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      • 콩 및 당의 종류의 달리한 매실 콩아이스크림의 품질특성

        이숙영,김아영,유지영,이중기,이향섭 중앙대학교 생활과학연구소 2003 생활과학논집 Vol.17 No.-

        The purpose of this experiment was to compare the quality characteristics of soy ice creams prepared with soy protein isolate(SPI) or Jinpum-soybean, and different kinds of sweeteners of maltitol and sucrose, xylitol+sucrose, or malitiol. The viscosity of the ice cream mix containing xylitol+sucrose appeared the highest, and that from Jinpum-soybean was higher than that from SPI. The overrun of soy ice creams containing xylitol+sucrose was the highest, and the next was the samples containing maltitol+sucrose. The overrun values of Jinpum-soybean samples appeared higher than those of SPI samples. As for the case of the SPI samples, the melt-down percent was the highest in the sample of maltitol addition, and the next were maltitol+sucrose addition, xylitol+sucrose addition in order. The melt-down percent of Jinpum-soybean was the highest in the sample of maltitol+sucrose addition, and the next were maltitol addition, xylitol+sucrose addition in order. The higher the viscosity of samples, the lower the melt-down percent. In sensory characteristics, the beany flavor, flavor, mouthfeel, overall quality of Jinpum-soybean samples were evaluated better than those of SPI in general. The samples containing xylitol+sucrose evaluated better in beany flavor, mouthfeel, icy feel, and overall quality. The quality characteristics of the samples prepared with Jinpum-soybeans and xylitol+sucrose were evaluated the best.

      • T-2 Toxin이 병아리 비장세포의 유전질 발생에 미치는 영향

        전향숙,정덕화,이서래 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-

        시험관 내에서 T-2 toxin이 병아리 비장세포의 blastogenesis에 미치는 영향을 살펴본 결과, B-cell mitogen인 lipopolysaccharide및 T-cell mitogen인 concanavalin A자극에 대해 T-2 toxin의 농도가 증가함에 따라 억제정도가 증가하는 경향을 나타내었다. 노출시기를 달리하여 T-2 toxin을 투여한 병아리 비장세포에서 mitogen 자극에 대한 반응을 알아 본 결과, 부화하기 전과 후에 계속 T-2 toxin에 노출시킨 실험군은 가장 영향을 많이 받은 것으로 나타났고 부화전 혹은 부화후 어느 한 시기에만 T-2 toxin에 노출된 실험군은 비교적 영향을 적게 받는 것으로 나타났다. The effects of T-2 toxin of mitogen-induced blastogenesis of chick splenic cells were investigated. The[^3H]thymidine incorporation in splenic cells stimulated by lipopolysaccharide and concanavalin A were equally inhibited as the concentration of T-2 toxin was increased. The effective dose of T-2 toxin causing a 50% reduction of[^3H]thymidine incorporation was inbetween 1.0 and 5.0 ng/㎖ for both mitogens. Mitogen-induced blastogenesis in chick splenic cells showed differences among experimental groups with different exposure time of T-2 toxin, exhibiting the most inhibition in the experimental group exposed to T-2 toxin at both embryonic and chick periods.

      • Effects of Conomitant Micro-organisms in a Culture Medium of Entamoeba histolyica on the Establishment of Abscesses in Hamster Livers

        김경래,박 현,정윤자,김숙향,소진탁 INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1991 YONSEI REPORTS ON TROPICAL MEDICINE Vol.22 No.1

