DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

한국인 폐확산능 정상예측식의 임상적 유용성과 정확성

나승원 ( Seung Won Ra ),박태선 ( Tai Sun Park ),홍윤기 ( Yoon Ki Hong ),홍상범 ( Sang Bum Hong ),심태선 ( Tae Sun Shim ),임채만 ( Chae Man Lim ),이상도 ( Sang Do Lee ),고윤석 ( Youn Suck Koh ),김우성 ( Woo Sung Kim ),김동순 ( Dong 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.64 No.2

연구배경: 폐확산능을 해석하는 데 필요한 정상예측식으로는 한국인을 대상으로 하여 박 등이 개발한 식(박 식)이 있으나 아직 외국 정상예측식을 많이 사용하고 있다. 이에 국내에서 많이 사용하는 외국 정상예측식인 Burrows 식과 박 식의 임상적 유용성과 정확성을 비교하고자 하였다. 방법: 1. 임상적 유용성 연구; 2006년 7월부터 12월까지 6개월간 폐확산능검사를 시행한 환자 중 두 식을 각각 정상예측식으로 적용하였을 때 폐확산능 해석이 다른 276명(대상군 A)을 대상으로 하였다. 대상군 A에게 두 식을 각각 적용하였을 때 폐확산능 해석과 임상적 판단과의 일치도를 비교하여 임상적 유용성을 평가하였다. 2. 간질성폐질환 진단의 정확성 비교; 2001년부터 2006년까지 폐조직검사를 시행하여 확진된 간질성폐질환군과 서울아산병원에서 모집한 정상군을 대상으로 하여 정상예측식으로 두 식을 각각 적용하였을 때 폐확산능 해석의 정확도를 비교하였고, 두 식이 차이가 나는지 맥니머의 카이스퀘어 검정을 하였다. 결과: 1. 임상적 판단과의 일치도 비교; 276명을 임상정보를 토대로 폐확산능을 예측하여 분류한 결과 정상 54명, 감소 220명, 불분명이 2명이었다. 예측식으로 박 식과 Burrows 식을 적용하였을 때 임상적 판단과 일치하는 환자는 각각 78%와 22%이었다(p<0.001). 2. 간질성폐질환 진단의 정확성 비교; 박 식은 민감도 90.1%, 특이도 100%이었고 Burrows 식은 민감도 64.2%, 특이도 100%로 민감도가 통계학적으로 유의하게 박 식이 높았다(p<0.001). 결론: 우리나라 정상예측식인 박 식이 외국 정상예측식인 Burrows 식을 정상예측식으로 적용하는 것보다 임상적 유용성이나 간질성폐질환 진단의 민감도에서 더 우월하였다. 향후 폐확산능검사의 정상예측식으로 박 식을 사용해야 할 것으로 사료된다. Background: Park et al. developed the Korean reference equation for the measurement of diffusing capacity in 1985. However, the equation has not been widely used in Korea and foreign reference equations have been popularly used. We intended to compare the clinical usefulness and the accuracy of the the Korean reference equation (Park`s equation) with that of the foreign equation (Burrows` equation) that is commonly used in Korea. Methods: 1. Evaluation of clinical usefulness; Among 1,584 patients who underwent diffusing capacity (DLCO) at the Asan Medical Center from July to December 2006, group A subjects included 276 patients who had different interpretations of DLCO in trials employing Burrows` equation and Park`s equation. Clinical assessment was decided by consensus of two respiratory physicians. In order to evaluate the clinical usefulness of Burrows` equation and Park`s equation, agreement of clinical assessment and DLCO interpretation were measured. 2. Evaluation of accuracy; Group B subjects were 81 patients with interstitial lung disease (ILD) and 39 normal subjects. The 81 ILD patients were diagnosed following a surgical lung biopsy. The accuracy of diagnosing ILD as well as sensitivity and specificity were evaluated according to the use of the reference equations (Burrows` equation and Park`s equation) for DLCO. Results: Agreement between clinical assessment and interpretation of DLCO was 22% for the use of Burrows` equation and 78% for the use of Park`s equation. The sensitivity and specificity of the Burrows` equation for diagnosing ILD were 64.2% and 100%. The sensitivity and specificity of the Park`s equation for diagnosing ILD were 90.1% and 100%. The sensitivity of the Park`s equation for diagnosing ILD was significantly higher than that of Burrows` equation (p<0.001). Conclusion: The Korean reference equation (Park`s equation) was more clinically useful and had higher sensitivity for diagnosing ILD than the foreign reference equation (Burrows` equation). (Tuberc Respir Dis 2008;64:80-86)

