Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

전기이중층 캐패시터의 집전체에 자성물질의 도금과 전기화학적 특성

최진권, 박수길 충북대학교 건설기술연구소 2013 충북대학교 건설기술연구소 논문집 Vol.32 No.1

본문참고

아미노산용액에서 팔라듐의 전기화학적 행동

권영순,이수진 가톨릭대학교 자연과학연구소 1998 자연과학논문집 Vol.19 No.-

팔라듐을 0.01M proline 용액에서 펄스 차이 폴라로그래피법으로 정량하였다. 검출 한계는 4.5×10^(-8)M Pd(Ⅱ)이고 봉우리 전류가 선형인 농도 범위는 6.0×10^(-8)~2.0×10^(-7)M Pd(Ⅱ)이었다. 촉매 수소 전류가 생성되는 메카니즘은 chemisorption이 일어나는 BC과정임을 알 수 있었다. The measurement of trace palladium in 0.01M proline solution by differential pulse polarographic method is described. In this medium the detection limit and the dynamic range are 4.5×10^(-8)M and 6.0×10^(-8)~2.0×10^(-7)M for palladium. The formation mechanism of catalytic hydrogen wave is BC process coupled with chemisorption.

여학생 동복 하의 착용시의 생리반응과 주관적 착용감

권수애,오미경,강미영,송혜원,박진채,권리현 충북대학교 교육 ·생활연구소 생활과학연구센터 2002 생활과학연구논총 Vol.5 No.-

In this study, physiological responses and subjective sensations were investigated depending on various high school girls' lower cloth uniform in winter. Four healthy young women were selected, and they wore same underwear and jacket. The types of lower cloth were tight skirt+stocking, flared skirt+stocking, tight skirt+legings+socks, flared skirt+legings+socks, and slacks+socks. The results were as follows: 1) The effect of the type of lower cloth in winter on the skin temperature, humidity inside clothing and subjective sensations were significant. 2) It is desirable for high school girls to wear slacks in winter, Since physiological responses and subjective sensations of slacks are superior to those of skirts. 3) It is desirable for high school girls to wear flared skirt, legings, socks in winter, Since insulation and subjective sensations of flared skirts are superior to those of tight skirts.

연상단어 순위로 본 정신질환자의 죽음의식에 관한 연구

권혜진,이소우 중앙대학교 의과대학 의과학연구소 1983 中央醫大誌 Vol.8 No.1

The purpose of this study was to explore the death consciousness of 60 psychiatric patients hospitalized in the 3 general hospitals with similar therapeutic environments in Seoul by means of free word association method. 10 words identified to be most frequently associated with death in the Kwon's study in 1980 were used in this study. 5 hypotheses were formed to achieve the objectives of the study. It's major findings are an follows; 1. In this study suicide topped the list of the 10 words followed by 'Disease', 'Graves', 'Emptiness', 'Incurable Disease', Frustration', 'Death', 'Disaster', 'Grief', and 'Darkness' in the responses of the psychiatric patients. The first hypothesis was found statistically significant and therefore, was verified. 2. No difference statistically significant was found in the order of frequencies in the patients' association of words related to 'death' by sex or age. 3. However, there was statistically significant difference in the order of frequencies in the patients' association of words by dignosis. Schizophrenic patients associated 'death' most frequently with emptiness and neurotic patients with 'incurable disease' while in the case of psychoneurotic patients, 'suicide' topped the list. Therefore the fourth hypothesis was verified. 4. There was a statistically significant difference by behavior characteristics, and the fifth hypothesis was denied.

EDTA와 Nd:YAG laser가 근관충전 후 치근단 미세누출에 미치는 영향

권진수,이희주,허복 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.1

The purpose of this study was to evaluate the effects of EDTA and pulsed Nd:YAG laser on apical leakage of canal obturation. Forty-eight single-rooted teeth were used in this study. The teeth were instrumented up to a size 40 K-file and irrigated with 2.5% NaOCI between each file size. And the teeth were divided into 4 groups. In group A. the root canals were irrigated with a final flush of 5ml 17% EDTA. The teeth in group C and D were irradiated by pulsed Nd:YAG laser(laser parameters were set at 1W, 100mJ, 10Hz, and 2W, 100mJ, 20Hz respectively). The results were as follows; 1. Apical leakage was observed in 50% of samples in group A, 30% of samples in group B, 20% of samples in group C, and 10% of samples in group D. 2. The teeth in group B had less leakage than group A, but there was no statistically significant differences(p>0.05). 3. The teeth in group C, D had less leakage than group A, and there was statistically significant differences(p<0.05). 4. The teeth in group C, D had less leakage than group B, but there was no statistically significant differences(p>0.05). 5. There was no significant differences in apical leakage between group C and group D(p>0.05).

