有機鹽素系 化合物(γ-BHC)을 投與한 마우스의 脂肪代射에 관한 硏究

金炳洙,尹蓮淑 梨花女子大學校 韓國生活科學硏究院 1976 韓國生活科學硏究院 論叢 Vol.17 No.-

正常群, γ-BHC低用量 投與群, γ-BHC中等用量 投與群, 및 γ-BHC高用量 投與群에 對하여 Liver lipid classes의 定性分析, liver total lipid量 및 liver total cholesterol量을 測定하여 γ-BHC의 肝臟脂肪代謝에 미치는 영향을 檢討하였다. 測定方法은 Thin-layer chromatography와 吸光度測定法을 使用하였다. 그結果를 綜合하면 다음과 같다. 1) 正常群과 γ-BHC投與各群들의 liver lipid chasses는 同一하였다. 2) Liyer total lipid量은 正常群에 比해 γ-BHC 投與各群들에서 增加하였다. 3) Liyer total chalesterol量은 正常群에 比해 γ-BHC 投與各群들에서 增加하였다. The qualitative analysis of mouse liver lipid chasses was carried out by TLC. The total mouse liver lipid was determined by spectrophotometry. The total mouse liver cholesterol was determined by spectrophotometry. The above experimentes were carried out at non, low, medium and high concentration γ-BHC administrated mouses. The results were as follows: 1) The lipid classes of mouse liver were the same in normal and γ-BHC administrated mouses. 2) The total lipid of mouse liver was increased in γ-BHC administrated mouses. 3) The total cholesterol of mouse liver was increased in γ-BHC administrated mouses.

항공사 객실승무원 단체협약 연구

김수련,김강식 한국항공대학교 경영연구소 2003 경영연구 Vol.10 No.1

본 연구는 항공사 객실승무원의 근무환경 개선과 근로생활의 질 향상을 위해 외국항공사의 단체협약을 통하여 그 방안을 모색하고자 하였다. 첫째 근무환경개선을 위해 단체협약에 각 직종의 근무특성을 명시한 근무협정서가 명시되어야 한다. 둘째 객실승무원의 합리적인 근무할당 및 승무원운영방안이 모색되어 합리적인 근무시간과 휴식시간의 분배가 있어야 한다. 셋째 동기부여 및 책임자 권리를 위한 적절한 보상과 근무시간에 대한 규정이 필요하다. 넷째 안전에 관한 보호규정이 고려되어야 한다. 다섯째 의사결정과정에의 참여와 협력증진에 관한 제도 보장 및 개선으로 근무할당 및 근로조건의 개선, 개발에 객실승무원의 참여의 길을 열어 노사 공동의 이익을 위한 방안이 필요하다. This study is for development of working condition and circumstances of flight attendants of Airline after review their agreement of union and through it. First, to protect and avoid the consumable confliction in between of company and union, agreement of union of flight attendant must be included with specified job description and clear statement of clarify. Second, Systematically organized timetable for working & rest schedule for flight attendant to arrange and development their labor efficient. Third, to inspire responsibility and motivation for their job and for their rights of job, prepare the adequate regulation for working hour management and compensation. Fourth, to increase their mental stability, set the security protection systems, regulation and its enforcement by inaction. Fifth, Necessary of channels and system for to negotiation of working condition and circumstances reflect and its improvements and development for bath benefit of company and labors.

전립선 기질세포의 증식과 COX-2 발현에 대한 프로게스테론의 영향

정수련,김성한,최이화,박지은,전은미,강영진,이광윤,최형철 영남대학교 의과대학 2006 Yeungnam University Journal of Medicine Vol.23 No.1

