리튬-필로카르핀으로 유발된 경련중첩증 흰쥐에서 뇌량절개술의 효과

전신수,홍용길,박춘근,김문찬,최창락,강준기 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.5

정신분열병 환자에서 얼굴 영상의 작동기억 장애 : 기능자기공명영상연구

이창욱,김태석,주라형,박주미,박여진,김종진,전신수,배치운,김정진,이수정,이철,백인호 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.2

Objectives : Impaired processing of facial information is one of the broad ranges of cognitive deficits seen in patients with Schi-zophrenia. We aimed to elucidate the differences in brain activities involved in the process of facial working memory between schizophrenic patients and healthy comparison subjects. Methods : Twelve patients with schizophrenia were recruited along with twelve demographically matched healthy volunteers as a comparison group. Functional magnetic resonance imaging (fMRI) was used to assess cortical activities during the Perfor-mance of a 2-back working memory paradigm using images of neutral faces as mnemonic content. Rcsults : The patient group performed the tasks with reduced accuracy. Group analysis revealed that the left fusiform gyrus, the right superior frontal gyrus, the bilateral middle frontal gyri/insula, the left middle temporal gyrus, the precuneus, the quadrangular lobules and the vermis of cerebellum showed decreased cortical activities in the patient group. On the other hand, an increased level of activation in the lateral prefrontal cortex and the pahetal lobule was observed from the patient group, all in the right hemisphere. Conulusion : A decreased level of activity in the left fusiform gyrus among the patient group implicates inefficient processing of facial information. An increased level of activation in prefrontal and parietal neural networks from the patient group confirms earlier findings on the impaired working memory of patients with schizophrenia.

The Phosphorylation Status of Merlin Is Important for Regulating the Ras-ERK Pathway

Sin-Soo Jeun,Ju Ri Jung,Hongtae Kim,이주용,Eun-Jeoung Koh,Cheol Ji 한국분자세포생물학회 2005 Molecules and cells Vol.20 No.2

The neurofibromatosis type2 (NF2) tumor suppressor gene product, merlin, is structurally related to the ezrin- radixin-moesin (ERM) family of proteins that anchor the actin cytoskeleton to specific membrane proteins and participate in cell signaling. However, the basis of the tumor suppressing activity of merlin is not well understood. Previously, we identified a role of merlin as an inhibitor of the Ras-ERK signaling pathway. Recent studies have suggested that phosphorylation of merlin, as of other ERM proteins, may regulate its function. To determine whether phosphorylation of merlin affects its suppression of Ras-ERK signaling, we generated plasmids expressing full-length merlin with substitutions of serine 518, a potential phosphorylation site. A substitution that mimics constitutive phosphorylation (S518D) abrogated the ability of merlin to suppress effects of the Ras-ERK signaling pathway such as Ras-induced SRE transactivation, Elkmediated SRE transactivation, Ras-induced ERK phosphorylation and Ras-induced focus formation. On the other hand, an S518A mutant, which mimics nonphosphorylated merlin, acted like wild type merlin. These observations show that mimicking merlin phosphorylation impairs not only growth suppression by merlin but also its inhibitory action on the Ras-ERK signaling pathway.

Acupuncture stimulation for motor cortex activities: a 3T fMRI study.

Jeun, Sin-Soo,Kim, Jeong-Seok,Kim, Bum-Soo,Park, Sang-Dong,Lim, Eun-Chul,Choi, Gi-Soon,Choe, Bo-Young Institute for Advanced Research in Asian Science a 2005 The American journal of Chinese medicine Vol.33 No.4

<P>The acupoint, GB34, located in the back of the knee, is known to be effective in recovering motor function after a stroke. This study uses a functional magnetic resonance imaging (fMRI) study with 3T scanner to investigate whether or not acupuncture of GB34 produces a significant response of the modulation of somatomotor areas. A fMRI of the whole brain was performed in ten normal healthy subjects during two task stimulations of acupuncture manipulation on GB34 and sham points, inserting and twisting the needle for 25 seconds at a rate of approximately 120 times per minute; the needle manipulation was paused for a duration of 25 seconds as a control state. The process was repeated four times to have four epochs of stimulation. Bilateral sensorimotor areas (BA 3, 4, 6 and 7) showed approximately 6% of stimulation-related BOLD signal contrast, whereas very few areas were activated when sham stimulation was given. Acupuncture stimulation in GB34 modulates the cortical activities of the somatomotor area in humans. The present findings may shed light on the CNS mechanism of motor function by acupuncture, and form a basis for future investigations of motor modulation circuits in stroke patients.</P>