Development of a Mobile Application, "Wild Flowers of Bukhansan National Park (version 1.0)", for Identification of Plants in Bukhansan National Park

Kim, Sang-Tae,Lee, Seung-Yeon,Kim, Seung-Chul,Byun, Hye-Won,Lee, Sang-Tae,Kim, Mu-Yeal,Hong, Seok-Pyo,Chung, Young-Jae,Park, Ki-Ryong,Lee, Chung-Hee,Lee, Joong-Ku,Heo, Kyeong-In,Lee, Ji-Ye,Lee, Eun-Je National Science Museum of Korea 2011 Journal of Korean nature Vol.4 No.3

We developed the educational purpose mobile application, named "Wild Flowers of Bukhansan National Park (version 1.0)", aiming for easy identification of wild flowers for students and visitors in the park. When visitors find a flower or part of plant in the park, visitors can search for its name utilizing the pictures and characters provided in their own smartphone mobile devices or tablet PCs. The application provides pictures of wild flowers in the park and character-based searching system based on 12 diagnostic features (e.g., growth form, leaf arrangement, flower symmetry, petal color, petal number, sepal number, etc). We adopted the complete floristic survey of Chung and Lee (1962) and added species that we confirmed their distribution in the park during the development of this application. In summary, number of vascular plants in this park was estimated to be 428 taxa including 100 families, 280 genera, 327 species, 1 subspecies, 50 varieties, and 5 formas. We provided a total of 588 pictures representing 358 taxa and each taxon includes multiple pictures in many cases. Included identification quizzes can be an efficient educational tool as well as fun activity for students and visitors who are learning plant species in Korea. Our next step will include GPS function in the application for indicating visitor's location and for providing previously reported sites of the species that we interested in the map of the park. The future application which includes GPS function will be a valuable tool for the monitoring of rare plants, plant researches related to the climate changes, etc. We currently provide Korean iPhone version only, and English version and both of android versions will be serviced soon.

한국 국립공원 산림생태계의 수목 탄소저장량 평가

이상진,박홍철,박관수,김현숙,이창민,김진원,심규원,최승운,Lee, Sang-Jin,Park, Hong-Chul,Park, Gwan-Soo,Kim, Hyoun-Sook,Lee, Chang-Min,Kim, Jin-Won,Sim, Gyu-Won,Choi, Seung-Woon 한국환경복원기술학회 2022 한국환경복원기술학회지 Vol.25 No.3

The purpose of this study is to quantitatively evaluate the amount of carbon storage for trees in forest ecosystem to support the foundation for carbon neutrality implementation in Korea National Park. It targeted 22 national parks designated and managed as national parks in Korea, and conducted research on forest trees in the terrestrial ecosystem among various natural and ecological carbon sink. The survey and analysis method followed the IPCC guidelines and the National Greenhouse Gas Inventory in Korea. The amount of tree carbon storage in the forest ecosystem of Korea National Park was confirmed to be about 218,505 thousand CO₂-ton and the amount of carbon storage per unit area was 570.8 CO₂-ton per hectare. Compared to 299.7 CO₂-ton per hectare, the average carbon storage per unit area of the entire Korean forest, it was found that about twice as much carbon was stored when assuming the same area. In other words, it means that the tree carbon storage function of the national park is about twice as high as that of the average tree carbon storage function of entire Korean forest. It has great implications in Korea National Park not only provides biodiversity promotion and exploration services as a national protected area, but also performs excellent functions as a carbon sink.

