HCV, Alcoholic : PE-096 ; Clinical and histopathological features of acute onset autoimmune hepatitis

( Min Young Rim ),( Soo Yong Park ),( In Ku Yo ),( Min Su Ha ),( Ju Seung Kim ),( Ju Won Lee ),( Young Kul Jung ),( Dong Hae Chung ),( Oh Sang Kwon ),( Yun Soo Kim ),( Duck Joo Choi ),( Ju Hyun Kim1 ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: Autoimmune hepatitis (AIH) is a progressive inflammatory liver disease of unknown etiology that is reported to be a consequence of aberrant autoreactivity. Several studies which reported the acute presentation of AIH have different clinical course and histologic features. In this study, we compared acute presentation of AIH and chronic presentation of AIH. Methods: We retrospectively reviewed the medical records of patients with autoimmune hepatitis from January 2003 to June 2011 at Gachon University, Gil Hospital. A total of 29 patients were enrolled, 7 patients were diagnosed with acute presentation of AIH. Results: There was no difference between two group in age, gender, and score system of AIH. Patients with acute presentation had higher serum levels of total bilirubin, lower serum levels of albumin in clinical feature (p<0.05), and higher frequency of zone 3 necrosis in histologic feature. The cumulative incidental rate of the normalization of serum AST and ALT levels with prednisolone treatment was similar between patients with acute presentation and chronic presentation in clinical course. Conclusions: Higher AST, ALT and, bilirubin were clinical specific feature, and zone 3 necrosis is a histological characteristic of autoimmune hepatitis with acute presentation.

嫌氣性 消化에 미치는 黃酸鹽의 影響

林哉明,全基一,金正權,金守明 동아대학교 공과대학 부설 한국자원개발연구소 1988 硏究報告 Vol.12 No.2

The competition for substrate between sulfate reducing bacteria (SRB) and methane producing bacteria (MPB), and the inhibitory effects of sulfide produced from microbial sulfate reduction on sulfate reducers and methanogens were investigated in this study by using laboratory digesters. The main results of this experimental study are as follows ; 1) When SRB and MPB was co-cultured, the microbial sulfate reduction occurred first, so that the inhibition of volatile acid on MPB were eliminated. 2) When SRB and MPB competed each other for substrate, the shorter the sludge retention time (SRT), the more dominate species the SRB were. 3) The limit SRT for MPB was above 4.5days, and that for SRB was near 2days. 4) When the carbon source (COD) loading was kept at the same level in comparison with different SO₄^(2-) Loading, biogas production was not inhibited when the ratio of COD to SO₄^(2-) Was above 2.7, that was begun to be inhibited when the ratio was under 2.7, and that was not occurred when the ratio was under 1.0. 5) When the sulfate ion loading was kept at the same level with different carbon source (COD) loading, the biogas production was begun to be retarded when the ratio of COD to SO₄^(2-) Was under 1.0, and that was not occurred when the ratio was 0.5. 6) Finally, when influent concenration of SO₄^(2-) was 1200㎎/l, or sulfide concentration in the digester was above 130㎎/l, the biogas production was begun to be retared. As the influent concentration of SO₄^(2-) was above 2000㎎/l, or the concentration of sulfide in the digester was near 180㎎/l, the biogas production was completely inhibited.

白鼠感染 肝吸蟲幼蟲에 대한 Praziquantel(Biltricide^(�))의 殺蟲效果에 대하여

林漢鍾,梁元容,宋寅喆,金洙鎭 최신의학사 1981 最新醫學 Vol.24 No.4

濟州 漢拏山에서의 垂直高度에 따른 蘭草科(Fam. Orchidaceae) 植物의 分布와 生態

金秀南,林暎得 仁川敎育大學校 1994 論文集 Vol.28 No.1

Chejudo is richest in orchid plants in Korea. Orchids of Chejudo consist of four tribes, 35 genera and 64 species. Shadow-tolerant type is 29 species and others are 11 species. The zone of broad-leaved evergreen forest has 55 species, needdle leaved forest zone 18, shrub zone 9, grasslands 8, and alpine forest four respectively.