임상검체에서 분리된 그람 음성 세균들에 대한 Isepamicin의 시험관내 항균력

권현희,박수진,소민욱,박현구,최성호,김미나,최상호,정진용,우준희,김양수 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background : The increasing incidence of multidrug-resistant gram-negative bacteria causing nosocomial infections is an important clinical problem. Isepamicin is a recently developed aminoglycoside which has been known to have potent activity against gram-negative organisms. We evaluated the in vitro activities of isepamicin and other aminoglycosides against a large number of gram-negative organisms. Materials and Methods : We tested the in vitro antimicrobial activities of isepamicin, amikacin, gentamicin, and tobramycin against 566 gram-negative organisms collected between January 2006 and June 2006 in Asan Medical Center. Minimal inhibitory concentrations (MICs) were determined and interpreted according to the recommendations of Clinical and Laboratory Standard Institute (CLSI). The breakpoint MIC used for interpretation of isepamicin was MIC≤16 ㎍/mL as susceptible, 32 ㎍/mL as intermediate, and 64 ㎍/mL as resistant. Results : The MIC_(50)/MIC_(90) of isepamicln for Escherichia coli, Klebsiella pneumon/ae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Enterobacter cloacae were 1/2, 0.5/>128, 4/16, 16/>128, and 1/2 ㎍/mL, respectively. The susceptibilities for E. coli, K. pneumoniae, P. aeruginosa, A. baumannii, and E. cloacae were 100%, 86.4%, 89.7%, 50.0%, and 96.6%, respectively. For E. coli, K. pneumoniae, P. aeruginosa, and E. cloacae, isepamicln had better in vitro activities than gentamicin and tobromycin, and had similar activities with amlkacin. For A. baumanii, all four tested aminoglycosides had similar in vitro activities. Conclusion : Isepamicin had excellent in vitro activities against gram-negative organisms, except A. baumanii. The overall in vitro activities were similar with amikacin. 목적 : 그람 음성 세균은 다양한 지역사회 및 병원 획득 감염의 원인균이다. 최근에는 병원 감염을 일으키는 그람 음성 세균에서 항균제 내성균의 출현과 확산이 큰 문제가 되고 있다. Isepamicin은 최근에 소개된 gentamicin B의 유도체로 외국에서의 연구에 의하면 그람 음성 세균들에 대한 항균력이 우수함이 보고 된 바 있다. 본 연구에서는 우리나라의 임상 균주들을 대상으로 그람 음성세균에서 isepamicin의 시험관내 항균력을 기존의 아미노배당체 제재들과 비교하여 평가하고자 하였다. 재료 및 방법 : 2006년 1월에서 6월 사이에 서울아산병원 환자에서 분리된 Escherichia coli 113 균주, Klebsiella pneumoniae 110 균주, Pseudomonas aeruginosa 117 균주, Acinetobacter baumannii 108 균주, 그리고 Enterobacter cloacae 118 균주를 대상으로 isepaimcin, amikacin, gentamicin, tobramycin에 대한 최소억제농도(minimal inhibitory concentration, MIC)를 한천 희석법(Mueller-Hinton Agar)을 이용하여 측정하였다. 감수성여부는 CLSI 기준에 따랐으며, isepamicin은 MIC≤16㎍/mL를 감수성, MIC=32 ㎍/mL를 중등도 내성, MIC≥64 ㎍/mL를 내성으로 판정하였다. 결과 : Isepamicm의 E. coli, K. pneumoniae, P. aeruginosa, A baumannii, E. cloacae에 대한 MIC_(50)/MIC_(90)은 각각 1/2, 0.5/>128, 4/16, 16/>128, 1/2 ㎍/mL였고, 항균제 감수성률은 차례대로 100%, 86.4%, 89.7%, 50.0%, 96.6%였다. 기존 아미노배당체 제재들과 비교하면 E. coli, K. pneumoniae, P. aeruginosa, E. cloacae에서는 amikacin과 비슷한 감수성률을 보였고, gentamicin과 tobramycin보다 높은 감수성률을 보였다. A baumannii의 경우 isepamicin의 감수성률이 50.0%로 비교적 낮았지만 amikacin, gentamicin, tobramycin도 각각 50.0%, 46.3%, 47.2%의 감수성률을 나타내어 4가지 항균제가 모두 비슷한 항균력을 보였다. 결론 : Isepamicin은 그람 음성 세균들에 있어 기존의 아미노배당체 제재들과 비교했을 때 amikacin과는 동등한 시험관내 항균력을 보이고 gentamicin, tobramycin보다 뛰어난 시험관내 항균력을 보여 사용이 유망한 아미노배당체 중 하나라 생각된다.

