흰쥐 피부의 11β-hydroxysteroid dehydrogenase 분포양상

류시윤,정규식,김성호 대한해부학회 1996 Anatomy & Cell Biology Vol.29 No.4

오리 선위 점액분비세포의 점액질에 대한 조직화학적 연구

류시윤,김홍선,김무강,신태균,이영호 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

Histochemical sequential staining techniques, PAS, alcian blue pH 2.5 and pH 0.4, were applied to sections from the proventriculus of ducks to observe the histochemical properties of epithelial mucosubstances in the proventriculus of mallard and white Pekin ducks. The whole proventriculus obtained were divided systemically cross strips and processed routinely for histologic and histochemical study. Base on the histologic characteristics, we designated as upper portion which is the distal region of the esophageal-proventriculus junction, as middle portion, and as lower portion which is the proximal region of the proventricular-isthmus junction. The results obtained are as follows 1. Generally in the surface and foveolar epithelia showed the presence of the mixture of neutral and acid mucosubstances. 2. In the tubular gland showed the acidic nonsulfated and sulfated mucosubstance in addition to neutral mucosubstances with occasional neutral and acidic nonsulfated mucosubstances. 3. In the compound gland neutral mucosubstance with scant amounts of acidic mucosubstances or with lack of it were demonstrated. 4. There were recognized differences between speies in the staining intensity of all three types of mucosubstances. 5. There were recognized topographic difference in the acidity of epithelial mucosubstances according to portion.

농장 사육되는 타조에서 나타난 다리 기형의 증례 보고

최종윤,손화영,전무형,조성환,김혜성,류시윤,박배근,이영원,윤원기 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

Two farmed young ostrich chicks were applied to pathology laboratory at Chungnam National University. Grossly, one ostrich showed rotated tibiotarsus, the other showed rolled toes. Case 1 ostrich was hypertrophy of fibualis longus and tibial cranialis tendon and gastrocnemius tendon in crus, inflammation of flexor digitorum longus. In histopathological views, myocytes of fiburalis longus were showed hypertrophy and hyperplasia. It is not found any specific feature in X-ray of the case 2 ostrich. Limb deformities were detected frequently in farmed ostrich chicks in 2- to 3 weeks old. Limb deformities were an important cause of mortality in farmed ostrich chicks during the first 10weeks after hatch. Cause of limb deformities were considered a nutrition problem.

연쇄상구균을 실험감염시킨 넙치치어의 병리조직학적 관찰

조성환,류시윤,김현수,박배근,김덕환,손화영,윤원기,신창호 충남대학교 수의과대학 동물의과학연구소 1999 動物醫科學硏究誌 Vol.7 No.-

Pathological observations were examined after abdominal inoculation in 7∼8㎝ sized flounder (Paralichthys olivaceus) with 1X10^7 cfu/㎖ of Streptococcus faecalis. The infected flounder were clinically observed and weighed a fish body at 0, 5, 10, 15, 20, 25, 30 days post inoculation. In infected group, mortality were 70% and feed efficiency were 7.2%. In non-infected group, mortality were 10% and feed efficiency were 18.2%. Macroscopically, the significant lesions were characterized by protrusions and opacity of the eyes and ulcer of the caudal pecuncle. Histopathologically, the lesions were observed a degeneration. necrosis and inflammatory cells in muscle of the caudal peduncle and a degeneration and necrosis of the rod cells and detached retina from the black pigmented layer

