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In Vitro Selection of Hammerhead Ribozymes with Optimized Stems I and III
Sim, So-Yeong,Kim, Se-Mi,Kim, Ha-Dong,Ahn, Jeong-Keun,Lee, Young-Hoon,Cho, Bong-Rae,Park, In-Won Korean Society for Biochemistry and Molecular Biol 1998 Journal of biochemistry and molecular biology Vol.31 No.2
A pool of cis-acting hammerhead ribozymes randomized in their substrate recognition sequences was constructed. A variety of active cis-acting ribozymes which had various structures of stems I and III was selected from the pool by in vitro selection. The selected ribozymes were cloned and sequenced. The relationship between the cleavage efficiency and base-pairing in stems I and III of the selected ribozymes was investigated. The ribozymes with the smaller difference in folding energies between the active conformation and the stable but inactive conformation showed a tendency to have the better cleavage efficiency. The optimum length of stem I was 5 or 6 bases while the longer stem III, in general, appeared to be required for efficient cleavage. The specificity of the ribozyme reaction is discussed in terms of the length of stems I and III.
심소영(So Yeong Sim),권연희(Yeon Hee Kwon) 한국보육지원학회 2021 한국보육지원학회지 Vol.17 No.4
Objective: The purpose of this study was to understand the teacher’s role as play supporter in a carry out play generated curriculum and develop and implement an action plan in order to indoor free play. Methods: Participants were 14 kindergartners (9 boys, 5 girls) and one teacher. Processes of planning-implementation and observation- reflection were repeated in a cyclical procedure and the 3rd action plan was conducted based on strategies to support children’s play. Results: Children experienced the following: Continuing and immersive play, play filled with experiments and challenges, play to make together, attitude to enjoy novelty and respect differences, and play of trial and error. And the researcher changed the following: Be sensitive to the child s words and actions, finding the ‘real interest’ of children in ‘waiting’, acquiring the perception that ‘children are the masters of play’, seeing the value of learning in children s expressive play, and gain confidence in play support. Conclusion/Implications: This study suggested the need for teacher to have patience and demonstrate reflective thinking in order to support children s play.
대한간학회지 제8차 춘계학술대회 초록집 : 포스터 전시 ; 간의 염증성 가성 종양 1예
장재영 ( Jang Jae Yeong ),김연수 ( Kim Yeon Su ),천영국 ( Cheon Yeong Gug ),김영석 ( Kim Yeong Seog ),김정훈 ( Kim Jeong Hun ),문종호 ( Mun Jong Ho ),조영덕 ( Jo Yeong Deog ),진소영 ( Jin So Yeong ),심찬섭 ( Sim Chan Seob ),김부성 대한간학회 2002 Clinical and Molecular Hepatology(대한간학회지) Vol.8 No.2(S)
배소영(So Yeong Pae),장유경(You Kyung Chang Song),곽금주(Keum Joo Kwak),성현란(Hyun Ran Sung),심희옥(Hee Og Sim) 한국언어청각임상학회 2004 Communication Sciences and Disorders Vol.9 No.1
MCDI-K를 통해 본 한국유아의 표현어휘발달과 성차. ?언어청각장애연구?, 2004, 제9권, 제1호, 45-56. 이 연구는 한국유아의 어휘발달 평가 규준자료를 마련하기 위한 기초연구로 표현어휘 발달수준을 월령별로 알아보고, 어휘습득에서의 성차이를 살펴보고자 하였다. 연구대상은 18개월에서 36개월에 이르는 한국유아 1,138명이었으며, 서울, 경기, 호남, 영남, 충청 지역의 아동을 포함하였다. 연구방법의 경우 표현어휘 측정은 유아용 MCDI-K를 이용하였는데 각 월령별로 약 60명의 어머니들의 보고에 의해 아동의 표현어휘 수를 측정하였다. 연구결과 18개월, 24개월, 30개월, 36개월 남아의 경우 각각 평균 79낱말, 253낱말, 365낱말, 504낱말을 산출하는 것으로 나타났다. 한편 18개월, 24개월, 30개월, 36개월 여아의 경우 각각 평균 74낱말, 312낱말, 421낱말, 508낱말을 산출하는 것으로 나타났다. 이 결과는 유아용 MCDI-K가 한국유아의 낱말발달에 대한 양적 증가를 타당하게 반영하고 있음을 지지한다. 한국유아들도 각 월령에서 표준편차가 크게 나타난 것으로 보아 표현낱말수에는 개인차가 크게 작용하고 있음을 알 수 있다. 