Radiation-induced brain injury

Dong-Soo Lee,Mina Yu,Hong-Seok Jang,Yeon-Sil Kim,Byung-Ock Choi,Young-Nam Kang,Youn-Soo Lee,Dong-Chul Kim,Yong-Kil Hong,Sin-Soo Jeun,Sei-Chul Yoon 대한방사선종양학회 2011 Radiation Oncology Journal Vol.29 No.3

Purpose: This study was designed to determine the influencing factors and clinical course of pathologically proven cases of radiation-induced brain injury (RIBI). Materials and Methods: The pathologic records of twelve patients were reviewed; these patients underwent surgery following radiotherapy due to disease progression found by follow-up imaging. However, they were finally diagnosed with RIBI. All patients had been treated with 3-dimensional conventional fractionated radiotherapy and/or radiosurgery for primary or metastatic brain tumors with or without chemotherapy. The histological distribution was as follows: two falx meningioma, six glioblastoma multiform (GBM), two anaplastic oligodendroglioma, one low grade oligodendroglioma, and one small cell lung cancer with brain metastasis. Results: Radiation necrosis was noted in eight patients and the remaining four were diagnosed with radiation change. Gender (p = 0.061) and biologically equivalent dose (BED)3 (p = 0.084) were the only marginally influencing factors of radiation necrosis. Median time to RIBI was 7.3 months (range, 0.5 to 61 months). Three prolonged survivors with GBM were observed. In the subgroup analysis of high grade gliomas, RIBI that developed <6 months after radiotherapy was associated with inferior overall survival rates compared to cases of RIBI that occurred ≥6 months (p = 0.085). Conclusion: Our study demonstrated that RIBI could occur in early periods after conventional fractionated brain radiotherapy within normal tolerable dose ranges. Studies with a larger number of patients are required to identify the strong influencing factors for RIBI development.

전방상소뇌 종양에 대한 후두경천막접근법

윤완수(Wan-Soo Yoon),이근수(Kun-Soo Lee),전신수(Sin-Soo Jeun) 대한두개저학회 2008 대한두개저학회지 Vol.3 No.2

Objectives : To minimize the retraction of the cerebellum and access the feeding artery directly, we used the occipital transtentorial approach for the anterosuperior cerebellar tumors. Here, we describe the surgical technique and the advantage of occipital transtentorial approach for anterosuperior cerebellar tumors. Methods : From January 1999 to December 2007, we performed the occipital transtentorial approach for 3 patients with infratentorial tumors: one cerebellar hemangioblastoma, one metastatic cerebellar tumor, and one transtentorial meningioma. All tumors were located at anterosuperior cerebellum. Digital subtraction angiography was performed to hemangioblastoma patient. We routinely checked visual acuity and visual field postoperatively. Results : All tumors were removed totally. One patient had transtentoiral meningioma showed transient hemianopsia postoperatively but improved spontaneously. There was no severe morbidity and mortality. Conclusions : Despite of the limited experience, occipital transtentorial approach is a useful surgical strategy for the removal of tumors located at the anterosuperior cerebellar tumors. To prevent the neurologic complication, excessive occipital lobe retraction should be avoided.

Overexpression of CXC Chemokine Receptors Is Required for the Superior Glioma-Tracking Property of Umbilical Cord Blood-Derived Mesenchymal Stem Cells

Kim, Dal-Soo,Kim, Ji Hyun,Kwon Lee, Jae,Choi, Soo Jin,Kim, Jae-Sung,Jeun, Sin-Soo,Oh, Wonil,Yang, Yoon Sun,Chang, Jong Wook Mary Ann Liebert 2009 STEM CELLS AND DEVELOPMENT Vol.18 No.3

<P>Our observations indicate that umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) have a strong migration capacity toward the human glioma cell line, U-87 MG, LN18, U138, and U251, when compared to several other cancer cell lines. In order to identify soluble factors that function to attract UCB-MSCs, we used cytokine antibody arrays to screen changed cytokines in conditioned media from U-87 MG cells. Among these, interleukin-8 (IL-8) and growth-related oncogene (GRO-alpha) enhanced UCB-MSC migration. Furthermore, antibodies treatment against the IL-8 receptors reduced these migration events and overexpression of IL-8 in cells with lower level of IL-8 such as A549 could induce UCB-MSC migration. Since we found that the capacity of UCB-MSC migration is much higher than that of bone marrow-derived MSCs (BM-MSCs) toward either U-87 MG cells or recombinant IL-8, we compared the levels of the IL-8 receptor, CXC chemokine receptor 1 (CXCR1) and CXCR2 between two kinds of MSCs by RT-PCR and immunostaining. Expression levels of two receptors were much higher in UCB-MSCs than in BM-MSCs. These data suggest that higher levels of two IL-8 receptors could influence downstream signaling events affecting superior UCB-MSC migration toward the glioma cells.</P>