분화중인 흰쥐 콩팥의 요세관에서 nNOS의 발현

송지현,류시윤,김진,정주영 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.2

Nitric oxide는 콩팥에서 혈류의 조절, 사구체 혈액역동학 및 요세관의 운반에 중요한 역할을 하는 인자로서 최근 세포의 주기조절, 유사분열에도 관여하는 것으로 알려져 있다. 최근 연구결과에 의하면 발생중인 콩팥에서의 nNOS의 발현은 성체와는 다른 것으로 알려져 있으나, 발생중인 콩팥에서의 발현 양상 및 발현시기 등에 대해서 알려진 바 없다. 따라서 본 연구에서는 발생중인 콩팥을 이용하여 분화중인 요세관에서 nNOS의 발현양상을 관찰하고자 하였다. 성체에서 nNOS의 면역반응성은 사구체 보우만주머니의 벽측상피와 치밀반, 내림가는부분 및 수질집합관에 발현되었다. 발생중인 콩팥에서는 임신 15일에 처음 발현하기 시작하였으며, 그 부위는 원위세관의 굵은오름부분이었다. 임신 20일에서 출산후 14일까지 새로이 형성되는 요세관의 굵은오름부분에서 관찰되었으나, 분화를 마친 요세관에서는 그 발현 양상이 감소하였다. 출생후 21일경에는 최종적으로 치밀반에만 국한되어 발현되었다. 속수질에서는 내림가는 부분과 속수질 집합관에서 발현되었으며 그 양상은 수질 중간부위에서 강하게 발현되었으며 시작부위와 말단부위에서는 약하게 발현되었다. 이상의 결과로 보아 굵은오름부분에서의 nNOS의 발현은 요세관의 분화정도와 일치하며 특히 그 발현양상이 분화가 활발한 시기에 강하게 발현되고 분화가 저하된 후에는 발현이 감소하는 것으로 보아 발생중인 콩팥의 성장에 중요한 역할을 할 것으로 생각된다. Nitric oxide (NO) is an important regulator of renal blood flow, glomerular hemodynamics, and tubule transport processes in the kidney. There is also evidence that NO is involved in cell cycle regulation and mitotic division. During development the nNOS expression pattern differs from that observed in adult animals. However, little is known about temporal and spatial patterns of nNOS expression in the developing kidney. The purpose of this study was to establish the time of expression and the distribution of nNOS in the developing rat kidney. Kidneys from 14-, 16-, 17-, 18-, and 20-day-old fetuses, 1-, 4-, 7-, 14-, and 21-day-old pups, and adult animals were preserved and processed for immunohistochemistry. In the adult kidney, nNOS was detected in the parietal epithelium of Bowman’s capsule, macula densa, descending thin limb and inner medullary collecting duct. nNOS immunoreactivity appeared first in the distal tubule anlage at 15 days of gestation, and in all epithelial cells of developing thick ascending limbs (TAL) as well as macula densa of 17- and 18-day-old fetuses. From 20 days of gestation to 14 days after birth, nNOS was expressed in the newly formed cortical TAL, which are located in the medullary ray, whereas in mature TAL of juxtamedullary nephrons, nNOS immunolabeling gradually decreased in intensity and became restricted to the macula densa. In inner medullary collecting ducts, nNOS immunoreactivity appeared first at 7 days after birth in the papillary tip and gradually ascended to the border between outer and inner medulla. In the descending thin limb and parietal epithelium of Bowman’s capsule, weak nNOS immunoreactivity was observed at 14 days after birth and labeling gradually increased to adult levels at 21 days after birth. These results suggest that differential expression of nNOS in the developing kidney is an important physiological regulator of renal function during kidney maturation.

5-Fluorouracil이 생쥐소장점막의 위축에 미치는 영향에 대한 형태계측학적 관찰

김홍선,류시윤 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.1

This experiment was carried out to observe the effect of 5-fluorouracil on the mucosa of small intestine in mice. Adult mice received 40-200mg/kg 5-fluorouracil by the intraperitonial injection and the subsequent morphological effects on the ileum at three, six, twelve, and twenty-four hours after single injection and at twenty-four hours after injection for three days were evaluated using morphometry and light microscope. 1. By six hours after injection the reductions in area occupied by the mucosa per unit length of histological section in proportion to a dose were marked difference as 39.78%, 51.42% repectively but after twelve hours showed no significant difference. 2. The average area occupied by the villus including the core of lamina propria by twelve hours in the received 40mg/kg were gradually reduced, a reduction amount 27.62% and at twenty-four hours were maximal reduced, 48.36% and in the received 200mg/kg, from three hours the reduction were marked except at twelve hours. 3. In the average height of the villus, a similar decrease tendency as the average area occupied by the villus was observed. 4. The reduction of the area occupied by the villus and the height of the villus was related to a dose but not proportion to the times of injection. Evidence from the above results has indicated that the rapid atrophy of the intestinal mucosa were caused to the mainly crypts by twelve hours after injection and the villi as well as the crypts at twenty-four hours.