전립선비대증은 노인 남성에서 흔히 유발되는 질환이며, 노화가 진행될 수록 빈도가 높아지는 특징을 가진다. 이 질환의 원인은 전립선기질세표의 과도한 증식으로 유발된다고 알려져 있지만 그 자세한 기전에 대해서는 잘 알려져 있지 않다. 전립선비대증에서 progesterone 수용체 양성 세포가 다른 전립선 종양에 비해서 많고, progesterone은 testosterone에서 DHT로 전환되는 것을 감소시키는 역할을 가진다고 알려졋다. 또한 남성 전립선 평활근의 과증식에 의한 질환이므로 평활근 세포의 증식과 관련성이 있다고 보고된 COX-2의 전립선비대증에 대한 영향에 대한 연구가 필요하다. 전립선 기질세포에 progesterone을 3일간 투여하여 배양한 경우 기질세포 증식은 차이가 없었다. Progesterone을 단독 또는 DHT와 같이 투여한 기질세포에서 남성호르몬 수용체 mRNA 발현은 비처리군과 비교하여 유의한 차이가 없었다. 또한 progesterone과 DHT 동시 투여에 의한 COX-2 mRNA 발현에도 차이가 없었다. 그러나 progesterone에 의한 남성 호르몬 수용체와 COX-2 단백 발현에서는 대조군과 비교하여 유의하게 감소시켰다. 이상의 결과는 progesterone은 남성호르몬 수용체에 대해 전사 후 반응 (post-transcriptional response)에 효과를 나타내어 남성호르몬 수용체 발현을 감소시키는 작용은 가지며, COX-2 발현 억제효과를 나타내므로 전립선비대증의 치료에 이용될 수 있을 것으로 사료된다. Background: Benign prostatic hyperplasia (BPH) is the most common benign tumor in older men; the etiology of this disease remains poorly understood. Testosterone and dihydrotestosterone (DHT) both act as androgen via a single androgen receptor. Testosterone is converted to DHT by 5α-reductase in prostatic stromal cells. Progesterone has been reported to inhibit DHT conversion; howevwe, its effect on prostatic stromal cells remains to be elucidated. Materials and Methods: In this experiment, we investigated the effect of progesterone on androgen receptor expression induced by DHT. We also tested the effect of progesterone on cyclooxygenase-2 (COX-2) expression, as well as prostate stromal cell proliferation using the cell count kit-8. Results: Progesterone did not cause an increase of prostate stromal cell proliferation. The mRNA expression of the androgen receptor and COX-2 were not changed by progesterone; the expressions of androgen receptor and COX-2 proteins were decreased by progesterone in prostate stromal cells. Conclusion: These results suggest that in prostate stromal cells, progesterone decreases androgen receptor protein expression, which results in decrement of COX-2 protein expression. This effect might be mediated by post-transcriptional regulation.

[P2-27] The Monitoring of Methylmercury in Abyssal Fish Species by GC-ECD

Seong-Cheol Kim,Sung-Im Im,Kwang-Soo Lee,Hyun-A Kim,Kyung-A An,Jin-Wook Jang,Ryun-Kyung Lee,Mi-Ok Kim,Jeong-Min Kim,Yong-Seok Ko,Dae-Byoung Kim,So-Hee Kim 한국식품영양과학회 2007 한국식품영양과학회 학술대회발표집 Vol.2007 No.10

벼줄무늬잎마름바이러스의 대 발생과 발생 요인

김정수(Jeong Soo Kim),이관석(Gwan Seok Lee),김창석(Chang Seok Kim),최홍수(Hong Soo Choi),이수헌(Soo Heon Lee),김미경(Mi Kyeong Kim),곽해련(Hae Ryun Kwag),남문(Mun Nam),김정선(Jeong Sun Kim),노태환(Tae Hwan Noh),강미형(Mi Hyung Kang) 한국농약과학회 2011 농약과학회지 Vol.15 No.4

Clinical Features of Waldenstrom Macroglobulinemia in Korea

( Soo Mee Bang ),( Sook Ryun Park ),( Se Hoon Park ),( Eun Kyung Cho ),( Sung Soo Yoon ),( Dong Bok Shin ),( Jae Hoon Lee ),( Seon Yang Park ),( Byoung Kook Kim ),( Noe Kyeong Kim ) 대한내과학회 2004 The Korean Journal of Internal Medicine Vol.19 No.3