‘경영인의 지혜(Managerial Wisdom)’ 습득을 위한 SSWB 모델의 효과성 연구

김주원(Kim, Joo-Won),박용승(Park, Yong-Seung) 한국경영교육학회 2020 경영교육연구 Vol.35 No.5

[연구목적] 본 연구는 SSWB 모델을 실제 경영학습현장에 대입하기 위해 개발된 프로그램을 소개하고 SSWB 모델의 효과성을 검증하고자 함을 목적으로 한다. [연구방법] 본 연구는 연구자가 근무하고 있는 서울소재 K와 S대학의 책임경영, 기업윤리, 그리고 리더십 수강생 110명을 대상으로 2019년 가을학기에 시행되었다. 측정 도구는 연구자가 개발한 ‘경영인의 지혜’ 이며 사전 사후 검사를 위해 SPSS 25를 활용하여 대응표본 t검증을 실시하였다. [연구결과] 본 연구를 통해 경영인의 지혜에 대한 7개의 하위요인인 고차지식, 자기성찰, 앎의 욕구, 영성 및 초월, 공감, 모순인지 모두 통계적으로 유의미하게 분석되었다. 즉, SSWB 모델을 활용한 프로그램은 경영인의 지혜 수준을 향상시키며 따라서 본 모델의 효과성은 입증되었다. [연구의 시사점] 본 연구는 지금까지의 지혜 학습에 관련된 연구가 관념적인 논증을 거치거나 인터뷰를 통한 질적 연구에 국한되어 있었던 데 반해 실제 양적인 측정을 시도하였다는 것에 큰 의미가 있다. 게다가, 국내 최초로 경영인의 지혜를 개념화하고 측정 도구와 프로그램을 개발한 연구자가 직접 프로그램의 효과성을 검증했다는 것에서도 그 의의를 찾아볼 수 있다. 이는 연구의 방향과 흐름의 일관됨은 물론 같은 주제의 맥락을 하나로 집결시켜 정확한 연구의 본질에 충실할 수 있었음을 의미한다. 또한, 본 연구의 결과로 볼 때 지혜 학습의 가능성이 타당화되었다. 이는 추상적으로 여겨졌던 지혜의 학문적 진입은 물론, 실천적 대입에 대한 촉발제가 되었다는 점에서도 큰 의의를 지닌다. [Purpose] The purpose of this study is to first introduce the program developed to apply the SSWB model to the actual management learning site. The purpose is to verify the effectiveness of the SSWB model through comparative analysis before and after implementation of the program. [Methodology] The study was conducted in the fall semester of 2019 on 110 students of responsible management, management ethics, and leadership at K and S universities in Seoul where researchers work. For this study, pretest-posttest was conducted using the ‘Management Wisdom’ measurement tool developed by the researcher, and the analysis and verification were conducted using SPSS 25. [Findings] As a result, all factors resulted in statistically significant results. In other words, the SSWB model can be interpreted as improving the level of management wisdom. Therefore, it has been verified that this is a useful program for learning the wisdom of managers. [Implications] This study is significant in that the research related to learning wisdom has been limited to qualitative research through interview or through ideological reasoning so far, while actual quantitative measurement has been attempted. Moreover, the significance of the program can be found in the fact that the researchers who have conceptualized the wisdom of managers and developed measurement tools and programs for the first time in Korea have personally verified the effectiveness of the program. This means that the direction and flow of research are consistent, as well as the context of the same subject can be brought together to be faithful to the exact nature of the study. Also, the possibility of learning wisdom has been justified. This is of great significance not only in the academic entry of wisdom, which was considered abstract, but also in the fact that it was the trigger for practical college entrance.