돼지의 전염성 흉막 폐렴의 병리조직학적 관찰

문지영,손화영,류시윤,조성환 忠南大學校 獸醫科大學 動物醫科學硏究所 2005 動物醫科學硏究誌 Vol.13 No.-

6 pigs suddenly died of severe dyspnea at the piggery in Chungbuk and Daejeon, Korea. Authors diagnosed it as porcine pleuropneumonia resulting in A. pleuropneumoniae on the ground of pathological findings and bacteriological isolation. The clinical signs were high fever, depression, dyspnea, blood-tinged discharge through the mouth and nostrils, cough, sialism. In grossly, congestion and hemorrhage were showed in the acute pleuropneumonia in 4 pigs. In 2 pigs, the chronic pleuropnuemonia characterized by adhesion between visceral pleura and parietal pleura resulting in fibrous exudate was observed. The histopathologic changes were characterized by congestion, hemorrhage, neutrophil infiltration, edema, vascular thrombosis, macrophage activation, and fibrinous exudate in the acute pleuropneumonia. In the chronic stages of this disease, fibrous pleuritis, macrophage infiltration, lymphocyte infiltration, and marked fibrosis around areas of necrosis were observed. Also there were oat cells, the characters of porcine pleuropneumonia. Actinobacillus pleuropneumonia was isolated from pneumonic lesions and biochemical identification was carried out. A. pleuropneumoniae was particularly susceptible in vitro to Amoxicillin/clavulanic acid, Ampicillin, Ciprofloxacin. Norfloxacin, Gentamycin, Tiamulin, Cefazolin, Ceftiofur, and Colistin.

푸들에서 발생한 악성혼합유선종양

박수진,조성환,류시윤,손화영 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A 2cm sized solid mass was observed in the right inguinal lesion of a 10-years old poodle bitch. Radiological finding in the mass showed higher density than that of soft tissue and thoracic radiograph showed pulmonary interstitial pattern. Gross findings appeared a yellowish-brown solid mass containing cysts filled with brown colored mucoid fluids in the udder. And the lung showed multiple nodules scattered throughout the pulmonary parenchyma. In light microscopic finding, mass contained neoplastic proliferation of glandular epithelial and myoepithelial cells and showed metaplstic lesions of these cells to chondrous tissue. Glandular epithelial cells were undifferentiated and polygonal myoepithelial cells were frequently embedded in or surrounded by a pink to light blue mucinous matrix. Tumor cells of the pulmonary nodule had resemblance to neoplastic proliferation of glandular epithelial and myoepithelial cells observed in the mammary gland mass. Based on the gross and microscopic findings, the mass was diagnosed as malignant mixed tumor with metastasis to the lung.

마우스에서 오존수의 급여가 쥐와포자충 감염에 미치는 영향

박배근,김덕환,류시윤,김현수,조성환,전무형 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

Ozone is very effective in acute and chronic viral disease as a virucidal agent in reversing positive antibody tests and in improving the general health of the animals. The effects of ozonized water supply against Cryptosporidium muris was studied in 5-weeks-old mice. After oral inoculation of mice with 1×10^6 oocysts, ozonized water(0.5ppm) and tap water were supplied to experimental and control group, respectively. The prepatent period of both group was 8 days postinoculation(PI). The patent period of experimental group was longer(62days) than that of experimental group(57days). The number of oocysts in the feces were maxium on days 28 PI, indicating 3.54×10^6 oocysts/experimental mouse and 5.56×10^6 oocysts/ control mouse. The number of discharge oocysts during 12-33 PI was more in control group than that of experimental group. In the view of gastric gland section on days 28 PI, the number of endogenous development of C muris was smaller in experimental group than in control group. This results indicates that the autoinfected sporozoite were damaged by ozone.

개의 대퇴골간에서 발생한 유합부전의 1례

강은경,조성환,류시윤,손화영 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Fracture healing is a complex physiological process that involves the coordinated participation of several cell types. Unlike other tissues that heal by the formation of poorly organized scar, in fracture healing the original tissue, bone, is regenerated and the properties of the preexisting tissue largely are restored. Fracture healing generally proceeds in an orderly fashion when appropriate biological and mechanical component are present. Many complications may occur with repair and subsequent healing of fracture, including osteomyelitis, implant loosening, and fracture instability. All of these complications may result in delayed union, nonunion or malunion of the fracture. A 1-year-old male, Collie sustained a closed, transverse femoral fracture in a mortor vehicle accident. His initial treatment with open reduction and bone plating was failed. A second surgery was done to remove the plate and perform corrective osteotomy, and bone grafting. Histopathological observation of the bone fragment from fracture end was carried.