남,여 성차이를 살펴본 결과, 21, 22, 23, 25, 33, 34개월의 6개 월령집단의 경우에 여아들이 남아들보다 더 많은 수의 낱말을 산출하는 것으로 나타났지만, 기타 월령대에서는 통계적으로 의의있는 차이를 나타내지는 않았다. MCDI-K가 표현언어발달지체 판별을 위한 자료로 사용되기 위한 시사점도 살펴보았다. This study investigated Korean preschoolers’ expressive word development and the gender differences in the development of semantics. The Korean adaptation of the MacArthur Communicative Development Inventories (MCDI-K) was used for the analysis. The mothers of 1,138 preschoolers from 5 different provinces in Korea provided the language data of their children. Mothers reported their children’s expressive words in the vocabulary checklist, MCDI-K(Pae, 2003) composed of 641 Korean words. The children were divided into monthly age groups, each group comprising 60 children, 30 girls and 30 boys. It was found that the vocabulary size increased as the children get older. It was also found that there are gender differences, which are statistically significant, between girls and boys in the monthly ages of 21, 22, 23, 25, 33, and 34. The implications of the results are discussed as to the usefulness of MCDI-K for the identification of expressive-language impairments for Korean children.
CHO, So Yean,SIM, Joon-Soo,JEONG, Choon Sik,CHANG, Seung Yeup,CHOI, Don Woong,TOIDA, Toshihiko,KIM, Yeong Shik 德成女子大學校 藥學硏究所 2004 藥學論文誌 Vol.15 No.1
In order to evaluate the improvement in the treatment of chronic arthritis, we investigated chondroitin sulfate depolymerization product (low molecular weight chondroitin sulfate, LMWCS) and intact chondroitin sulfate (CS) in vitro and in vivo. LMWCS was prepared by a chemical depolymerization process induced by hydrogen peroxide in the presence of copper salts. LMWCS (300㎎/㎏) and CS (1200㎎/㎏) were orally administered to DBA/1J mice once daily for 14d prior to initial immunization with type Ⅱ collagen. Their elastase activities and the production of cytokines in sera were examined on type Ⅱ collagen-induced arthritis in DBA/1J mice. We also compared the paracellular transport of LMWCS and CS across Caco-2 cell monolayers and examined the inhibitory effects on elastase activities. LMWCS inhibited elastase activity slightly, but CS did not show inhibition. Hind paw edema was significantly decreased by LMWCS treatment. Levels of anti-type I1 collagen antibody and tumor necrosis factor-alpha (TNF-α) in sera were also reduced by LMWCS treatment but not in case of CS, although no significant difference was observed between LMWCS and CS on interleukin-6 (IL-6) induction. The LMWCS preparation showed preventive effects on the type Ⅱ collagen-induced arthritis in DBA/1J mice and better permeability through Caco-2 cells.
Enhancement of Heparin and Heparin Disaccharide Absorption by the Phytolacca americana Saponins
Cho, So-Yean,Sim, Joon-Soo,Kang, Sam-Sik,Jeong, Choon-Sik,Linhardt, Robert-J,Kim, Yeong-Shik The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.12
We studied the effects of phytolaccosides, saponins from Phytolacca americana, on the intestinal absorption of heparin in vitro and in vivo. The absorption enhancing activity of these compounds (phytolaccosides B, $D_2$, E, F, G and I) was determined by changes in transepithelial electrical resistance (TEER) and the transport amount of heparin disaccharide, the major repeating unit of heparin, across Caco-2 cell monolayers. With the exception of phytolaccoside G, all of them decreased TEER values and increased the permeability in a dose-dependent and time-dependent manner. In vitro, phytolaccosides B,$D_2$, and E showed significant absorption enhancing activities, while effects by phytolaccoside F and I were mild. In vivo, phytolaccoside E increased the activated partial thromboplastin time (APTT) and thrombin time, indicating that phytolaccoside E modulated the transport of heparin in intestinal route. Our results suggest that a series of phytolaccosides from Phytolacca americana can be applied as pharmaceutical excipients to improve the permeability of macromolecules and hydrophilic drugs having difficulty in absorption across the intestinal epithelium.
