림프절외 NK/T세포 림프종에서 Epstein-Barr Virus에 대한 연구

조민선,우소연 이화여자대학교 의과대학 2004 EMJ (Ewha medical journal) Vol.27 No.2

목적 : NK/T 세포 림프종의 발생은 EBV와 연관되어 있으며, EBV 아형과 LMP-1유전자의 30염기쌍 결손 돌연변이가 EBV의 종양유발성과 관련 있다는 연구 결과가 있는 바, 본원에서 NK/T 세포 림프종으로 진단된 예를 대상으로 이에 대해 알아보고자 하였다. 대상과 방법 : 본원에서 진단된 NK/T세포 림프종 16예를 대상으로 EBER-1 in situ hybridization으로 EBV 양성율을 검사하고, PCR방법을 통해 EBV아형과 결손 돌연변이 유무를 관찰하였다. 결과 : 16예 모두 EBER-1 in situ hybridization에서 양성반응을 보였다. EBV는 모두 A 아형이었고 LMP-1의 30염기쌍 결손은 전체 16예 중 10예(62.5%)에서 있었고 나머지 6예는 wild type이었다. 결론 : 본원에서 진단된 NK/T세포 림프종 모든 예어서 EBV를 발견할 수 있었으며, EBV는 모두 A아형이었다. LMP-1 결손 돌연변이는 62.5%에서 관찰되었으며 예후와의 연관성은 보다 많은 예에 대한 연구를 통해 밝혀져야 할 것이다. Objectives: Epstein-Barr virus(EBV) is associated with development of various types of lymphoma, especially NK/T cell lymphoma. Recently, its subtypes and LMP-1, major oncoprotein of EBV, have been studied. We investigated the frequency of EBV, its subtypes, and LMP-1 status on the cases diagnosed at Ewha university hospital between 1993 and 2002. Material and Methods: Sixteen cases of NK/T cell lymphomas were studied. In situ hybridization for EBER-1 mRNA and PCR for EBV subtypes and 30 base pair deletion of LMP-1 were done. Results: All cases showed EBV positivity by EBER in situ hybridization. All cases contained Type A viruses and 10 cases(62.5%) revealed LMP-1 30bp deletion. Conclusion: EBV act as a causative role in the development of NK/T cell lymphoma. The exact role of LMP-1 30bp deletion variant in the lymphomatogenesis should be studied with larger number of cases.

T Cell Receptor Rearrangement in NKT Cells Differentiated from Cord Blood CD34+ Cells

정윤재,우소연 이화여자대학교 의과대학 2003 EMJ (Ewha medical journal) Vol.26 No.2

Objectives: To investigate the time of rearrangement of the TCR gene in the process of NKT cell differentiation from CD34+ human cord blood cells in vitro. Methods : We isolated the CD34+ human cord blood cells and induced the differentiation of NKT cells by liquid culture including IL-15, FL and SCF for 30 days. In order to detect the time of TCR gene rearrangement in differen-tiated NKT cell, we performed PCR for TCR-rearrange-ment excision circles (TRECs) with isolated DNA. Results : Signal joint TREC first appeared on day 4 or day 8 and continuously existed until day 30. Between day 9 and day21, cells showed high output of coding joint TRECs after signal joint rearrangement. Conclusion : In differentiated NKT cells, TCR gene rearrangement started within a week after culture started and mostly occurred in 2 to 3 weeks after culture started. 목적: 제대혈에서 분리한 CD34 양성세포를 사이토카인을 이용하여 NKT 세포로 분화시키는 과정에서 T 림프구의 수용체(TCR) 유전자의 재배열이 어느 시기에 나타나는지 알아보고자 하였다. 방법: 제대혈에서 분리한 CD34 양성세포에 IL-15, Flt3-L와 stem cell factor를 첨가하여 액체 배양하였다. 일주일 간격으로 세포를 수거하여 DNA를 분리하고, 이것을 TCR 유전자 재조합의 결과로 생겨나는 TCR-rearrangement excision circles (TRECs)을 검출하는 시발체(primer)를 사용하여 중합효소연쇄반응을 시행하였다. 결과: 배양 시작 4일째부터 TREC이 검출되어 배양 후 30일까지 검출되었으나, 배양 시작 2~3주에 그 정도가 가장 높았다. 결론: 제대혈에서 분리한 CD34 양성세포에서 분화하는 NKT 세포의 TCR 유전자 재조합은 배양 개시 후 일주일 이내에 시작되어 배양 개시후 2~3주에 활발히 일어난다.