Identification and characterization of Metarhizium majus isolated from the edible insect Protaetia brevitarsis in Korea

Kwak Kyu‐Won,Aktaruzzaman Md.,Kim Eunsun,Kim Sun Young,Hong Seung‐Beom,Park Ji Yeong,Park Kwanho,Koo Bonwoo,Kim Yong‐Soon 한국곤충학회 2021 Entomological Research Vol.51 No.12

Edible insects are considered novel protein sources, and the edible insect breeding industry is growing rapidly. However, fungal pathogens are a major threat to this industry, and appropriate identification of causative agents is important for the development of antifungal agents. In 2019, strains of the fungal pathogen Metarhizium were isolated from infected second and third instar larvae of the economically important edible insect Protaetia brevitarsis, which were collected from separate farms in Gimhae-si, Gyeongsangnam-do, Korea. Based on morphological characteristics and multilocus phylogenetic analysis of the internal transcribed spacer region, and 50-region of gene encoding the 1-α elongation factor, the fungal strains were identified as Metarhizium majus. Here, we report the detailed phylogenetic characteristics and morphological description of this species, which is prevalent in Korea. Identification of the principal disease-causing agent can help develop disease prevention and antifungal strategies for application in edible insect-rearing farms.

경관형용사 분석법을 활용한 국립공원 마을 유형별 경관 선호도에 관한 연구

심규원(Sim, Kyu-Won),박헌춘(Park, Heon-Choon),남승민(Nam, Seung-Min) 한국산림휴양복지학회 2021 한국산림휴양학회지 Vol.25 No.2

본 연구의 목적은 경관형용사 분석법을 활용하여 국립공원 내에 있는 마을의 유형별로 경관 선호도에 영향을 주는 경관 이미지를 규명하고자 하였다. 분석 결과, 경관 선호도에 영향을 주는 경관 요인은 마을 유형별로 차이가 있으며, 마을 경관 디자인 가이드라인 수립 시 고려해야할 경관 이미지는 아래와 같다. 첫째, 농촌형 마을의 전원성에만 국한된 형용사는 ‘자연스러운’, ‘풍요로운’, ‘전통적인’, ‘정적인’으로 산촌형 및 어촌형 마을보다는 가장 일반적이고 전통적인 이미지의 고정관념을 지니고 있다고 볼 수 있다. 둘째, 어촌형 마을의 전원성 중 가장 큰 차이를 보이는 부분은 어촌형 마을에만 도출된 형용사 ‘자유로운’이다. 셋째, 산촌형 마을의 형용사적 차이를 보이는 항목은 일반적으로 산의 특성을 반영하는 ‘울창한’, ‘깊은’ 두 단어이다. 도출된 결과를 바탕으로 국립공원 내 존치되어 있는 마을의 정주여건 개선과 공원자원으로서 활용성 제고하고, 공원 내 주민의 삶의 질 향상과 공원 내 마을 이미지를 개선하는데 활용할 수 있을 것이다. The purpose of this study was to identify landscape images that affect landscape preferences by type of village in the national park by using the landscape adjective analysis. As a result of the analysis, the landscape factors that affect landscape preferences vary by village type, and the landscape image to consider when establishing the village landscape design guidelines is as follows. First, adjectives limited to the rural village’s rural originality are ‘natural’, ‘rich’, ‘traditional’ and ‘static’, which have the most common and traditional image stereotypes rather than mountain and seaside villages. Second, the biggest difference among the rural characteristics of seaside villages is the adjective‘free’derived only from this village. Third, the two words that generally reflect the characteristics of the mountain village are ‘dense’ and ‘deep’. Based on the results, it will be able to improve the residential conditions of villages in the national park and improve their utilization as park resources, the quality of life in the park, and the village image in the park.