Virus Incidence of Sweet Potato in Korea from 2011 to 2014

Kim, Jaedeok,Yang, Jung wook,Kwak, Hae-Ryun,Kim, Mi-Kyeong,Seo, Jang-Kyun,Chung, Mi-Nam,Lee, Hyeong-un,Lee, Kyeong-Bo,Nam, Sang Sik,Kim, Chang-Seok,Lee, Gwan-Seok,Kim, Jeong-Soo,Lee, Sukchan,Choi, Hon The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.5

A nationwide survey was performed to investigate the current incidence of viral diseases in Korean sweet potatoes for germplasm and growing fields from 2011 to 2014. A total of 83.8% of the germplasm in Korea was infected with viruses in 2011. Commercial cultivars that were used to supply growing fields were infected at a rate of 62.1% in 2012. Among surveyed viruses, the incidence of five Potyvirus species that infect sweet potato decreased between 2012 and 2013, and then increased again in 2014. Representatively, the incidence of Sweet potato feathery mottle virus (SPFMV) was 87.0% in 2012, 20.7% in 2013 and then increased to 35.3% in 2014. Unlike RNA viruses, DNA viruses were shown to decrease continuously. The incidence of Sweet potato leaf curl virus (SPLCV) was 5.5% in 2003, 59.5% in 2011, and 47.4% in 2012. It then decreased continuously year by year to 33.2% in 2013, and then 25.6% in 2014. While the infection rate of each virus species showed a tendency to decline, the virus infection status was more variable in 2013 and 2014. Nevertheless, the high rate of single infections and mixed infection combinations were more variable than the survey results from 2012. As shown in the results from 2013, the most prevalent virus infection was a single infection at 27.6%, with the highest rate of infection belonging to sweet potato symptomless virus-1 (SPSMV-1) (12.9%). Compared to 2013, infection combinations were more varied in 2014, with a total of 122 kinds of mixed infection.

First Report of Cucumber mosaic virus Isolated from Wild Vigna angularis var. nipponensis in Korea

Kim, Mi-Kyeong,Jeong, Rae-Dong,Kwak, Hae-Ryun,Lee, Su-Heon,Kim, Jeong-Soo,Kim, Kook-Hyung,Cha, Byeongjin,Choi, Hong-Soo The Korean Society of Plant Pathology 2014 Plant Pathology Journal Vol.30 No.2

A viral disease causing severe mosaic, necrotic, and yellow symptoms on Vigna angularis var. nipponensis was prevalent around Suwon area in Korea. The causal virus was characterized as Cucumber mosaic virus (CMV) on the basis of biological and nucleotide sequence properties of RNAs 1, 2 and 3 and named as CMV-wVa. CMV-wVa isolate caused mosaic symptoms on indicator plants, Nicotiana tabacum cv. Xanthi-nc, Petunia hybrida, and Cucumis sativus. Strikingly, CMV-wVa induced severe mosaic and malformation on Cucurbita pepo, and Solanum lycopersicum. Moreover, it caused necrotic or mosaic symptoms on V. angularis and V. radiate of Fabaceae. Symptoms of necrotic local or pin point were observed on inoculated leaves of V. unguiculata, Vicia fava, Pisum sativum and Phaseolus vulgaris. However, CMV-wVa isolate failed to infect in Glycine max cvs. 'Sorok', 'Sodam' and 'Somyeong'. To assess genetic variation between CMV-wVa and the other known CMV isolates, phylogenetic analysis using 16 complete nucleotide sequences of CMV RNA1, RNA2, and RNA3 including CMV-wVa was performed. CMV-wVa was more closely related to CMV isolates belonging to CMV subgroup I showing about 85.1-100% nucleotide sequences identity to those of subgroup I isolates. This is the first report of CMV as the causal virus infecting wild Vigna angularis var. nipponensis in Korea.