        통상적인 이질아메바 배지내에는 분변에서 유래된 각종 세균 특히 장내세균등이 배지에 첨가한 항균제에 의하여 차츰 감소되기는 하나 대부분의 경우 몇 가지 종류는 그대로 남아 있어 특수한 무균처치(axenization)를 하지 않을 경우 거의 영구적인 공존 미생물(concomitant micro-organism)로서 이질아메바 배지에서 없어지지 않는다. 본 연구는 현재 실험실에서 계대 보관중인 10개 이질아메바를 대상으로 그 배지 modified Boeck-Drbohlab's diphasic medium으로부터 6가지 공존 세균을 분리하였다. 즉 Escherichia coli, Klebsiella pneumoniae, Acinetobacter sp., Aeromonas hydrophilus, unidentified gram negative Diplococcus, glucose-gas negative but not Salmonella등 6개 종류를 검출하였는데 그중 E. coli 및 D. pneumoniae와 또 TTY-SB monophasic 배지내의 이질아메바 공존 원충인 Crithidia sp.를 재료로 하여 그들이 이질아메바의 간농양 형성에 미치는 영향을 실험적으로 검토한 바 그 얻어진 성적을 요약하면 다음과 같다. 1) Escherichia coli는 간조직내에서 약 1개월 또는 그 이상 생존 가능하다 단독으로는 병변을 야기할 수 없었고 이질아메바와 공존시 아메바성 간농양 형성을 조장하였다. 2) K. pneumoniae는 단독으로는 간조직에 농양을 형성하지 않았으나 아메바성 간농양 형성은 조장하였다. 3) Crithidia sp.는 간조직에서 병변을 야기하지 않았고 6시간 이내에 소실되나 이질아메바와 섞여 간조직에 주입할 경우 아메바성 농양 형성을 조장하였다. 4) 무균적으로 배양된 이질아메바는 간조직에 주입하여도 농양형성을 볼 수 없었으나 배지에 Escherichia coli를 혼합한뒤 6시간 경과된 아메바성 간농양 형성이 가능하였다. 이상의 성적으로 미루어 이질아메바 배지내 공존 세균과 무균배지내 이질아메바는 각각 단독으로는 간농양을 형성할 수 없으나 특수미생물과 이질아메바가 일정한 시간 공존하면 세균이 이질아메바의 병원성을 조장하여 아메바성 간농양이 더 촉진되는 것으로 생각된다.

      • KCI등재후보

        성인 암환자의 질병 특성에 따른 증상발생 정도

        허혜경,이은현,이원희,소향숙,정복례,강은실 성인간호학회 2002 성인간호학회지 Vol.14 No.3

        Purpose: This study was to investigate symptom occurrence related to the disease characteristics of patients with cancer. Method: A total of 301 patients with cancer participated in this study. The participants were recruited from University Hospitals located in Seoul, Wonju, Kwangju, Daegu, and Pusan. Data collection was performed by using a questionnaire on symptom occurrence. The obtained data was analyzed using SPSS computer program that included descriptive statistics, Pearson correlation, one-way ANOVA, and t-test. Result: The mean score of fatigue was the highest (3.24), followed by loss of appetite, lack of concentration, change in appearance, pain, insomnia, change in bowel pattern, nausea/vomiting, coughing, and dyspnea. Most symptoms were significantly correlated with each other. The level of symptom occurrence in patients with lung cancer or cervix cancer was significantly higher than the level in patients with stomach cancer. Patients receiving radiation therapy or a combined therapy of radiation therapy and chemotherapy experienced significantly higher level of symptom occurrence significantly higher level of symptom occurrence than those receiving chemotherapy only. Also, female patients experienced higher level of symptom occurrence than male patients did. Conclusion: The sites of cancer, types of treatment, and gender influence the level of symptom occurrence of patients with cancer. Thus, these variables should be considered when assessing and planing for symptom occurrence of patients with cancer.

      • Construction of Yeast Vectors Potentially Useful for Expression of Eukaryotic Genes as β-galactosidase Fusion Proteins

        Chung, Kyung-Sook,Choi, Won-Ja,Lee, Hee-Won,Kim, Kyu-Won,Yoo, Hyang-Sook 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