活性 슬러지에 의한 有害 廢水 處理度에 관한 硏究 : Formaldehyde 廢水 Formaldehyde Wastewater

林哉明,全基一,金守明 동아대학교 환경문제연구소 1990 硏究報告 Vol.13 No.1

The purpose of this research was to examine the possibility of biodegradation of the hazardous wastewater -formaldehyde wastewater- and to determine the engineering design parameters by the activated sludge process. The main results of this experimental study are as follows: 1) It was indicated that formaldehyde wastewater can be treated by Activated sludge, and to accmplish the COD removal efficiency 90%, the HRT was above 11 days, COD volume loaling was below 096㎏ COD/㎥·d, and F/M_(v) ratio would be expected to have below 0.45 2) Oxygen reguirment was above 0.81㎏ O₂/㎏ CODrm and Sludge production was about 0.1㎏ VSS/㎏ CODrm.

요꼬가와吸蟲에 대한 Albendazole의 영향에 관한 電子顯微鏡的 硏究

金永洙,金洙鎭,林漢鍾 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.1

Newly developed albenzole(Zentel® is a broad spectrum anthelmintic with high activity against intestinal nematodes as well as trematodes and cestodes infections. The present study was undertaken to observe the morphological changes of Metagonimus pokogcwa; which was obtained from experimentally infected dogs after treatment with Albendazole. For this study 5 dogs were artificially infected with metacercariae isolated from Plecoglossus altivelist and 3 of them were given albendazole 2 doses of 100mg at the day of 4 weeks after infection. The Motagonimus worms were collected from the small intestine of the dogs which : were autopsied at 24hours after treatment. The fine structures of the collected worms were studied by means of scanning transmission electron microscopy. The findings of the observation were compared with those of untreated worms. The results are as follows: 1. It scanning electron microscopy(SEM), the worms obtained from non-treated dogs showed that the whole surface was armed with many scale-like spices, however the worms obtained from the treated dogs showed that many partially necrotized regions on the tegument were scattered elsewhere surface of the worms. 2. In transmission electron microscopy(TEM), numerous mitochondriae and high electron dense granules were distributed in the tegumental syncytium and muscle layers in the control normal worms. However, in the worms obtained from the treated dogs, the degeneration of mitochondriae and disappearance of high electron dense granules were observed in the tegumental syncytium and muscular layers. It was also observed that some tegumental syncytium and circular muscle layers were detached from basement membrane 3. By Sem observation, the bleb-like structures were formed on the surface of the damaged tegument but not on the sensory papillae of the worms obtained from the treated dogs, and the cilium protruded from the dilated sensory papillae. 4. By TEM observation, it was observed that the degeneration of vesicles in the nerve bulb of the sensory papillae in formed vacuoles in its sensory papillae in the worms obtained from the treated dogs.

Praziquantel이 肝吸蟲에 미치는 영향에 관한 電子顯微鏡的 硏究

金相淳,金洙鎭,林漢鍾 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.1

A study was undertaken to observe the morphological changeds of Clonorchis sinensis which was obtained from experimentally infected rats after treatment with praziquantel a newly developed anthelmintic agent. For this study the rats were artificially infected with metacercariae isolated from Pseudorasbora parva and were given praziquantel a single dose of 600 ㎎ per kg of body weight at 5 weeks after infection. The Clonorchis worms were collected from the bile ducts of the which were autopsied at 6, 12 and 24 hours after treatment. With the collected worms, the fine structure was studied by means of scanning and transmission electron microscope. The findings of the structure was studied by means of scanning and transmission electron microscope. The findings of the observation were compared with those of untreated worms. The results are as follows: 1. At six hours after treatment with praziquantel, the degeneration of mitochondia and vesicles in the nerve bulb of the sensory papillae around two suckers and excretory pore was observed. 2. At twelve hours after treatment, degenerated changes on the tegument of C. sinensis were occurred by a considerable vacuolizatioon of the tegumental syncytium near the sensory papillae. 3. Twenty-four hours after treatment, the vacuolization had increased markedly in the tegumental syncytium and the vacuoles appeared in the tegumental ridge. The vacuoles finally ruptured on the tegumental surface and formed bleb-like structures. In course of time the bleb-like structures were increased in number all over the surface of the worm. 4. The balloon-shape structure formed at anterior part of worm from 12 hours after treatment is supposed to be caused by the accumulated excretory material due to the loss of excretory function. Therefore the bleb-like structures and a balloon-shape structure on the surface of worms led to its death by the bursting of the tegument in this region.

