No.-

Dielectric constant ?? dielectric loss ??, and conductrivity σof single crystal CoO as a function of frequency from 10²to ?? cps and temperature from 90K to 600K have been determined. The dielectric constant ?? is constant in the whole frequency range at 25℃. And the dielectric constant increases exponentially with temperature. Using modified Debye equations, thermal activation energy for ?? have been obtained 0.35 eV for temperature above about 300K. The plot of logo VS1/T gives straight line from 300K to 600K with activation energies 0.46 eV and 0.12 eV.

상아질 결합제가 컴포머의 불소유리에 미치는 영향에 관한 연구

윤여상,김종수,권순원,김용기 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.2

본 연구의 목적은 와동 충전시 충전재와 지질 사이에 개재되는 상아질 결합제가, 수복물에서 유리되는 불소가 와동 벽으로 침투하는 과정에 어떠한 영향을 주는지를 조사함이었고, 부가적으로 레진 강화형-글라스 아이오노머 시멘트의 접착에도 상아질 결합제를 도포하는 것이 체질과의 결합력을 강화시킬 수 있는지에 대해 평가하고자 하였다. Fuji II LCR와 Dyract APR를 선정하여 상아질 결합제의 도포 여부에 따른 불소 유리량 측정과 전단 결합 강도도를 비교분석하였으며, 치질 내로의 불소 침투 양상은 교환 시기에 있는 재2유구지에 Fuji II LCR와 Dyract APR를 충전하고 3주내에 발거하여 EPMA필 분석하였다. 상아질 결합제는 불소 유리량을 현저하게 감소시키는 것으로 나타났으며(p<0.05) Fuji II LCR의 경우 상아질 결합제의 도포가 결합강도를 증가시키지 못하였다. EPMA분석결과 상아질 결합제는 충전재로부터 유리되어 나오는 불소가 치질 내로 확산되는 것을 방해하는 것으로 확인되었다. It is well known that glass-ionomer cement and compomer can release fluoride which can inhibit the progresion of dental caries. The purposes of thins study were to evaluate whether the fluoride from fluoride-containing ailing materials can penetrate the dentin bonding adhesive and the dentin bonding adhesive can increase the bond strength of resin-modified glass lonomer. The amount of fluoride release from resin-modified glass lonomer and compomer was measured during 28 days of period find statistically analyzed by t-test. The bond strength of each material with/without dentin bonding adhesive was measured and also statistically analyzed by t-test. The distribution of fluoride from each material into the tooth was explored by electron probe microanalysis system (EPMA). The expenmental teeth used were second primary molars about to exfoliate. The amount of fluoride from each material was diminished by dentin bonding adhesive during 28 days(p<0.05) and the bond strength was not increased by dentin adhesive in resin-modified glass ionomer. The bond strength of group VI was not detectable. The distribution of fluoride from earth material into teeth was according to dentino-enamel junction and dispersed into pulp from pulpal floor. The widest distribution was shown in resin-modified glass ionomer cement filled without the application of dentin bonding adhesive.

신원방우황청심원액의 뇌허혈 및 중추신경계에 대한 약효

조태순,이선미,이은방,조성익,김용기,신대희,박대규 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract-In order to investigate pharmacological properties of New Wonbang Woohwangchungsimwon Liquid(NSCL) and Wonbang Woohwangchungsimwon Liquid(SCL), the effects of NSCL and SCL on cerebral ischemia and central nervous system were compared. Cerebral ischemia insult was performed using unilateral carotid artery occlusion in mongolian gerbils. The histological observations showed a preventive effect of NSCL and SCL treatments with ischemia-induced brain damage. The ATP in brain tissue was decreased in vehicle-treated ischemic gerbils. This decrease was prevented by NSCL and SCL treatment. In contrast to what was seen with ATP, lipid peroxide were elevated in vehicle-treated ischemic gerbils. This elevation was inhibited by NSCL and SCL treatments. While NSCL and SCL had no effects on the hexobarbital-induced sleeping time, they showed sedative effect in rotarod and spontaneous activity test. NSCL and SCL prevented the seizures induced by electric shock and strychnine, but the effect of NSCL was less than that of SCL. Furthermore, NSCL and SCL showed anti-stress effect. Our findings suggest that the phamacological profiles of NSCL on cerebral ischemia and central nervous system are similar to those of SCL.