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Soyun Cho,Chong-Hyun Won,이동훈,Min-Jung Lee,Serah Lee,Seung-Ho So,Seong-Kye Lee,Bon-Suk Koo,Na-Mi Kim,정진호 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.6
Red ginseng contains many bioactive constituents, including various ginsenosides that are believed to have antioxidant, immunostimulatory, and anti-aging activities. Yet, no controlled human study has explored its effects on photoaged skin. This study determined whether long-term intake of a red ginseng extract-containing Torilus fructus and Corni fructus mixture reduces facial wrinkles and increases collagen synthesis in human skin. Healthy female volunteers over 40 years of age were randomized in a double-blind fashion to receive either red ginseng extract-containing herbal mixture at 3g/day or placebo for 24 weeks. Facial wrinkles, elasticity, epidermal water content, erythema, and pigmentation were measured objectively. Facial skin samples were taken before and after treatment, and real-time polymerase chain reaction and immunohistochemical analyses were undertaken for expression of type I procollagen, matrix metalloproteinase (MMP)-9, and fibrillin-1, which are wrinkle-related biochemical markers. A total of 82 subjects completed the study. Facial wrinkles were significantly improved, type I procollagen gene and protein expression was increased, MMP-9 gene induction was prevented, and fibrillin-1 fiber length was elongated only in the treatment group. No changes were seen in the facial elasticity, epidermal water content, facial erythema and pigmentation, and epidermal thickness in either group. Thus a red ginseng extract-containing Torilus fructus and Corni fructus mixture improves facial wrinkles, a clinical sign of photoaging, and this improvement is associated with biochemical and histological evidence of increased collagen synthesis in the dermis. These results substantiate the alleged beneficial effects of red ginseng on photoaging and support its use as an effective “beauty food.”
Min Ji KIM,Stacy Simai REGINALD,Hyeryeong LEE,Serah CHOI,Basit SHARIF,In Seop CHANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Over the last century, the accumulation of carbon dioxide (CO₂) which accounts for the largest portion of greenhouse gases in the atmosphere has provoked extreme global warming and environmental issues. To tackle these issues, carbon capture, utilization and storage (CCUS) technology has received considerable attention through all the related areas. In the field of CO₂ conversion having an effect on CO₂ valorization to valuable chemicals as well as removal, biocatalysts such as enzyme are promising due to their high specificity to substrate and ability of catalysis at mild conditions. Herein, we focused on CO₂ reducing enzyme, formate dehydrogenase (EC 1.17.1.9) (FDH) which can reversibly catalyze CO₂ reduction and formate oxidation. FDHs existed in a diverse array of organisms can be divided into NAD⁺-dependent and metal-dependent FDH. While metal-dependent FDH is highly efficient for CO₂ reduction to formate but requires strict anaerobic conditions, NAD⁺-dependent FDH can be easily handled, but NADH are required as a natural cofactor. In this study, we attempted to investigate the availability of Candida methylica FDH(cmFDH) for electroenzymatic CO₂ reduction to formate. Solid-binding peptide (SBP) was introduced at either N- terminus (gbp(N)-FDH) or C- terminus (FDH-gbp(C)) via protein engineering to develop a stable enzyme immobilization at the enzyme-electrode interface. The recombinant FDH and the two synthetic FDHs are characterized for their biocatalytic activity. The results indicated that GBP fusion CmFDHs retain or improve their enzyme activities. Cyclic voltammetry (CV) result shows that the electrons can be directly transferred from FDHs to the electrode surface and vice versa for formate oxidation and CO₂ reduction in the absence of NADH.
Jin, Seon-Pil,Li, Zhenyu,Choi, Eun Kyung,Lee, Serah,Kim, Yoen Kyung,Seo, Eun Young,Chung, Jin Ho,Cho, Soyun Elsevier 2018 Journal of dermatological science Vol.91 No.2
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Particulate matter (PM) is an integral part of air pollution, which is a mixture of particles suspended in the air. Recently, it has been reported that PM is associated with increased risks of skin diseases, especially atopic dermatitis in children. However, it is unclear if PM directly goes into the skin and what mechanisms are involved in response to PM.</P> <P><B>Objective</B></P> <P>To see whether PM could penetrate into the barrier-disrupted skin, produce reactive oxygen species (ROS), and elicit an inflammatory response.</P> <P><B>Methods</B></P> <P>We collected PMs during a winter in Seoul and used cultured keratinocytes for <I>in vitro</I> study and tape-stripped BALB/c mice for <I>in vivo</I> study.</P> <P><B>Results</B></P> <P>Keratinocyte cytotoxicity increased in a dose-dependent manner by PM treatment. IL-8 and MMP-1 mRNA expression and protein levels were significantly increased compared to control by qPCR and ELISA, respectively. Cellular ROS production was increased by PM treatment, and antioxidant N-acetyl cysteine pretreatment prevented induction of inflammatory cytokines IL-8 and MMP-1. In PM-treated keratinocytes, electron-dense subcellular particles were observed by transmission electron microscopy. PM was observed inside hair follicles in both intact and barrier-disrupted skin <I>in vivo</I>. Additionally, intercellular penetration of PM was seen in the barrier-disrupted skin. Repeated PM application induced epidermal thickening and dermal inflammation with neutrophil infiltration. Finally, N-acetyl cysteine could ameliorate skin inflammation induced by PM application.</P> <P><B>Conclusion</B></P> <P>PM penetrates into the barrier-disrupted skin, causing inflammation, demonstrating detrimental effects in the skin.</P> <P><B>Highlights</B></P> <P> <UL> <LI> It is unclear if particulate matter (PM) directly goes into the skin. </LI> <LI> We provide visual images of PM penetrating into epidermis in the barrier-disrupted skin. </LI> <LI> Repeated PM application leads to cutaneous inflammation via ROS-dependent manner. </LI> <LI> It may have clinical implications especially for patients with deficient skin barrier including atopic dermatitis, diabetics. </LI> </UL> </P>
P003 : Blood type B-specific difference in skin phenotypes of Korean women
( Jang Hee Oh ),( Inn Gyung Oh ),( Chi Hyun Park ),( Min Kyeong Shin ),( Serah Lee ),( Dong Hun Lee ),( Mira Choi ),( Seon Pil Jin ),( Soyun Cho ),( Jin Ho Chung ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: ABO blood group antigen expression was found on many tissues including the granular of the epidermis. Objectives: To investigate the blood type-specific difference in normal skin phenotypes in Korean women. Methods: Skin hydration, transepidermal water loss (TEWL), wrinkle depth, elasticity, or skin erythema/melanin index, were analyzed according to the blood type. Results: The skin hydration at buttock skin showed significantly lower in B blood type individuals than non-blood type B individuals of same young age range (20~49 yr, mean age; 34.0±9.3 yr, n=40), but TEWL of them showed no difference. However, the skin hydration and TEWL in old age range (54~84 yr, mean age; 70.4±6.2 yr, n=126) showed no B blood type-specific difference in inner-arm skin. The eye wrinkle depth in old B blood type individuals also was observed to be significantly deeper than non-B blood type individuals of same old age range. Deeper eye wrinkle was also observed in O blood type than A or AB blood type, but seemed less than B blood type. However, their skin elasticity showed no difference. The melanin index in old B blood type individuals also showed less measurement, which means bright skin color, than non-B blood type individuals, while the erythema index did not. Conclusion: In conclusion, we found that B blood type individuals probably has most distinct skin phenotypes, including less hydration, deeper eye wrinkle, and bright skin color in Korean women.