Radiation-induced brain injury: retrospective analysis of twelve pathologically proven cases

Lee, Dong-Soo,Yu, Mi-Na,Jang, Hong-Seok,Kim, Yeon-Sil,Choi, Byung-Ock,Kang, Young-Nam,Lee, Youn-Soo,Kim, Dong-Chul,Hong, Yong-Kil,Jeun, Sin-Soo,Yoon, Sei-Chul The Korean Society for Radiation Oncology 2011 Radiation Oncology Journal Vol.29 No.3

Purpose: This study was designed to determine the influencing factors and clinical course of pathologically proven cases of radiation-induced brain injury (RIBI). Materials and Methods: The pathologic records of twelve patients were reviewed; these patients underwent surgery following radiotherapy due to disease progression found by follow-up imaging. However, they were finally diagnosed with RIBI. All patients had been treated with 3-dimensional conventional fractionated radiotherapy and/or radiosurgery for primary or metastatic brain tumors with or without chemotherapy. The histological distribution was as follows: two falx meningioma, six glioblastoma multiform (GBM), two anaplastic oligodendroglioma, one low grade oligodendroglioma, and one small cell lung cancer with brain metastasis. Results: Radiation necrosis was noted in eight patients and the remaining four were diagnosed with radiation change. Gender (p = 0.061) and biologically equivalent dose $(BED)_3$ (p = 0.084) were the only marginally influencing factors of radiation necrosis. Median time to RIBI was 7.3 months (range, 0.5 to 61 months). Three prolonged survivors with GBM were observed. In the subgroup analysis of high grade gliomas, RIBI that developed <6 months after radiotherapy was associated with inferior overall survival rates compared to cases of RIBI that occurred ${\geq}6$ months (p = 0.085). Conclusion: Our study demonstrated that RIBI could occur in early periods after conventional fractionated brain radiotherapy within normal tolerable dose ranges. Studies with a larger number of patients are required to identify the strong influencing factors for RIBI development.

Efficient Intracytoplasmic Labeling of Human Umbilical Cord Blood Mesenchymal Stromal Cells with Ferumoxides

Lee, Jae Kwon,Lee, Man Kyoung,Jin, Hye Jin,Kim, Dal-Soo,Yang, Yoon Sun,Oh, Wonil,Yang, Sung-Eun,Park, Tae Seok,Lee, Soo Yeol,Kim, Bum-Soo,Jeun, Sin-Soo Cognizant Communication Corp. 2007 CELL TRANSPLANTATION Vol.16 No.8

<P>Mesenchymal stromal cells (MSCs) are multipotent cells found in several adult tissues; they have the capacity to differentiate into mesodermal, ectodermal, and endodermal tissues in vitro. There have been several reports that MSCs have therapeutic effects in a variety of diseases. Therefore, using a cell labeling technique, monitoring their temporal and spatial migration in vivo, would be useful in the clinical setting. Magnetic resonance imaging (MRI)--tracking of superparamagnetic iron oxide (SPIO)-labeled cells--is a noninvasive technique for determining the location and migration of transplanted cells. In the present study, we evaluated the influence and toxicity of SPIO (ferumoxides) labeling on multiple differentiated MSCs. To evaluate the influence and toxicity of ferumoxides labeling on differentiation of MSCs, a variety of concentrations of ferumoxides were used for labeling MSCs. We found that the cytoplasm of adherent cells was effectively labeled at low concentrations of ferumoxides. Compared with unlabeled controls, the ferumoxides-labeled MSCs exhibited a similar proliferation rate and apoptotic progression. The labeled MSCs differentiated into osteoblasts and adipocytes in an identical fashion as the unlabeled cells. However, chondrogenesis and neurogenesis were inhibited at high concentrations of ferumoxides. Our results suggest the effective concentration for ferumoxides use in tracking MSCs.</P>

뇌실질내 출혈의 보존적 및 수술적 치료의 비교분석

전신수,이길송,조경근,지철,나형균,송진언,최창락 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.1

Retrospective analysis of 46 patients with intracerebral hematoma showed that the attack was most frequent in sixth decade and more prevalent in female. The most common cause of the attack was hypertension(80%) and the site of hemorrhage was putamen 32%, thalamic area 15%, subcortical area 17%, cerebellum 17%, ventricle 13%, and brain stem 4%. Mortality of total cases was 36% and there was no difference of mortality in both conservatively or operatively treated group(38% in conservative group, and 35% in operative group). The prognosis of the patient was unfavorable in the group of poor pretreatment Glasgow coma scale(GCS) and those of cases demonstrated more than 30㏄ of hematoma on computerized tomography(CT) of the brain. The improvement of GCS after management was better in operative group than in the conservative group.