淨化槽SLUDGE의 堆肥化

林哉明,金秀生,李賢珠 동아대학교 환경문제연구소 1986 硏究報告 Vol.9 No.1

This study was performed for composting of septic tank sludge by batch type enclosed composter. The conditions of each element were the mixed rate 0.5:1∼4:1 of septic tank sludge rice-polishing, initial Moisture Content rate of 50 to 70%, C/N ratio of 8 to 43, and 0.25∼1.51/min·㎏ per volatile solids of Air Quantity. The results of this experiment are as follows: (1) The condition of the most successful composting was mixed rate 2.1 of septic tank sludge and rice-polishings in each sample. (2) The range of the optimum moisture content was 60∼65% simultaneously, mixed rate was 2:1-3:1 the temperature of compost pile for hygienic stabilization was sustained for minimum seven days. (3) The range of optimum C/N ratio 30∼35 showed the fastest and most successful composting . (4) 0.25 l/min-㎏ VS Air Quantity was the optimum of composting. (5) In a day or two, the maximum temperature of 48∼54℃ showed that after inputing composter under the adequate condition of each element: Moisture Content, Air Quantity, pH, C/N ratio etc. The maximum CO₂ production quantity with temperature variation was 8∼11% at 44∼50℃.

間接螢光抗體法 및 免疫黃金標識法을 이용한 肝吸蟲組織內 抗原性部位에 관한 硏究

成大林,金洙鎭,嚴基善,林漢鍾 고려대학교 의과대학 1988 고려대 의대 잡지 Vol.25 No.3

Indirect fluorescent antibody test and immunogold labeling method were accomplished to observe the antigenic localization in the tissues of adult Clonorchis sinesis. Immunoglobulins (IgG) for the reaction were obtained from white rats and rabbits, at eight weeks after experimental infection with 50 and 500 metacercariae of C. sinensis, respectively. Frozen sectioned adult worm antigen (8μm) and FITC-conjugated goat anti-rat, rabbit IgG were used for indirect fluorescent antibody test. On the other hand, sectioned tissue antigen embedded in Lowcryl HM 20 medium and secondary antibody protein A gold complex (particle size:12 nm) were used for immunogold labeling method. In the results, fluorescence or gold particle were not observed on the tissue antigens of the worm when reacted with the noninfected control IgG. However, with the infected animal groups IgG, fluorescence or gold particles were observed on the interstitial matrix (body fluid) between the various organs, such as the tegumental cells, parenchyma, testes, receptaculum seminis. And the cecal epithelium including cecal contents and epithelium of excretory bladder also showed strong reaction. Therefore, antigenic materials which stimulate the IgG antibodies against the host were thought to be fluid substances and digestive enzymes or excretory materials which make up the interstitial matrix in the tissues of the worm.

矮小條蟲(Hymenolepis nana)에 대한 Albendazole의 영향에 관한 電子顯微鏡的 硏究

王辰聲,金洙鎭,林漢鍾 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.3

Newly developed albendazole (Zentel®) is a broad spectrum anthelmintic with high activity against intestinal nematodes as well as trematodes and cestodes infections. The present study was undertaken to observe the morphological changes of Hymenolepis nana which obtained from experimentally infected mice after treatment with albendazole. For this study 20 mice were artificially infected with eggs and 16 of them were given albendazole (1×100mg/kg) four weeks after infection. The worms were collected from the small intestine of the mice which were autopsied at 1 and 2 hours after treatment. The fine structure of the collected worms were studied by means of scanning and transmission electron microscopies. The findings were compared with those of untreated worms. The results are as follows: 1. The worms obtained from non-treated mice showed that the whole surface is covered with many microtriches. Microtriches of H. nana may be divided into three anatomical regions; a base, junctional region and cap. Tegumental layer showed well developed syncytium which included a lot of number of discoidal secretory bodies. 2. Many blebs were formed on the surface of the damaged tegument of the worm at 1 hour after treatment with albendazole. The bleb was packed by plasma membrance and included matrix which uniform electron density. Some bleb surface has microtriches. 3. The destruction of tegumental syncytium was recognized at 2 hours after treatment with albendazole. Therefore the blebs on the worm sufrace caused to the death by the tegumatal bursing.