Development of Parameters for Diagnosing Laryngeal Diseases

Kim, Yong Ju,Wang, Soo Geun,Kim, Gi Ryun,Kwon, Soon Bok,Jeon, Kye Rok,Back, Moo Jin,Yang, Byung Gon,Jo, Cheol Woo,Kim, Hyung Soon 한국음성과학회 2003 음성과학 Vol.10 No.1

Many people suffer from various laryngeal diseases. Since we can notice voice change easily, acoustic analysis can be helpful to diagnose the diseases. Several attempts have been made to clarify the relation between the parameters and the state of ssick vocal folds but any decisive parameters are not found yet. The purpose of this study was to select and develop those parameters useful for diagnosing and differentiating laryngeal diseases. We examined eight MDVP parameters, and two additional MFCC and LPC parameters obt ained from the production of an open vowel by 252 subjects with or without laryngeal diseases. Using a statistical procedure through the artificial neural networks, we attempted to differentiate laryngeal disease groups. Results showed that the LPC parameters indicated the highest differentiating rate by the networks followed by the MFCC and the MDVP parameters. In addition, Jita, Shim and NHR among the MDVP parameters came out better parameters in diagnosing laryngeal diseases.

Distinct clinical features and outcomes in never‐smokers with nonsmall cell lung cancer who harbor <i>EGFR</i> or <i>KRAS</i> mutations or <i>ALK</i> rearrangement

Kim, Hye Ryun,Shim, Hyo Sup,Chung, Jin‐,Haeng,Lee, Young Joo,Hong, Yun Kyoung,Rha, Sun Young,Kim, Se Hoon,Ha, Sang‐,Jun,Kim, Se Kyu,Chung, Kyung Young,Soo, Ross,Kim, Joo Hang,Cho, Byoung C Wiley Subscription Services, Inc., A Wiley Company 2012 Cancer Vol.118 No.3

<P><B>Abstract</B></P><P><B>BACKGROUND:</B></P><P>The objectives of this study were to determine the proportions of major oncogenic alterations and to examine survival in genotype‐specific subsets of never‐smokers with nonsmall cell lung cancer (NSCLC).</P><P><B>METHODS:</B></P><P>The authors concurrently analyzed mutations in the epidermal growth factor receptor (<I>EGFR</I>) and v‐Ki‐<I>ras</I>2 Kirsten rat sarcoma viral oncogene homolog (<I>KRAS</I>) genes and investigated anaplastic lymphoma kinase (<I>ALK</I>) gene rearrangements in samples from 229 never‐smokers with NSCLC. <I>ALK</I> rearrangements were identified by fluorescent in situ hybridization and were confirmed by immunohistochemistry. Mutations in <I>EGFR</I> (exons 18 to 21) and <I>KRAS</I> (codons 12 and 13) were determined by direct sequencing.</P><P><B>RESULTS:</B></P><P>Of 229 tumors, the frequency of <I>EGFR</I> mutations, <I>ALK</I> rearrangements, <I>KRAS</I> mutations, and no mutations (wild type [WT]) in any of the 3 genes (<I>WT/WT/WT</I>) was 48%, 8.3%, 3.5%, and 40.2%, respectively. All genetic alterations were mutually exclusive. The median progression‐free survival after treatment with EGFR tyrosine kinase inhibitors (TKIs) was 12.8 months, 6.3 months, 2.1 months, and 1.6 months in patients with <I>EGFR</I> mutations, the <I>WT/WT/WT</I> genotype, <I>KRAS</I> mutations, and <I>ALK</I> rearrangements, respectively. In a Cox regression model, the adjusted hazard ratio for the risk of disease progression after treatment with EGFR TKIs was 0.59 (95% confidence interval [CI], 0.40‐0.87; <I>P</I> = .008) for patients with <I>EGFR</I> mutations, 4.58 (95% CI, 2.07‐10.15; <I>P</I> < .001) for patients with <I>ALK</I> rearrangements, and 4.23 (95% CI, 1.65‐10.8; <I>P</I> = .003) for patients with <I>KRAS</I> mutations. Overall survival also differed significantly among genotypes.</P><P><B>CONCLUSIONS:</B></P><P>To the authors' knowledge, this was the largest comprehensive and concurrent analysis to date of 3 major oncogenic alterations in a cohort of East Asian never‐smokers with NSCLC. Because survival outcomes differed among genotypes, and drugs that target specific alterations currently are available, genetic profiling to identify genotype‐specific subsets can lead to successful treatment with appropriate kinase inhibitors. Cancer 2012;. © 2011 American Cancer Society.</P>