압전 비틀림 파동 변환기 제작 및 평가

권오수,김진오 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.4

봉에서 전파하는 비틀림파를 발생시키는 압전 비틀림 파동 변환기를 제작하였다. 비틀림 파동을 발생시키는 원리는 원주방향으로 분극된 압전 원판에 인가된 두께 방향 전압에 의해 나타나는 원주방향 전단운동이다. 압전 비틀림 원판을 제작한 후, 공진 주파수를 낮추기 위하여 랑주방(Langevin)형 비틀림 변환기를 제작하였다. 제작된 변환기들의 공진 주파수를 측정한 결과, 해석에 의해 예상된 특성과 잘 일치하였다. Piezoelectric torsional transducers have been fabricated to generate waves in a rod. The principle of generating torsional wave is based on the use of the circumferential shear motion derived by axial voltage exerted on the piezoelectric disk poled in the circumferential direction. The natural frequency of the piezoelectric torsional disk has been reduced by fabricating Langevin-type torsional transducers. The natural frequencies of the fabricated transducers have been measured and have shown good agreement with the characteristics predicted by analysis.

主觀的인 評價에 의한 靑少年의 肥滿傾向 分析

權善鈺,金基學,李東洙,鄭鎭國 慶北大學校 師範大學 體育學硏究會 1992 體育學會誌 Vol.20 No.-

This study was conducted to analyze the tendency of obesity by subjective evaluation method in measuring the skinfold thickness in adolescents. The validity norms were height, weight, the sum of skinfold thickness, Db1, %Fat1, %Fat2, and BMI. The subjects surveyed were 2,442 boy and girl students of middle and high school in medium and small city and big city. The results are summarized as follows; 1. Weight of adolescents evaluated as obesity was over 76㎏ in boy's middle, over 67㎏ in gril's middle school, over 79㎏ in boy's high school, and over 68㎏ in girl's high school, the sum of skinfold thickness was over 40㎜, over 39㎜, over 54㎜, and over 41㎜ respectively. 2. Except the case of height in girl's middle, boy's high and girl's high school, in height in boy's middle, weight, the skinfold thickness, Db1, %Fat1, %Fat2, and BMI, difference among the degree of obesity evaluated subjectively was significant(p<.05). 3. Except heigh in girl's middle, boy's and girl's high school, correlation between the degree of obesity evaluated subjectively and height in girl's middle school, weight, the sum of skinfold thickness, Db1, %Fat1, %Fat2, BMI respectively was high and significant(p<.01 or p<.001) Therefore, on the basis of above results, I think we can evaluate the tendency of obesity in adolescents validly by subjective evaluation method as well.

세기변조방사선치료의 선량분포 검증에 관한 연구

권수일,신동호,송재영,안진호 京機大學校 2006 論文集 Vol.50 No.-

Film dosimetry as a part of patient specific intensity modulated radiation therapy quality assurance (IMRT QA) has been accomplished to develop the new optimization method of film isocenter offset and to suggest the new quantitative criteria for film doimetry after optimization. Five patients with head and neck cancers were measured in IMRT dosimetry by film method. Optimization method with the local minimum was developed to adjust the film isocenter offset error, which is the biggest part of systemic errors. The mean ratios were 5.33% and 7.09% for 5mm and 1 mm resolution dose data optimization respectively.