국립공원 내 공중화장실에 대한 탐방객 인식에 관한 연구 : 지리산국립공원을 중심으로

이호(Ho Lee),이승록(Seung-Rok Lee),박기현(Ki-Hyun Park),박은희(Eun-Hee Park),차진열(Jin-Yeol Cha),심규원(Kyu-Won Sim),강동원(Dong-Won Gang) 국립공원연구원 2011 국립공원연구지 Vol.2 No.1

본 연구는 공원 내 공중화장실에 대한 탐방객의 인식이 만족도에 미치는 영향을 규명하고자 수행하였다. 현지 설문조사는 지리산국립공원의 성삼재와 중산리 일원을 방문한 탐방객 292명을 대상으로 이루어졌다. 분석결과, 공중화장실의 관리상태, 외관, 디자인 요인 모두 탐방객의 공중화장실 이용 만족에 영향을 주는 것으로 분석되었으며, 그 중 관리상태가 가장 큰 영향을 주는 것으로 나타났다. 따라서 탐방객의 인식과 만족을 향상시키기 위해서는 공중화장실의 청결한 관리 및 유지, 악취 방지, 소모품의 원활한 공급 등에 우선순위를 두어 집중적으로 관리할 필요가 있는 것으로 나타났다. This study conducts to investigate the relationship between visitors’ perception and satisfaction. Data of 292 visitors were collected from Seongsamjae and Jungsanri in Jirisan National Park. The results showed that maintenance, outlook and design of public toilet are positively related to visitors’ satisfaction. One of the major research findings is that the maintenance of public toilet most affects visitors’ satisfaction. Therefore, the park managers need to put great emphasis on the cleanliness and maintenance, preventing stinks, consumable supplies of public toilet in order to enhance visitors’ perception and satisfaction.

Photonic Crystals: Integration of Colloidal Photonic Crystals toward Miniaturized Spectrometers (Adv. Mater. 9/2010)

Kim, Shin-Hyun,Park, Hyo Sung,Choi, Jae Hoon,Shim, Jae Won,Yang, Seung-Man WILEY-VCH Verlag 2010 Advanced materials Vol.22 No.9

<B>Graphic Abstract</B> <P>The cover shows a schematic illustration of patterned colloidal photonic crystals with different bandgap positions. When an unknown light source impinges on the patterned photonic crystals, the light information can be identified from the reflection intensity profile of the constituent photonic crystals. The two optical microscopy images and background image display integrated photonic crystals with 20 different bandgaps spanning the entire visible range, and the SEM image shows the cross-section of the photonic crystal stripes, as reported by Shin-Hyun Kim, Seung-Man Yang, and co-workers on p. 946. <img src='wiley_img_2010/09359648-2010-22-9-ADMA201090022-content.gif' alt='wiley_img_2010/09359648-2010-22-9-ADMA201090022-content'> </P>

건조방법에 따른 하수슬러지 건조속도 비교 및 경제성 평가

박성훈 ( Seong-hun Park ),김한석 ( Han-seok Kim ),이승원 ( Seung-won Lee ) 한국환경기술학회 2021 한국환경기술학회지 Vol.22 No.3

In this study investigated the effect of the chemical injection ratio on the drying rate of sewage sludge. Then the economic efficiency was evaluated by comparing the results of the NIR(near infrared ray) and microwave drying rate test. The near infrared ray device showed drying speed of 31.9 %, 28.1 %, and 27.1 % at polymer dose ratio of 8 %, 10 %, and 12 %, respectively. Compared to 8 % polymer dose ratio, polymer dose ratio of 10 % and 12 % showed differences of 8.0 % and 13.6 % respectively, suggesting that drying efficiency decreased as polymer dose ratio increased. The microwave device showed drying speed of 26.2 %, 24.3 %, 21.3 % at polymer dose ratio of 8 %, 10 %, and 12 %, respectively. Compared to 8 % polymer dose ratio, polymer dose ratio of 10 % and 12 % showed differences of 5.2 % and 13.4 % respectively, suggesting that drying efficiency decreased as polymer dose ratio increased. Drying evaluation estimated the treatment cost of near infrared ray device at optimal dose ratio(6 %) was 35,591 won/ton while that of actual dose ratio(8 %) was 38,928 won/ton. Drying evaluation estimated treatment cost of microwave device at optimal polymer dose ratio(6 %) and at 8 % to be 16,683 won/ton and 18,352 won/ton, respectively.