메조포어 물질을 이용한 TiO_2의 제조와 광촉매 산화반응에의 적용

이한수,윤봉국,우창수,김소영,He, NongYue,이호인 한국공업화학회 2003 응용화학 Vol.7 No.1

TiO_2-loaded mesoporous materials were prepared by sol-gel method. Mesoporous materials suppressed the phase transformation of TiO_2 from anatase to rutile and increased the bandgap of TiO_2 which was explained in terms of the quantum size effect. Several kinds of nano-sized TiO_2 with particle sizes of 5∼20 nm, were obtained through the removal of the mesoporous materials by etching in 2 M NaOH or the calcination under air condition. They showed good activites in the photocatalytic oxidation of 2-isopropyl-6-methyl-4-pyrimidinol.

정중운동신경과 척골운동신경의 전기생리학적 연구

김종순,이현옥,안소윤,구봉오,남건우,김영직,김호봉,류재관,류재문 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.2

The determination of peripheral nerve conduction velocity is and important part to electrodiagnosis. Its value as neurophysiologic investigative procedure has been known for many years but normal value of median and ulnar motor nerve was poorly reported in Korea. To evaluate of median and ulnar motor nerve terminal latency, amplitude of CMAP(compound muscle action potential), conduction velocity and F-wave latency for obtain clinically useful reference value. 71 normal volunteers(age,19-65 years;142 hands) examined who has no history of peripheral neuropathy, diabetic mellitus, chronic renal failure, endocrine disorders, anti-cancer medicine, anti-tubercle medicine, alcoholism, trauma, radiculopathy. Nicolet Viking Ⅱ was use for detected terminal latency, amplitude of CMAP, conduction velocity and F-wave latency of median and ulnar motor nerve. Data analysis was performed using SPSS. Descriptive analysis was used for obtain mean and standard deviation, independent t-test was used to compare between Rt and Lt side also compare between different in genders. The results are summarized as follows: 1. Median motor nerve terminal latency was right 3.00ms, left 2.99ms and there was no significantly difference between right and left side and genders. 2. Median motor nerve amplitude of CMAP was right 17.26mV, left 17.50mV and there was no significantly differences between right and left side and genders. 3. Median motor nerve conduction velocity was right 57.89m/sec, left 58.03m/sec and there was no significantly difference between right and left side and genders. 4. Median motor nerve F-wave latency was right 25.74ms, left 25.59ms and there was significantly differences between genders. 5. Ulnar motor nerve terminal latency was right 2.38ms, left 2.45ms and there was significantly differences between right and left side. 6. Ulnar motor nerve amplitude of CMAP was right 15.99mV, left 16.02mV and there was no significantly differences between right and left side and genders. 7. Ulnar motor nerve conduction velocity was right 60.35m/sec, left 59.73/sec and there was no significantly differences between right and left side and genders. 8. Ulnar motor nerve F-wave latency was right 25.53ms, left 25.57ms and there was significantly differences between genders.

척골 지단 신경의 전기생리학적 연구

김종순,이현옥,안소윤,구봉오,남건우,김호봉,류재관,류재문 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.2

The ulnar nerve extends down the arm, across the elbow, and into the hand. It provides sensation to the little and ring fingers and activates many of the small muscles in the hand. The determination of peripheral nerve conduction velocity is an important part of ulnar nerve evaluation. The electrodiagnostic value as neurophysiologic investigative procedure has been known for many years but normal value of digital nerve was not reported in korea. The purpose of this investigation was to measure the digital nerve conduction velocity of ulnar nerve for obtain clinically useful reference value and compare difference in each fingers and then compare with the other countries. 71 normal Korean volunteers (age, 19-65 years; 142 hands) examined who has no history of peripheral neuropathy, diabetic mellitus, chronic renal failure, endocrinedisorders, anti-cancer medicine, anti-tubercle medicine, alcoholism, trauma, radiculopathy. Nicolet Viking Ⅱ(EMG machine) was use for detected conduction velocity and amplitude of digital nerve in ulnar nerve. Data analysis was performed using SPSS. Descriptive analysis was used for obtain mean and standard deviation and independent t-test was used to compare with ring and little finger. Conduction velocity of the right ring finger was 57.44m/sec and little finger was 55.32msec. The left ring finger was 55.55msec and little finger was 54.11msec. Amplitude of the right ring finger was 30.28µV and little finger was 48.36µV. The left ring finger was 30.67µV and little finger was 57.76µV. There were significantly difference between ring and little in amplitude (p<.05) but there were no statistically difference between conduction velocity of ring and little finger (p>.05). The amplitude of little finger are greater than ring finger. The present results revealed that electodiagnosis can easily perform in little finger for digital nerve of ulnar nerve study.