COVID-19 팬데믹에서 Airbnb 호스트의 마케팅 전략의 변화가 공유성과에 미치는 영향
김소영(Kim So Yeong),심지환(Sim Ji Hwan),정여진(Chung Yeo Jin) 한국지능정보시스템학회 2021 지능정보연구 Vol.27 No.3
전 세계적인 COVID-19의 유행으로 인해 관광산업 전반이 큰 타격을 받고 있다. 최근 공유경제의 확산으로 팽창되고 있는 Airbnb와 같은 숙박 공유서비스는 공급자와 수요자 간의 신뢰와 소통을 기반으로 거래가 이루어지기 때문에 팬데믹으로 인한 영향을 특히 크게 받고 있다. 팬데믹 상황이 개인의 여행에 대한 인식과 행동을 변화시킴에 따라 이를 개선하기 위한 전략에 대한 논의가 이루어지고 있지만 대부분의 연구는 전통적인 외식업, 숙박업 공급자와 정부 측면의 거시적 전략을 제시하고 있다. 본 연구는 Peer-to-Peer 거래 중심의 공유경제의 특수성을 고려하여 COVID-19 팬데믹 발생 전후로 Airbnb 개별 호스트의 마케팅 전략의 변화가 공유성과에 미치는 영향을 실증적으로 분석함으로써 개별 호스트 측면의 팬데믹 전략에 대해 논한다. Airbnb의 호스트가 본인의 시설을 홍보하는 통로인 시설소개 텍스트를 수집하여 딥러닝 기반 특성추출방법인 Attention-based aspect extraction 모델로부터 9개의 주요 특성을 추출하였다. 추출된 특성이 해당 텍스트에서 등장하는 빈도가 COVID-19 발생 전후 변화량을 측정하여 이것이 공유성과에 미치는 영향을 분석하였다. 또한 이러한 영향을 숙박시설의 유형 간에 비교함으로써 시설 유형별 효과적으로 작용하는 특성을 관찰하였다. 회귀분석 결과 주방시설, 정원, 호스트와의 교류 순으로 공유성과에 긍정적인 영향을 보이지만 시설 유형에 따라 공유성과에 미치는 영향은 다소 차이가 있었다. 특히 집 전체를 대여하는 경우 개인실 대여에 비해 주방시설에 대한 설명이 상당한 효과를 보여주었다. 이를 통해 본 연구는 공유숙박 서비스의 개별 서비스 제공자가 시설의 종류에 따라 취할 수 있는 팬데믹 위기 대처전략에 대한 아이디어를 제시한다. The entire tourism industry is being hit hard by the COVID-19 as a global pandemic. Accommodation sharing services such as Airbnb, which have recently expanded due to the spread of the sharing economy, are particularly affected by the pandemic because transactions are made based on trust and communication between consumer and supplier. As the pandemic situation changes individuals" perceptions and behavior of travel, strategies for the recovery of the tourism industry have been discussed. However, since most studies present macro strategies in terms of traditional lodging providers and the government, there is a significant lack of discussion on differentiated pandemic response strategies considering the peculiarity of the sharing economy centered on peer-to-peer transactions. This study discusses the marketing strategy for individual hosts of Airbnb during COVID-19. We empirically analyze the effect of changes in listing descriptions posted by the Airbnb hosts on listing performance after COVID-19 was outbroken. We extract nine aspects described in the listing descriptions using the Attention-Based Aspect Extraction model, which is a deep learning-based aspect extraction method. We model the effect of aspect changes on listing performance after the COVID-19 by observing the frequency of each aspect appeared in the text. In addition, we compare those effects across the types of Airbnb listing. Through this, this study presents an idea for a pandemic crisis response strategy that individual service providers of accommodation sharing services can take depending on the listing type.