        By both in uitro hydroxylamine mutagenesis of the wild type 3-phosphoglycerate kinase gene(PGK)promoter DNA and insertion of the leu2-d gene. we have created yeast expression vectors potentially useful for production of eukaryotic genes in yeast. The guanine(G)to adenine(A)change at the -3 position from the ATG start codon of the PGK promoter-based vector rendered a 6∼7 times elevated expression of the ada?? eukarryotic gene. and insertion of the leu2-d gene in the vector containing the mutated PGK promoter further enhanced the expression of the gene When expression of the AIDS virus HIV1-gogP17 gene in a lacZ fusion from was examined with this new vector a 15 times higher level of expression than that from the original PGK promoter was observed. Northem and Southem analysis showed that this elevated expression is due to the production of a high copy number of mRNA by leu2-d gene functioning and by efficient translation of the produced mRNA thus the vector that contained the A at the -3 position from the ATG start codon in the promoter region and the leu2-d gene shows increased expression capability and will be potentially useful for production of eukaryotic genes in yeast.

      • KCI등재SCISCIE
      • Construction of Yeast Vectors Potentially Useful for Expression of Eukaryotic Genes as ${\beta}$-galactosidase Fusion Proteins

        Chung, Kyung-Sook,Choi, Won-Ja,Lee, Hee-Won,Kim, Kyu-Won,Yoo, Hyang-Sook Korean Society for Biochemistry and Molecular Biol 1996 Journal of biochemistry and molecular biology Vol.29 No.4

        By both in vitro hydroxylamine mutagenesis of the wild type 3-phosphoglycerate kinase gene (PGK) promoter DNA and insertion of the leu2-d gene, we have created yeast expression vectors potentially useful for production of eukaryotic genes in yeast. The guanine (G) to adenine (A) change at the -3 position from the ATG start codon of the PGK promoter-based vector rendered a 6~7 times elevated expression of the adjacent eukaryotic gene, and insertion of the leu2-d gene in the vector containing the mutated PGK promoter further enhanced the expression of the gene. When expression of the AIDS virus HIV1-gagP17 gene in a lacZ fusion form was examined with this new vector, a 15 times higher level of expression than that from the original PGK promoter was observed. Northern and Southern analysis showed that this elevated expression is due to the production of a high copy number of mRNA by leu2-d gene functioning and by efficient translation of the produced mRNA. Thus, the vector that contained the A at the -3 position from the ATG start codon in the promoter region and the leu2-d gene shows increased expression capability and will be potentially useful for production of eukaryotic genes in yeast.

      • 병원 간호조직의 효과성에 영향을 미치는 간호관리 기능에 관한 연구

        박청자,박정숙,김정남,고효정,권영숙,박영숙,양진향,최선희 中央醫學社 1993 中央醫學 Vol.58 No.2

        Tie purpose of this study was to identify the nursing management function which influence the effectiveness of nursing organization in the hospital. The data of this study were collected by self-reported questionnaire composed of 4 point rating scale to measure the ideal level and the performance level of the nurses role. For the analysis of the data, Percentage, MANOVA and ANOVA were used, Scheffe test by post test. The respondents were 379 nurses in 7 general hospitals in Taegu. Data was administrated from October 4 through 15, 1992. The results were as follows; 1. The average mean score for the ideal level was 3.77 with a maximum possible score 5 points. The highest mean score was leading and the lowest mean score was controlling. The average mean score for the performance level was 2.29, the highest mean score was staffing, leading, controlling, organization. In the desirable nursing performance, planning was found the highest response above head nurse, staffing was found the highest response above supervisor. 2. In the analysis of the relationship between the ideal level and the performance level, significant difference was found in the position, age, career, marital status, educational level, occupation satisfaction. 3. In the analysis of the relationship between the ideal level and the general characteristics of was found that career, marital status, educational level, occupation satisfaction were the characteristics to be significant in the all function, planning, organization, staffing, leading, controlling, that position and age were the characteristics to be significant in the planning, organization, staffing, controlling. In the analysis of the relationship between the performance level and the general characteristics was found that position, age, career, occupation satisfaction were the characteristics to be significant in the all function, educational level was the only characteristic to be significant in the planning, organization, leading, controlling, marital status was the only characteristic to be significant in the planning, leading, controlling.

      • KCI등재후보

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