Angiostatin Works as Immune Modulatory Molecules via Inhibition of Neutrophil Activation and Migration

Woo, So-Youn 대한미생물학회 2014 Journal of Bacteriology and Virology Vol.44 No.1

Angiostatin is derived from enzymatic degradation of plasminogen and it has endogenous anti-angiogenic properties. Although tumor cells, macrophages, platelets, and neutrophils generate high amount of angiostatin, its expression is increased in inflammatory conditions. Moreover, angiostatin binds to integrin ${\alpha}_V{\beta}_3$, ATP synthase, and angiomotin, which expressed on neutrophils. Activated neutrophils are essential to innate immune response, but also cause tissue damage through production of reactive oxygen species (ROS) and increase lifespan. In this article, it suggests several mechanism of angiostatin as immune regulator for neutrophils in inflammatory conditions; complex with integrin ${\alpha}_V{\beta}_3$ and $F_1F_0$ ATP synthase on lipid raft, attenuate polarization, and ROS production. These data provide possible exploit of double-edged role of neutrophils in acute inflammatory pathologies to preserve beneficial effect and minimize tissue damage.

Neutrophils in Immunity

So-Youn Woo 대한미생물학회 2012 Journal of Bacteriology and Virology Vol.42 No.2

Neutrophils in Immunity

Woo, So-Youn 대한미생물학회 2012 Journal of Bacteriology and Virology Vol.42 No.2

Neutrophils are the most abundant white blood cells in the peripheral blood and have long been recognized as the major phagocytes in acute infection by destroying extracellular pathogens. Although research on neutrophils hampered by intractability in the experiments, the newly discovered effector functions of neutrophils includes granular proteins, and cytokines, extracellular traps. With all effector mechanism neutrophils play a critical role in the pathogenesis of acute and chronic infection, autoimmunity and cancer.

Angiostatin Works as Immune Modulatory Molecules via Inhibition of Neutrophil Activation and Migration

So-Youn Woo 대한미생물학회 2014 Journal of Bacteriology and Virology Vol.44 No.1

Angiostatin is derived from enzymatic degradation of plasminogen and it has endogenous anti-angiogenic properties. Although tumor cells, macrophages, platelets, and neutrophils generate high amount of angiostatin, its expression is increased in inflammatory conditions. Moreover, angiostatin binds to integrin αvβ₃, ATP synthase, and angiomotin, which expressed on neutrophils. Activated neutrophils are essential to innate immune response, but also cause tissue damage through production of reactive oxygen species (ROS) and increase lifespan. In this article, it suggests several mechanism of angiostatin as immune regulator for neutrophils in inflammatory conditions; complex with integrin αvβ₃ and F₁F? ATP synthase on lipid raft, attenuate polarization, and ROS production. These data provide possible exploit of double-edged role of neutrophils in acute inflammatory pathologies to preserve beneficial effect and minimize tissue damage.

Epidemiological Investigation on Botulism of Cattle in Northern Gyeonggi-do between 2011 and 2012

Woo seog Jeong,Jeong Kyoung Bae,Woo Jin Jeon,Youn Ju Kim,Ha chung Yoon,Woo Seok Chang,Sung Chul Hwang,Jee Hye Kim,Yong Ju Lee,Myeong Soo Kim,Do Soon Kim,Yi Seok Joo,So Young Park,Oun Kyong Moon 대한수의학회 2012 대한수의학회 학술대회발표집 Vol.2012 No.-